Journal of Dental Research最新文献

{"title":"EP2 Modulates Satellite Glial Cell Activation in Temporomandibular Joint Osteoarthritis Chronic Pain via p-ERK1/2 Signaling.","authors":"X Ma,Y Yuan,T Zhu,X Liu,R Chen,X Zhang,Z Qin,J Zhao,Y Feng,H Li,Y Liu,J Ke","doi":"10.1177/00220345251328889","DOIUrl":"https://doi.org/10.1177/00220345251328889","url":null,"abstract":"The etiology of chronic pain in temporomandibular joint osteoarthritis (TMJOA) is still unclear, making its treatment challenging in clinical practice. Emerging evidence suggests that the activation of satellite glial cells (SGCs) exerts an important role in the development of pain. This study aims to investigate whether and which prostaglandin E receptor (EP) subtypes expressed on peripheral SGCs and how the corresponding EP subtypes modulate SGC activation during TMJOA chronic pain. Immunofluorescence double staining was applied to demonstrate that EP2 and EP3 expressed on the activated SGCs in the trigeminal ganglions of mice. In vitro studies on the cultivation of primary SGCs showed that EP2 antagonist PF-04418948 significantly attenuated SGC activation in a dose-dependent manner, while EP3 agonist sulprostone failed to affect SGC activation. Kyoto Encyclopedia of Genes and Genomes analysis of RNA sequencing and Western blot demonstrated that the EP2-mediated signaling pathways were associated with phosphorylated extracellular signal-regulated kinase 1 and 2 (p-ERK1/2) signaling of mitogen-activated protein kinases (MAPKs). In addition, to verify the involvement of EP2 on SGCs in the activation of SGCs in vivo, a recombinant adeno-associated virus vector containing glial fibrillary acidic protein-shRNA (EP2)-enhanced green fluorescent protein was injected into TMJOA mouse ganglion of the third branch of the trigeminal nerve to knockdown EP2 on the SGCs. Taken together, EP2 modulates SGC activation through MAPK/p-ERK1/2 signaling in the chronic pain of monosodium iodoacetate-induced TMJOA. This study reveals a new mechanism of SGC activation, providing new insights for the treatment of chronic pain in TMJOA.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"74 1","pages":"220345251328889"},"PeriodicalIF":7.6,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preconception Oral Health Is Associated with Modifiable Health Behaviors.","authors":"J C Bond,M A Simancas-Pallares,K Divaris,R I Garcia,M P Fox,L A Wise,B Heaton","doi":"10.1177/00220345251325216","DOIUrl":"https://doi.org/10.1177/00220345251325216","url":null,"abstract":"The health of prospective parents before conception (i.e., preconception health) has important intergenerational consequences. Although oral health is associated with several reproductive outcomes, it is often absent from preconception health promotion. To generate insights that may inform preconception oral health interventions, we used cross-sectional data from 6,159 US-based participants enrolled in the Pregnancy Study Online, a prospective cohort study of pregnancy planners, to characterize preconception oral health care engagement and self-rated oral health. We used latent class analysis to identify homogenous subgroups (i.e., latent classes) of participants with respect to modifiable risk behaviors and quantified associations between class membership and 3 outcomes-reporting no dental visit within the past year, reporting no dental cleaning within the past year, and self-rated oral health-using log binomial regression models. We identified 3 distinct groups of participants: healthy behavior, high prevalence of healthy behaviors; some risk behavior, higher sugar-sweetened beverage consumption and lower vaccine uptake and multivitamin use; and most risk behavior, high probability of most risk behaviors, including current or former cigarette smoking. The some risk behavior class was more likely to report no dental visit within the past year as compared with the healthy behavior class (prevalence difference [PD] 0.17; 95% confidence interval [95% CI], 0.14 to 0.19). This association strengthened when most risk behavior was compared with healthy behavior (PD, 0.32; 95% CI, 0.28 to 0.36). Similarly, reporting no dental cleaning within the past year was more prevalent among some risk behavior (PD, 0.19; 95% CI, 0.16 to 0.21) and most risk behavior (PD, 0.38; 95% CI, 0.34 to 0.42) as compared with healthy behavior. The pattern was similar for self-rated oral health. Our findings suggest that oral health care engagement and self-rated oral health are associated with other modifiable behaviors in the preconception period. Health promotion efforts in the preconception period must consider oral health care engagement alongside other modifiable health behaviors.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"35 1","pages":"220345251325216"},"PeriodicalIF":7.6,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and Antioxidant GelMA/CeO 2 Hydrogel Promotes Oral Mucosal Healing","authors":"F. Yang, Y. He, F. Wang, X. Zhao, Y. Hsu, F. Yan, X. Zhou, W. Hu, D. Xia, Y. Liu","doi":"10.1177/00220345251323264","DOIUrl":"https://doi.org/10.1177/00220345251323264","url":null,"abstract":"Oral mucosal wounds can greatly affect overall patient health and interfere with eating and speech functions. The intraoral environment provides an ideal milieu for bacterial growth and proliferation, thereby increasing the risk of infection in oral mucosal wounds. In the event of wound infection, immune cells undergo a respiratory burst, leading to the production of a substantial amount of reactive oxygen species. Bacterial colonization and excessive reactive oxygen species can induce adverse inflammatory responses, resulting in delayed healing of oral mucosal wounds. Cerium oxide (CeO <jats:sub>2</jats:sub> ) nanoparticles were chosen for their high antibacterial and antioxidant capacities. Gelatin methacrylate (GelMA) is an excellent carrier of CeO <jats:sub>2</jats:sub> nanoparticles, which has good injectivity after precrosslinking and is suitable for deep oral wounds. In this study, GelMA/CeO <jats:sub>2</jats:sub> hydrogels with antibacterial and antioxidant functionalities were engineered to enhance the healing of oral mucosal wounds. The GelMA/CeO <jats:sub>2</jats:sub> hydrogels demonstrated excellent biocompatibility, promoted gingival fibroblast migration, and upregulated the expression of genes involved in wound healing. In addition to their significant antioxidant activity, the GelMA/CeO <jats:sub>2</jats:sub> hydrogels exhibited strong antibacterial properties, as evidenced by the inhibition of <jats:italic>Streptococcus mutans</jats:italic> and <jats:italic>Porphyromonas gingivalis</jats:italic> growth. The GelMA/CeO <jats:sub>2</jats:sub> hydrogels also significantly accelerated the healing of infected mucosal wounds in a rabbit model, achieving a 100% wound closure rate by day 7. In summary, the use of these novel GelMA/CeO <jats:sub>2</jats:sub> hydrogels represents an effective strategy for the treatment of oral wounds.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"10 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Tooth Maturity on Healing Outcomes in Regenerative Endodontics","authors":"S. Takahara, N. Ohkura, N. Yoshiba, R. Baldeon-Gutierrez, S. Gomez-Kasimoto, N. Edanami, T. Ida, S. Takenaka, K. Yoshiba, Y. Noiri","doi":"10.1177/00220345251325826","DOIUrl":"https://doi.org/10.1177/00220345251325826","url":null,"abstract":"Regenerative endodontic procedures using blood clots (BC-REP) for immature teeth typically exhibit a periodontal ligament–like healing pattern. However, a pulp-like healing pattern is observed in the presence of residual pulp. This study aimed to clarify the healing phenotype according to tooth maturity when performing BC-REP in the presence of residual pulp, focusing on migrated mesenchymal stem/stromal cells (MSCs). BC-REP rat molar models were created in the presence of residual pulp at ages corresponding to tooth developmental stages, from immature to mature (5 wk: immature, root is still growing; 8 wk: near mature, root has finished growing in length but the apex is not formed; 11 wk: mature, the apex is formed). The healing pattern and histological MSC markers (α–smooth muscle actin [α-SMA], CD73, CD90, and CD146) were investigated. The frequency of periodontal ligament–like healing was higher in mature teeth than in immature teeth. In addition, more healing macrophages were observed at the apical site 28 d after BC-REP, which is the final stage of healing. In immature teeth, double-immunopositive cells for proliferation markers (ki67 and proliferating cell nuclear antigen [PCNA]) and α-SMA were frequently observed in the vicinity of the root canal orifice 7 d after treatment, which is the early stage of healing. By contrast, in mature teeth, the number of CD73-, CD90-, and CD146-immunopositive cells increased at the apical site after 7 and 28 d. CD90- and CD146-immunopositive cells expressed cell proliferation markers (ki67 or PCNA) after 7 d. MSC migration after BC-REP likely varies based on tooth maturity, resulting in different healing phenotypes.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"57 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Don’t Keep Calm, Give a Damn!","authors":"E. Ioannidou","doi":"10.1177/00220345251325237","DOIUrl":"https://doi.org/10.1177/00220345251325237","url":null,"abstract":"","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"60 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143832026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and Transcriptomic Adaptation to Chlorhexidine in Streptococcus spp.","authors":"B. Daller, D.L. Auer, W. Buchalla, S. Bartsch, A. Gessner, N.S. Jakubovics, A. Al-Ahmad, A. Hiergeist, F. Cieplik","doi":"10.1177/00220345251320912","DOIUrl":"https://doi.org/10.1177/00220345251320912","url":null,"abstract":"Antiseptics such as chlorhexidine digluconate (CHX) are widely used in clinical dental practice, but their potential risks, particularly regarding antimicrobial resistance (AMR), are not yet known. This study explores the genomic and transcriptomic mechanisms of CHX adaptation in 3 clinical isolates of <jats:italic>Streptococcus</jats:italic> spp. and their adapted counterparts. The genomic analysis revealed mutations in genes related to membrane structure, DNA repair, and metabolic processes. Mutations include those in diacylglycerol kinase that occurred in <jats:italic>Streptococcus salivarius</jats:italic> and the autolysin N-acetylmuramoyl-L-alanine amidase homologues in both <jats:italic>Streptococcus mitis</jats:italic> and <jats:italic>Streptococcus vestibularis</jats:italic> , which may contribute to enhanced CHX resistance. Our findings showed stress response genes constantly expressed in all 3 CHX-adapted strains, regardless of acute CHX exposure. Commonly upregulated genes were related to oxidative stress, DNA repair, and metabolic pathway changes, especially amino acid related metabolism. In addition, cell surface restructuring, multiple ABC transporter genes, as well as antimicrobial resistance–associated genes were constitutively expressed. Homologue genes that were significantly upregulated across all 3 species after mutation included <jats:italic>recD</jats:italic> (DNA repair), <jats:italic>potE</jats:italic> (amino acid transport), and <jats:italic>groEL</jats:italic> (stress response). In addition, we saw an increase in a gene associated with the penicillin-binding protein PBP2a in all strains. Beyond these conserved adaptations, we observed species-specific shifts under prolonged CHX exposure. In <jats:italic>S. vestibularis</jats:italic> , glutathione synthesis genes increased while fatty acid metabolism genes were downregulated. <jats:italic>S. salivarius</jats:italic> showed elevated expression of genes related to organic anion transport and RNA modification. <jats:italic>S. mitis</jats:italic> exhibited changes in pyrimidine metabolism, ion homeostasis, and pyruvate dehydrogenase complex genes. Uniquely, <jats:italic>S. mitis</jats:italic> also showed acute CHX response with upregulation of carbohydrate metabolism and phosphotransferase system genes. These findings highlight the complexity of CHX-induced adaptation, suggesting connections to genetic mutations and emphasizing the need for further research to understand and mitigate AMR risks.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"16 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNMTi@ZIF-8 Enhances Biomimetic Pulp Regeneration via Epigenetic Regulation","authors":"Z. Li, M. Wan, D. Cui, Q. Tian, Y. Li, S. Yu, L. Zheng, L. Ye","doi":"10.1177/00220345251315468","DOIUrl":"https://doi.org/10.1177/00220345251315468","url":null,"abstract":"Regenerating the functional dentin–pulp complex remains a significant challenge in endodontics. Conventional regenerative endodontic therapies often result in the formation of non–pulp-like tissue due to the uncontrolled induction of stem cells and cytokines. Mimicking developmental processes to promote regeneration represents a promising yet challenging approach in regenerative medicine. This study aimed to develop a biomimetic regenerative therapy that integrates a DNMTi@ZIF-8 nanoplatform with dental pulp stem cell (DPSC) spheroids to effectively regenerate the dentin–pulp complex. First, a progressive reduction in 5-methylcytosine content was revealed to be a core signal in the odontogenic differentiation process. Based on this discovery, DNA methyltransferase inhibitors (DNMTi) were further used to simulate this regulatory process. The results showed that DNMTi not only significantly promoted odontogenic differentiation but also inhibited the angiogenesis process. To address this dual effect, in situ synthesized zeolitic imidazolate framework-8 (ZIF-8) was used for the delivery of DNMTi. This DNMTi@ZIF-8 system not only prolonged drug activity but also enhanced angiogenesis-promoting efficacy by activating the PI3K-AKT signaling pathway through the sustained release of zinc ions, assessed via angiogenic assays including scratch assays, tube formation assay, and chick chorioallantoic membrane assay. When integrated with DPSC spheroids engineered via agarose microwells, analyzed through odontogenic differentiation assays, this system demonstrated significantly enhanced odontogenic differentiation capabilities. Moreover, the introduced biomimetic regenerative therapy successfully regenerated the dentin–pulp complex in a semi-orthotopic in vivo model. This biomimetic developmental approach not only addresses critical gaps in dental tissue engineering but also highlights a new direction for treating pulp and periapical diseases, underscoring its broader implications in regenerative medicine.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"57 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to MMP-8-Responsive Polyethylene Glycol Hydrogel for Intraoral Drug Delivery","authors":"","doi":"10.1177/00220345251330904","DOIUrl":"https://doi.org/10.1177/00220345251330904","url":null,"abstract":"","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"57 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating Periodontal Stability Using Computer Vision","authors":"B. Feher, A.A. Werdich, C.-Y. Chen, J. Barrow, S.J. Lee, N. Palmer, M. Feres","doi":"10.1177/00220345251316514","DOIUrl":"https://doi.org/10.1177/00220345251316514","url":null,"abstract":"Periodontitis is a severe infection affecting oral and systemic health and is traditionally diagnosed through clinical probing—a process that is time-consuming, uncomfortable for patients, and subject to variability based on the operator’s skill. We hypothesized that computer vision can be used to estimate periodontal stability from radiographs alone. At the tooth level, we used intraoral radiographs to detect and categorize individual teeth according to their periodontal stability and corresponding treatment needs: healthy (prevention), stable (maintenance), and unstable (active treatment). At the patient level, we assessed full-mouth series and classified patients as stable or unstable by the presence of at least 1 unstable tooth. Our 3-way tooth classification model achieved an area under the receiver operating characteristic curve of 0.71 for healthy teeth, 0.56 for stable, and 0.67 for unstable. The model achieved an F <jats:sub>1</jats:sub> score of 0.45 for healthy teeth, 0.57 for stable, and 0.54 for unstable (recall, 0.70). Saliency maps generated by gradient-weighted class activation mapping primarily showed highly activated areas corresponding to clinically probed regions around teeth. Our binary patient classifier achieved an area under the receiver operating characteristic curve of 0.68 and an F <jats:sub>1</jats:sub> score of 0.74 (recall, 0.70). Taken together, our results suggest that it is feasible to estimate periodontal stability, which traditionally requires clinical and radiographic examination, from radiographic signal alone using computer vision. Variations in model performance across different classes at the tooth level indicate the necessity of further refinement.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"61 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Global Consortium of Oral Health Birth Cohort Studies—GLOBICS","authors":"K.G. Peres, G.G. Nascimento, S. Sarawagi, K. Gambetta-Tessini, A.C. Kalhan, H. Li, C.A. Feldens, B.W. Chaffee, A.J.D. Barros, A. Rugg-Gunn, M.A. Peres, A.N. Åstrøm, J. Abanto, N. Birungi, M.A. Cardoso, M.A. Charles, M.B. Correa, S. Dashper, F.F. Demarco, A. Germa, P.C. Hallal, C. Jacobs, L. Kragt, P.F. Kramer, S. Levy, T.A. Marshall, A. Nirunsittirat, W. Pitiphat, C.C.C. Ribeiro, C. Sieng, E.B.A.F. Thomaz, B. Turton, M.R. Vitolo, Y. Wagner","doi":"10.1177/00220345251315700","DOIUrl":"https://doi.org/10.1177/00220345251315700","url":null,"abstract":"This article highlights the transformative initiatives of the Global Consortium of Oral Health Birth Cohort Studies (GLOBICS) as a key response to the call for a global overhaul of the oral health agenda. Recognizing the critical role of birth cohort studies in public health, particularly in understanding the interplay between oral health, noncommunicable diseases (NCDs), and social inequalities, GLOBICS is spearheading efforts that are research driven and focused on translational pathways. GLOBICS is advancing an International Research Agenda with 8 key priorities aimed at addressing the most pressing questions in oral health research. These priorities shall guide pooled analyses from oral health birth cohort studies (OHBCS) spread across the globe, enabling the consortium to generate robust evidence answering core research questions. One of the major efforts of GLOBICS is data harmonization, which involves standardizing and integrating data from multiple sources to ensure consistency and comparability. This process is vital for generating reliable global insights and fostering collaborations with new research partners. GLOBICS also emphasizes the nurturing of the next generation of OHBCS researchers, ensuring that the field continues to grow and innovate. The consortium’s commitment to disseminating and translating its findings is central to its mission. By supporting the development of clinical practice guidelines, policies, and public health information tailored to the specific contexts of its collaborators, GLOBICS is making strides toward tangible improvements in global oral health. This work is a testament to the power of collaborative research and an invitation for further contributions to this global effort.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"183 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}