Journal of Comparative Pathology最新文献

{"title":"Primary intracranial squamous cell carcinoma in a mule","authors":"João R.C. Brito Junior ,&nbsp;Yanca G.S. Soares ,&nbsp;Laynaslan A. Soares ,&nbsp;Ismael L. Borges ,&nbsp;Rodrigo C. Alves ,&nbsp;Daniel M. Assis ,&nbsp;Glaucia D. Kommers ,&nbsp;Antonio F.M. Dantas ,&nbsp;Glauco J.N. Galiza","doi":"10.1016/j.jcpa.2025.03.001","DOIUrl":"10.1016/j.jcpa.2025.03.001","url":null,"abstract":"<div><div>Primary intracranial squamous cell carcinoma is a malignant neoplasm reported only in humans and usually originates from epidermoid or dermoid cysts. We describe the first case of primary intracranial squamous cell carcinoma in an animal, emphasizing its clinical, pathological and immunohistochemical findings. A 15-year-old male mule was euthanized after bilateral blindness. At necropsy, the optic nerve, optic chiasm, pituitary gland and trigeminal nerve were surrounded by a multilobulated, firm, whitish mass interspersed by yellowish, irregular, friable multifocal areas of cavitation. Histologically, the mass was formed of neoplastic pleomorphic squamous epithelial cells that formed nests and sometimes contained areas with keratin pearls. Neoplastic cells were immunopositive for cytokeratin and immunonegative for vimentin and alpha fetoprotein. We conclude that primary intracranial squamous cell carcinoma can occur in animals and result in neurological signs, and should be considered as a differential diagnosis for diseases of the central nervous system.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"218 ","pages":"Pages 37-40"},"PeriodicalIF":0.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic amyloid A amyloidosis secondary to chronic enteritis in a captive little owl (Athene noctua)","authors":"Mitsuhiro Ikeda ,&nbsp;Hirotaka Kondo ,&nbsp;Tomoaki Murakami ,&nbsp;Susumu Iwaide ,&nbsp;Yoshiyuki Itoh ,&nbsp;Aoi Onoue ,&nbsp;Rino Nishimura ,&nbsp;Aoi Kamohara ,&nbsp;Kei Imura ,&nbsp;Hisashi Shibuya","doi":"10.1016/j.jcpa.2025.02.005","DOIUrl":"10.1016/j.jcpa.2025.02.005","url":null,"abstract":"<div><div>A male little owl (<em>Athene noctua</em>), approximately 2 years old, collapsed and subsequently died. Grossly, there was hepatomegaly with minimal chalky white lesions on the serosal surface of the liver. The serosal surface of the intestines was also tan to chalky white. Histologically, amorphous eosinophilic material was deposited in the vascular walls and interstitium of many organs, including endoneurial microvessels and perivascular interstitium. These deposits were consistent with amyloid and positive for anti-serum amyloid A (SAA) antibody on immunohistochemical labelling. On mass spectrometry-based proteomic analysis, high levels of SAA were detected. This report provides descriptive details of the histopathological and immunohistochemical findings and mass spectrometry-based proteomic analysis and genetic analysis of systemic amyloidosis with deposition in the peripheral nervous system in a little owl.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"218 ","pages":"Pages 26-30"},"PeriodicalIF":0.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disseminated T-cell lymphoma in a free-living white-winged duck (Asarcornis scutulata) under human care","authors":"Michelle F. O'Brien ,&nbsp;Sara Shopland ,&nbsp;Rosa Lopez Colom ,&nbsp;Andrew F. Rich","doi":"10.1016/j.jcpa.2025.02.002","DOIUrl":"10.1016/j.jcpa.2025.02.002","url":null,"abstract":"<div><div>The white-winged duck (<em>Asarcornis scutulata</em>) is an endangered large, stocky anatid species that originates from South-East Asia. We report an adult, 9-year-old male specimen that presented with a 7-month history of intermittent lethargy and pale mucous membranes. Haematology and plasma biochemistry analyses, performed twice at a 6-week interval, revealed anaemia, lymphocytosis and raised renal and hepatic enzyme activities. These findings initially raised clinical suspicion of either avian tuberculosis (ATB), amyloidosis or neoplasia based on species predilection. Initial blood smear evaluation revealed occasional atypical lymphocytes. The duck slowly lost weight over 8 weeks, despite appearing behaviourally normal, prior to rapid deterioration (ie, lethargy and incoordination) resulting in death before euthanasia could be performed. On post-mortem examination, the bird was in poor body condition, with visceral lesions including hydropericardium, an opaquely thickened pericardium, hepatomegaly with dark-to-pale tan capsular and parenchymal mottling, and splenomegaly. On histology and immunohistochemistry, extensive T-cell lymphocytic infiltration was found throughout the pericardium, liver and spleen, with segmental infiltrates within the kidneys and small intestine. Intravascular neoplastic T cells were also seen in large numbers throughout the vascular lumina of the affected organs (indicative of metastatic dissemination). A disseminated lymphoma was favoured over a primary lymphocytic leukaemia due to the lack of lymphocytosis on haematology. This is the first report of disseminated T-cell lymphoma in this endangered duck species.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"218 ","pages":"Pages 31-36"},"PeriodicalIF":0.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathology of an unusual outbreak of fatal disease associated with mycobacterial and Pseudomonas aeruginosa infections and intracytoplasmic inclusion bodies in juvenile spectacled caimans (Caiman crocodilus)","authors":"Sufiah U.N.K. Azlin ,&nbsp;Nurul F. Muhammad Husairy ,&nbsp;Charisha Fraser ,&nbsp;Nurul I.U. Zahli ,&nbsp;Mohd N. Akmal ,&nbsp;Annas Salleh","doi":"10.1016/j.jcpa.2025.02.003","DOIUrl":"10.1016/j.jcpa.2025.02.003","url":null,"abstract":"<div><div>The spectacled caiman (<em>Caiman crocodilus</em>) is a common crocodilian species used for exhibition in zoos and for luxury leather and meat production. We investigated unusual mortality among juvenile spectacled caimans in a zoo in Malaysia over a 2-year period. Clinical signs included inappetence, poor body condition, dehydration and ocular lesions. Necropsy findings included integumentary, respiratory and renal abnormalities. Histopathological analyses revealed necrotic plugs and intracytoplasmic eosinophilic inclusion bodies in epidermal keratinocytes, granulomatous lesions in the lungs and liver and gout crystals in the kidneys. Acid-fast, beaded, rod-shaped bacteria were identified in the granulomatous lesions using Ziehl–Neelsen staining. Microbiological investigations identified <em>Pseudomonas aeruginosa</em> as the predominant pathogen, while mycobacteriosis was also detected and poxvirus infection was suspected. However, due to the lack of suitable samples, molecular diagnosis was challenging. These findings emphasize the multifactorial nature of disease in captive spectacled caimans and the need for enhanced management strategies to mitigate health risks and preserve population viability.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"218 ","pages":"Pages 19-25"},"PeriodicalIF":0.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143611583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the relationship between histological grading, fibrillar collagen alterations and nuclear phenotypes in canine mammary carcinomas","authors":"Danielle D.P. Cerce,&nbsp;Gabriela C. Cândido,&nbsp;Maysa B. de Almeida,&nbsp;Jhuan L. Silva,&nbsp;Fernanda G.G. Dias,&nbsp;Marcela A. Rodrigues","doi":"10.1016/j.jcpa.2025.02.001","DOIUrl":"10.1016/j.jcpa.2025.02.001","url":null,"abstract":"<div><div>We evaluated collagen deposition and nuclear phenotypes in non-inflammatory, metastasis-free canine mammary carcinomas at the time of tumour resection. A retrospective cohort analysis was conducted on 68 female dogs diagnosed with mammary carcinomas between January 2013 and December 2021, excluding cases of mammary sarcoma, carcinosarcoma, inflammatory mammary cancer and metastases. Tumours were classified into histological subtypes using the Peña grading system and assigned grades accordingly. Software-assisted video image analysis was utilized to quantitatively assess collagen deposition, organization and nuclear phenotypes. Histological grading was performed by three independent examiners to ensure reproducibility and minimize observer bias. Significant differences in collagen deposition and nuclear phenotypes were observed across histological grades. Grade III carcinomas had significantly greater collagen deposition, both within the tumour core and at the tumour periphery, compared with grades I and II. Collagen organization was markedly increased in grade III carcinomas. Nuclear phenotype analysis revealed distinct features that allowed clear differentiation between grade II and grade III tumours. Software-assisted image analysis effectively identified distinct patterns of collagen deposition, organization and nuclear phenotypes associated with canine mammary carcinomas of various grades, providing important information about tumour biology.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"218 ","pages":"Pages 1-11"},"PeriodicalIF":0.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of osteosarcoma and investigation of risk factors in military working dogs: a retrospective study using data from the Deceased Military Working Dog Database","authors":"Alicia Moreau ,&nbsp;Justin G. Bergeron ,&nbsp;Kelsey Fiddes ,&nbsp;Anna-Maria Travis ,&nbsp;Sharon Daye ,&nbsp;Amy K. LeBlanc ,&nbsp;Jessica A. Beck ,&nbsp;Terra D. Vincent-Hall ,&nbsp;Elise LaDouceur","doi":"10.1016/j.jcpa.2025.01.007","DOIUrl":"10.1016/j.jcpa.2025.01.007","url":null,"abstract":"<div><div>A retrospective study of military working dogs (MWDs) that were on active duty within the Department of Defense from 2000 to 2023 was conducted for the prevalence of osteosarcoma (OSA), histological subtypes and metastatic potential, and to examine potential risk factors. The prevalence of OSA in privately owned dogs (ie, pets of Department of Defense beneficiaries) that were patients of veterinary treatment facilities from 2000 to 2023 was used as a control. Between 2000 and 2023, 2,220 deceased MWD records had pathology data available for review, 27 of which had OSA (1.2% prevalence). The age at death was recorded in 22 of the 27 cases. During the same period, tissue specimens submitted to the Joint Pathology Center from privately owned dogs had pathology data available for review, and 83 were diagnosed with OSA. Breed, sex, neuter status, age at death and procurement location did not significantly affect the odds of an OSA diagnosis. MWDs that had patrol certification had decreased odds of OSA (odds ratio 0.37 with a 95% confidence interval [CI] of 0.17–0.78). When privately owned dogs were diagnosed with OSA they were unlikely to have axial OSA (22%). The corresponding figure in MWDs was much higher (45%). The occurrence of appendicular OSA was similar between MWDs (48%) and privately owned dogs (54%).</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"218 ","pages":"Pages 12-18"},"PeriodicalIF":0.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor.","authors":"","doi":"10.1016/j.jcpa.2025.01.008","DOIUrl":"https://doi.org/10.1016/j.jcpa.2025.01.008","url":null,"abstract":"","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal histoplasmosis with granulomatous colonic and mesenteric lymphadenitis due to Histoplasma mississippiense infection in an American Mammoth Jackstock donkey (Equus asinus)","authors":"Justin M. Stilwell,&nbsp;Julianne M. White,&nbsp;Clare C. Brown ,&nbsp;Amelia I. Andersson,&nbsp;Bryan Waldridge,&nbsp;Natalie K. Stilwell","doi":"10.1016/j.jcpa.2024.12.004","DOIUrl":"10.1016/j.jcpa.2024.12.004","url":null,"abstract":"<div><div>Histoplasmosis is a rarely reported clinical disease of equids in North America and is historically attributed to <em>Histoplasma capsulatum</em> var. <em>capsulatum</em>. This report details a case of intestinal histoplasmosis with lymphadenitis in an American Mammoth Jackstock donkey from Mississippi. Clinically, the donkey had chronic wasting, poor appetite, diarrhoea and an intra-abdominal mass detected by ultrasound. Grossly, the distal small and proximal large intestines contained multifocal, raised, tan, nodular, button ulcers along the mucosal surface. The associated colonic and mesenteric lymph nodes were severely enlarged, mottled tan to red and firm, and formed a large multinodular mass within the mesentery. Microscopically, lesions consisted of nodular infiltrates of epithelioid macrophages and multinucleated giant cells laden with numerous yeasts, along with mucosal ulceration and necrosis. Fungal polymerase chain reaction from lesioned intestines and lymph nodes targeting the internal transcribed spacer region yielded a 585 bp sequence that matched identically to two human isolates of <em>Histoplasma mississippiense</em>, one of several cryptic species previously classified together as <em>H. capsulatum</em> var. <em>capsulatum</em> endemic to North America. This case represents the first molecular identification of a <em>Histoplasma</em> species infecting a North American equid and the second report of histoplasmosis in a donkey from North America.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"217 ","pages":"Pages 11-15"},"PeriodicalIF":0.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nocardiosis in domestic ferrets (Mustela putorius furo)","authors":"Carles Juan-Sallés ,&nbsp;Luary C. Martínez-Chavarría ,&nbsp;Andrés Montesinos ,&nbsp;Jacobo Giner ,&nbsp;Xavier Valls ,&nbsp;Javier Bermúdez ,&nbsp;Rigoberto Hernández-Castro ,&nbsp;María Ardiaca ,&nbsp;Vicente González ,&nbsp;José Villora ,&nbsp;Alberto Marco","doi":"10.1016/j.jcpa.2024.12.001","DOIUrl":"10.1016/j.jcpa.2024.12.001","url":null,"abstract":"<div><div><em>Nocardia</em> spp are ubiquitous, gram-positive, variably acid-fast, branching and beaded filamentous, facultative intracellular bacteria that are resistant to phagocytosis and can cause localized or systemic disease in a variety of mammals, including humans, as well as in birds, fish and reptiles. Seventeen pet domestic ferrets (<em>Mustela putorius furo</em>) were diagnosed with nocardiosis by several methods including cytological evaluation, histopathology, Ziehl–Neelsen staining and polymerase chain reaction (PCR). All except two ferrets were 2 years old or older at the time of clinical presentation. Clinical findings included anorexia, weight loss, lymphadenomegaly, splenomegaly, masses/nodules in internal organs, hindlimb weakness or paresis, vomiting, dyspnoea, central nervous system signs, diarrhoea, abdominal pain and coughing. Anaemia, leucocytosis, neutrophilia, hypoalbuminaemia and hyperglobulinaemia were frequent. All ferrets had granulomatous or pyogranulomatous inflammation involving most commonly the lymph nodes, spleen, lungs, liver and adipose tissue. Intralesional acid-fast, branching filamentous bacilli were detected in 15 of 17 ferrets. PCR for the <em>16S</em> rRNA gene of <em>Nocardia</em> spp was positive in 13 of 15 ferrets, including the two that were negative by acid-fast staining; of these two ferrets, one had intralesional coronavirus antigen. BLASTn analysis of eight sequences revealed six different <em>Nocardia</em> spp, including <em>N. globerula</em>, <em>N. seriolae</em> and <em>N. donostiensis</em>, that have rarely been reported to cause disease in humans and terrestrial animals. Antibiotic treatment (most commonly trimethoprim-sulfamethoxazole alone or in combination with clarithromycin and marbofloxacin) was followed by a marked improvement in several patients, although relapses were frequent. Six ferrets had concurrent neoplasia as a potential predisposing factor. Nocardiosis should be included in the differential diagnosis of granulomatous/pyogranulomatous inflammation in ferrets, particularly in those over 2 years of age.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"217 ","pages":"Pages 1-10"},"PeriodicalIF":0.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathology of peritonitis in cattle","authors":"Eryca C. Lamego,&nbsp;Igor R. Santos,&nbsp;Milena C. Paz,&nbsp;Francisca M.S. Barbosa,&nbsp;Luciana Sonne,&nbsp;Welden Panziera,&nbsp;David Driemeier,&nbsp;Saulo P. Pavarini","doi":"10.1016/j.jcpa.2024.12.003","DOIUrl":"10.1016/j.jcpa.2024.12.003","url":null,"abstract":"<div><div>Although peritonitis is highly prevalent in cattle, there have been only limited studies on the pathology of this condition. We describe the gross and histological aspects of primary and secondary peritonitis in cattle based on necropsy reports of 46 cases. Twenty-six were female (26/46; 56.5%) and 24 male (24/46; 43.5%), 31 (31/46; 67.4%) beef breed and 15 (15/46; 32.6%) dairy breed. Twenty were 0–12 months old (20/46; 43.5%), nine were 13–36 months old (9/46; 19.6%) and 15 were &gt;36 months old (15/46; 32.6%). Two were of unknown age. Primary peritoneal tuberculosis (PT) was present in 7/46 cases (15.2%) and macroscopic lesions were mainly multifocal (6/7; 85.7%) and characterized by white to yellow, firm nodules of variable size. Histologically, they were characterized by granulomatous inflammation with intralesional acid–alcohol-resistant bacilli. All cases were positive for <em>Mycobacterium tuberculosis</em> complex and negative for <em>Mycobacterium avium</em> on immunohistochemistry. Secondary peritonitis was diagnosed in 39/46 cases (84.8%), caused by organ rupture or perforated ulcer (21/39; 53.9%), traumatic reticuloperitonitis (9/39; 23.1%), post-castration inflammation (7/39; 17.9%) or omphalophlebitis (2/39; 5.1%). Macroscopic lesions in secondary peritonitis were mainly diffuse (31/39; 79.5%) and comprised roughening of the peritoneal surface, which was covered by free white to yellow–orange fibrin strands with a liquid to pasty, opaque, white–grey effusion (30/39; 76.9%). Bacteriological culture of the exudate from 15 secondary peritonitis cases identified mainly <em>Truperella pyogenes</em> (8/15; 53.3%) and <em>Escherichia coli</em> (5/15; 33.3%). This study enhances our understanding of the pathological manifestations of peritonitis in cattle.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"217 ","pages":"Pages 16-24"},"PeriodicalIF":0.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}