Journal of Chromatography B: Biomedical Sciences and Applications最新文献_第3页

High performance liquid chromatographic analysis of St. John’s Wort with photodiode array detection 光电二极管阵列检测圣约翰草的高效液相色谱分析

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00404-2

Wenkui Li, John F Fitzloff

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引用次数: 59

Determination of LSD and its metabolites in human biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry 高效液相色谱-电喷雾串联质谱法测定人体液中LSD及其代谢物

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00386-3

J Canezin , A Cailleux , A Turcant , A Le Bouil , P Harry , P Allain

{"title":"Determination of LSD and its metabolites in human biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry","authors":"J Canezin , A Cailleux , A Turcant , A Le Bouil , P Harry , P Allain","doi":"10.1016/S0378-4347(01)00386-3","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00386-3","url":null,"abstract":"<div>A liquid chromatographic procedure with electrospray ionization tandem mass spectrometric detection has been developed and validated for LSD and iso-LSD determination. A one-step liquid–liquid extraction on 1 ml blood or urine was used. The lower limit for quantitative determination was 0.02 μg/l for LSD and iso-LSD. The analytical procedure has been applied in two positive cases (case 1: LSD=0.31 μg/l, iso-LSD=0.27 μg/l in plasma and LSD=1.30 μg/l, iso-LSD=0.82 μg/l in urine; case 2: LSD=0.24 μg/l, iso-LSD=0.6 μg/l in urine). LSD metabolism was investigated using MS–MS neutral loss monitoring for the screening of potential metabolites. The main metabolite was 2-oxo-3-hydroxy-LSD (O–H–LSD) present in urine at the concentrations of 2.5 μg/l and 6.6 μg/l, respectively, for case 1 and 2, and was not present in plasma. Nor-LSD was also found in urine at 0.15 and 0.01 μg/l levels. Nor-iso-LSD, lysergic acid ethylamide (LAE), trioxylated-LSD, lysergic acid ethyl-2-hydroxyethylamide (LEO) and 13 and 14-hydroxy-LSD and their glucuronide conjugates were detected in urine using specific MS–MS transitions.</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 15-27"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00386-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 53

Direct chiral assay of tramadol and detection of the phase II metabolite O-demethyl-tramadol glucuronide in human urine using capillary electrophoresis with laser-induced native fluorescence detection 曲马多直接手性测定及尿中II期代谢物o -去甲基曲马多葡糖苷的毛细管电泳激光诱导荧光检测

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00366-8

Uta Birgitta Soetebeer , Marc-Oliver Schierenberg , Harald Schulz , Peter Andresen , Gottfried Blaschke

{"title":"Direct chiral assay of tramadol and detection of the phase II metabolite O-demethyl-tramadol glucuronide in human urine using capillary electrophoresis with laser-induced native fluorescence detection","authors":"Uta Birgitta Soetebeer , Marc-Oliver Schierenberg , Harald Schulz , Peter Andresen , Gottfried Blaschke","doi":"10.1016/S0378-4347(01)00366-8","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00366-8","url":null,"abstract":"<div>A chiral separation using carboxymethyl-β-cyclodextrin and methyl-β-cyclodextrin for the direct assay of tramadol in human urine by capillary electrophoresis (CE) with laser-induced native fluorescence detection was developed. Furthermore, the phase II metabolite O-demethyl tramadol glucuronide was determined from the urine samples and the ratio of the diasteromers was determined. The chiral method was validated. Correlation coefficients were higher than 0.999. Within day variation showed accuracy in the range 96.1–105.8% with a RSD less than 6.00%. Day to day variation present an accuracy ranging from 100.2 to 103.5% with a RSD less than 5.4%. After oral administration of 150 mg tramadol hydrochloride to a healthy volunteer, the urinary excretion was monitored during 24 h. About 11.4% of the dose was excreted as 1S,2S-tramadol, 16.4% as 1R,2R-tramadol and 23.7% as O-demethyl tramadol glucuronide. The amount of 1S,2S O-demethyl tramadol glucuronide was more than three fold higher as 1R,2R-O-demethyl tramadol glucuronide. The enantiomeric ratio of tramadol and the diastereomeric ratio of O-demethyl tramadol glucuronide was deviated from 1.0 showing that a stereoselective metabolism of tramadol occurs.</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 3-13"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00366-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Determination of hyperforin, hypericin, and pseudohypericin in human plasma using high-performance liquid chromatography analysis with fluorescence and ultraviolet detection 荧光和紫外检测高效液相色谱法测定人血浆中的金丝桃素、金丝桃素和假金丝桃素

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00390-5

Steffen Bauer , Elke Störmer , Hans-Joachim Graubaum , Ivar Roots

引用次数: 42

Novel and simple two-step purification of a full-length rat glucocorticoid-receptor expressed in a baculovirus system. 杆状病毒系统中表达的全长大鼠糖皮质激素受体的新颖和简单的两步纯化。

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00408-X

M. Hyodo, K. Okamoto, K. Shibata, N. Suematsu, F. Isohashi

引用次数: 3

Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography. 高效液相色谱法测定鲍鱼组织中磷酸甘氨酸的优化方法。

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00428-5

M. Viant, E. Rosenblum, R. Tjeerdema

引用次数: 42

Metabolism of methandrostenolone in the horse: a gas chromatographic–mass spectrometric investigation of phase I and phase II metabolism 马体内甲雄甾酮的代谢:第一期和第二期代谢的气相色谱-质谱研究

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00409-1

Andrew R McKinney , Damon D Ridley , Craig J Suann

引用次数: 15

Quantitative analysis of fentanyl in rat plasma by gas chromatography with nitrogen-phosphorus detection. 大鼠血浆中芬太尼的氮磷气相色谱定量分析。

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00405-4

H. Choi, H. C. Shin, G. Khang, J. M. Rhee, H. B. Lee

引用次数: 28

Determination of LSD and its metabolites in human biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry. 高效液相色谱-电喷雾串联质谱法测定人体液中LSD及其代谢物。

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00386-3

J. Canezin, A. Cailleux, A. Turcant, A. Bouil, P. Harry, Pierre Allain

引用次数: 52

Direct chiral assay of tramadol and detection of the phase II metabolite O-demethyl tramadol glucuronide in human urine using capillary electrophoresis with laser-induced native fluorescence detection. 曲马多直接手性测定及尿中II期代谢物o -去甲基曲马多葡糖苷的毛细管电泳激光诱导荧光检测。

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00366-8

U. B. Soetebeer, M. Schierenberg, H. Schulz, P. Andresen, G. Blaschke

引用次数: 30