Journal of Fermentation Technology最新文献_第3页

l-serine production from methanol and glycine with an immobilized methylotroph 固定化甲基化菌催化甲醇和甘氨酸生产l-丝氨酸

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90107-0

Pramote Sirirote, Tsuneo Yamane, Shoichi Shimizu

{"title":"l-serine production from methanol and glycine with an immobilized methylotroph","authors":"Pramote Sirirote, Tsuneo Yamane, Shoichi Shimizu","doi":"10.1016/0385-6380(88)90107-0","DOIUrl":"10.1016/0385-6380(88)90107-0","url":null,"abstract":"<div>l-Serine production from methanol and glycine was attempted using immobilized resting cells of a methylotroph, Protomonas extorquens NR 1, under automatically controlled conditions. A Ca-alginate system was selected. The conditions for l-serine formation were optimized at 30°C. A concentration of glycine 100 g·l−1 which was the optimum concentration for l-serine production by free resting cells was used in the reaction mixture. The optimum concentrations of methanol and dissolved oxygen were 20 g·l−1 and 5 ppm, respectively. Under the optimum conditions, 11.3 g·l−1 of l-serine was produced within 36 h. The selectivities (mole of l-serine/mole of substrate consumed) of l-serine from methanol and glycine were 4.5% and 95.1%, respectively. The size of gel beads affected the l-serine formation rate. The initial rate of l-serine formation decreased with an increase in the size of beads. However, the l-serine formation rate increased at elevated concentrations of dissolved oxygen, even with large sized beads. This result implies that the oxygen diffusion inside the gel beads limited the l-serine formation rate. The observed effectiveness factor of the immobilized cells could be estimated by the theoretical effectiveness factor of the zero-order reaction with respect to the dissolved oxygen.Repeated use was not feasible without reactivation of the immobilized cells. Reusability was examined by reactivation of the immobilized resting cells in appropriate media for 12 h. The reactivated immobilized resting cells were used again in the next cycle. By this procedure, several cycles of l-serine formation were made possible.</div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 3","pages":"Pages 291-297"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90107-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86565386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Isolation of a yeast growing on sardine oil and its characteristics 沙丁鱼油酵母的分离及特性研究

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90104-5

Yasuhide Ota, Yasushi Kushida

引用次数: 5

Analysis of a promoter-like region in Flavobacterium which controls 6-aminohexanoic acid linear oligomer hydrolase gene expression in Escherichia coli 黄杆菌中控制大肠杆菌6-氨基己酸线性低聚物水解酶基因表达的启动子样区域分析

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90080-5

Mitsuo Okazaki , Tomohiro Uemura , Yasumoto Nishimori , Kiichiro Wakamatsu , Makoto Shimosaka , Hideki Saito , Seiji Negoro , Hirosuke Okada

引用次数: 5

Product inhibition and its removal on josamycin fermentation by Streptomyces narbonensis var. josamyceticus 纳邦链霉菌发酵josamycin的产物抑制及去除

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90089-1

Hideo Eiki , Hiroshi Gushima, Takeshi Saito, Hitoshi Ishida, Yoshihiko Oka, Takashi Osono

引用次数: 23

Cell wall mutants of Saccharomyces cerevisiae with increased digestibility by cell wall lytic enzymes and protein extractability 利用细胞壁裂解酶提高消化率和蛋白质提取率的酿酒酵母细胞壁突变体

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90100-8

Tasuku Nakajima , Reiko Konno , Hiroshi Nishihara , Kazuo Matsuda

引用次数: 2

The structure of high molecular weight dextrins obtained from potato starch by treatment with Bacillus macerans enzyme 用芽孢杆菌酶处理马铃薯淀粉制备的高分子量糊精的结构

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90042-8

Yoji Kato , Katsuhiko Mikuni , Kozo Hara , Hitoshi Hashimoto , Tasuku Nakajima , Shoichi Kobayashi , Keiji Kainuma

{"title":"The structure of high molecular weight dextrins obtained from potato starch by treatment with Bacillus macerans enzyme","authors":"Yoji Kato , Katsuhiko Mikuni , Kozo Hara , Hitoshi Hashimoto , Tasuku Nakajima , Shoichi Kobayashi , Keiji Kainuma","doi":"10.1016/0385-6380(88)90042-8","DOIUrl":"10.1016/0385-6380(88)90042-8","url":null,"abstract":"<div>Bacillus macerans enzyme (BME)-derived high molecular weight dextrins, which are by-products in the course of the industrial production of cylodextrins, were isolated and their chemical structures were characterized.Dextrin I was obtained in a yield of about 24% from BME-hydrolyzate (a mixture of dextrin and cylodextrins, 50% each) of potato starch by fractionation with an ultrafiltrator having a membrane of cut-off molecular weight 2.0 × 104. Dextrin II was obtained in a yield of about 15% from BME-hydrolyzate (a mixture of dextrins and cyclodextrins, 70 : 30) of Dextrin I by the same method.Dextrin I and II consisted of dextrin having molecular weights over 20 × 106 and dextrins having molecular weights 4 × 103−1 × 105 in the ratio of 80 : 12 and 66: 15, respectively.The results of hydrolysis by β-amylase and methylation analysis indicated that the average, exterior and interior chain lenghts of the dextrins having molecular weights over 20 × 106 and 4 × 103−1 × 105 from Dextrin I were 16.5, 8.2 and 7.3, and 11.5, 6.9 and 3.6, respectively, than those from Dextrin II were 13.6, 4.7 and 9.9, and 10.4, 5.1 and 4.3, respectively.</div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 2","pages":"Pages 159-166"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90042-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72925731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Potential availability of anaerobic treatment with digester slurry of animal waste for the reclamation of acid mine water containing sulfate and heavy metals 动物粪便沼液厌氧处理含硫酸盐和重金属矿山酸性水的可行性研究

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90128-8

Katsuji Ueki, Keiko Kotaka, Kenji Itoh, Atsuko Ueki

{"title":"Potential availability of anaerobic treatment with digester slurry of animal waste for the reclamation of acid mine water containing sulfate and heavy metals","authors":"Katsuji Ueki, Keiko Kotaka, Kenji Itoh, Atsuko Ueki","doi":"10.1016/0385-6380(88)90128-8","DOIUrl":"10.1016/0385-6380(88)90128-8","url":null,"abstract":"<div>The use of an anaerobic digester slurry of cattle waste for the reclamation of acid mine water was examined. When the digester slurry was mixed with acid mine water, anaerobic digestion, including sulfate reduction and methanogenesis, was enhanced. In the mixture of acid mine water and the digester slurry, sulfate reduction proceeded without diminishing methanogenesis. The digester slurry and its supernatant (SDF-sup) showed a significant capacity to act as a strong alkaline reagent, and the pH of the acid mine water was markedly elevated by the addition of the digester slurry of SDF-sup even at the low ratio of 1% (v/v). Precipitation of heavy metals in the acid mine water occurred as the pH was elevated by the addition of SDF-sup. When the digester slurry was added at the ratio of 5% (v/v) to acid mine water which had been pretreated with SDF-sup, the rate of sulfate reduction increased with increasing the concentration of sulfate in the mixture up to about 1,400 mg·l−1. In acid mine water pretreated with SDF-sup and supplemented with the digester slurry at the ratio of 5% (v/v), the maximum amount of sulfate reduced within 20 d of incubation was about 1,000 mg·l−1, and the maximum rate of sulfate reduction was about 120 mg SO42−·l−1·d−1.</div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 1","pages":"Pages 43-50"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90128-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74788578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Hydrolysis of acid-treated protein by a preparation of proteases 水解:用蛋白酶制备酸处理过的蛋白质的水解

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90086-6

Tadanobu Nakadai, Seiichi Nasuno

引用次数: 3

Isolation and cultural conditions of galactooligosaccharide producing yeast Cryptococcus laurentii 产半乳糖低聚糖酵母劳伦隐球菌的分离与培养条件

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90021-0

Kataro Ohtsuka, Shuichi Oki, Osamu Ozawa, Takatsugu Uchida

引用次数: 13

Factors affecting the synthesis of the n-terminal methionine-free molecule of recombinant human interleukin-2 by Escherichia coli 影响大肠杆菌合成重组人白细胞介素-2 n端无蛋氨酸分子的因素

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI: 10.1016/0385-6380(88)90045-3

Shigeru Fujimoto, Masanori Nakatsu, Koichi Kato, Kazuaki Kitano

引用次数: 7