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Journal of chromatography最新文献

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Cumulative author, subject, compound and special issue indexes. Volumes 601-650 (1992-1993). 累积的作者、主题、复合和专刊索引。第601-650卷(1992-1993)。
Journal of chromatography Pub Date : 1994-05-20
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引用次数: 0
Processing of envelope polypeptides of herpes simplex virus type 1. Demonstration of variation in different cell lines by high-performance liquid chromatography and radioimmunoprecipitation. 1型单纯疱疹病毒包膜多肽的处理。用高效液相色谱法和放射免疫沉淀法证明不同细胞系的差异。
Journal of chromatography Pub Date : 1994-02-11
G Kessie, D M Dela Cruz, M A Taha, F J al-Shammary, A F Tawfik, M N al-Ahdal
{"title":"Processing of envelope polypeptides of herpes simplex virus type 1. Demonstration of variation in different cell lines by high-performance liquid chromatography and radioimmunoprecipitation.","authors":"G Kessie,&nbsp;D M Dela Cruz,&nbsp;M A Taha,&nbsp;F J al-Shammary,&nbsp;A F Tawfik,&nbsp;M N al-Ahdal","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>[35S]Methionine-labelled envelope polypeptides of herpes simplex virus type 1, strain F, propagated in mammalian cell culture of various origins, were separated by ion-exchange high-performance liquid chromatography on a TSK DEAE-3SW column. Analysis of the fractions by radioimmunoprecipitation followed by sodium dodecyl sulphate polyacrylamide gel electrophoresis of the immunoprecipitates showed similarities as well as distinct differences in the number, migration patterns and molecular mass of the synthesized polypeptides, depending on the host cell. The results show that this method can be used to demonstrate species-specific or organ-specific differences in the processing of virus-specified polypeptides synthesized in host cells.</p>","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"652 2","pages":"234-9"},"PeriodicalIF":0.0,"publicationDate":"1994-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19000969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assay of urinary 2,3-dinor-6-oxo prostaglandin F1 alpha by gas chromatography-tandem mass spectrometry. 气相色谱-串联质谱法测定尿2,3-二-6-氧前列腺素F1 α。
Journal of chromatography Pub Date : 1993-12-22
A Ferretti, V P Flanagan
{"title":"Assay of urinary 2,3-dinor-6-oxo prostaglandin F1 alpha by gas chromatography-tandem mass spectrometry.","authors":"A Ferretti,&nbsp;V P Flanagan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Prostacyclin (PGI2), an important determinant of cardiovascular biology, is produced from arachidonic acid by endothelial cells. Measurement of its stable urinary metabolite, 2,3-dinor-6-oxo prostaglandin F1 alpha (PGI2-M), is the approach of choice to assess variations of the endogenous synthesis of PGI2 that occur in response to dietary, pharmacological and pathological alterations. We developed a relatively simple stable isotope dilution assay for PGI2-M which involves solid-phase extraction of 10 ml of urine with Chem Elut disposable columns, water/solvent partitioning from basic and acidic environments with ethyl acetate and methylene chloride, derivatization to 1-pentafluorobenzyl ester followed by TLC, methoximation and trimethylsilylation. Quantification was achieved, for the first time for PGI2-M, by capillary GC-electron capture negative ion MS-MS with a triple quadrupole mass spectrometer operated in the negative ion detection mode with methane as moderating gas. The mean inter-assay R.S.D., determined on 12 different urine samples, was 5.1 (range 0.4% to 10.5%). The excretion of PGI2-M in 34 healthy male subjects (age 26 to 57) was 156.2 +/- 65.2 (mean +/- S.D.) ng/24 h.</p>","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"622 2","pages":"109-15"},"PeriodicalIF":0.0,"publicationDate":"1993-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19141627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Simultaneous analysis of homovanillic acid, 5-hydroxyindoleacetic acid, 3-methoxy-4-hydroxyphenylethylene glycol and vanilmandelic acid in plasma from alcoholics by high-performance liquid chromatography with electrochemical detection. Critical comparison of solid-phase and liquid-liquid extraction methods. 高效液相色谱-电化学检测法同时分析酒精类饮料血浆中香草酸、5-羟基吲哚乙酸、3-甲氧基-4-羟基苯基乙二醇和香草酸。固相萃取法与液-液萃取法的关键性比较。
Journal of chromatography Pub Date : 1993-12-22
J Hartleb, S Eue, A Kemper
{"title":"Simultaneous analysis of homovanillic acid, 5-hydroxyindoleacetic acid, 3-methoxy-4-hydroxyphenylethylene glycol and vanilmandelic acid in plasma from alcoholics by high-performance liquid chromatography with electrochemical detection. Critical comparison of solid-phase and liquid-liquid extraction methods.","authors":"J Hartleb,&nbsp;S Eue,&nbsp;A Kemper","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method is described for the simultaneous determination of vanilmandelic acid, 3-methoxy-4-hydroxyphenylethylene glycol, 5-hydroxyindoleacetic acid, and homovanillic acid in a human plasma sample using reversed-phase high-performance liquid chromatography with column switching and amperometric detection. Two methods of sample preparation were tested. Liquid-liquid extraction yields better recoveries, is more selective and precise than solid-phase extraction and allows a shorter time of chromatographic analysis. Estimated plasma values of the metabolites from healthy controls are in good agreement with previously reported levels. Studies of alcoholics at the beginning of the delirium tremens provided different plasma levels of the metabolites, dependent on the different duration--and hence the severity--of the delirium.</p>","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"622 2","pages":"161-71"},"PeriodicalIF":0.0,"publicationDate":"1993-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18521982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of the aza alkyl lysophospholipid 3-methoxy-2-N,N-methyloctadecylaminopropyloxyphosphorylcholine in rat plasma by liquid chromatography-particle beam-mass spectrometry. 液相色谱-粒子束质谱法测定大鼠血浆中偶氮烷基溶血磷脂3-甲氧基-2- n, n -甲基locloctadecylaminopropyloxyphospphospylcholine。
Journal of chromatography Pub Date : 1993-12-22
C Celma
{"title":"Determination of the aza alkyl lysophospholipid 3-methoxy-2-N,N-methyloctadecylaminopropyloxyphosphorylcholine in rat plasma by liquid chromatography-particle beam-mass spectrometry.","authors":"C Celma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A sensitive and specific method for the determination of the aza alkyl lysophospholipid (AALP) 3-methoxy-2-N,N-methyloctadecylaminopropyloxyphosphorylcholine (I) in rat plasma is described. The target molecule was analyzed by high-performance liquid chromatography (HPLC)-mass spectrometry (MS) after one single liquid-liquid extraction with chloroform-methanol (2:1, v/v). 1,2-Didecanoyl-sn-glycero-3-phosphocholine was used as internal standard. HPLC was carried out using a polymeric reversed-phase column; the coupling to the mass spectrometer was a particle beam (PB) interface, and the ionization method was electron impact (EI). This simple and rugged method permits the measurement of I in rat plasma in the range of 25 ng/ml-5 micrograms/ml with good accuracy and precision and is used in pharmacokinetic studies.</p>","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"622 2","pages":"215-22"},"PeriodicalIF":0.0,"publicationDate":"1993-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19142264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Direct determination of ephedrine and norephedrine in human urine by capillary zone electrophoresis. 毛细管区带电泳法直接测定人尿中的麻黄碱和去甲麻黄碱。
Journal of chromatography Pub Date : 1993-12-08
M Chicharro, A Zapardiel, E Bermejo, J A Perez, L Hernández
{"title":"Direct determination of ephedrine and norephedrine in human urine by capillary zone electrophoresis.","authors":"M Chicharro,&nbsp;A Zapardiel,&nbsp;E Bermejo,&nbsp;J A Perez,&nbsp;L Hernández","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This paper describes a simple and fast method for the simultaneous determination of ephedrine and norephedrine in urine by capillary zone electrophoresis. Separation and determination of these stimulants in human urine was performed in 50 mM phosphate buffer at pH 9.5, modifying the electroosmotic flow with acetonitrile. The method allows direct determination of the stimulants in urine in concentrations lower than 20.0 micrograms/ml, and has a limit of determination of 2.6 +/- 0.2 micrograms/ml for ephedrine and 2.3 +/- 0.2 micrograms/ml for norephedrine in urine and may be applied for doping control.</p>","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"622 1","pages":"103-8"},"PeriodicalIF":0.0,"publicationDate":"1993-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19112349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of radiolabeled metabolites of nicotine in rat bile. Synthesis of S-(-)-nicotine N-glucuronide and direct separation of nicotine-derived conjugates using high-performance liquid chromatography. 大鼠胆汁中尼古丁放射性代谢物的鉴定。S-(-)-尼古丁n -葡萄糖醛酸盐的合成及尼古丁衍生物的高效液相色谱直接分离。
Journal of chromatography Pub Date : 1993-11-17
M J Seaton, E S Vesell, H Luo, E M Hawes
{"title":"Identification of radiolabeled metabolites of nicotine in rat bile. Synthesis of S-(-)-nicotine N-glucuronide and direct separation of nicotine-derived conjugates using high-performance liquid chromatography.","authors":"M J Seaton,&nbsp;E S Vesell,&nbsp;H Luo,&nbsp;E M Hawes","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Four metabolites of nicotine, including two glucuronides, have been separated by high-performance liquid chromatography. This separation was applied to identification of biliary metabolites of radiolabeled nicotine by radiometric detection. S-(-)-Nicotine N-glucuronide was synthesized and used as a standard in method development.</p>","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"621 1","pages":"49-53"},"PeriodicalIF":0.0,"publicationDate":"1993-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19294783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Automated sample preparation by on-line dialysis and trace enrichment. Analysis of morphine, 6-monoacetylmorphine, codeine, ethylmorphine and pholcodine in plasma and whole blood by capillary gas chromatography and capillary gas chromatography-mass spectrometry. 通过在线透析和微量富集自动样品制备。血浆和全血中吗啡、6-单乙酰吗啡、可待因、乙基吗啡和福可定的毛细管气相色谱和毛细管气相色谱-质谱分析。
Journal of chromatography Pub Date : 1993-11-17
M Krogh, A S Christophersen, K E Rasmussen
{"title":"Automated sample preparation by on-line dialysis and trace enrichment. Analysis of morphine, 6-monoacetylmorphine, codeine, ethylmorphine and pholcodine in plasma and whole blood by capillary gas chromatography and capillary gas chromatography-mass spectrometry.","authors":"M Krogh,&nbsp;A S Christophersen,&nbsp;K E Rasmussen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A fully automated sample preparation method for the determination of five opiates in human plasma and whole blood is described. The technique combines dialysis and trace enrichment prior to gas chromatography and gas chromatography-mass spectrometry. Dialysis and trace enrichment on a polymer column was shown to be a highly reliable method for sample preparation. The method can be used, after minor modification, to determine other basic drugs in plasma and whole blood. The method demonstrates the potential of the automated sequential trace enrichment of dialysate (ASTED) system for automated sample preparation.</p>","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"621 1","pages":"41-8"},"PeriodicalIF":0.0,"publicationDate":"1993-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19294782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Liquid chromatographic determination of para-substituted N,N-dialkylaniline N-oxides. 液相色谱法测定对取代N,N-二烷基苯胺N-氧化物。
Journal of chromatography Pub Date : 1993-09-08
Y Seto, F P Guengerich
{"title":"Liquid chromatographic determination of para-substituted N,N-dialkylaniline N-oxides.","authors":"Y Seto,&nbsp;F P Guengerich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A high-performance liquid chromatographic method for the determination of p-substituted N,N-dialkylaniline N-oxides [N,N-dimethylaniline N-oxide (DMANO), N,N-dimethyl-p-toluidine N-oxide, N,N-diethylaniline N-oxide, N-ethyl-N-methylaniline N-oxide (EMANO), p-cyano-N,N-dimethylaniline N-oxide (pCNDMANO), and N-phenylpyrrolidine N-oxide (PPNO)] has been developed. It uses an octadecylsilica column, a mobile phase of methanol-phosphate buffer (pH 7.0, adjusted by triethylamine) and ultraviolet detection. N-Oxides were eluted in the order of hydrophilicity except for PPNO, which eluted between DMANO and EMANO. The number of theoretical plates and the detection limit under optimized conditions were between 2400 (DMANO) and 5400 (pCNDMANO) and between 2.3 microM (pCNDMANO) and 30 microM (PPNO), respectively. The concentration of N-oxides recovered from enzyme reaction mixtures by solid-phase extraction using Sep-Pak C18 (prior to chromatography) was also optimized with regard to the sensitivity and interference.</p>","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"619 1","pages":"71-7"},"PeriodicalIF":0.0,"publicationDate":"1993-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19233744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methionine enkephalin-like immunoreactivity, substance P-like immunoreactivity and beta-endorphin-like immunoreactivity post-mortem stability in rat pituitary. 蛋氨酸脑啡肽样免疫反应性、p物质样免疫反应性和-内啡肽样免疫反应性对大鼠垂体死后稳定性的影响。
Journal of chromatography Pub Date : 1993-07-02
X Zhu, D M Desiderio
{"title":"Methionine enkephalin-like immunoreactivity, substance P-like immunoreactivity and beta-endorphin-like immunoreactivity post-mortem stability in rat pituitary.","authors":"X Zhu,&nbsp;D M Desiderio","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Significant post-mortem changes in peptide concentration occur within the previously unstudied timeframe, i.e. within 1 h, for the proenkephalin A, proopiomelanoccortin, and tachykinin neuropeptidergic systems in the pituitary. These data differ from data obtained in other studies that concluded that peptides are stable for up to 72 h post-mortem. The post-mortem stability of the three neuropeptides, methionine enkephalin, substance P, and beta-endorphin, was studied in the rat pituitary to test the hypothesis that significant post-mortem concentration changes of those three neuropeptides occur in the immediate post-mortem time period.</p>","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"616 2","pages":"175-87"},"PeriodicalIF":0.0,"publicationDate":"1993-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18693979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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