Journal of Comparative Pathology最新文献

{"title":"Multicentric diffuse large B-cell lymphoma with dural involvement in a nursery piglet","authors":"Fernanda F. Perosa ,&nbsp;Jean C.O. Menegatt ,&nbsp;Anderson H. Gris ,&nbsp;Manoela M. Piva ,&nbsp;Marcele B. Bandinelli ,&nbsp;Saulo P. Pavarini ,&nbsp;David Driemeier","doi":"10.1016/j.jcpa.2026.01.006","DOIUrl":"10.1016/j.jcpa.2026.01.006","url":null,"abstract":"<div><div>Lymphoma is the most frequently diagnosed neoplasm in pigs, predominantly of B-cell origin. Multicentric forms commonly affect the lymph nodes, spleen, liver and kidneys. This report describes a case of a multicentric diffuse large B-cell lymphoma with dural involvement in a 70-day-old nursery piglet presenting with hindlimb paralysis. Necropsy and histological examinations revealed nodular thickening of the meninges of the brain and sacral spinal cord, as well as adjacent paravertebral ganglia, by proliferations of large neoplastic lymphocytes. The same neoplastic cells infiltrated the mesenteric, inguinal and mediastinal lymph nodes, in addition to the spleen, liver and kidneys. Immunohistochemistry confirmed B-cell lineage, with cytoplasmic and membrane immunolabelling for CD20 and absence of labelling for CD3. To the authors’ knowledge, this is the first reported case of a multicentric lymphoma in a domestic pig with concurrent dural involvement.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"224 ","pages":"Pages 25-29"},"PeriodicalIF":0.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OBITUARY - Geoffrey Robin Smith (qualified London 1953), DVSM, DipBact, PhD, MRCVS","authors":"","doi":"10.1016/j.jcpa.2026.01.002","DOIUrl":"10.1016/j.jcpa.2026.01.002","url":null,"abstract":"","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"224 ","pages":"Pages 34-35"},"PeriodicalIF":0.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and immunohistochemical features of a feline sarcomatoid renal cell carcinoma","authors":"Thaiza Savaris,&nbsp;Daniel McGilp,&nbsp;Chiara Palmieri","doi":"10.1016/j.jcpa.2026.01.009","DOIUrl":"10.1016/j.jcpa.2026.01.009","url":null,"abstract":"<div><div>Sarcomatoid renal cell carcinoma (SRCC) is a rare and poorly characterized neoplasm in domestic animals. This report describes the clinicopathological and immunohistochemical findings of SRCC in a 7-year-old male Domestic Shorthair cat presenting with progressive weight loss, azotaemia and right-sided renomegaly. At necropsy, the right kidney was diffusely enlarged and replaced by a firm, pale-tan, poorly demarcated mass. Histologically, the renal parenchyma was effaced by a pleomorphic neoplasm composed of polygonal, spindle-shaped and multinucleated cells arranged in irregular cords and bundles. Immunohistochemically, all neoplastic cell populations were strongly positive for vimentin and labelled variably for cytokeratin AE1/AE3, while spindle and multinucleated cells also labelled for ionized calcium-binding adaptor molecule 1 (Iba-1). Anti-smooth muscle actin labelled strongly in spindle cells, whereas antibodies to CK7, high molecular weight cytokeratin, Melan A, p63 and uroplakins II and III failed to label. Based on morphological and immunophenotypic features, the tumour was diagnosed as a SRCC. The histological and immunohistochemical patterns observed closely resemble those described in human SRCC, reinforcing the comparative value of this entity. This report expands the limited knowledge of feline SRCC and highlights the importance of a focused immunohistochemical panel comprised of antibodies to vimentin, cytokeratin AE1/AE3 and Iba-1 for the accurate diagnosis of undifferentiated renal neoplasms in cats.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"224 ","pages":"Pages 16-19"},"PeriodicalIF":0.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sézary syndrome arising from cutaneous epitheliotropic T-cell lymphoma, resembling human folliculotropic mycosis fungoides, in a dog","authors":"Milena C. Paz ,&nbsp;Igor R. Santos ,&nbsp;Cassiane E. Lopes ,&nbsp;Gabriela Hartmann ,&nbsp;Rafaela B.M. Crato ,&nbsp;Aline H. Moure ,&nbsp;Marcele B. Bandinelli ,&nbsp;Gabriela Ledur ,&nbsp;Saulo P. Pavarini","doi":"10.1016/j.jcpa.2026.01.010","DOIUrl":"10.1016/j.jcpa.2026.01.010","url":null,"abstract":"<div><div>A 9-year-old neutered male Pug dog presented with a history of chronic dermatitis. Dermatological examination revealed skin thickening with keratin fronds, erythematous macules and generalized lymphadenopathy. Haematology revealed leucocytosis with lymphocytosis and medium to large neoplastic lymphocytes having cerebriform nuclei (Sézary cells). Fine-needle aspirate samples from the skin and the superficial lymph nodes contained pleomorphic medium-sized lymphocytes and a cytological diagnosis of lymphoma was made. The dog was euthanized. In addition to the cutaneous lesions, post-mortem examination revealed lymphadenomegaly, splenomegaly and hepatomegaly. Histological examination of the skin revealed a neoplastic proliferation of large lymphocytes infiltrating the deep dermis and surrounding the hair follicles. Similar neoplastic cells were present in the lymph nodes, spleen, liver and bone marrow. Neoplastic cells were immunoreactive for CD3 and not for PAX-5. The final diagnosis was Sézary syndrome arising from cutaneous epitheliotropic T-cell lymphoma resembling human folliculotropic mycosis fungoides.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"224 ","pages":"Pages 20-24"},"PeriodicalIF":0.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical demonstration of phocine herpesvirus-1 antigen in harbour seals (Phoca vitulina) of the North Sea","authors":"Bianca Kühl ,&nbsp;Monica Mirolo ,&nbsp;Valéria A. Lima ,&nbsp;Ana Rubio-Garcia ,&nbsp;Martin Ludlow ,&nbsp;Albert D.M.E. Osterhaus ,&nbsp;Wolfgang Baumgärtner ,&nbsp;Andreas Beineke ,&nbsp;Peter Wohlsein","doi":"10.1016/j.jcpa.2026.01.005","DOIUrl":"10.1016/j.jcpa.2026.01.005","url":null,"abstract":"<div><div>Phocine herpesvirus-1 (PhHV-1) can cause acute pneumonia and hepatitis with high morbidity and frequently fatal outcomes in young harbour seals (<em>Phoca vitulina</em>) and, occasionally, in grey seals (<em>Halichoerus grypus</em>). Here, we report the virological and pathomorphological findings of a PhHV-1 infection in 10 out of 90 investigated young harbour seals, collected between January 2020 and December 2021 from the Dutch North Sea coast, found either dead or suffering from severe respiratory distress resulting in euthanasia. Macroscopic findings included poor nutritional condition, poorly deflated and slightly consolidated lungs, as well as a friable and pale liver parenchyma. Moreover, six seals showed several co-morbidities such as septicaemia, pancreatitis and pneumothorax. Microscopic investigation revealed predominantly a non-suppurative peribronchitis and necrotizing bronchitis. Multifocal to coalescing necrotizing hepatitis and non-suppurative inflammation of the liver were the most striking lesions. Eosinophilic intranuclear inclusion bodies were seen in bronchial epithelial cells and hepatocytes in three and six cases, respectively. Using a monoclonal anti-PhHV-1 glycoprotein B antibody, viral antigen was detected <em>in situ</em>, specifically intra- and perilesional, in the lung and/or liver of eight seals. Electron microscopy of two selected cases demonstrated numerous intranuclear viral capsids and, within the cytoplasm, enveloped mature virions. A pan-<em>Herpesviridae</em> polymerase chain reaction using a degenerative primer set was used to confirm PhHV-1 infection. Genome fragments were detected in lung and liver samples. Collectively, these data indicate that PhHV-1 infection was the most likely cause of disease in these 10 animals, although several co-morbidities were also present. This indicates that monitoring of PhHV-1 infection status in harbour seals, especially juvenile animals, is an integral part of population health surveillance of susceptible phocid populations.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"224 ","pages":"Pages 10-15"},"PeriodicalIF":0.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serous fat atrophy in forensic veterinary pathology of domestic mammals","authors":"Amanda Ø. Birk,&nbsp;Cecilie B. Becker,&nbsp;Henrik E. Jensen","doi":"10.1016/j.jcpa.2025.12.001","DOIUrl":"10.1016/j.jcpa.2025.12.001","url":null,"abstract":"<div><div>Serous fat atrophy is the result of prolonged energy insufficiency and represents an important finding to confirm a diagnosis of emaciation in forensic veterinary pathology. The objective of this study was to investigate the presence of serous fat atrophy at multiple anatomical sites in different mammalian species in order to identify the most suitable sampling locations when emaciation is suspected. In addition, the study evaluated different fixation methods and a panel of histochemical stains to improve the histopathological diagnosis of serous fat atrophy. In all species included, bone marrow was the most reliable site for demonstrating serous fat atrophy, often followed by the adipose tissue surrounding the knee joint. In the carnivorous species, epicardial adipose tissue was limited, yielding a space or an unidentifiable amount of extracellular material. Retrobulbar adipose tissue was also suboptimal for evaluating serous fat atrophy in several species. Fixation in 10% buffered formalin produced stronger metachromatic staining with Toluidine blue compared with Carnoy's solution, indicating superior preservation of glycosaminoglycans in the tissue. While multiple histochemical stains were useful in showing the formation of glycosaminoglycans, the metachromatic stains ease the identification process, making them a preferable option. Therefore, when performing forensic necropsy of an animal suspected of emaciation, bone marrow should be considered the tissue of choice for identification of serous fat atrophy, the tissue should be fixated in 10% buffered formalin and metachromatic stains are recommended for optimal visualization.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"223 ","pages":"Pages 5-12"},"PeriodicalIF":0.9,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145917601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of tumour-infiltrating immune cells as a prognostic factor in feline oral squamous cell carcinoma","authors":"Arief P. Mihardi ,&nbsp;Alfarisa Nururrozi ,&nbsp;Masanao Ichimata ,&nbsp;Yumiko Kagawa ,&nbsp;Masaya Igase ,&nbsp;Takuya Mizuno","doi":"10.1016/j.jcpa.2026.01.004","DOIUrl":"10.1016/j.jcpa.2026.01.004","url":null,"abstract":"<div><div>This study aimed to evaluate the prognostic significance of tumour-infiltrating immune cells in feline oral squamous cell carcinoma (OSCC). Immunohistochemistry was used to quantify tumour-infiltrating immune cells in 20 feline OSCC samples. CD3⁺ T, CD8⁺ T, Foxp3⁺ regulatory T and CD20⁺ B cells and CD204⁺ macrophages were assessed in both the tumour and stroma areas. Immune cell infiltration was more prominent in the stroma area, particularly for CD20⁺ B cells. High CD3⁺ T-cell and CD20⁺ B-cell densities were negatively correlated with survival outcomes. Moreover, based on infiltration level, low densities of CD3⁺ T cell infiltration were significantly associated with longer overall survival times. Likewise, low densities of CD8⁺ T cells were linked with significantly longer progression-free intervals.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"223 ","pages":"Pages 22-29"},"PeriodicalIF":0.9,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145989712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of feline infectious peritonitis using the cell tube block technique – a pilot study","authors":"Luísa Fonte-Oliveira ,&nbsp;Célia Lopes ,&nbsp;Fernanda Malhão ,&nbsp;Ana Canadas-Sousa ,&nbsp;Hugo Gregório ,&nbsp;Ricardo Marcos ,&nbsp;Marta Santos","doi":"10.1016/j.jcpa.2025.11.003","DOIUrl":"10.1016/j.jcpa.2025.11.003","url":null,"abstract":"<div><div>Feline infectious peritonitis (FIP) is caused by a feline coronavirus (FCoV) and it is one of the most important viral diseases of cats. Recently, new drugs have been developed and used to face the inexorable fatal prognosis of FIP when untreated. More than ever, a definitive ante-mortem diagnosis is crucial to identify the cats that would benefit from antiviral treatment and to avoid euthanasia of unaffected animals. Identification of FCoV in macrophages using immunohistochemistry (IHC) is the gold standard method for diagnosing FIP. In this pilot study, the role of IHC applied to cell tube blocks (CTBs) of effusion fluids of cats clinically suspected to have FIP was assessed. A prospective cohort of seven cats was enrolled. IHC results in CTBs was positive in six out seven cases. Necropsy, histology and IHC in organs/tissues confirmed FIP infection in all the seven cases. The false negative in a CTB corresponded to a case of bacterial peritonitis secondary to FIP. As proof-of-concept, CTBs are valuable as a non-invasive technique for a definitive ante-mortem/clinical diagnosis of FIP and it is recommended to obtain CTBs from effusion fluids of all suspected FIP cases.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"223 ","pages":"Pages 17-21"},"PeriodicalIF":0.9,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145819403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyserositis, meningoencephalitis, arthritis and omphalitis due to Salmonella Agona infection in a pig","authors":"Jean C.O. Menegatt ,&nbsp;Claiton I. Schwertz ,&nbsp;Vânia S. Bourckhardt ,&nbsp;Roberta T.B. França ,&nbsp;Fernanda F. Perosa ,&nbsp;Manoela M. Piva ,&nbsp;Brenda O. Silveira ,&nbsp;David Driemeier","doi":"10.1016/j.jcpa.2025.12.002","DOIUrl":"10.1016/j.jcpa.2025.12.002","url":null,"abstract":"<div><div>Bacterial polyserositis, arthritis and meningitis are well-known conditions in pigs, commonly linked to <em>Glaesserella parasuis</em>, <em>Streptococcus suis</em> and <em>Mycoplasma hyorhinis</em>. <em>Salmonella enterica</em> infections, particularly <em>S.</em> Typhimurium and <em>S.</em> Choleraesuis, typically present as enteric or septicaemic diseases. This study reports a rare case of <em>Salmonella enterica</em> subsp. <em>enterica</em> serovar Agona (<em>S.</em> Agona) infection in an 80-day-old pig presenting with polyserositis, meningoencephalitis, arthritis and omphalitis. In the bacteriological examination, a pure culture of <em>S</em><em>.</em> Agona was obtained from the fibrinous lesions, which enabled diagnosis. Additionally, immunohistochemical examination allowed confirmation of the septicaemic distribution of the bacteria. The findings suggest umbilical contamination as the probable infection route, differing from the usual fecal-oral transmission of <em>Salmonella</em>. Although non-enteric <em>Salmonella</em> infections have been documented in various species, this is the first confirmed case of <em>S.</em> Agona causing such lesions in pigs. This case highlights the importance of specific diagnostic approaches to accurately identify the aetiological agent, as clinical and pathological presentations may resemble other common bacterial infections in pigs.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"223 ","pages":"Pages 1-4"},"PeriodicalIF":0.9,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145847625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of malignant adenomyoepithelioma in a guinea pig (Cavia porcellus)","authors":"Luise K. Jubrael ,&nbsp;Angélica Consalter ,&nbsp;Tábata Maués ,&nbsp;Cátia D. de Paula ,&nbsp;Geovanni D. Cassali ,&nbsp;Maria de Lourdes G. Ferreira","doi":"10.1016/j.jcpa.2025.11.004","DOIUrl":"10.1016/j.jcpa.2025.11.004","url":null,"abstract":"<div><div>Mammary cancer is one of the most common neoplasms in women and female dogs, and it has also been found to occur with significant frequency in guinea pigs (<em>Cavia porcellus</em>). A 4-year-old female guinea pig presented with a firm, irregular shaped mass, measuring 6.6 cm in the largest axis, in the right mammary gland. Cytological examination suggested mammary carcinoma. Based on these results, a right inguinal mammectomy was performed. Histological examination revealed a biphasic neoplastic proliferation in the hypodermis consisting of epithelial cells in cribriform, papillary, tubule and nest arrangements, and malignant myoepithelial cells arranged in multidirectional bundles and nests, with marked anisocytosis and anisokaryosis. Immunolabelling was positive for GATA3 and Ckpan in the epithelial component and positive for p63 in the myoepithelial component. After 71 days, the patient developed severe dyspnoea and agitation, and chest radiography showed several rounded structures distributed multifocally throughout the lung parenchyma, the largest of them measuring 0.9 × 0.6 cm. Macroscopic and histological examinations revealed multifocal metastases in the lung parenchyma, multifocal suppurative pneumonia and severe diffuse pulmonary oedema, confirming the cause of death as respiratory failure due to lung metastases. A metastatic malignant adenomyoepithelioma was diagnosed on the basis of the macroscopic, histological and immunohistochemical findings.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"223 ","pages":"Pages 13-16"},"PeriodicalIF":0.9,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}