{"title":"Analysis of a promoter-like region in Flavobacterium which controls 6-aminohexanoic acid linear oligomer hydrolase gene expression in Escherichia coli","authors":"Mitsuo Okazaki , Tomohiro Uemura , Yasumoto Nishimori , Kiichiro Wakamatsu , Makoto Shimosaka , Hideki Saito , Seiji Negoro , Hirosuke Okada","doi":"10.1016/0385-6380(88)90080-5","DOIUrl":null,"url":null,"abstract":"<div><p>The region located 6.0–6.2 kb upstream from a structural gene for 6-aminohexanoic acid linear oligomer hydrolase (EII) in <em>Flavobacterium</em> was found to be responsible for EII expression in <em>Escherichia coli</em>. In this region there existed a 50 bp long inverted repeat containing a sequence homologous to the <em>E. coli</em> promoter −35 and −10 regions. Introduction of deletions in this region resulted in decreases in EII activity to various levels.</p></div>","PeriodicalId":15702,"journal":{"name":"Journal of Fermentation Technology","volume":"66 5","pages":"Pages 489-494"},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0385-6380(88)90080-5","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0385638088900805","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
The region located 6.0–6.2 kb upstream from a structural gene for 6-aminohexanoic acid linear oligomer hydrolase (EII) in Flavobacterium was found to be responsible for EII expression in Escherichia coli. In this region there existed a 50 bp long inverted repeat containing a sequence homologous to the E. coli promoter −35 and −10 regions. Introduction of deletions in this region resulted in decreases in EII activity to various levels.
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