{"title":"Stability of plasmid-bearing microorganisms in batch and continuous cultures","authors":"Pi-Chao Wang, Hisao Ohtake, Kiyoshi Toda","doi":"10.1016/0385-6380(88)90131-8","DOIUrl":"10.1016/0385-6380(88)90131-8","url":null,"abstract":"<div><p>The stability of five microbial strains bearing a domestic and/or exotic plasmid was investigated in continuous culture to obtain basic information on the fate of genetically engineered microorganisms released in the natural environment.</p><p>The three strains with an exotic plasmid were constructed by the conjugal or mobilized transfer of conjugative plasmid R100-1 and non-conjugative plasmid RSF2124. Plasmid loss occurred only at the declining growth phase of batch culture of the transconjugants; the ratio of plasmid-free cells was 40–50% at the end of the culture, independent of the strains, whereas the plasmid in the native host cells was maintained at almost 100% of stability.</p><p>In continuous culture of the transconjugant cells, the population ratio of plasmid-free cells at the pseudo-steady state was between 5–80% depending on the strain. The plasmid-bearing cells were not washed out of the continuous fermentor for 43 generations but maintained their quasi-stable concentration with some degree of oscillation. Simultaneous loss and retransfer of the plasmid from and to its host cells is suggested for the explanation.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 1","pages":"Pages 63-70"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90131-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86636879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Grain spirits making with use of uncooked maize and its flavor components","authors":"Akira Nose, Kimio Iwano, Satoshi Shinoki, Mitsuo Hirai, Kiyoshi Yoshizawa","doi":"10.1016/0385-6380(88)90076-3","DOIUrl":"10.1016/0385-6380(88)90076-3","url":null,"abstract":"","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 6","pages":"Pages 685-686"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90076-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"102399493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oxidation of Hydroxylamine by Pseudomonas putida","authors":"","doi":"10.1016/0385-6380(88)90097-0","DOIUrl":"https://doi.org/10.1016/0385-6380(88)90097-0","url":null,"abstract":"","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 5","pages":"Page 596"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90097-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136914361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Author and subject index","authors":"","doi":"10.1016/0385-6380(88)90098-2","DOIUrl":"https://doi.org/10.1016/0385-6380(88)90098-2","url":null,"abstract":"","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 5","pages":"Pages I-II"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90098-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136914362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Denitrifying and photoheterotrophic growth of Rhodobacter sphaeroides S under anaerobic-dark and -light conditions","authors":"Ken Sasaki , Hiroyuki Morii , Yoshinori Nishizawa , Shiro Nagai","doi":"10.1016/0385-6380(88)90125-2","DOIUrl":"10.1016/0385-6380(88)90125-2","url":null,"abstract":"<div><p>Denitrifying growth characteristics of <em>Rhodobacter shaeroides</em> S were examined in batch and continuous cultures under anaerobic-dark and -light conditions. Growth yield based on the electron equivalent, <em>Y</em><sub>eq.</sub>, in anaerobic-dark conditions was 2–3 times lower than that in aerobic-dark conditions (aerobic-heterotropic growth). Under anaerobic-light conditions, denitrifying growth was observed with photosynthetic growth, but decreased with the increase of light intensity. The value of <em>Y</em><sub>eq.</sub> in anaerobic-light conditions increased with the increase of light intensity. This phenomenon could be explained as the change of the fraction of cell mass grown by denitrification and photosynthesis in the total cell mass from the energetic analysis.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 1","pages":"Pages 27-32"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90125-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84317920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mass and energy balance in glucose and lactose metabolism in Fusarium oxysporum","authors":"I. Kiran Kumar, Saroj Mishra","doi":"10.1016/0385-6380(88)90013-1","DOIUrl":"10.1016/0385-6380(88)90013-1","url":null,"abstract":"<div><p>The fungus <em>Fusarium oxysporum</em> was cultivated on glucose and lactose medium in a 2 l bioreactor to evaluate its carbon conversion efficiency. The initial optimization of culture conditions indicated NH<sub>4</sub>NO<sub>3</sub> or NH<sub>4</sub>Cl as the best N source at a C/N ratio between 1:4.5 to 1:11.5 at 1% glucose concentration. The pattern of allocation of the substrate carbon and energy into the production of biomass, carbon dioxide, and heat during growth was identified by applying mass and energy balance equations. It is indicated that glucose-containing substrates are better for SCP production from the organism than substrates with lactose.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 4","pages":"Pages 449-453"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90013-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80202947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of seeding on thermophilic composting of household organic waste","authors":"Kiyohiko Nakasaki, Tetsuo Akiyama","doi":"10.1016/0385-6380(88)90127-6","DOIUrl":"10.1016/0385-6380(88)90127-6","url":null,"abstract":"<div><p>The effects of seeding on the thermophilic composting of household organic waste were examined by measuring the changes in CO<sub>2</sub> evolution rate, temperature, conversion of volatile matter, pH, and microbial succession in the composting reaction. The courses of the above values differed substantially between the seeded and nonseeded experiments. The rises in temperature and pH, as well as the appearance of large peaks in the rate of CO<sub>2</sub> evolution occurred at earlier times in the seeded composting than in the nonseeded one. Furthermore, the final conversion of volatile matter was greater in the seeded composting than in the nonseeded one. The analysis of microbial succession indicated that the seeded composting was accelerated both in mesophilic and thermophilic stages.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 1","pages":"Pages 37-42"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90127-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77841870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structural alteration of cell wall polysaccharides from Saccharomyces cerevisiae mutants","authors":"Tasuku Nakajima , Hiroshi Nishihara , Yogo Chiba , Kazuo Matsuda","doi":"10.1016/0385-6380(88)90101-X","DOIUrl":"10.1016/0385-6380(88)90101-X","url":null,"abstract":"<div><p>The chemical structures of cell wall mannans and β-glucans from two <em>Saccharomyces cerevisiae</em> mutants, N3 and N3-8, which have morphological alterations and fragile cell walls, were compared with a wild type strain. Acetolysis and methylation analysis of mutant mannans indicated that both N3 and N3-8 mutant mannans lacked α-1,2-mannosyl side chains which are normally present. Structures of alkali-soluble β-glucans from N3 and N3-8 mutant cells were also different from that of wild type cells in the ratio of 1,6-linkages to 1,3-linkages. The mutant glucans have much higher ratios of β-1,6-linkages (2 times for N3 mutant, 4 times for N3-8 mutant) than wild type glucan.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 3","pages":"Pages 251-255"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90101-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79881789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ki Du Nam , Meung Ho Choi , Woon Sik Kim , Hae Sung Kim , Beung Ho Ryu
{"title":"Simultaneous saccharification and alcohol fermentation of unheated starch by free, immobilized and coimmobilized systems of glucoamylase and Saccharomyces cerevisiae","authors":"Ki Du Nam , Meung Ho Choi , Woon Sik Kim , Hae Sung Kim , Beung Ho Ryu","doi":"10.1016/0385-6380(88)90010-6","DOIUrl":"10.1016/0385-6380(88)90010-6","url":null,"abstract":"<div><p>For the simultaneous saccharification and alcohol fermentation (SSF) of corn, cassava and naked barley requiring no heat treatment, the use of freely syuspended glucoamylase-cell, cell immobilized and glucoamylase-cell coimmobilized systems in Ca-alginate was investigated.</p><p>In these systems, 0.2% glucoamylase was used for both the free glucoamylase-cell and cell immobilized systems, whereas 1.0% of the enzyme was used for the glucoamylase cell coimmobilized system.</p><p>The SSF of corn, cassava and naked barley was successfully carried out as follows; group A with heat treatment and group B without heat treatment using the glucoamylase-cell suspende system. The conversion yield (<em>Y</em><sub>p/s0</sub>) in the cell immobilized system was increased by the heat treatment of corn and cassava but not of naked barley. The conversion yield was remarkably increased using the coimmobilized system corn and cassava, whereas naked barley gave the same yield as in the free glucoamylase-cell suspended system.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 4","pages":"Pages 427-432"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90010-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72912661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Production of xylitol from D-xylulose by Mycobacterium smegmatis","authors":"Ken Izumori, Keiji Tuzaki","doi":"10.1016/0385-6380(88)90126-4","DOIUrl":"10.1016/0385-6380(88)90126-4","url":null,"abstract":"<div><p><em>Mycobacterium smegmatis</em> transformed <span>D</span>-xylulose to xylitol in washed cell reactions under aerobic and anaerobic conditions. The yield of xylitol reached about 70% in anaerobic conditions (in N<sub>2</sub>) by cells grown on media containing xylitol or <span>D</span>-mannitol. Cells immobilized with Ca-alginate had almost the same activity of xylitol production as washed cells.</p><p>Xylitol was produced from <span>D</span>-xylose using commercial immobilized <span>D</span>-xylose isomerase from <em>Bacillus coagulans</em> and immobilized cells of <em>M. smegmatis</em>. From 10 g of <span>D</span>-xylose, 4 g of xylitol was produced and 5 g of <span>D</span>-xylose remained in the reaction mixture; no <span>D</span>-xylulose was detected.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 1","pages":"Pages 33-36"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90126-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86061637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}