Journal of Dental Research最新文献

{"title":"Enamel Gene Products during Murine Amelogenesis in vivo and in vitro","authors":"H.C. Slavkin, M. Zeichner-David, M. MacDougall, C. Bessem, P. Bringas, L.S. Honig, J. Lussky, J. Vides","doi":"10.1177/0022034582061special01","DOIUrl":"https://doi.org/10.1177/0022034582061special01","url":null,"abstract":"<jats:italic toggle=\"yes\">Epithelial-mesenchymal interactions regulate determination and differentiation of amelogenesis. Our attention has focused on identification of ameloblast gene products, the regulation of enamel mRNA synthesis, and subsequent translation into enamel proteins</jats:italic> in vivo <jats:italic toggle=\"yes\">and</jats:italic> in vitro. <jats:italic toggle=\"yes\">Enamel proteins are the most abundant gene products synthesized in fully-differentiated ameloblasts. Our experimental strategy has been to isolate major proteins, produce antibodies, localize enamel protein antigens during tooth development</jats:italic> in vivo <jats:italic toggle=\"yes\">as well as</jats:italic> in vitro ( <jats:italic toggle=\"yes\">using serumless, chemically-defined medium), develop an immunoprecipitation assay, isolate poly(A)-RNA's which could be translated into specific enamel protein gene products in a cell-free translation system, and then initiate molecular cloning of the corresponding murine enamel gene(s). The major murine enamel mRNA appears to code for a predominant polypeptide of approximately 20,000 MW. Inner-enamel epithelial cells differentiate into ameloblasts, and synthesize and secrete enamel proteins within six d when cap-stage molar tooth organs are cultured in serumless, chemically-defined medium. The regulation of epithelial differentiation under these experimental conditions indicates that epithelial-mesenchymal interactions determine and maintain ameloblast differentiation</jats:italic> in vitro.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"167 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149083821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Visualization of the Patterns of Ameloblast Modulation by the Glyoxal Bis(2-hydroxyanil) Staining Method","authors":"Y. Takano, M.A. Crenshaw, J.W. Bawden, L. HammarstrÖm, S. Lindskog","doi":"10.1177/0022034582061special16","DOIUrl":"https://doi.org/10.1177/0022034582061special16","url":null,"abstract":"<jats:italic toggle=\"yes\">Several red stripes appeared on the surface of the rat incisor enamel at the maturation stage when stained with a modified glyoxal bis (2-hydroxyanil) method (GBHA). The stripes running across the maturation enamel were correlated directly, morphologically, and indirectly, using ¹⁵Ca autoradiography, with the bands of overlying smooth-ended ameloblasts. In addition, GBHA stain revealed similar stripes on the maturation enamel of the developing teeth from a variety of animals (rat molar, bovine incisor and molar, porcine canine, and monkey incisor), indicating that the maturation of the mammalian tooth enamel, in general, is under the control of two types of maturation ameloblasts which undergo cyclic morphologic changes.</jats:italic>","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"272 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149083807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Session II—Matrix-Mineralization Relationships","authors":"H. J. HÖhling, E. R. Krefting, R. Barckhaus","doi":"10.1177/0022034582061special06","DOIUrl":"https://doi.org/10.1177/0022034582061special06","url":null,"abstract":"","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"98 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149083813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of Rat Enamel Matrix Components in vivo","authors":"S. Sasaki, H. Shimokawa, K. Tanaka","doi":"10.1177/0022034582061special03","DOIUrl":"https://doi.org/10.1177/0022034582061special03","url":null,"abstract":"<jats:italic toggle=\"yes\"> The biosynthesis of enamel matrix components of developing rat incisors was in investigated by measuring the incorporation of <jats:sup>3</jats:sup> H-proline, <jats:sup>32</jats:sup> P-phosphate, and <jats:sup>35</jats:sup> S-sulfate in vivo. <jats:sup>3</jats:sup> H- and <jats:sup>32</jats:sup> P-radioactivity was found in what seemed to be a prototype of enamel proteins. Subsequent shifts in other protein and peptide fractions were observed, <jats:sup>35</jats:sup> S was also incorporated into components other than the above-mentioned proteins. </jats:italic>","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"11 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149083819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ion Transport through the Enamel Organ-An Update","authors":"J.W. Bawden, M.A. Crenshaw, Y. Takano, L. Hammarström","doi":"10.1177/0022034582061special12","DOIUrl":"https://doi.org/10.1177/0022034582061special12","url":null,"abstract":"<jats:italic toggle=\"yes\">A review of published studies of uptake of various ions in developing enamel in vivo, and related investigations conducted in vitro on the role of the enamel organ in such uptake, suggest that the environment of the forming enamel is tightly controlled by the enamel organ. A new hypothesis is proposed concerning fluoride uptake during enamel formation.</jats:italic>","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"3 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149083810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine Structure of Extracted Rat Incisor Enamel","authors":"E. Kallenbach","doi":"10.1177/0022034582061special08","DOIUrl":"https://doi.org/10.1177/0022034582061special08","url":null,"abstract":"<jats:italic toggle=\"yes\">Pieces of fresh rat incisor enamel were extracted with various buffer solutions and prepared for electron microscopy. I observed partial or complete removal of organic constituents, shrinkage of rods and interrods, separation of stippled material from the enamel, and partial decalcification. The results modify the conclusions of earlier biochemical work and point out the need for a closer correlation of biochemical and morphological approaches.</jats:italic>","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"151 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149083816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mode of Enamel Matrix Secretion","authors":"J.W. Simmelink","doi":"10.1177/0022034582061special04","DOIUrl":"https://doi.org/10.1177/0022034582061special04","url":null,"abstract":"<jats:italic toggle=\"yes\">Evidence for direct fusion of ameloblast secretary granules to the plasma membrane at the enamel forming front is minimal. Typically, secretion granules cluster in the center of the Tomes' process and frequently show continuity with a tubular reticulum containing a similar material. Secretion granules seem to exit primarily by way of plasma membrane invaginations connected to the secretory granule</jats:italic> via <jats:italic toggle=\"yes\">tubular pseudopodia. Enamel formed initially at the dentinoenamel junction, as well as interrod enamel, is also characterized by enamel matrix release into deep plasma membrane invaginations. This modified type of merocrine secretory process may play a role in enamel crystal orientation.</jats:italic>","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"481 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149083817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrinsic Tension Drives Palatal Shelf Reorientation post Tongue Retraction","authors":"C.C. Chen, S. Zhang, J.D. Morgan, C. Liu, M.A. Nye, N. Nguyen, I. Mangoba, M.M. Blanco, D.J. Volzer, C.P. Chen, D.L. Cuylear, A.H. Jheon, M.T. Cobourne, J.O. Bush, J.B.A. Green, A.F. Goodwin","doi":"10.1177/00220345261448467","DOIUrl":"https://doi.org/10.1177/00220345261448467","url":null,"abstract":"Cleft palate can be caused by failure of elevation of the palatal shelves over the tongue during development. The physical mechanism of elevation is poorly understood. It is thought that elevation is obstructed by the tongue in a condition known as Pierre Robin sequence (PRS), in which the mandible is smaller (micrognathia) and/or further back than normal (retrognathia). Some PRS cases are associated with mutations in the regulatory and coding regions of <jats:italic toggle=\"yes\">SOX9</jats:italic> . Here, we first examined the timing of palatal shelf elevation in mouse embryonic explants. While elevation in vivo occurs between embryonic days E13.5 and E14.5, we found that palate elevation occurred in both E12.5 and E13.5 explants immediately upon dissection, indicating release of elastic prestress by removal of the tongue. These findings suggest that palatal shelves are programmed ab initio to grow horizontally (as they do in birds and most reptiles) but are deflected in mammals by the tongue. Next, to examine this process in utero, we generated a new mt <jats:italic toggle=\"yes\">Hand2</jats:italic> <jats:sup>Cre</jats:sup> mouse line to delete <jats:italic toggle=\"yes\">Sox9</jats:italic> or to express diphtheria toxin specifically in the mandibular mesenchyme. <jats:italic toggle=\"yes\">Sox9</jats:italic> deletion resulted in a hypoplastic, dysmorphic, retrognathic mandible with fully penetrant, complete cleft of the secondary palate. In contrast, most <jats:italic toggle=\"yes\">R26R</jats:italic> <jats:sup>DTA/+</jats:sup> ;mt <jats:italic toggle=\"yes\">Hand2</jats:italic> <jats:sup>Cre</jats:sup> embryos showed a reduced mandible (micrognathia) but also displayed a dramatically smaller tongue (microglossia). With a nonobstructing tongue, these mutants exhibited horizontal palatal shelf growth and formed a normal palate. These results are consistent with a mechanism in which physical obstruction by the tongue is the primary cause of downward development of the palatal shelves, far earlier than previously appreciated, and that normal elevation occurs by the release of elastic prestress. We experimentally demonstrate that in PRS, micrognathia causes cleft palate through tongue malpositioning that prevents release of this palatal shelf prestress.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"30 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149078077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How Patients Define Quality in Dental Care: A Qualitative Systematic Review","authors":"A.H.C. Tay, D.Y. Chow, E. Ng, S.M.S. Chen, Y.J. Ong, J.R.H. Tay","doi":"10.1177/00220345261474417","DOIUrl":"https://doi.org/10.1177/00220345261474417","url":null,"abstract":"Patient-centered care is central to high-quality health care, yet systems-level understanding of how patients themselves define dental care quality remains limited. This systematic review synthesized qualitative evidence using the Donabedian framework to examine patients’ perceptions of dental care and to identify core elements of quality as defined by the patients themselves. A comprehensive search of 6 databases was conducted up to July 2025. Qualitative and mixed-methods studies examining patients’ views, experiences, and values related to dental care delivery were included. Study quality was appraised using the Joanna Briggs Institute Critical Appraisal tool, and data were synthesized using thematic synthesis. Thirty-six studies spanning diverse populations and dental care contexts were included. Four analytical themes characterized how patients define quality dental care: (1) organizational and structural conditions shaping confidence in dental care; (2) feeling safe in experienced hands, reflecting perceived clinical competence; (3) interpersonal care as the foundation of perceived quality, encompassing respect, empathy, communication, and shared decision-making; and (4) relational continuity as a patient-relevant outcome over time. Patients interpret relational, emotional, and systemic features of care as symbolic indicators of health system orientation, particularly in contexts in which technical competence is difficult to assess. These indicators accumulate across encounters, shaping expectations of future care and influencing engagement, particularly among populations at greater risk of disengagement, highlighting the centrality of relational and organizational dimensions in patient-defined dental care quality.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"64 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148898902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ARID1A Mediates ROS-Induced Osteoclast Activation in TMJ Osteoarthritis","authors":"Y.L. Liu, J.Y. Xu, Y.Q. Wu, Y.L. Huang, E.H. Yao, M.L. Zhou, M.L. Mei, P. R. Cooper, J.H. Du, X.Q. Jiang","doi":"10.1177/00220345261471409","DOIUrl":"https://doi.org/10.1177/00220345261471409","url":null,"abstract":"Excessive osteoclast activation drives rapid subchondral bone destruction, serving as a critical early-stage event precipitating temporomandibular joint osteoarthritis (TMJ-OA). Although epigenetic remodeling is widely recognized as an important interface between pathological environmental signals and genomic response, the specific epigenetic mechanisms translating TMJ-OA–associated stimulation into pathological osteoclast activation remain to be elucidated. Here, using a mechanically induced TMJ-OA mouse model, we identify aberrant reactive oxygen species (ROS) accumulation as a critical upstream driver initiating excessive osteoclast activation and subsequent joint deterioration. By integrating transcriptomic and epigenomic analyses, we delineate the chromatin remodeler AT-rich interaction domain 1A (ARID1A) as an essential oxidative stress sensor within the osteoclast lineage. Mechanistically, ROS accumulation induces ARID1A upregulation and recruitment to the <jats:italic toggle=\"yes\">Src</jats:italic> enhancer, transcriptionally activating <jats:italic toggle=\"yes\">Src</jats:italic> and amplifying PI3K-AKT signaling to drive pathological osteoclastogenesis. Conditional knockout of <jats:italic toggle=\"yes\">Arid1a</jats:italic> in myeloid cells effectively abrogates subchondral bone loss and cartilage destruction in TMJ-OA. Translating these mechanistic insights, we engineered an ROS-responsive, osteoclast-targeting hydrogel for the on-demand delivery of an ARID1A-dependent canonical BRG1/BRM-associated factor complex inhibitor, which successfully alleviates TMJ-OA progression. Our findings establish the epigenetic response to ROS accumulation as a key pathogenic mechanism in TMJ-OA and highlight ARID1A as a promising therapeutic target for early disease intervention.","PeriodicalId":15596,"journal":{"name":"Journal of Dental Research","volume":"35 1","pages":""},"PeriodicalIF":7.6,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148898906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}