Journal of Dental Research最新文献

{"title":"The Submucosal Microbiome Correlates with Peri-implantitis Severity","authors":"A.A. Joshi, S.P. Szafrański, M. Steglich, I. Yang, W. Behrens, P. Schaefer-Dreyer, J. Grischke, S. Häussler, M. Stiesch","doi":"10.1177/00220345251352809","DOIUrl":"https://doi.org/10.1177/00220345251352809","url":null,"abstract":"Peri-implantitis is an increasingly prevalent chronic inflammatory disease of the peri-implant tissue. A key etiologic factor for peri-implantitis is the submucosal biofilm, which may further drive clinical severity and accelerate disease progression. The present cross-sectional study aimed to characterize the compositional (full-length 16S rRNA gene amplicon sequencing) and functional patterns (metatranscriptomics) in the microbiome as an indicator of peri-implantitis severity. For this purpose, submucosal biofilm samples were collected from 49 peri-implantitis–diagnosed implants in 34 patients. Notable microbial signatures were associated with increased probing depth (PD), a measure for disease severity. Multivariate linear regression analysis, adjusted for patient variability, showed that the genera <jats:italic>Capnocytophaga</jats:italic> and <jats:italic>Gemella</jats:italic> were negatively correlated with increased peri-implantitis severity whereas <jats:italic>Pseudoramibacter</jats:italic> was positively correlated with it. PICRUSt2-based metabolic pathway prediction revealed the following to be negatively correlated with PD: central carbon metabolism, nitrate reduction, sulfate assimilation, glycolysis, the tricarboxylic acid cycle, and heme biosynthesis. In contrast, cobalamin and tetrahydrofolate biosynthesis showed positive correlations. Metatranscriptomic analysis uncovered additional enzyme functions correlated with PD, which were related to galactose metabolism and proteolysis. Our data allowed the proposal of an extended microbial dysbiosis index for peri-implantitis severity. The quantitative index, integrating significant microbial and functional features, revealed high correlation with PD. In conclusion, our results showed that the level of peri-implantitis severity is associated with distinct and significant changes in microbial composition as well as microbial functions. The severity-specific microbiome signatures identified in our study will advance microbiome-based diagnostics and disease stratification, paving the way for targeted clinical interventions for peri-implantitis.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"125 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144719396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced Laboratory Assessments in Caries Mineralization Research: A Review","authors":"Y. Fu, F. Nudelman, Y. Zhang, K.C. Li, M. Ekambaram, P.R. Cooper, M.L. Mei","doi":"10.1177/00220345251356472","DOIUrl":"https://doi.org/10.1177/00220345251356472","url":null,"abstract":"Dental caries is characterized by the dynamic processes of demineralization and remineralization. Recent advancements in cariology research have introduced sophisticated techniques such as nanoindentation, nano-computed tomography, optical coherence tomography, X-ray photoelectron spectroscopy, atom probe tomography, and transmission electron microscopy, which provide high resolution and sensitive analytics. These methods are particularly valuable for detecting micro- and nano-scale changes in individual lesions, crucial for short-term in vitro studies. They enable assessments of mechanical properties, mineral density, chemical composition, and morphological structure, providing insights into the dynamic nature of caries and aiding in the optimization of caries management strategies and the development of new treatments. Results from these techniques have been validated through in vivo studies by correlating noninvasive imaging with clinical caries progression and monitoring mineral density changes over time. A comprehensive understanding of these techniques is essential to prevent misselection, inappropriate sample preparation, and data misinterpretation. This review focuses on methods applicable to the analysis of early caries lesions based on recent literature and provides detailed insight into a variety of relevant techniques. In addition, it discusses parameters such as main outcome measurements, resolution/magnification, invasiveness, and sample preparation requirements of each approach, thereby providing valuable guidance for researchers planning studies in this field.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"26 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Absence of Claudin-10 in the Enamel Organ Alters Its Integrity","authors":"T.N. Nguyen, S. Ribes, C. Andrique, M. Requin, J. Bouchet, N. Obtel, L. Slimani, N. Brouilly, C. Torrens, A. Schmitt, T. Guilbert, M. Morawietz, A. Kiesow, A. Brunelle, A. Percot, S. Hadj-Rabia, C. Gaucher, A. Le Bivic, P. Houillier, C. Bardet, D. Muller, F. Ramirez Rozzi, T. Coradin, T. Breiderhoff, C. Chaussain","doi":"10.1177/00220345251349109","DOIUrl":"https://doi.org/10.1177/00220345251349109","url":null,"abstract":"Rare disorders related to tight junction (TJ) proteins have been associated with amelogenesis imperfecta. Pathogenic variants of <jats:italic>CLDN10</jats:italic> , encoding claudin-10b, a cation transport pore, cause the autosomal recessive HELIX syndrome (Hypohidrosis, Electrolyte imbalance, hypoLacrymia, Ichthyosis, Xerostomia). Patients exhibit salivary dysfunction and rapid enamel wear after tooth eruption. Since C <jats:italic>ldn10</jats:italic> is expressed in the dental epithelium, this study explores the role of claudin-10b in amelogenesis. We analyzed amelogenesis in constitutive and conditional <jats:italic>Cldn10</jats:italic> knockout (KO) murine models, comparing the findings to human HELIX enamel. First, analysis of constitutive <jats:italic>Cldn10</jats:italic> knockout (KO) mice, which die within a few hours after birth, showed that claudin-10 is present at the plasma membrane of the stratum intermedium but not at the TJs during the secretory stage. Its absence altered gene expression related to ion transport and pH control, although without major disturbance in cell polarization or enamel matrix synthesis. Examination of later stages of amelogenesis in epithelium-targeted conditional <jats:italic>Cldn10</jats:italic> KO mice showed that claudin-10 is present in the papillary layer at the maturation stage. In its absence, the pH of the enamel matrix was more basic during early maturation, suggesting that claudin-10 determines enamel matrix pH. However, at later stage of the maturation process, the pH was corrected and the resulting enamel did not show major structural or elemental alterations. These later findings were confirmed by exploring the enamel of <jats:italic>Cldn10</jats:italic> KO transplanted tooth germs, which have developed in a controlled mineral environment. Nevertheless, higher contents of aluminum were detected in the enamel of transplanted germs and in human HELIX enamel, suggesting that claudin-10 deficiency may lead to a loss of enamel organ integrity. These data suggest that while salivary dysfunction is the main cause of enamel wear in HELIX, claudin-10 plays a direct role in amelogenesis by determining pH and enamel organ integrity.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"97 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lanthanum Ion–Engineered Hybrid Layer Improves Dentin Bonding","authors":"X. Li, Y. Wu, Y. Lu, C. Shu, Y. Chen, Z. Zhang, X. Li, Q. Luo","doi":"10.1177/00220345251353388","DOIUrl":"https://doi.org/10.1177/00220345251353388","url":null,"abstract":"The retreatment of failed adhesive restorations consumes more than 60% of clinical resources. Patient factors—such as caries risk, oral hygiene, diet, and occlusal stress—contribute to failure primarily. However, the contribution of the degradation of the adhesive–dentin interface to the failure cannot be ignored and involves 3 interrelated challenges: (1) highly hydrated demineralized dentin matrix (DDM) hindering adhesive infiltration, particularly in the partially demineralized zone; (2) residual hydroxyapatite (HAP) prone to dissolution; and (3) degradation of unprotected collagen by acid-activated matrix metalloproteinases (MMPs) and colonized microbes. To synchronously address all 3 failure mechanisms, a breakthrough strategy was developed based on lanthanum ion (La <jats:sup>3+</jats:sup> ) pretreatment. Morphological changes were observed by field emission scanning electron microscopy and transmission electron microscopy; chemical and crystal properties were analyzed by selected area electron diffraction, energy-dispersive X-ray spectroscopy, X-ray diffractometry, and X-ray photoelectron spectroscopy; microhardness and surface potential were detected by atomic force microscopy; water content and type were characterized by attenuated total reflection–Fourier transform-infrared spectroscopy; enzyme inhibition was assayed by in situ zymography; adhesive infiltration was traced by Nile red; bonding effectiveness was mainly measured through microtensile bonding strength test and nanoleakage; and antibacterial activity testing was carried out on <jats:italic>Streptococcus mutans</jats:italic> . After a 20-s treatment of the DDM with a drop of LaCl <jats:sub>3</jats:sub> solution before applying the adhesive, (1) noncollagenous protein aggregation induced DDM dehydration, in turn enhancing adhesive infiltration; (2) MMP was inhibited (almost 100% activity reduction) and <jats:italic>S. mutans</jats:italic> was suppressed; and (3) atomic-scale HAP remodeling was performed via Ca <jats:sub>8</jats:sub> La <jats:sub>2</jats:sub> (PO <jats:sub>4</jats:sub> ) <jats:sub>6</jats:sub> O <jats:sub>2</jats:sub> crystallization, increasing thermodynamic stability. This trimodal intervention creates a defect-minimized hybrid layer with enzymatic resistance, bacteria inhibition, and structurally reinforced base, demonstrating 77.30% higher bonding strength after 10,000 thermocycles versus controls. The protocol’s chairside compatibility (20 s of chair time) and biosafety validation establish La <jats:sup>3+</jats:sup> -assisted bonding as a clinically translatable strategy to disrupt the restoration failure cycle, offering transformative potential for sustainable dental care.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"284 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144719519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term Impact of Kindergarten Dental Outreach Service on School Students.","authors":"S Wu,E C M Lo,C H Chu,M C M Wong","doi":"10.1177/00220345251347943","DOIUrl":"https://doi.org/10.1177/00220345251347943","url":null,"abstract":"This retrospective observational study investigated the association between kindergarten dental outreach service provided by the Faculty of Dentistry, University of Hong Kong, and dental caries experience and treatment received among primary school students using electronic data retrieved from the School Dental Care Service (SDCS) provided by the Hong Kong government. The outreach service encompassed oral examination, oral health education, and silver diamine fluoride application. Grade 3 or 4 primary school students participating in the SDCS were recruited from school dental clinics in 2022. Questionnaire surveys were administered to children and their parents. Children's clinical records since primary school entry were obtained from the SDCS. Children were classified into the exposure group if they attended kindergartens that received outreach services from 2015 to 2019; others were classified as the nonexposure group. The inverse probability weighting method was used to adjust for confounding. Among 964 children with clinical data, 180 (18.7%) were in the exposure group, and 784 (81.3%) were in the nonexposure group. From grades 1 to 3, the respective prevalences of caries experience were 54.6%, 60.7%, and 57.6% and the mean (SD) dmft scores were 2.5 (3.1), 2.4 (2.8), and 2.0 (2.4). After adjustment, the exposure group showed a lower prevalence of decayed primary teeth in grade 3 (difference, -9.8%; 95% CI, -18.0% to -1.6%; P = 0.019) and a lower mean number of decayed primary teeth in grade 2 (difference, -0.4; 95% CI, -0.7 to -0.1; P = 0.017). Children received a mean (SD) 6.6 (5.7) treatment items over 3 school years, with annual means of 2.7 (3.3), 2.0 (3.0), and 1.9 (2.2) from grades 1 to 3. No significant association was found between exposure and treatment received. In conclusion, the outreach service demonstrates a promising long-term impact in preventing caries in primary teeth among primary school children.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"10 1","pages":"220345251347943"},"PeriodicalIF":7.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144652706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Aging Oral Health on Long COVID-19.","authors":"P A Abdul-Azees,M Marinkovic,B B Singh,D D Dean,X-D Chen,M P Goldberg,M I Restrepo,P M Loomer,C-K Yeh","doi":"10.1177/00220345251349805","DOIUrl":"https://doi.org/10.1177/00220345251349805","url":null,"abstract":"At least 10% of individuals infected with SARS-CoV-2 develop a variety of multisystem symptoms lasting more than 12 wk known as postacute sequelae of COVID-19 (PASC) or \"long COVID.\" While the precise pathogenesis of PASC remains unclear, immune dysregulation is widely recognized as a key factor. Moreover, PASC disproportionately affects older individuals who are prone to age-related immune system decline, which further exacerbates the risk and severity of PASC. The oral cavity, a primary site for initial SARS-CoV-2 infection, may contribute to the development and persistence of PASC. Emerging evidence suggests that changes in age-related oral health, such as periodontitis, salivary gland (SG) dysfunction, and gustatory and olfactory impairments, may create an environment conducive to forming an oral reservoir of intact virus or viral antigens, which may contribute to the chronicity of PASC. Alternatively, the pathogenesis of PASC may increase the risk of a wide range of oral health issues, such as dental diseases, dry mouth, and sensory dysfunction (e.g., taste and smell) that are frequently reported by patients with PASC. This review highlights how aging facilitates oral SARS-CoV-2 infection, co-infection with other viruses may drive PASC in aging patients, aging and PASC dysregulate the oral microbiome, SARS-CoV-2 infection promotes molecular mechanisms involved in oral tissue aging, aging oral health affects susceptibility to developing PASC, and inflammation associated with PASC exacerbates dysregulation of metabolic/enzymatic pathways of aging oral mucosa and diseases of the periodontal apparatus, SGs, and taste. It underscores the urgent need to comprehensively address the interplay between aging oral health and PASC, which will help mitigate long-term complications and improve overall health outcomes for affected individuals.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"27 1","pages":"220345251349805"},"PeriodicalIF":7.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144652739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Bio-heterojunctions Targeting Biofilm for Caries Treatment.","authors":"J Luo,M Li,Y Luo,Y Deng,J Li,K Liang","doi":"10.1177/00220345251347918","DOIUrl":"https://doi.org/10.1177/00220345251347918","url":null,"abstract":"Phototherapy has become pivotal in the management of dental caries, recognized for its controllable, nonresistant, and antibacterial properties. However, photoresponsive materials applied in fluid-filled oral cavities present challenges-specifically, retaining them within cariogenic biofilms is difficult, and when such agents are carried away, it diminishes the effectiveness of phototherapy. . Additionally, the lack of targeting efficiency for cariogenic biofilms and on-demand release mechanisms results in decreased bioavailability, leading to unsatisfactory antibacterial efficacy and potential side effects. To address the dilemma, a hyaluronic acid (HA)-based MXene/CuS (MCH) bio-heterojunction is elaborated and activated by the cariogenic biofilm microenvironment to exert efficient sterilization and biofilm elimination effects for rescuing dental caries. In this intelligent system, HA initiates accurate anchors by targeting hyaluronidase (HAase) derived from the cariogenic biofilm microenvironment, and the encapsulated MXene/CuS (MC) bio-heterojunction is released on demand as the HAase-triggered reaction intensifies within the biofilms. Under near-infrared (NIR) irradiation, the liberated MC is endowed with exceptional photosensitive attributes, simultaneously generating thermal energy and reactive oxygen species to perform synergistic photothermal and photodynamic therapy. In vitro results display that in cariogenic biofilms, the HA layer of MCH can react with HAase and undergo degradation, while maintaining a sufficient on-demand release of MC even under fluid rinsing. Subsequently, MCH and NIR irradiation combat bacteria and biofilm infection effectively through phototherapy physical mechanisms. Also, in vitro results demonstrate that oral cells perform normal extended morphology and considerable proliferation trends after MCH treatment. In addition, in vivo experiments authenticate that the MCH + NIR group shows excellent antibacterial efficacy, and micro-computed tomography analysis and the modified Keyes score calculation indicate more intact enamel with no caries observed on rat molars. This pioneering approach heralds a promising avenue for the application of cariogenic biofilm microenvironment-activated photoresponsive materials in the domain of dental caries prevention and treatment.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"10 1","pages":"220345251347918"},"PeriodicalIF":7.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144652887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Science System at the Limit: A Call to Action for Considerate Publishing.","authors":"F Schwendicke,S E Uribe,N S Jakubovics","doi":"10.1177/00220345251349804","DOIUrl":"https://doi.org/10.1177/00220345251349804","url":null,"abstract":"In recent years, the landscape of scientific publishing has experienced exponential growth, driven in part by the increasing availability of data and advanced analytical methods, by incentives set by the scientific career system and by increasing options of publication routes, with diverging rigor in peer review. While this surge offers unprecedented opportunities for exploration and innovation, it also introduces challenges that potentially compromise the quality and accessibility of scientific literature, as the number of published articles significantly outpaces the number of scientists and, hence, available peer reviewers. Moreover, the increasing complexity of scientific outputs challenges the options for comprehensive, in-depth review and reproduction. We here examine the drivers of this phenomenon, its implications for the dental research community, and potential solutions to ensure a sustainable and rigorous publishing ecosystem. Emphasizing the importance of quality over quantity, we advocate for systemic changes in academic incentives, promoting open science, and enforcing robust peer-review standards. We further summarize the recent statement of the International Committee of Medical Journal Editors toward predatory journals; the Journal of Dental Research actively endorses this statement.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"677 1","pages":"220345251349804"},"PeriodicalIF":7.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144652708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Enzymes on Dental Plaque: A Randomized Controlled Trial.","authors":"P D Rikvold,K K Johnsen,Y C Del Rey,L B S Hansen,I Knap,C Holz,R L Meyer,M R Jørgensen,S Schlafer","doi":"10.1177/00220345251347959","DOIUrl":"https://doi.org/10.1177/00220345251347959","url":null,"abstract":"The extracellular matrix has been identified as an essential determinant of virulence of dental biofilms. Therefore, enzymes that degrade the matrix are a promising preventive approach for biofilm control. Laboratory studies have consistently reported significant effects of enzyme treatment on biofilm formation, but information from randomized clinical trials (RCTs) is limited. The present triple-blind RCT investigated the effect of lozenges containing 2 different concentrations of the matrix-degrading enzymes mutanase, beta-glucanase, and DNase on de novo plaque formation, gingivitis development, and the plaque microbiome in healthy adults. Eighty subjects were enrolled with random allocation to either placebo, active 1, or active 2 (3-fold enzyme concentration). All subjects completed the study without major protocol deviations. Plaque formation was assessed after 1, 7, and 14 d of intervention without self-performed oral hygiene, using the Turesky modification of the Quigley and Hein plaque index (TM-QHPI) and the planimetric plaque index and the thickness index. Gingival index (GI) scores were registered on day 14. Moreover, the microbial plaque composition was analyzed by 16S rRNA gene sequencing, and the amount of autofluorescent plaque and plaque removal with an Airfloss device was quantified. Plaque formation was significantly lower for day 7, active 1 compared with placebo and for the pooled active groups (post hoc analysis) as well as for the pooled recordings across all time points (post hoc analysis) and for autofluorescent plaque on day 7, active 2. No significant differences in plaque formation were observed for the other time points, GI scores, or plaque removal. A nonsignificant trend toward reduced species richness was found in both active groups compared with placebo. In conclusion, multienzyme treatment may promote oral health by slightly delaying plaque formation and maturation and might serve as a supplement to mechanical plaque removal. The study was registered at Clinicaltrials.gov (NCT05082103).","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"15 1","pages":"220345251347959"},"PeriodicalIF":7.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144652738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Microbial Mosaic and Inflammatory Core of Peri-implantitis.","authors":"R C V Casarin,G M Bonilha,H Paz,T B Cerquera,C S Stolf,S P Pimentel,W A M Fernandes,M F Noronha,L M Carvalho,M Z Casati,L M Shaddox,M P Santamaria,M F Monteiro","doi":"10.1177/00220345251344585","DOIUrl":"https://doi.org/10.1177/00220345251344585","url":null,"abstract":"This study investigated the distinct microbiological and immunologic characteristics of peri-implantitis as compared with periodontitis. Both diseases are biofilm-driven inflammatory conditions affecting supportive tissues around teeth or implants, but their specific causes remain not fully understood. A cross-sectional study was carried out to compare the microbial profiles (i.e., composition and functional activity), the inflammatory mediators in crevicular fluid, and the potential impact of titanium particles in peri-implantitis. Twenty patients with moderate to severe periodontitis and 20 with peri-implantitis were included. Clinical assessments were performed, and biofilms were collected for 16S rRNA gene sequencing and metatranscriptomic analysis. Gingival crevicular fluid and peri-implant crevicular fluid were obtained to measure inflammatory markers and titanium levels (peri-implantitis group only). Results showed significant differences in microbial diversity between peri-implantitis and periodontitis. Specifically, peri-implantitis displayed enrichment of species such as Streptococcus parasanguinis, Streptococcus mutans, Cutibacterium acnes, and Aerococcus viridans. Additionally, the immune response in peri-implantitis appeared skewed toward a Th2/Th17 dominance. Analysis of the biofilm's functional activity presented heightened expression of genes associated with flagella, bacterial chemotaxis, and various metabolic pathways in the peri-implantitis group. Interestingly, titanium levels modulate the overall microbial community structure, are associated with the abundance of certain peri-implantitis-associated species, and are positively correlated with Th17 levels in PICF. Peri-implantitis harbors distinct microbial communities and inflammatory patterns, potentially influenced by titanium particles. These findings suggest a unique environment driving tissue destruction in peri-implantitis as compared with periodontitis.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"73 1","pages":"220345251344585"},"PeriodicalIF":7.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144652730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}