Journal of Comparative Pathology最新文献

{"title":"Do we agree on counting mitotic figures in cytology of canine lymphoma? Three field selection methodologies by three observers","authors":"Filipe Sampaio,&nbsp;Carla Marrinhas,&nbsp;Ricardo Marcos,&nbsp;Mario Caniatti,&nbsp;Marta Santos","doi":"10.1016/j.jcpa.2026.07.004","DOIUrl":"10.1016/j.jcpa.2026.07.004","url":null,"abstract":"<div><div>Mitotic count is a key parameter of the cytological evaluation of canine lymphomas (CLs), but consensual guidelines for counting are missing. Variations in cell preservation are common in cytological smears and this can compromise the reproducibility of mitotic counting. We compared three methodologies for selecting fields for mitotic counting in CL cytological smears and interobserver agreement was assessed. Mitotic counting was performed in 19 CLs by three observers in five high-power fields (HPFs), selected according to the following methodologies: (A) randomly and with well-preserved cells; (B) adjacent fields within the best-preserved monolayer area; and (C) in the area with the highest mitotic activity (hot spots). Counts were analysed both as the total number in five HPFs and as number per mm²; using published cut-off values, mitotic counts were classified as low, intermediate or high. Counts by methods A and B were comparable, while consistently higher values were obtained with method C. Overall, the mitotic count classification shifted in 21% of the cases, with a higher assignment of cases to the high category using method C. Interobserver agreement was poor between observers using microscopes with different field of view diameters. When counts were normalized to number per mm², the agreement improved. The methodology of counting in adjacent fields showed better interobserver agreement compared to random fields. However, there was a high variability in the recorded numbers by each observer in highly proliferative CLs. The data highlights that mitotic counts in cytological smears of CL are highly dependent on methodology for selection of the fields and on the microscope.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"229 ","pages":"Pages 27-33"},"PeriodicalIF":1.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148678962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ‘Mitotic count and proliferative index in 24 cases of canine oral amelanotic melanoma: Tissue microarray cores and whole-section tissue analysis’ [J. Comp. Pathol. 222 (2025) 76, Abstract no. 449]","authors":"Kamila Bulak,&nbsp;Katarzyna Wankiewicz,&nbsp;Wojciech Łopuszyński","doi":"10.1016/j.jcpa.2026.06.006","DOIUrl":"10.1016/j.jcpa.2026.06.006","url":null,"abstract":"","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"229 ","pages":"Page 13"},"PeriodicalIF":1.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148646047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unilateral plantigrade stance in piglets associated with chronic neuritis following intramuscular medication","authors":"Alisson F. Silva,&nbsp;Vinicius Dahm,&nbsp;Amália Ferronato,&nbsp;Luana C.S. da Silva,&nbsp;Deise K. Alves da Silva,&nbsp;Ana P. Mori,&nbsp;Michelle P. Fochesatto,&nbsp;Paula R. de Almeida,&nbsp;Manoela M. Piva,&nbsp;Aline de M. Viott","doi":"10.1016/j.jcpa.2026.08.002","DOIUrl":"10.1016/j.jcpa.2026.08.002","url":null,"abstract":"<div><div>Locomotor disorders in pigs involve both infectious and non-infectious causes; however, iatrogenic peripheral neuropathies are rare. This study describes clinical, macroscopic and histological alterations associated with an outbreak of chronic neuritis in piglets following intramuscular drug administration on two farms. In two farms, each with 500 sows, approximately 7% of farrowing house piglets (26/370) presented with a plantigrade posture of the left pelvic limb (LPL) beginning on the third day of life, with no clinical improvement despite treatment. Nine piglets were subjected to necropsy. On gross examination, an off-white thickening involving the branches of the common fibular nerve of the LPL (indicative of fibrosis) was observed. On microscopic examination, Masson's trichrome and haematoxylin and eosin staining revealed granulomatous inflammation and abundant fibrovascular connective tissue between nerve fascicles, many of which were atrophied or undergoing degeneration. The anatomopathological findings were consistent with chronic (22.2%) and chronic-active (77.8%) neuritis and perineuritis associated with denervation-induced muscular atrophy, predominantly affecting the gastrocnemius muscle, which resulted in the plantigrade posture. Considering the location and pattern of the histological lesions, as well as the history of intramuscular injections during the first days of life, the lesions were considered to be of iatrogenic aetiology.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"229 ","pages":"Pages 44-49"},"PeriodicalIF":1.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148741996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary pancreatic histiocytic sarcoma with peritoneal sarcomatosis in a cat","authors":"Eduarda Keil,&nbsp;Augusto R. de Paula,&nbsp;Emanoelly M.S. da Silva,&nbsp;Adriane Strack,&nbsp;Ana C. Velasques,&nbsp;Welden Panziera,&nbsp;Marcele B. Bandinelli,&nbsp;Saulo P. Pavarini","doi":"10.1016/j.jcpa.2026.06.009","DOIUrl":"10.1016/j.jcpa.2026.06.009","url":null,"abstract":"<div><div>A 12-year-old neutered male Domestic Shorthair Cat, negative for feline immunodeficiency virus and feline leukaemia virus, was presented to the veterinary hospital with a 3-week history of anorexia, lethargy, abdominal pain and intermittent vomiting. Abdominal ultrasonography revealed a heterogeneous, poorly demarcated mass in the right mesogastric region, involving the pancreas and adjacent organs. Cytological evaluation of the abdominal fluid revealed a neoplastic effusion consistent with carcinomatosis. Given the poor prognosis, the cat was euthanized and submitted for necropsy, which revealed a large volume of free transudate within the abdominal cavity. In the pancreas, there was a 6.0 × 3.0 × 7.5 cm, irregular, soft, white-to-yellow mass and multiple small, firm, white nodules, ranging from 0.1 cm to 1 cm in diameter, were adhered to the omentum, mesentery, diaphragm and abdominal wall and to the serosal surfaces of the urinary bladder, intestines, spleen, stomach, liver and lungs. Microscopically, the pancreas contained a neoplastic proliferation composed predominantly of round cells arranged in loosely cohesive groups. Similar neoplastic proliferations were observed in the peritoneum, diaphragm, omentum and mesentery, the serosal surfaces of the urinary bladder, intestines, spleen, stomach, liver, left adrenal gland and lungs, and in the mediastinal lymph nodes. Based on these findings, the histopathological diagnosis was metastatic undifferentiated pancreatic neoplasia. Immunohistochemical evaluation revealed strong, diffuse cytoplasmic immunolabelling for vimentin and ionized calcium-binding adaptor molecule 1 in the neoplastic cells, with no labelling for pancytokeratin (AE1/AE3), E-cadherin, CD3 and CD20. The gross, histological and immunohistochemical findings were consistent with a histiocytic sarcoma of probable pancreatic origin, with metastatic dissemination throughout the peritoneum resembling that observed in primary pancreatic carcinomatosis. Histiocytic proliferative disorders are rare in cats, and the unusual presentation of this case provides an important contribution to the recognition of the possible manifestations of histiocytic sarcomas in this species.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"228 ","pages":"Pages 69-74"},"PeriodicalIF":1.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148411934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hidden in plain sight: searching for occult micrometastases and isolated tumour cells in feline mammary carcinomas","authors":"Joana Rodrigues-Jesus,&nbsp;João Urze-Almeida,&nbsp;Patrícia Dias-Pereira,&nbsp;Hugo Vilhena,&nbsp;Ana Canadas-Sousa,&nbsp;Augusto Faustino","doi":"10.1016/j.jcpa.2026.06.001","DOIUrl":"10.1016/j.jcpa.2026.06.001","url":null,"abstract":"<div><div>Regional lymph node status is a key prognostic factor in neoplastic lesions in cats and humans alike. The presence and clinical relevance of micrometastases and isolated tumour cells (ITCs) have been studied extensively in human breast cancer. However, comparable data in feline mammary carcinomas (FMCs) remain scarce; a single recent study addressed the presence of occult metastases. In this study, a multilevel sectioning protocol was applied to lymph nodes from 11 node-negative FMCs at first evaluation to search for occult metastases. The tissue in each paraffin-wax block was completely serially sectioned at 50 μm intervals, with one slide prepared at each tissue section and additional sections collected at every 150 μm interval for immunohistochemistry (IHC). ITCs were detected in two of the 11 cases on haematoxylin and eosin-stained tissue sections and confirmed by IHC for cytokeratin. No additional metastases were identified following IHC. These findings suggest that the assessment of multiple lymph node tissue levels may improve detection of metastases. Larger studies are necessary to determine the prevalence and clinical relevance of occult metastases in FMCs and their potential implications on treatment decisions.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"228 ","pages":"Pages 23-29"},"PeriodicalIF":1.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148277229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraskeletal osteosarcoma in two Guinea pigs (Cavia porcellus)","authors":"Crisan Smaniotto,&nbsp;Vinicius Dahm,&nbsp;Amália Ferronato,&nbsp;Karim Cristhine Pase Montagnini,&nbsp;Anaísa de Lima Grapiglia,&nbsp;Pietra Malu Franzener Detoni,&nbsp;Lorena dos Santos Pinheiro,&nbsp;Leonardo Gruchouskei,&nbsp;Anderson Luiz de Carvalho,&nbsp;Manoela Marchezan Piva,&nbsp;Aline de Marco Viott","doi":"10.1016/j.jcpa.2026.05.007","DOIUrl":"10.1016/j.jcpa.2026.05.007","url":null,"abstract":"<div><div>Osteosarcoma is a malignant osteogenic tumour of mesenchymal origin and is the most commonly reported neoplasm primarily affecting the appendicular skeleton in dogs and cats. When there is no involvement of bones, and the soft tissue is the primary site of the neoplasia, it is known as extraskeletal osteosarcoma (EOS). EOS is well documented in dogs and cats but is rarely reported in non-domestic species. Microscopically, an osteosarcoma is characterized by neoplastic proliferation of mesenchymal cells with production of osteoid matrix or immature bone. The aim of this study is to report two cases of EOS in guinea pigs (<em>Cavia porcellus</em>), as well as to identify and correlate clinical, radiographic, pathological and immunohistochemical findings. In case 1, multiple mesenteric and pancreatic nodules were present. Case 2 had a large subcutaneous mass with pulmonary and renal metastases. In both cases, radiographs revealed soft tissue masses with mineralized areas but no skeletal involvement. Histological examination confirmed malignant mesenchymal proliferation with osteoid deposition. Immunohistochemistry was applied, with the caveat that there are no specific immunohistochemical markers for definitive diagnosis of osteosarcoma. The neoplastic cells in both cases were strongly immunopositive for vimentin and RUNX2. The cases showed variable immunolabelling for S100 and alpha-smooth muscle actin, Ki-67 and p63. These cases enhance the clinicopathological spectrum of EOS in guinea pigs and highlight the importance of combining radiographic, histopathological and immunohistochemical evaluation for accurate diagnosis.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"228 ","pages":"Pages 30-36"},"PeriodicalIF":1.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148301884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathological findings in 61 free-ranging leopards (Panthera pardus) from the Greater Kruger National Park, South Africa. Part Two: non-parasitic conditions","authors":"Emily P. Mitchell,&nbsp;Lin-Mari de Klerk-Lorist,&nbsp;Peter Buss,&nbsp;Bjorn Reininghaus,&nbsp;Roy Bengis","doi":"10.1016/j.jcpa.2026.04.010","DOIUrl":"10.1016/j.jcpa.2026.04.010","url":null,"abstract":"<div><div>Few reports on diseases in free-ranging leopards (<em>Panthera pardus</em>) are available. A retrospective review of tissues from free-ranging leopards from the Greater Kruger National Park, South Africa, that were submitted for histological examination was conducted. Non-parasitic lesions were recorded in 61 free-ranging juvenile to elderly leopards. They had died or been euthanized between 1998 and 2023. Parasitic conditions in these leopards have been reported separately. Traumatic injury was common (41%). Respiratory tract conditions included pneumoconiosis (36%) and tuberculosis (23%), presumed to be due to <em>Mycobacterium bovis</em> infection in this endemic area. Classic granulomata were not present in the paucibacillary histiocytic lung lesions. Coronary arteriosclerosis, present in 36% of leopards, may be age-related, together with glomerulopathy (26%), adrenal gland corticomedullary fibrosis (8%), thyroid gland epithelial lipofuscinosis (n = 3), inactive ovaries (n = 2), obsolescent glomeruli (n = 1), pancreatic nodular hyperplasia (n = 1), bile duct carcinoma (n = 1) and cutaneous fibroma (n = 1). Mild tissue inflammation including interstitial nephritis (34%) and periportal hepatitis (28%) was often recorded. Tuberculosis and rabies were the only diagnosed infectious diseases recorded in this series of leopards, 85% of which were euthanized due mainly to the perceived risk they posed to humans rather than clinical illness. Apart from these diseases and trauma, most of the lesions noted in these animals were likely incidental in free-ranging animals. Lack of information on population dynamics and disease status in the free-ranging leopard population means that the true prevalence of these conditions is not known.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"228 ","pages":"Pages 45-52"},"PeriodicalIF":1.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148330019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and immunohistochemical features of oral telangiectatic osteosarcomas in dogs and their differentiation from oral haemangiosarcomas","authors":"Daniel R. Rissi,&nbsp;Ricardo E. Mendes","doi":"10.1016/j.jcpa.2026.06.005","DOIUrl":"10.1016/j.jcpa.2026.06.005","url":null,"abstract":"<div><div>Oral osteosarcoma accounts for a significant proportion of axial osteosarcomas. Telangiectatic osteosarcoma is a rare and aggressive osteosarcoma subtype that consists of blood-filled spaces that may be difficult to differentiate from aneurysmal bone cysts and haemangiosarcomas. Here we describe the clinical, histological and immunohistochemical features of nine oral telangiectatic osteosarcomas and four oral haemangiosarcomas in dogs. Medical records and histological samples were reviewed and immunohistochemistry (IHC) for RUNX2 and CD31 was performed on all cases. Oral telangiectatic osteosarcomas occurred in five castrated male dogs, three spayed female dogs and one female dog of different breeds with a median age of 11 years. Tumours consisted of rapidly growing, ulcerated and occasionally haemorrhagic oral masses involving the maxilla, mandible or gingiva. Histologically, neoplasms were infiltrative and composed of pleomorphic neoplastic cells forming blood-filled spaces admixed with solid bundles and irregular osteoid. All cases had strong nuclear RUNX2 immunolabelling and lacked CD31 immunolabelling. Haemangiosarcomas occurred in four spayed female dogs of multiple breeds with a median age of 9.4 years. Clinical signs consisted of oral bleeding from red and/or ulcerated tumours arising from the right maxilla, left mandible and right mandible. Haemangiosarcomas were composed of vascular channels lined by CD31-positive endothelial cells, with no osteoid or RUNX2 labelling. Oral telangiectatic osteosarcoma is a rare osteosarcoma subtype with histological features that resemble those of haemangiosarcomas, which can complicate the diagnosis, particularly in small biopsies. The combined use of histology and IHC (RUNX2 and CD31) supports reliable differentiation between these neoplasms.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"228 ","pages":"Pages 92-97"},"PeriodicalIF":1.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148470963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical characterization of immune cell and metabolic markers in canine prostate carcinoma","authors":"Taiki Kato,&nbsp;Masashi Sakurai,&nbsp;Masanao Ichimata,&nbsp;Yumiko Kagawa,&nbsp;Hiroto Toyoda,&nbsp;Ryuzo Katayama,&nbsp;Kei Harada,&nbsp;Eri Fukazawa,&nbsp;Fukiko Matsuyama,&nbsp;Tetsuya Kobayashi,&nbsp;Takuya Mizuno,&nbsp;Masaya Igase","doi":"10.1016/j.jcpa.2026.05.005","DOIUrl":"10.1016/j.jcpa.2026.05.005","url":null,"abstract":"<div><div>Canine prostate carcinoma (cPCA) is an aggressive malignancy with limited prognostic markers. This study aimed to comprehensively characterize immune-cell infiltration, glycolysis, hypoxia and oncogenic markers in cPCA and assess their clinical relevance. Tumour tissues from 26 dogs with cPCA and hyperplastic prostate tissues from seven dogs were analysed using immunohistochemistry. Five immune cell markers (CD3, CD20, CD8, Foxp3 and CD204) were quantitatively assessed, and the amounts of immunolabelling of monocarboxylate transporter 4 (MCT4), glucose transporter 1 (GLUT1), human epidermal growth factor receptor 2 and Ki-67 were evaluated through established scoring methods. Associations between progression-free survival and overall survival were analysed using univariate Cox regression. All tumours were infiltrated by multiple immune-cell subsets, characterized by marked intertumoral heterogeneity. Immune-cell densities were generally higher in cPCA than in hyperplastic prostate tissue. Compared with hyperplastic prostate tissue, cPCA showed lower MCT4 and higher GLUT1 expression. No significant associations were observed between immune-cell density or marker expression and clinical outcomes. The findings suggest that cPCA has widespread immune-cell infiltration and distinct metabolic features; however, individual immunohistochemical markers alone were insufficient to predict clinical outcomes.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"228 ","pages":"Pages 56-62"},"PeriodicalIF":1.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148338608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral polyneuropathy in three puppies temporally associated with canine parvovirus infection resembling acute polyradiculoneuritis","authors":"Carmen Manuela Stan,&nbsp;Clare Rusbridge,&nbsp;Fabio Stabile,&nbsp;Guido Rocchigiani,&nbsp;G. Diane Shelton,&nbsp;Susan Halstead,&nbsp;Ana Maria Coelho,&nbsp;Jessica Bacon,&nbsp;Mark Lowrie","doi":"10.1016/j.jcpa.2026.06.004","DOIUrl":"10.1016/j.jcpa.2026.06.004","url":null,"abstract":"<div><div>Molecular mimicry is hypothesized to play a key role in the pathogenesis of acute polyradiculoneuritis (APRN) in dogs, as in Guillain-Barré syndrome (GBS). GBS is linked to <em>Campylobacter jejuni</em> and cytomegalovirus, while <em>Parvovirus B19</em> shows temporal and serological associations. In dogs, the cause of APRN is often unknown, although recent vaccination or ingestion of raw poultry contaminated with <em>Clostridium perfringens</em> is documented. Antiganglioside antibodies are potential serum biomarkers for APRN.</div><div>Eight 2.5-month-old Sprocker littermates and a 3-month-old female Cockapoo developed severe gastrointestinal clinical signs and tested positive for canine parvovirus-2 (CPV-2) infection. Six littermates died, while two pups (one male, one female) and the Cockapoo recovered. Seven days later, the male puppy developed pelvic limb weakness progressing to flaccid tetraparesis over 24 h, followed by thoracic limb contracture. Both female pups, 10 days after recovering from illness, developed neuromuscular clinical signs that deteriorated for 9 days before improving. They presented with a short-strided, choppy gait with kyphosis, and the Cockapoo developed dysphonia. Both female puppies improved over several months. Antiganglioside antibodies were not detected in the two littermates at 1 and 3 months after clinical onset. Treatment included supportive care. Both littermates received prednisolone and methocarbamol, with only the female responding. The male was euthanized due to poor quality of life. Post-mortem examination revealed widespread muscular atrophy and axonal degeneration, with active regeneration detected on semi-thin tissue sections of nerves.</div><div>This study described a peripheral neuropathy temporally associated with CPV-2 infection. Although a causal relationship cannot be established, the clinical course and pathological features are compatible with a post-infectious neuromuscular process.</div></div>","PeriodicalId":15520,"journal":{"name":"Journal of Comparative Pathology","volume":"228 ","pages":"Pages 63-68"},"PeriodicalIF":1.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}