Journal of Comparative Pathology最新文献

{"title":"Annual congress of the European Society of Veterinary Pathology and the European College of Veterinary Pathologists.","authors":"","doi":"10.1016/j.jcpa.2025.04.002","DOIUrl":"https://doi.org/10.1016/j.jcpa.2025.04.002","url":null,"abstract":"","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of brain lesions in Danish farm mink (Neogale vison) with acute, fatal SARS-CoV-2 infection","authors":"Michelle L. Quaade ,&nbsp;Karen M. Wegener ,&nbsp;Louise W.D. Otzen ,&nbsp;Thomas B. Rasmussen ,&nbsp;Anne S.V. Hammer","doi":"10.1016/j.jcpa.2025.04.003","DOIUrl":"10.1016/j.jcpa.2025.04.003","url":null,"abstract":"<div><div>The pathology of brain tissue from 13 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infected mink (<em>Neogale vis</em><em>on</em>), 20 mink in recovery, as well as 16 uninfected mink were investigated by histology and immunohistochemistry (IHC), contributing to the limited knowledge on brain pathology in mink. IHC and histology did not reveal direct viral infection in the brain tissue, but evidence of fibrin microthrombi were frequently found in the acute fatally infected mink, without associated ischaemic injury or inflammation. This finding does not align with observations in brains of human COVID-19 patients, as the mink lacked the ischaemic and inflammatory lesions commonly associated with the cerebral microthrombi observed in human studies. Neither fibrin microthrombi nor ischaemic or inflammatory lesions were observed in the brain of recovering mink.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"219 ","pages":"Pages 36-40"},"PeriodicalIF":0.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological findings in raccoon dogs (Nyctereutes procyonoides) in Schleswig-Holstein, Germany","authors":"Jana C. Klink ,&nbsp;Alexandra Rieger ,&nbsp;Peter Wohlsein ,&nbsp;Sophie Aurich ,&nbsp;Christiane Hoffmann ,&nbsp;Christa Ewers ,&nbsp;Marie-Kristin Raulf ,&nbsp;Christina Strube ,&nbsp;Annette Johne ,&nbsp;Pavlo Maksimov ,&nbsp;Timm Harder ,&nbsp;Dennis Rubbenstroth ,&nbsp;Laura Rehburg ,&nbsp;Gisa Gerold ,&nbsp;Robert Fux ,&nbsp;Volker Rickerts ,&nbsp;Hermann Ansorge ,&nbsp;Ursula Siebert","doi":"10.1016/j.jcpa.2025.04.004","DOIUrl":"10.1016/j.jcpa.2025.04.004","url":null,"abstract":"<div><div>Raccoon dogs (<em>Nyctereutes procyonoides</em>) originated from East Asia and are listed as invasive alien species of Union concern in the European Union. Apart from their potential negative impact on native fauna as predators and competitors, they are discussed as reservoirs and vectors for various infectious diseases. Between 2021 and 2022, comprehensive post-mortem examinations of 110 raccoon dogs were performed as part of a health and risk assessment study of selected invasive alien species in Schleswig-Holstein, Germany. Parasitic infections, often accompanied by eosinophilic and/or granulomatous inflammation, were one of the most common and significant findings. In total, 64% animals had an ectoparasitosis and up to 76% an endoparasitosis. <em>Alaria alata</em> and <em>Trichinella</em> spp were the most relevant endoparasite species found. Eggs of <em>A. alata</em> were detected in 30% of faecal samples, while the prevalence of <em>Trichinella</em> spp in muscle samples was 0.9%. Influenza A virus, canine distemper virus, rustrela virus, Borna disease virus 1 and severe acute respiratory syndrome corona virus 2 were not detected. Carnivore protoparvovirus 1 was detected with a prevalence of 3%, but was not associated with pathognomonic lesions. In general, most of the pathological findings were of minor significance regardless of whether pathogens were detected or not, and therefore did not appear to affect the overall health status of the examined animals. Our study shows that raccoon dogs may contribute to the spread and persistence of certain pathogens by acting as carriers of infectious diseases. This underpins the need to further investigate the risk they pose to endemic European wildlife, livestock and human health.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"219 ","pages":"Pages 59-77"},"PeriodicalIF":0.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forensic evaluation of tail-bite wounds in pigs in Denmark","authors":"Christoffer K. Flyger,&nbsp;Cecilie B. Becker,&nbsp;Benjamin M. Jørgensen,&nbsp;Henrik E. Jensen","doi":"10.1016/j.jcpa.2025.05.002","DOIUrl":"10.1016/j.jcpa.2025.05.002","url":null,"abstract":"<div><div>In Denmark, 32% of forensic porcine cases are due to skin lesions of which 8.1% are located at the tail. We investigated 67 pigs that had been delivered to an abattoir with tail-bite wounds considered to be a violation of the law and consequently submitted for forensic examination. The objective was to scrutinize if gross morphological characteristics could be used to allocate tails into four severity groups reflecting the degree of underlying pathology. The group allocation was based on the length and thickness of the tails: (A) almost total lack of tail; (B) short (&lt;6 cm) and thickened tail; (C) long (&gt;6 cm) and thickened tail; and (D) tail with no or minimal thickening. Selected tails were also examined histopathologically and radiologically in order to evaluate the impact of the three methods on the evaluation. Underlying pathological manifestations were similar in almost all tails, although there was a substantial difference in the extent of proliferative changes. In all tails, chronic changes including formation of granulation tissue were seen. Lesions that were not identified grossly (eg, osteomyelitis) were more often diagnosed radiologically, while soft tissue proliferation was more often disclosed histologically. Therefore, all three methods should be applied when performing a forensic evaluation of porcine tail-bite wounds.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"219 ","pages":"Pages 102-107"},"PeriodicalIF":0.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canine prostate cancer cell transcriptome reveals important dysregulation in PI3K/AKT/mTOR pathway","authors":"Priscila E. Kobayashi ,&nbsp;Patrícia F. Lainetti ,&nbsp;Antonio F. Leis-Filho ,&nbsp;Flávia K. Delella ,&nbsp;Igor S.T. Vicente ,&nbsp;Carlos E. Fonseca-Alves ,&nbsp;Renée Laufer-Amorim","doi":"10.1016/j.jcpa.2025.03.190","DOIUrl":"10.1016/j.jcpa.2025.03.190","url":null,"abstract":"<div><div>Dogs are the only large mammals, besides humans, that develop spontaneous prostate cancer, which has a poor prognosis and limited treatment efficacy. Considering the central role of mammalian target of rapamycin (mTOR) in carcinogenesis, the use of rapamycin, an mTOR inhibitor, has attracted considerable attention. In this study, we performed gene expression microarray analyses of normal canine prostate and prostate carcinoma cells. Among the 6,270 differentially expressed genes revealed in the transcriptome, 3,242 were upregulated and 3,028 were downregulated, and were related to phosphatidylinositol 3-kinase (PI3K)/AKT/mTOR pathway activation, as confirmed by enrichment analysis. Among the genes involved in this pathway, we found increased expression levels of <em>FKBP1A</em>, <em>FKBP1B</em>, <em>AKT1S1</em>, <em>PDK2</em>, <em>PIP5K1</em> and <em>PIP5KL1</em> in canine prostate cancer cells compared with normal prostate cells. We also treated two canine prostate cancer cell lines (PC1 and PC2) with rapamycin <em>in vitro</em> (6, 10 and 12 μM) for 24 h and observed a dose-dependent decrease in cell viability. Our results indicate that rapamycin significantly increased AKT transcript levels in both cell lines, indicating resistance to treatment. However, mTOR and 4E-BP1 expression were downregulated after rapamycin treatment. We suggest that mTOR inhibition is a potential treatment of choice for canine prostate cancer, which may guide and contribute to future prostate carcinoma clinical trials. However, the acquisition of resistance to treatment remains a challenge, and precision medicine may help overcome this problem.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"219 ","pages":"Pages 52-58"},"PeriodicalIF":0.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrent outbreak of Streptococcus bovis/Streptococcus equinus complex-associated meningoencephalitis in farmed mink","authors":"Megan J. Crawford ,&nbsp;Kimberley N. Sebastian ,&nbsp;Elizabeth Elsmo ,&nbsp;Lorelei L. Clarke ,&nbsp;Mayra F. Tsoi","doi":"10.1016/j.jcpa.2025.04.010","DOIUrl":"10.1016/j.jcpa.2025.04.010","url":null,"abstract":"<div><div>The <em>Streptococcus bovis</em>/<em>Streptococcus equinus</em> complex (SBSEC) is part of the normal gastrointestinal microflora in animals and humans but is an opportunistic pathogen that has been implicated in meningitis, endocarditis and septicaemia. There is only one report of SBSEC causing disease (endocarditis) in mink. This case report describes two outbreaks of SBSEC infection in farmed mink occurring 1 year apart at the same facility. In both cases, affected mink had neurological signs prior to death and the mortality level was 5,000 (2021) and 500 (2022) out of 100,000 mink. Necropsy findings included hepatomegaly, splenomegaly and congested lungs. Histopathology revealed suppurative meningoencephalitis and neutrophilic inflammation in the lungs, liver and kidneys. Aerobic bacterial culture identified SBSEC isolates in the brain and liver. This is the first report to implicate SBSEC in meningoencephalitis and septicaemia in farmed mink.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"219 ","pages":"Pages 78-81"},"PeriodicalIF":0.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143932141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multicentric B-cell lymphoma, phaeochromocytoma and amyloid-rich thyroid carcinoma in a common warthog (Phacochoerus africanus): a case of multiple endocrine neoplasia type 2?","authors":"Jemima Strutt ,&nbsp;Ian Ashpole ,&nbsp;Anna Orlowska ,&nbsp;Valerie Tilston ,&nbsp;Alberto R. Barbon ,&nbsp;Guido Rocchigiani ,&nbsp;Julian Chantrey ,&nbsp;Yannick Van de Weyer","doi":"10.1016/j.jcpa.2025.04.008","DOIUrl":"10.1016/j.jcpa.2025.04.008","url":null,"abstract":"<div><div>Neoplastic diseases are poorly documented in Suidae species. This report describes a 15-year-old female common warthog (<em>Phacochoerus africanus</em>) with multicentric lymphoma, phaeochromocytoma and C-cell carcinoma. The animal had multiple episodes of weakness and epistaxis in the months before death, until it was euthanized for acute lethargy and recumbency. Post-mortem examination revealed haemoabdomen originating from enlarged lymph nodes at the mesenteric root. The warthog had generalized lymphadenopathy, consistent with B-cell lymphoma based on CD20 immunohistochemistry. The adrenal glands were bilaterally enlarged, one by an ovoid mass arising from the medulla that effaced the cortex, consistent with a well-differentiated phaeochromocytoma, based on histomorphology and chromogranin A immunopositivity. This animal also had a unilateral, encapsulated thyroid nodule characterized by abundant interstitial calcitonin-positive amyloid and irregular tubular structures, similar to human medullary thyroid carcinoma. There was moderate metastatic calcification of the lungs and upper respiratory mucosa, presumably part of a paraneoplastic syndrome. The combination of phaeochromocytoma and an amyloid-rich thyroid carcinoma in this animal is suggestive of multiple endocrine neoplasia type 2 syndrome which, although well described in humans, is rarely reported in animals. Further investigations of genetic aspects are recommended.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"219 ","pages":"Pages 82-87"},"PeriodicalIF":0.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sinonasal angiofibroma in a horse","authors":"Magdalena Schollmeyer ,&nbsp;Elisabeth M. Liebler-Tenorio ,&nbsp;Christina Felton ,&nbsp;Stefan Kohl ,&nbsp;Reiner Ulrich","doi":"10.1016/j.jcpa.2025.05.003","DOIUrl":"10.1016/j.jcpa.2025.05.003","url":null,"abstract":"<div><div>A 34-year-old Haflinger gelding presented with a unilateral, expansile, intranasal mass that regrew after partial excision. After euthanasia, a large pedunculated mass that originated from the left caudal maxillary sinus and obliterated the left nasal cavity was seen by radiological and macroscopic examination. Histopathology revealed a poorly cellular, expansile, well-vascularized neoplasm composed of a loosely arranged meshwork of spindle cells and collagen fibres. Spindle cells were immunopositive for alpha-smooth muscle actin and occasionally for vimentin, whereas endothelial cells immunolabelled for factor VIII-related antigen. Based on clinical, radiological, macroscopic and microscopic similarities to canine and human cases, the mass was diagnosed as an angiofibroma.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"219 ","pages":"Pages 88-90"},"PeriodicalIF":0.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cutaneous melanoma with heterologous rhabdomyosarcomatous component in a dog","authors":"Rafael Ramírez-Romero","doi":"10.1016/j.jcpa.2025.04.009","DOIUrl":"10.1016/j.jcpa.2025.04.009","url":null,"abstract":"<div><div>Melanocyte-derived tumours are frequent in humans and animals and the clinicopathological progression of malignant presentations in dogs is comparable with humans. However, melanocytic tumours are a heterogeneous group of neoplasms with certain morphopathological patterns that may appear completely distant from the expected origin. Biphasic neoplasms with heterologous components representing transdifferentiation are uncommon and represent a challenge in diagnosis. Primary cutaneous melanomas with rhabdomyoblastic elements occur in humans but not in animals. We now describe a spontaneous case of cutaneous melanoma with rhabdomyosarcomatous component in a dog. A striated muscle heterologous component and dispersed cells with scarce melanin were interspersed in many regions of the neoplasm. The proportion of discrete melanocytic cells synthesizing scarce melanin was very low. The heterologous rhabdomyosarcomatous component contained well-differentiated striated muscle fibres, indicating that it was not rhabdoid. The muscle fibres were strongly immunoreactive for desmin, whereas the melanocytic component was diffusely, moderately immunopositive for Melan A, HMB45 and S100. To the author's knowledge, this is the first reported case of cutaneous melanoma with rhabdomyosarcomatous heterologous component in a dog.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"219 ","pages":"Pages 95-97"},"PeriodicalIF":0.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canine proliferative sparganosis: a case report and review of larval cestodiasis in dogs","authors":"Varvara Semenova ,&nbsp;Heather D.S. Walden ,&nbsp;Kelsey Bollenback ,&nbsp;James R. Cook Jr. ,&nbsp;Robert J. Ossiboff","doi":"10.1016/j.jcpa.2025.04.007","DOIUrl":"10.1016/j.jcpa.2025.04.007","url":null,"abstract":"<div><div>Larval cestodiasis is characterized by aberrant migration and proliferation of larval cestodes in body cavities or tissues and is a rarely reported entity in domestic dogs. Cestodes of the genera <em>Mesocestoides</em> and <em>Spirometra</em> are reported as causative agents, and can cause tetrathyridiosis and proliferative sparganosis, respectively. Reported herein is a case of abdominal larval cestodiasis in a companion dog from Florida, USA. During routine ovariohysterectomy, larval cestodes were noted throughout the peritoneum. Histologically, the larvae were characterized by asymmetric budding projections and contained tegument-lined cystic spaces. Polymerase chain reaction and Sanger sequencing of multiple gene targets, including <em>NAD</em>, <em>COX-1</em> and <em>mt12S</em>, identified the cestodes as a <em>Spirometra</em> sp with 100 % sequence identity to reference sequences from multiple species, including <em>Spirometra erinaceieuropaei</em>, <em>Spirometra ranarum</em> and <em>Spirometra mansoni</em>. While human proliferative sparganosis cases are routinely attributed to <em>Sparganum proliferum</em>, this report supports previous findings that <em>Spirometra</em> spp can cause proliferative sparganosis in dogs. <em>Spirometra</em> spp should be considered as a differential aetiology of proliferative sparganosis in dogs, and additional attention needs to be given to morphological and genetic characterization of <em>Spirometra</em> cestodes to better understand the role of these parasites in cases of animal sparganosis.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"219 ","pages":"Pages 48-51"},"PeriodicalIF":0.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}