Journal of Drug Targeting最新文献_第2页

Engineering rifampicin-encapsulated inhalable microparticles for precision tuberculosis therapy: in vivo distribution and therapeutic evaluation. 工程利福平包封可吸入微粒用于精确治疗肺结核：体内分布和治疗评价。

IF 3.9 4区医学

Journal of Drug Targeting Pub Date : 2025-09-18 DOI: 10.1080/1061186X.2025.2559006

Ruchi Tiwari, Patibandla Jahnavi, Dhakshnamoorthy Vellingiri, Saroj Yadav, Aniruddha B Jadhav, Tatapudi Naga Aparna, V Sekar, Pankaj Sharma

{"title":"Engineering rifampicin-encapsulated inhalable microparticles for precision tuberculosis therapy: in vivo distribution and therapeutic evaluation.","authors":"Ruchi Tiwari, Patibandla Jahnavi, Dhakshnamoorthy Vellingiri, Saroj Yadav, Aniruddha B Jadhav, Tatapudi Naga Aparna, V Sekar, Pankaj Sharma","doi":"10.1080/1061186X.2025.2559006","DOIUrl":"10.1080/1061186X.2025.2559006","url":null,"abstract":"Tuberculosis (TB) continues to cause significant global mortality, highlighting the need for improved drug delivery systems. The objective of this paper focuses in describing the formulation, optimisation and in vivo assessment of rifampicin encapsulated PLGA microparticles for site-specific inhalation therapy. Microparticles for inhalation were produced by spray drying, and the DoE methodology was applied to reach the most suitable aerodynamic properties (mass median aerodynamics diameter (MMAD) 2.5 µm, fine particle fraction (FPF) 62%). Microparticles encapsulation of rifampicin led to prolongation of the pulmonary residence time both in BALB/c mice and Wistar rats and was 2.4 times higher than the concentration of the free oral rifampicin. Further, via Computational Fluid Dynamics (CFD) simulations and the use of AI-determined predictive modelling, aerosol deposition was maximised at an inhalation flow rate of 30 L/min targeting the alveolar region indicated by having 52.8% of the aerosol deposition at this region. The TB-infected mice, which showed the lung tissue bacterial load was reduced to 3.2 log colony forming unit (CFU) and the levels of TNF-α were decreased while IL-10 levels were increased. With this kind of accelerated stability testing it was ascertained that the type of formulation had a shelf-life of 24 months.","PeriodicalId":15573,"journal":{"name":"Journal of Drug Targeting","volume":" ","pages":"1-21"},"PeriodicalIF":3.9,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethosome-based delivery of betanin and curcumin for improved anti-inflammatory efficacy. 以乙醇体为基础递送甜菜素和姜黄素提高抗炎疗效。

IF 3.9 4区医学

Journal of Drug Targeting Pub Date : 2025-09-18 DOI: 10.1080/1061186X.2025.2559998

Sangameshwar B Kanthale, Vaibhav Bharad, Prakash Kendre, Ashish Tale, Sachin Borikar, Shirish Jain

{"title":"Ethosome-based delivery of betanin and curcumin for improved anti-inflammatory efficacy.","authors":"Sangameshwar B Kanthale, Vaibhav Bharad, Prakash Kendre, Ashish Tale, Sachin Borikar, Shirish Jain","doi":"10.1080/1061186X.2025.2559998","DOIUrl":"10.1080/1061186X.2025.2559998","url":null,"abstract":"Natural phytoconstituents such as betanin and curcumin have attracted interest for their significant antioxidant and anti-inflammatory properties. Their therapeutic efficacy is notably constrained by inadequate bioavailability and reduced skin permeability. The current study developed an ethosome-based gel system for the delivery of betanin and curcumin, with the objective of improving transdermal penetration and providing sustained anti-inflammatory effects. Ethosomes were formulated by cold method and optimised for physicochemical characteristics including particle size, zeta potential and entrapment efficiency. The optimised formulation exhibited a mean particle size of 202.8 nm, a zeta potential of -56.4 mV and high entrapment efficiencies of 80% for betanin and 85% for curcumin. The characterisation using SEM and FT-IR confirmed the successful encapsulation and structural integrity of the ethosomes. The ethosomal gel demonstrated an optimal pH of 6.5, pseudoplastic viscosity and superior spreadability, making it appropriate for topical use. In vitro diffusion studies demonstrated a sustained release profile lasting 8 h. Skin irritation tests confirmed its biocompatibility. The in vivo anti-inflammatory efficacy was assessed in rats by utilising carrageenan model of inflammation, results showed that ethosomal gel significantly attenuated inflammation in animals. These findings indicate that the ethosome preparation represents a promising approach for enhancing anti-inflammatory efficacy.","PeriodicalId":15573,"journal":{"name":"Journal of Drug Targeting","volume":" ","pages":"1-12"},"PeriodicalIF":3.9,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redox responsive functionalised mesoporous silica nanoparticle for targeted drug delivery of doxorubicin. 氧化还原反应功能化介孔二氧化硅纳米颗粒靶向药物递送阿霉素。

IF 3.9 4区医学

Journal of Drug Targeting Pub Date : 2025-09-17 DOI: 10.1080/1061186X.2025.2560592

Seyedeh Sahar Mojtabazadeh, Mahnoosh Samadi, Sahra Perseh, Saba Saei, Reza Bafkari, Fatemeh Atyabi, Rassoul Dinarvand

{"title":"Redox responsive functionalised mesoporous silica nanoparticle for targeted drug delivery of doxorubicin.","authors":"Seyedeh Sahar Mojtabazadeh, Mahnoosh Samadi, Sahra Perseh, Saba Saei, Reza Bafkari, Fatemeh Atyabi, Rassoul Dinarvand","doi":"10.1080/1061186X.2025.2560592","DOIUrl":"https://doi.org/10.1080/1061186X.2025.2560592","url":null,"abstract":"Mesoporous silica nanoparticles (MSNs) have been studied for the delivery of anticancer drugs because of their unique mesoporous channels. In this study, Biotin was used as a targeting moiety of MSNs for the purpose of breast cancer cell targeting, and then, Gelatine grafting onto the surface of MSNs was carried out using glutaraldehyde-mediated cross-linking as a capping layer. Dynamic light scattering (DLS), Zeta potential change, infra-red spectroscopy (FT-IR), nitrogen adsorption and desorption (BET), and transmission electron microscopy (TEM) was used for the characterisation of size, morphology and other features related to the fabricated nanoparticles (NPs). The gelatine/biotin coated MSNs (MSN@Bio-Gel) were loaded with Doxorubicin (DOX), followed by assessing its drug loading and release behaviour. In vitro experiments were carried out for exploring the antitumor effect of DOX-MSN@Bio-Gel. The size of NPs prepared in this study was in the range of 178-286 nm. The MTT assay showed suitable anticancer activity of the NPs.Confocal microscopy showed that gelatine-coated, biotin-targeted MSNs had higher cell uptake into MCF-7 cancer cells.","PeriodicalId":15573,"journal":{"name":"Journal of Drug Targeting","volume":" ","pages":"1-11"},"PeriodicalIF":3.9,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145075457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curcumin liposomal nanoparticles provide superior neuroprotection by attenuating neuropathic pain, neuroinflammation and anxiety in chronic sciatic nerve injury in rats. 姜黄素脂质体纳米颗粒通过减轻慢性坐骨神经损伤大鼠的神经性疼痛、神经炎症和焦虑提供卓越的神经保护。

IF 3.9 4区医学

Journal of Drug Targeting Pub Date : 2025-09-16 DOI: 10.1080/1061186X.2025.2559999

Mohammad Ebrahim Abbaszadeh, Gila Pirzad Jahromi, Bohlol Habibi Asl, Boshra Hatef, Gholam Hossein Meftahi

{"title":"Curcumin liposomal nanoparticles provide superior neuroprotection by attenuating neuropathic pain, neuroinflammation and anxiety in chronic sciatic nerve injury in rats.","authors":"Mohammad Ebrahim Abbaszadeh, Gila Pirzad Jahromi, Bohlol Habibi Asl, Boshra Hatef, Gholam Hossein Meftahi","doi":"10.1080/1061186X.2025.2559999","DOIUrl":"10.1080/1061186X.2025.2559999","url":null,"abstract":"Background: Chronic constriction injury (CCI) of the sciatic nerve induces neuropathic pain, inflammation, oxidative stress and neurodegenerative changes, impairing sensory and emotional function. While curcumin is well recognised for its anti-inflammatory and neuroprotective properties, its therapeutic use is limited by poor bioavailability. Curcumin liposomal nanoparticles (CLNs) offer improved delivery and stability.Methods: Male rats (n = 24) were randomly assigned to control, CCI, CCI + curcumin (60 mg/kg) and CCI + CLNs (40 mg/kg) groups. CCI was induced through sciatic nerve ligation, followed by 14 days of treatment. Behavioural assessments included thermal and mechanical pain sensitivity, anxiety-like behaviour (elevated plus maze), and sciatic nerve function (SFI). Serum inflammatory (tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6)) and oxidative stress markers (malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD) and reduced glutathione (GSH)) were measured, alongside histological analysis.Results: Compared to curcumin, CLNs showed superior efficacy in improving pain thresholds, anxiety-like behaviours and SFI scores. CLNs significantly lowered TNF-α and IL-6 levels and enhanced SOD activity, with no intergroup differences in MDA, TAC or GSH. Histology confirmed nerve regeneration and reduced neurodegeneration.Conclusions: CLNs effectively alleviated neuropathic pain, reduced neuroinflammation and improved functional recovery after sciatic nerve injury, while mitigating anxiety-like behaviours.","PeriodicalId":15573,"journal":{"name":"Journal of Drug Targeting","volume":" ","pages":"1-11"},"PeriodicalIF":3.9,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

L-Carnosine loaded chondroitin sulphate functionalized proposomes as an effective tool for transdermal rheumatoid arthritis management. l -肌肽负载硫酸软骨素功能化提案作为经皮类风湿性关节炎治疗的有效工具。

IF 3.9 4区医学

Journal of Drug Targeting Pub Date : 2025-09-15 DOI: 10.1080/1061186X.2025.2557465

Mariam Zewail, Haidy Abbas, HussamElDin Y Aboukilila, Mai F Ragab, Miranda F Kamal, Passent M E Gaafar

{"title":"L-Carnosine loaded chondroitin sulphate functionalized proposomes as an effective tool for transdermal rheumatoid arthritis management.","authors":"Mariam Zewail, Haidy Abbas, HussamElDin Y Aboukilila, Mai F Ragab, Miranda F Kamal, Passent M E Gaafar","doi":"10.1080/1061186X.2025.2557465","DOIUrl":"10.1080/1061186X.2025.2557465","url":null,"abstract":"Rheumatoid arthritis (RA) is a chronic autoimmune disease that causes joint inflammation, cartilage deterioration, and oxidative stress. The study developed transdermal RA treatment with L-carnosine (CAR)-loaded chondroitin sulphate (CHS) functionalised proposomes. CHS-functionalised proposomes measured 285 ± 0.89 nm, with PDI of 0.31 ± 0.05, zeta potential of -13.6 ± 0.67 mV, and entrapment efficiency of 73.96 ± 0.87. TEM confirmed their spherical shape and homogenous CHS coating. Biphasic drug release in vitro began with 13.2% burst release in 0.5 h and over 8 h, sustained release reached 83.79%. Ex-vivo permeation results revealed that the selected formulation increased CAR flux by 30.97 folds compared to CAR gel. In vivo testing in rats with AIA model showed that group treated with selected formulation demonstrated reduced joint inflammation and soft tissue swelling that was further confirmed by X-ray radiography. ELISA results showed significant reduction in TNF-α and IL-1β and elevation in NRF2 and SOD with levels comparable to the negative control group. Histopathological investigation showed cartilage integrity and proteoglycan content similar to the negative control. The CHS-functionalised CAR-loaded proposomes improved CAR permeation, targeted inflammatory joints, and reduced oxidative stress, making them a viable non-invasive RA treatment.","PeriodicalId":15573,"journal":{"name":"Journal of Drug Targeting","volume":" ","pages":"1-16"},"PeriodicalIF":3.9,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human plasma-derived exosomal gel: a biomimetic cargo for the transdermal delivery of methotrexate. 人血浆源性外泌体凝胶：用于甲氨蝶呤经皮递送的仿生货物。

IF 3.9 4区医学

Journal of Drug Targeting Pub Date : 2025-09-11 DOI: 10.1080/1061186X.2025.2556196

Rabia Gul, Wajeeha Khalid, Muhammad Sarfraz, Yousef A Bin Jardan, Muhammad Ikram, Hamid Bashir, Nadeem Ahmad, Laraib Khan, Bazla Siddiqui, Imran Nazir, Yueshui Jiang, Muhammad Imran Amirzada

{"title":"Human plasma-derived exosomal gel: a biomimetic cargo for the transdermal delivery of methotrexate.","authors":"Rabia Gul, Wajeeha Khalid, Muhammad Sarfraz, Yousef A Bin Jardan, Muhammad Ikram, Hamid Bashir, Nadeem Ahmad, Laraib Khan, Bazla Siddiqui, Imran Nazir, Yueshui Jiang, Muhammad Imran Amirzada","doi":"10.1080/1061186X.2025.2556196","DOIUrl":"10.1080/1061186X.2025.2556196","url":null,"abstract":"The aim of the study was to explore the potential of human plasma-derived exosomal gel as a carrier for transdermal drug delivery. Exosomes were isolated from human plasma through a combination of ultracentrifugation and dialysis techniques. Methotrexate (MTX), a weak acid drug with log P 1.53 (low permeability), was utilised as a model drug. MTX was loaded into exosomes using the freeze-thaw method. MTX-loaded exosomes were incorporated into a gel, employing carbopol 940 as a gelling agent. MTX loaded exosomes exhibited a mean size of 162.15 ± 4.21 nm, a polydispersity index (PDI) 0.372 ± 0.024, and a zeta potential of -30.6 ± 0.71 mV. Exosomal gel displayed good physicochemical properties along with desirable rheological behaviour that eased skin application. MTX-loaded exosomal gel exhibited sustained release of 59.14 ± 0.812% of the drug within 72 h at pH 7.4 as compared to nonexosomal gel p < 0.0001. MTX-loaded exosomal gel demonstrated a three-fold increase in skin permeability as compared to MTX loaded gel. Moreover, results of in-vivo studies on the carrageenan-induced inflammation model indicated exosomal gel and MTX loaded exosomal gel reduced inflammation as compared to MTX gel. These findings suggested the potential of exosomes as an emerging platform for transdermal drug delivery, offering enhanced skin penetration.","PeriodicalId":15573,"journal":{"name":"Journal of Drug Targeting","volume":" ","pages":"1-16"},"PeriodicalIF":3.9,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting lipopolysaccharides in gram-negative sepsis: therapeutic advances and challenges. 靶向脂多糖治疗革兰氏阴性脓毒症：治疗进展和挑战。

IF 3.9 4区医学

Journal of Drug Targeting Pub Date : 2025-09-10 DOI: 10.1080/1061186X.2025.2546487

Oluwakorede Akele, Freeha Rana, Sudeep Acharya, David LeDoux, Michel Chalhoub

{"title":"Targeting lipopolysaccharides in gram-negative sepsis: therapeutic advances and challenges.","authors":"Oluwakorede Akele, Freeha Rana, Sudeep Acharya, David LeDoux, Michel Chalhoub","doi":"10.1080/1061186X.2025.2546487","DOIUrl":"10.1080/1061186X.2025.2546487","url":null,"abstract":"Gram-negative bacterial sepsis remains a major global health threat, exacerbated by rising antimicrobial resistance and limited efficacy of current therapies. Central to its pathogenesis is lipopolysaccharide (LPS), a potent endotoxin that triggers overwhelming inflammation and organ dysfunction. This review critically evaluates emerging therapies targeting LPS in sepsis. Key strategies include antibiotics disrupting LPS biosynthesis and transport (e.g. zosurabalpin, darobactin), monoclonal and bispecific antibodies, extracorporeal endotoxin removal devices, and novel agents like LpxC inhibitors and nanotechnology-based platforms. Despite promising preclinical and early clinical data, translation to practice is limited by pharmacokinetic challenges, toxicity, resistance mechanisms, and inadequate patient stratification. Anti-LPS antibodies and polymyxins have shown selective benefits but face setbacks in broader trials. Nanotherapeutics and targeted filtration systems like oXiris® and Alteco® offer adjunctive potential but require validation through randomised studies. The complexity of LPS biology and sepsis heterogeneity demonstrates the need for precision medicine approaches and biomarker-guided interventions. Addressing scalability, regulatory hurdles, and cost-effectiveness will be critical to integrating LPS-targeted therapies into standard sepsis care. This review outlines a translational roadmap to harness these innovations and improve outcomes in Gram-negative sepsis.","PeriodicalId":15573,"journal":{"name":"Journal of Drug Targeting","volume":" ","pages":"1-13"},"PeriodicalIF":3.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mannosylated chitosan-decorated PLGA nanoparticles for targeted pulmonary delivery of isoniazid: a promising approach in the treatment of tuberculosis. 甘露糖基化壳聚糖修饰的PLGA纳米颗粒靶向肺递送异烟肼：治疗结核病的一种有前途的方法。

IF 3.9 4区医学

Journal of Drug Targeting Pub Date : 2025-09-10 DOI: 10.1080/1061186X.2025.2554761

Riffat Maqbool, Dur E Nayab, Muhammad Mubeen, Hussain Ali, Salman Khan

{"title":"Mannosylated chitosan-decorated PLGA nanoparticles for targeted pulmonary delivery of isoniazid: a promising approach in the treatment of tuberculosis.","authors":"Riffat Maqbool, Dur E Nayab, Muhammad Mubeen, Hussain Ali, Salman Khan","doi":"10.1080/1061186X.2025.2554761","DOIUrl":"10.1080/1061186X.2025.2554761","url":null,"abstract":"Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tb), represents a significant challenge to global health. The management of the disease requires an extended course of antibiotic therapy, spanning a duration of 6 to 9 months. The complexity and duration of these regimens frequently lead to significant adverse effects, gastrointestinal issues, and the development of drug resistance. To address these challenges, the nanoparticulate based inhalable drug delivery system was designed as such by synthesising mannosylated chitosan decorated PLGA nanoparticles loaded with isoniazid (MC-PLGA-INH-PNPs) for targeted pulmonary delivery. Hence, nanoparticle based drug delivery system offers the potential to target and deliver the loaded drug directly into the M.tb infected cells. The prepared and optimised nano-formulation had a particle size of 154.9 ± 21 nm, zeta potential -23.2 ± 0.52 mV and entrapment efficiency of 79.8% ± 0.45. Additionally, the MC-PLGA-INH-PNPs exhibited a sustained drug release profile at physiological pH 7.4 for a period of 24 hr. An in vivo study of the MC-PLGA-INH-PNPs was performed on a mouse model utilising lipopolysaccharide as an inducer. The data obtained from the in vivo studies showed substantial improvements in lung tissues architecture and reduced inflammation. The group of animals treated with the MC-PLGA-INH-PNPs showed significant improvement in restoration of the disease when compared to pure drug treated group. These findings further indicate that these inhalable MC-PLGA-INH-PNPs hold a promising strategy for the treatment of tuberculosis and considerably improves pulmonary drug delivery to the target site. However, detailed investigations and testing of this nano-formulation on other relevant animal models will be essential to successfully translate this concept from laboratory to clinical practice.","PeriodicalId":15573,"journal":{"name":"Journal of Drug Targeting","volume":" ","pages":"1-14"},"PeriodicalIF":3.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioengineered nanocomposite bacitracin zinc-loaded chitosan microspheres containing keratin/gelatine films for wound healing. 含角蛋白/明胶膜的细菌肽锌壳聚糖微球的生物工程纳米复合材料伤口愈合。

IF 3.9 4区医学

Journal of Drug Targeting Pub Date : 2025-09-05 DOI: 10.1080/1061186X.2025.2554758

Payal Shetty, Marina Koland, Suprit D Saoji, Mohammad Adnan Raza, Nilesh R Rarokar

{"title":"Bioengineered nanocomposite bacitracin zinc-loaded chitosan microspheres containing keratin/gelatine films for wound healing.","authors":"Payal Shetty, Marina Koland, Suprit D Saoji, Mohammad Adnan Raza, Nilesh R Rarokar","doi":"10.1080/1061186X.2025.2554758","DOIUrl":"10.1080/1061186X.2025.2554758","url":null,"abstract":"This study sought to create and characterize a novel antibiotic-loaded keratin-based film bandage for enhanced wound healing. Using the solvent casting method, keratin from chicken feathers was combined with gelatin (KG) in varying ratios to form films. Chitosan microspheres (Mc) were incorporated to achieve sustained release of bacitracin zinc (BZ). The microspheres were evaluated for particle size distribution, encapsulation efficiency, and in vitro drug release kinetics. The optimized film showed a controlled release profile with nearly 76% cumulative drug release over time. Embedding antibiotic-loaded microspheres within the keratin-gelatin matrix enabled prolonged delivery at the wound site, preventing infection and accelerating healing. In vivo excision wound studies demonstrated that the BZ-Mc-KG film achieved complete wound closure by day 20, significantly outperforming the disease control (p < .05). Comparative results indicated that microsphere-loaded gelatin films achieved 90% closure (p < .05), while free drug-loaded keratin-gelatin films reached 98% closure (p < .05). Slower healing was observed with drug-free keratin-gelatin films and standard mupirocin ointment (2.0% w/w). These findings highlight the synergistic potential of chicken feather keratin with BZ, supporting its application as a sustainable biomaterial for advanced wound dressings and effective therapeutic wound care strategies.","PeriodicalId":15573,"journal":{"name":"Journal of Drug Targeting","volume":" ","pages":"1-18"},"PeriodicalIF":3.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan-β-glycerophosphate thermogelling microneedles for transdermal contraceptive delivery of levonorgestrel. 壳聚糖-β-甘油磷酸酯热凝胶微针经皮给药左炔诺孕酮。

IF 3.9 4区医学

Journal of Drug Targeting Pub Date : 2025-09-05 DOI: 10.1080/1061186X.2025.2551816

Chethan Kumar K B, Sateesha S B, Ankith N A, Rajamma A J, Durgashree Diwakar, Girija E K, Likhitha C N

{"title":"Chitosan-β-glycerophosphate thermogelling microneedles for transdermal contraceptive delivery of levonorgestrel.","authors":"Chethan Kumar K B, Sateesha S B, Ankith N A, Rajamma A J, Durgashree Diwakar, Girija E K, Likhitha C N","doi":"10.1080/1061186X.2025.2551816","DOIUrl":"10.1080/1061186X.2025.2551816","url":null,"abstract":"This study presents the development of dissolving levonorgestrel-loaded microneedles (LMNs) incorporating a chitosan-β-glycerophosphate thermogelling system for sustained transdermal delivery of levonorgestrel (LNG) as a contraceptive. Polyvinylpyrrolidone K90 and Dextran 40 were included to enhance mechanical strength and controlled drug release. LMNs fabricated using poly dimethyl siloxane moulds exhibited uniform, sharp structures as confirmed by scanning electron microscopy. Fourier transform infra-red and X-ray diffraction analyses demonstrated chemical compatibility and physical stability of LNG within the matrix. The optimised LMNs showed significant mechanical strength (p < 0.05) and high insertion efficiency (F = 17.83, p = 3.03 × 10-8) across Parafilm® layers and fully dissolved within 30 min in porcine skin. Ex vivo studies revealed sustained LNG release (70.86% ± 0.42%) over 48 h, outperforming a topical gel (42.33% ± 0.91%). Drug release followed first-order kinetics (R2 = 0.996) and non-Fickian diffusion (n = 0.79), indicating a combined diffusion-erosion mechanism. In vivo evaluation in Wistar rats showed significant contraceptive efficacy, with reduced implantation sites (0.5 ± 0.55) and uterine thickness (3.66 ± 0.51 mm; p < 0.0001), comparable to oral LNG. These results highlight LMNs as a promising, minimally invasive platform for long-acting transdermal contraception, offering improved bioavailability, patient compliance and therapeutic effectiveness.","PeriodicalId":15573,"journal":{"name":"Journal of Drug Targeting","volume":" ","pages":"1-13"},"PeriodicalIF":3.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0