Determination of the aza alkyl lysophospholipid 3-methoxy-2-N,N-methyloctadecylaminopropyloxyphosphorylcholine in rat plasma by liquid chromatography-particle beam-mass spectrometry.
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引用次数: 0
Abstract
A sensitive and specific method for the determination of the aza alkyl lysophospholipid (AALP) 3-methoxy-2-N,N-methyloctadecylaminopropyloxyphosphorylcholine (I) in rat plasma is described. The target molecule was analyzed by high-performance liquid chromatography (HPLC)-mass spectrometry (MS) after one single liquid-liquid extraction with chloroform-methanol (2:1, v/v). 1,2-Didecanoyl-sn-glycero-3-phosphocholine was used as internal standard. HPLC was carried out using a polymeric reversed-phase column; the coupling to the mass spectrometer was a particle beam (PB) interface, and the ionization method was electron impact (EI). This simple and rugged method permits the measurement of I in rat plasma in the range of 25 ng/ml-5 micrograms/ml with good accuracy and precision and is used in pharmacokinetic studies.
液相色谱-粒子束质谱法测定大鼠血浆中偶氮烷基溶血磷脂3-甲氧基-2- n, n -甲基locloctadecylaminopropyloxyphospphospylcholine。
建立了测定大鼠血浆中3-甲氧基-2- n, n -甲基locloctadecylaminopropyloxopylcholine (I)的灵敏、特异方法。目的分子经氯仿-甲醇(2:1,v/v)单液-液萃取后，采用高效液相色谱-质谱法(MS)分析。1,2-二癸醇-sn-甘油-3-磷酸胆碱作为内标。HPLC采用聚合物反相色谱柱;与质谱仪的耦合方式为粒子束(PB)界面，电离方式为电子冲击(EI)。该方法简单可靠，可测量大鼠血浆中I含量，范围为25 ng/ml-5微克/ml，准确度和精密度高，可用于药代动力学研究。
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