Journal of Fermentation Technology最新文献_第3页

l-serine production from methanol and glycine with an immobilized methylotroph 固定化甲基化菌催化甲醇和甘氨酸生产l-丝氨酸

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90107-0

Pramote Sirirote, Tsuneo Yamane, Shoichi Shimizu

{"title":"l-serine production from methanol and glycine with an immobilized methylotroph","authors":"Pramote Sirirote, Tsuneo Yamane, Shoichi Shimizu","doi":"10.1016/0385-6380(88)90107-0","DOIUrl":"10.1016/0385-6380(88)90107-0","url":null,"abstract":"<div>l-Serine production from methanol and glycine was attempted using immobilized resting cells of a methylotroph, Protomonas extorquens NR 1, under automatically controlled conditions. A Ca-alginate system was selected. The conditions for l-serine formation were optimized at 30°C. A concentration of glycine 100 g·l−1 which was the optimum concentration for l-serine production by free resting cells was used in the reaction mixture. The optimum concentrations of methanol and dissolved oxygen were 20 g·l−1 and 5 ppm, respectively. Under the optimum conditions, 11.3 g·l−1 of l-serine was produced within 36 h. The selectivities (mole of l-serine/mole of substrate consumed) of l-serine from methanol and glycine were 4.5% and 95.1%, respectively. The size of gel beads affected the l-serine formation rate. The initial rate of l-serine formation decreased with an increase in the size of beads. However, the l-serine formation rate increased at elevated concentrations of dissolved oxygen, even with large sized beads. This result implies that the oxygen diffusion inside the gel beads limited the l-serine formation rate. The observed effectiveness factor of the immobilized cells could be estimated by the theoretical effectiveness factor of the zero-order reaction with respect to the dissolved oxygen.Repeated use was not feasible without reactivation of the immobilized cells. Reusability was examined by reactivation of the immobilized resting cells in appropriate media for 12 h. The reactivated immobilized resting cells were used again in the next cycle. By this procedure, several cycles of l-serine formation were made possible.</div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 3","pages":"Pages 291-297"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90107-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86565386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Isolation of a yeast growing on sardine oil and its characteristics 沙丁鱼油酵母的分离及特性研究

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90104-5

Yasuhide Ota, Yasushi Kushida

引用次数: 5

Analysis of a promoter-like region in Flavobacterium which controls 6-aminohexanoic acid linear oligomer hydrolase gene expression in Escherichia coli 黄杆菌中控制大肠杆菌6-氨基己酸线性低聚物水解酶基因表达的启动子样区域分析

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90080-5

Mitsuo Okazaki , Tomohiro Uemura , Yasumoto Nishimori , Kiichiro Wakamatsu , Makoto Shimosaka , Hideki Saito , Seiji Negoro , Hirosuke Okada

引用次数: 5

Product inhibition and its removal on josamycin fermentation by Streptomyces narbonensis var. josamyceticus 纳邦链霉菌发酵josamycin的产物抑制及去除

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90089-1

Hideo Eiki , Hiroshi Gushima, Takeshi Saito, Hitoshi Ishida, Yoshihiko Oka, Takashi Osono

引用次数: 23

Cell wall mutants of Saccharomyces cerevisiae with increased digestibility by cell wall lytic enzymes and protein extractability 利用细胞壁裂解酶提高消化率和蛋白质提取率的酿酒酵母细胞壁突变体

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90100-8

Tasuku Nakajima , Reiko Konno , Hiroshi Nishihara , Kazuo Matsuda

引用次数: 2

Conversion of 5′-inosinic acid to inosine by Streptomyces aureus 金链霉菌将5′-肌苷酸转化为肌苷

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90092-1

Krung Hyun Sohn , Chan Yong Lee , Ke Ho Lee , Young Je Cho , Jae Heung Lee

引用次数: 0

Mechanistic analysis of xanthan gum production in a stirred tank 搅拌槽生产黄原胶的机理分析

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90115-X

Hitoshi Funahashi , Koh-Ichi Hirai , Toshiomi Yoshida , Hisaharu Taguchi

{"title":"Mechanistic analysis of xanthan gum production in a stirred tank","authors":"Hitoshi Funahashi , Koh-Ichi Hirai , Toshiomi Yoshida , Hisaharu Taguchi","doi":"10.1016/0385-6380(88)90115-X","DOIUrl":"10.1016/0385-6380(88)90115-X","url":null,"abstract":"<div>The overall effect of agitation on xanthan gum production by Xanthomonas campestris ATCC13951 in a stirred vessel was mechanistically analyzed considering local variation of the specific production rate due to variation of shear stress in the vessel. The whole liquid volume in a fermentor was roughly divided into three regions; the micromixing region around the impeller with high shear stress, the macromixing region dominated by a circulating flow and the stagnant region. The value of the shear rate was first ascertained by experiments in order to obtain a picture of shear rate variation in a radial direction from the impeller, and the equivalence between the volumes of the high shear stress region and micromixing region was confirmed. The shear stress obtained using a correlation between the shear rate at the impeller tip and Reynolds number of Wichterle et al. was used as a representative of the shear stress in the micromixing region, and the shear stress estimated by use of an empirical correlation between the average shear rate in a fermentor and agitation speed derived by Metzner et al. was adopted as a representative of the shear stress in the macromixing region. The information about the circulation time distribution was also used to take into account oxygen deficiency during circulation of liquid elements in the macromixing region, considering that oxygen from the gas phase was supplied mainly in the high shear region. The calculated values of xanthan gum concentrations which were obtained by the proposed simulation method agreed well with the experimental data in the time course of xanthan gum production at various agitation speeds. Experimental results of the relationship between the overall specific production rate and ND (N, agitation speed, and D, impeller diameter) was also verified by the proposed method.</div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 3","pages":"Pages 355-364"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90115-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82428365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Inulinase from Chrysosporium pannorum

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90088-X

Rong Xiao, Masatoshi Tanida, Shoichi Takao

引用次数: 39

Perfusion culture of hybridoma cells recycling higher-molecular-weight components 杂交瘤细胞循环高分子量组分的灌注培养

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90143-4

Michiyuki Tokashiki, Kimihiko Hamamoto, Yoshiharu Takazawa

引用次数: 3

Magnesium requirement for biological removal of phosphate by activated sludge 活性污泥生物去除磷酸盐所需镁

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90070-2

Hiroshi Imai , Kazuo Endoh , Takeshi Kozuka

{"title":"Magnesium requirement for biological removal of phosphate by activated sludge","authors":"Hiroshi Imai , Kazuo Endoh , Takeshi Kozuka","doi":"10.1016/0385-6380(88)90070-2","DOIUrl":"10.1016/0385-6380(88)90070-2","url":null,"abstract":"<div>The effects of magnesium on excess uptake of phosphate in an aerobic-anaerobic activated sludge process were examined by the fill and draw procedure. The alternation of anaerobic and aerobic conditions in one cycle of fill and draw process was varied many ways.The presence of sufficient magnesium was necessary for uptake of excess phosphate. When sludge contained more phosphorus than the upper limit of phosphorus content in the usual aerobic activated sludge, 2.5% by weight, magnesium was also contained in more than an ordinary amount (0.5%). Their contents in the sludge at the end of each cycle of the process were correlated with each other by a linear equation with the correlation coefficient of 0.99. When magnesium concentration was insufficient for the uptake of excess phosphate, its concentration in the treated water was of the order of 0.1 mg/l.In the first anaerobic period both phosphate and magnesium were released, and in a successive aerobic period they were taken up again. The weight ratio of differential amounts of phosphorus and magnesium released or taken up changed with time in one cycle.Dynamic behaviors of phosphate and magnesium removal against the step change of feed magnesium concentration also showed a stoichiometric relationship supporting the correlation equation above mentioned.</div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 6","pages":"Pages 657-666"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90070-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84920735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25