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Stereoselective analysis of fluvastatin in human plasma for pharmacokinetic studies. 立体选择分析氟伐他汀在人血浆中的药代动力学研究。
Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00407-8
V. Lanchote, A. Rocha, F. U. de Albuquerque, E. Coelho, P. S. Bonato
{"title":"Stereoselective analysis of fluvastatin in human plasma for pharmacokinetic studies.","authors":"V. Lanchote, A. Rocha, F. U. de Albuquerque, E. Coelho, P. S. Bonato","doi":"10.1016/S0378-4347(01)00407-8","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00407-8","url":null,"abstract":"","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"20 1","pages":"81-8"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87877114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 23
Liquid chromatography–mass spectrometry assay of a thiadiazole derivative in mice: application to pharmacokinetic studies 小鼠体内噻二唑衍生物的液相色谱-质谱分析:在药代动力学研究中的应用
Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 Epub Date: 2001-10-02 DOI: 10.1016/S0378-4347(01)00403-0
Hong Wong , Lee Jia , Jim B Camden , Steve D Weitman
{"title":"Liquid chromatography–mass spectrometry assay of a thiadiazole derivative in mice: application to pharmacokinetic studies","authors":"Hong Wong ,&nbsp;Lee Jia ,&nbsp;Jim B Camden ,&nbsp;Steve D Weitman","doi":"10.1016/S0378-4347(01)00403-0","DOIUrl":"10.1016/S0378-4347(01)00403-0","url":null,"abstract":"<div><p>Modern atmospheric pressure ionization (API) ion-trap mass spectrometry in connection with fast chromatographic separations using a short narrow-bore C<sub>8</sub> column was developed to determine 5-phenyl-3-thioureido-1,2,4-thiadiazole (301029), a novel virus inhibitor in serum. Both 301029 and an internal standard (I.S.) were separated from serum samples by acetonitrile deproteinization and extraction without time-consuming reconstitution. The chromatographic separation was achieved on a C<sub>8</sub> reversed-phase narrow-bore column using acetonitrile–water–acetic acid (90:10:0.01, v/v/v) as a mobile phase. The mass spectrometric analysis was performed by atmospheric pressure chemical ionization (APCI) mode with positive ion detection. Single ion monitoring (SIM) scan mode of <em>m/z</em> 237 and 158 was used to quantitatively determine 301029 and I.S., respectively. The low limit of quantitation was 25 ng/ml. The assay exhibited a linear range of 25–2500 ng/ml. Recovery from serum proved to be 100–113%. The precision (C.V.) and accuracy (RE) of the method were 2–12% and 94–112%, respectively. The present method was applied to determine the pharmacokinetic parameters of 301029 following oral administration of the agent to mice at 5 g/kg. The results revealed that the elimination half-life of 301029 was 413 min and the area under serum concentration–time curve was 354 μg/ml/min.</p></div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 55-62"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00403-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76462318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography. 高效液相色谱法测定鲍鱼组织中磷酸甘氨酸的优化方法。
Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00428-5
M. Viant, E. Rosenblum, R. Tjeerdema
{"title":"Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography.","authors":"M. Viant, E. Rosenblum, R. Tjeerdema","doi":"10.1016/S0378-4347(01)00428-5","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00428-5","url":null,"abstract":"","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"146 1","pages":"107-11"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79161434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 42
Novel and simple two-step purification of a full-length rat glucocorticoid-receptor expressed in a baculovirus system. 杆状病毒系统中表达的全长大鼠糖皮质激素受体的新颖和简单的两步纯化。
Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00408-X
M. Hyodo, K. Okamoto, K. Shibata, N. Suematsu, F. Isohashi
{"title":"Novel and simple two-step purification of a full-length rat glucocorticoid-receptor expressed in a baculovirus system.","authors":"M. Hyodo, K. Okamoto, K. Shibata, N. Suematsu, F. Isohashi","doi":"10.1016/S0378-4347(01)00408-X","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00408-X","url":null,"abstract":"","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"6 1","pages":"89-97"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80363772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Direct chiral assay of tramadol and detection of the phase II metabolite O-demethyl-tramadol glucuronide in human urine using capillary electrophoresis with laser-induced native fluorescence detection 曲马多直接手性测定及尿中II期代谢物o -去甲基曲马多葡糖苷的毛细管电泳激光诱导荧光检测
Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 Epub Date: 2001-08-22 DOI: 10.1016/S0378-4347(01)00366-8
Uta Birgitta Soetebeer , Marc-Oliver Schierenberg , Harald Schulz , Peter Andresen , Gottfried Blaschke
{"title":"Direct chiral assay of tramadol and detection of the phase II metabolite O-demethyl-tramadol glucuronide in human urine using capillary electrophoresis with laser-induced native fluorescence detection","authors":"Uta Birgitta Soetebeer ,&nbsp;Marc-Oliver Schierenberg ,&nbsp;Harald Schulz ,&nbsp;Peter Andresen ,&nbsp;Gottfried Blaschke","doi":"10.1016/S0378-4347(01)00366-8","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00366-8","url":null,"abstract":"<div><p>A chiral separation using carboxymethyl-β-cyclodextrin and methyl-β-cyclodextrin for the direct assay of tramadol in human urine by capillary electrophoresis (CE) with laser-induced native fluorescence detection was developed. Furthermore, the phase II metabolite <em>O</em>-demethyl tramadol glucuronide was determined from the urine samples and the ratio of the diasteromers was determined. The chiral method was validated. Correlation coefficients were higher than 0.999. Within day variation showed accuracy in the range 96.1–105.8% with a RSD less than 6.00%. Day to day variation present an accuracy ranging from 100.2 to 103.5% with a RSD less than 5.4%. After oral administration of 150 mg tramadol hydrochloride to a healthy volunteer, the urinary excretion was monitored during 24 h. About 11.4% of the dose was excreted as 1<em>S</em>,2<em>S</em>-tramadol, 16.4% as 1<em>R</em>,2<em>R</em>-tramadol and 23.7% as <em>O</em>-demethyl tramadol glucuronide. The amount of 1<em>S</em>,2<em>S</em> <em>O</em>-demethyl tramadol glucuronide was more than three fold higher as 1<em>R</em>,2<em>R</em>-<em>O</em>-demethyl tramadol glucuronide. The enantiomeric ratio of tramadol and the diastereomeric ratio of <em>O</em>-demethyl tramadol glucuronide was deviated from 1.0 showing that a stereoselective metabolism of tramadol occurs.</p></div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 3-13"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00366-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 30
Determination of hyperforin, hypericin, and pseudohypericin in human plasma using high-performance liquid chromatography analysis with fluorescence and ultraviolet detection 荧光和紫外检测高效液相色谱法测定人血浆中的金丝桃素、金丝桃素和假金丝桃素
Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 Epub Date: 2001-09-30 DOI: 10.1016/S0378-4347(01)00390-5
Steffen Bauer , Elke Störmer , Hans-Joachim Graubaum , Ivar Roots
{"title":"Determination of hyperforin, hypericin, and pseudohypericin in human plasma using high-performance liquid chromatography analysis with fluorescence and ultraviolet detection","authors":"Steffen Bauer ,&nbsp;Elke Störmer ,&nbsp;Hans-Joachim Graubaum ,&nbsp;Ivar Roots","doi":"10.1016/S0378-4347(01)00390-5","DOIUrl":"10.1016/S0378-4347(01)00390-5","url":null,"abstract":"<div><p>Hyperforin, hypericin and pseudohypericin are the main ingredients of St. John’s wort extract, which is available over the counter for treatment of mild to moderate depression. To facilitate clinical studies we developed two sensitive HPLC methods for determination of hypericin/pseudohypericin and hyperforin, respectively, in human plasma samples. The achieved limits of quantitation of 0.25 ng/ml for hypericin and pseudohypericin and 10 ng/ml for hyperforin were low enough to allow determination of pharmacokinetic parameters of the substances. Following liquid–liquid extraction of human plasma the samples were separated by isocratic reversed-phase HLPC and analyzed using fluorimetric detection for hypericin/pseudohypericin and UV detection for hyperforin.</p></div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 29-35"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00390-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89158373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 42
Metabolism of methandrostenolone in the horse: a gas chromatographic–mass spectrometric investigation of phase I and phase II metabolism 马体内甲雄甾酮的代谢:第一期和第二期代谢的气相色谱-质谱研究
Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 Epub Date: 2001-10-02 DOI: 10.1016/S0378-4347(01)00409-1
Andrew R McKinney , Damon D Ridley , Craig J Suann
{"title":"Metabolism of methandrostenolone in the horse: a gas chromatographic–mass spectrometric investigation of phase I and phase II metabolism","authors":"Andrew R McKinney ,&nbsp;Damon D Ridley ,&nbsp;Craig J Suann","doi":"10.1016/S0378-4347(01)00409-1","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00409-1","url":null,"abstract":"<div><p>The phase I and phase II metabolism of the anabolic steroid methandrostenolone was investigated following oral administration to a standardbred gelding. In the phase I study, metabolites were isolated from the urine by solid-phase extraction, deconjugated by acid catalysed methanolysis and converted to their <em>O</em>-methyloxime trimethylsilyl derivatives. GC–MS analysis indicated the major metabolic processes to be sequential reduction of the A-ring and hydroxylation at C6 and C16. In the phase II study, unconjugated, β-glucuronidated and sulfated metabolites were fractionated and deconjugated using a combination of liquid–liquid extraction, enzyme hydrolysis, solid-phase extraction and acid catalysed methanolysis. Derivatization followed by GC–MS analysis revealed extensive conjugation to both glucuronic and sulfuric acids, with only a small proportion of metabolites occurring in unconjugated form.</p></div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 71-79"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00409-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Quantitative analysis of fentanyl in rat plasma by gas chromatography with nitrogen-phosphorus detection. 大鼠血浆中芬太尼的氮磷气相色谱定量分析。
Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00405-4
H. Choi, H. C. Shin, G. Khang, J. M. Rhee, H. B. Lee
{"title":"Quantitative analysis of fentanyl in rat plasma by gas chromatography with nitrogen-phosphorus detection.","authors":"H. Choi, H. C. Shin, G. Khang, J. M. Rhee, H. B. Lee","doi":"10.1016/S0378-4347(01)00405-4","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00405-4","url":null,"abstract":"","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"9 9 1","pages":"63-9"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85466718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 28
Determination of LSD and its metabolites in human biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry. 高效液相色谱-电喷雾串联质谱法测定人体液中LSD及其代谢物。
Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00386-3
J. Canezin, A. Cailleux, A. Turcant, A. Bouil, P. Harry, Pierre Allain
{"title":"Determination of LSD and its metabolites in human biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry.","authors":"J. Canezin, A. Cailleux, A. Turcant, A. Bouil, P. Harry, Pierre Allain","doi":"10.1016/S0378-4347(01)00386-3","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00386-3","url":null,"abstract":"","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"17 1","pages":"15-27"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72776998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 52
Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography 高效液相色谱法测定鲍鱼组织中磷酸甘氨酸的优化方法
Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 Epub Date: 2001-10-05 DOI: 10.1016/S0378-4347(01)00428-5
Mark R Viant, Eric S Rosenblum, Ronald S Tjeerdema
{"title":"Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography","authors":"Mark R Viant,&nbsp;Eric S Rosenblum,&nbsp;Ronald S Tjeerdema","doi":"10.1016/S0378-4347(01)00428-5","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00428-5","url":null,"abstract":"<div><p>A rapid high-performance liquid chromatography method for the determination of phosphoarginine (PArg) in invertebrate tissue has been redeveloped and validated. The method employs a reversed-phase amino column and a KH<sub>2</sub>PO<sub>4</sub>–acetonitrile mobile phase. PArg peak identity was confirmed by comparison with a known standard and via enzymatic conversion. Additionally linear calibration data, low intra-assay variability (&lt;4%), and a detection limit of 5 pmol were determined. The method was demonstrated using PArg extracted from red abalone (<em>Haliotis rufescens</em>) adductor muscle. Validation of the extraction procedure was also completed, including the measurement of a 100.2±0.9% extraction efficiency.</p></div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 107-111"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00428-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 41
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