{"title":"液相色谱-粒子束质谱法测定大鼠血浆中偶氮烷基溶血磷脂3-甲氧基-2- n, n -甲基locloctadecylaminopropyloxyphospphospylcholine。","authors":"C Celma","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A sensitive and specific method for the determination of the aza alkyl lysophospholipid (AALP) 3-methoxy-2-N,N-methyloctadecylaminopropyloxyphosphorylcholine (I) in rat plasma is described. The target molecule was analyzed by high-performance liquid chromatography (HPLC)-mass spectrometry (MS) after one single liquid-liquid extraction with chloroform-methanol (2:1, v/v). 1,2-Didecanoyl-sn-glycero-3-phosphocholine was used as internal standard. HPLC was carried out using a polymeric reversed-phase column; the coupling to the mass spectrometer was a particle beam (PB) interface, and the ionization method was electron impact (EI). This simple and rugged method permits the measurement of I in rat plasma in the range of 25 ng/ml-5 micrograms/ml with good accuracy and precision and is used in pharmacokinetic studies.</p>","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"622 2","pages":"215-22"},"PeriodicalIF":0.0000,"publicationDate":"1993-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Determination of the aza alkyl lysophospholipid 3-methoxy-2-N,N-methyloctadecylaminopropyloxyphosphorylcholine in rat plasma by liquid chromatography-particle beam-mass spectrometry.\",\"authors\":\"C Celma\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A sensitive and specific method for the determination of the aza alkyl lysophospholipid (AALP) 3-methoxy-2-N,N-methyloctadecylaminopropyloxyphosphorylcholine (I) in rat plasma is described. The target molecule was analyzed by high-performance liquid chromatography (HPLC)-mass spectrometry (MS) after one single liquid-liquid extraction with chloroform-methanol (2:1, v/v). 1,2-Didecanoyl-sn-glycero-3-phosphocholine was used as internal standard. HPLC was carried out using a polymeric reversed-phase column; the coupling to the mass spectrometer was a particle beam (PB) interface, and the ionization method was electron impact (EI). This simple and rugged method permits the measurement of I in rat plasma in the range of 25 ng/ml-5 micrograms/ml with good accuracy and precision and is used in pharmacokinetic studies.</p>\",\"PeriodicalId\":15508,\"journal\":{\"name\":\"Journal of chromatography\",\"volume\":\"622 2\",\"pages\":\"215-22\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1993-12-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of chromatography\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of chromatography","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
建立了测定大鼠血浆中3-甲氧基-2- n, n -甲基locloctadecylaminopropyloxopylcholine (I)的灵敏、特异方法。目的分子经氯仿-甲醇(2:1,v/v)单液-液萃取后，采用高效液相色谱-质谱法(MS)分析。1,2-二癸醇-sn-甘油-3-磷酸胆碱作为内标。HPLC采用聚合物反相色谱柱;与质谱仪的耦合方式为粒子束(PB)界面，电离方式为电子冲击(EI)。该方法简单可靠，可测量大鼠血浆中I含量，范围为25 ng/ml-5微克/ml，准确度和精密度高，可用于药代动力学研究。
Determination of the aza alkyl lysophospholipid 3-methoxy-2-N,N-methyloctadecylaminopropyloxyphosphorylcholine in rat plasma by liquid chromatography-particle beam-mass spectrometry.
A sensitive and specific method for the determination of the aza alkyl lysophospholipid (AALP) 3-methoxy-2-N,N-methyloctadecylaminopropyloxyphosphorylcholine (I) in rat plasma is described. The target molecule was analyzed by high-performance liquid chromatography (HPLC)-mass spectrometry (MS) after one single liquid-liquid extraction with chloroform-methanol (2:1, v/v). 1,2-Didecanoyl-sn-glycero-3-phosphocholine was used as internal standard. HPLC was carried out using a polymeric reversed-phase column; the coupling to the mass spectrometer was a particle beam (PB) interface, and the ionization method was electron impact (EI). This simple and rugged method permits the measurement of I in rat plasma in the range of 25 ng/ml-5 micrograms/ml with good accuracy and precision and is used in pharmacokinetic studies.
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