Journal of Chromatography B: Biomedical Sciences and Applications最新文献_第10页

Author Index Vol. 762 作者索引第762卷

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-10-25 DOI: 10.1016/S0378-4347(01)00398-X

引用次数: 0

High-performance liquid chromatographic assay with ultraviolet detection for quantification of dihydrofluorouracil in human lymphocytes: application to measurement of dihydropyrimidine dehydrogenase activity 紫外检测高效液相色谱法定量测定人淋巴细胞中二氢氟尿嘧啶:应用于二氢嘧啶脱氢酶活性测定

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-10-25 DOI: 10.1016/S0378-4347(01)00359-0

R. Déporte-Féty , M. Picot , M. Amiand , A. Moreau , L. Campion , D. Lanoë , N. Renée , G. Milano

引用次数: 23

Validation of an analytical method for a potent antitumor agent, TZT-1027, in plasma using liquid chromatography–mass spectrometry 高效抗肿瘤药物TZT-1027在血浆中液相色谱-质谱分析方法的验证

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-10-25 DOI: 10.1016/S0378-4347(01)00360-7

Hiroyuki Ochiai , Hiromi Mori , Hideyuki Murata , Toshimi Seki , Keiko Araki , Yoshihiro Kawabe , Koichi Miyazaki , Kunio Tsukamoto , Satoshi Iwamura

{"title":"Validation of an analytical method for a potent antitumor agent, TZT-1027, in plasma using liquid chromatography–mass spectrometry","authors":"Hiroyuki Ochiai , Hiromi Mori , Hideyuki Murata , Toshimi Seki , Keiko Araki , Yoshihiro Kawabe , Koichi Miyazaki , Kunio Tsukamoto , Satoshi Iwamura","doi":"10.1016/S0378-4347(01)00360-7","DOIUrl":"10.1016/S0378-4347(01)00360-7","url":null,"abstract":"<div>A sensitive and specific analytical method for a potent antitumor agent, TZT-1027, in plasma has been developed using liquid chromatography–mass spectrometry (LC–MS) with [2H4]TZT-1027 as an internal standard (I.S.). A plasma sample was purified by solid-phase extraction on a C18 cartridge, followed by solvent extraction with diethyl ether. The extract was then injected into the LC–MS system. Chromatography was carried out on a C18 reversed-phase column using acetonitrile–0.05% trifluoroacetic acid (TFA) (55:45) as a mobile phase. Mass spectrometric analysis was performed in atmospheric pressure chemical ionization (APCI) mode with positive ion detection, and the protonated molecular ions ([M+H]+) of TZT-1027 and I.S. were monitored to allow quantitation. The method was applied to the determination of TZT-1027 in human, monkey, dog, rat and mouse plasma. As far as the sample preparation was concerned, good recoveries (73.5–99.1%) were obtained. The calibration curves were linear over the range of 0.25–100 ng per 1 ml of human, dog and rat plasma, per 0.5 ml of monkey plasma, and per 0.1 ml of mouse plasma. From the intra- and inter-day accuracy and precision, the present method satisfies the accepted criteria for bioanalytical method validation. TZT-1027 was stable when stored below −15°C for 6 months in human plasma and for 3 weeks in plasma from other species. TZT-1027 was also stable in plasma through at least three freeze–thaw cycles.</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"762 2","pages":"Pages 155-163"},"PeriodicalIF":0.0,"publicationDate":"2001-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00360-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81208341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Development and validation of a high-performance liquid chromatography–tandem mass spectrometry assay for the determination of sanfetrinem in human plasma 高效液相色谱-串联质谱法测定人血浆中盐酸异替宁的建立和验证

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-10-25 DOI: 10.1016/S0378-4347(01)00362-0

Claudio De Nardi, Simone Braggio, Luca Ferrari, Stefano Fontana

{"title":"Development and validation of a high-performance liquid chromatography–tandem mass spectrometry assay for the determination of sanfetrinem in human plasma","authors":"Claudio De Nardi, Simone Braggio, Luca Ferrari, Stefano Fontana","doi":"10.1016/S0378-4347(01)00362-0","DOIUrl":"10.1016/S0378-4347(01)00362-0","url":null,"abstract":"<div>A rapid, selective and accurate high-performance liquid chromatography–tandem mass spectrometry assay for the quantification of sanfetrinem in human plasma has been developed and validated. The performance of manual and automated sample preparation was assessed; 50 μl of plasma sample was deproteinized with acetonitrile, followed by dilution with water and injection onto the LC system. Chromatographic separation was achieved on a Phenomenex Luna C18(2), 50×2.0 (5 μm) column with a mobile phase consisting of water–acetonitrile with 0.1% formic acid followed by detection with a Perkin-Elmer API3000 mass spectrometer in multiple reaction monitoring mode. The lower limit of quantification was improved by five times compared to the UV method previously reported. A range of concentration from 10 ng/ml to 5 μg/ml was covered. The method was applied to the quantification of sanfetrinem in human plasma samples from healthy volunteers participating in a clinical study.</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"762 2","pages":"Pages 193-201"},"PeriodicalIF":0.0,"publicationDate":"2001-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00362-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87361641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Electroosmotic properties of microfluidic channels composed of poly(dimethylsiloxane) 聚二甲基硅氧烷微流控通道的电渗透特性

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-10-25 DOI: 10.1016/S0378-4347(01)00327-9

Xueqin Ren , Mark Bachman , Christopher Sims , G.P Li , Nancy Allbritton

{"title":"Electroosmotic properties of microfluidic channels composed of poly(dimethylsiloxane)","authors":"Xueqin Ren , Mark Bachman , Christopher Sims , G.P Li , Nancy Allbritton","doi":"10.1016/S0378-4347(01)00327-9","DOIUrl":"10.1016/S0378-4347(01)00327-9","url":null,"abstract":"<div>Microfluidic devices fabricated from polymers exhibit great potential in biological analyses. Poly(dimethylsiloxane) (PDMS) has shown promise as a substrate for rapid prototyping of devices. Despite this, disagreement exists in the literature as to the ability of PDMS to support electroosmotic (EO) flow and the stability of that flow over time. We demonstrate that in low ionic strength solutions near neutral in pH, oxidized PDMS had a four-fold greater EO mobility (μeo) compared to native PDMS. The greater μeo was maintained irrespective of whether glass or PDMS was used as a support forming one side of the channel. This enhanced μeo was preserved as long as the channels were filled with an aqueous solution. Upon exposure of the channels to air, the mobility decreased by a factor of two with a half-life of 9 h. The EO properties of the air-exposed, oxidized PDMS were regenerated by exposure to strong base. High ionic strength, neutral in pH buffers compatible with living eukaryotic cells diminished the EO flow in the oxidized PDMS devices to a much greater extent than in the native PDMS devices. For analyses utilizing intact and living cells, oxidation of PDMS may not be an effective strategy to substantially increase the μeo.</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"762 2","pages":"Pages 117-125"},"PeriodicalIF":0.0,"publicationDate":"2001-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00327-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80222417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 173

Capillary electrophoresis analysis of nitrite and nitrate in sub-microliter quantities of airway surface liquid 亚微升气道表面液体中亚硝酸盐和硝酸盐的毛细管电泳分析

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-10-25 DOI: 10.1016/S0378-4347(01)00358-9

K Govindaraju, M Toporsian, M.E Ward, D.K Lloyd , E.A Cowley, D.H Eidelman

{"title":"Capillary electrophoresis analysis of nitrite and nitrate in sub-microliter quantities of airway surface liquid","authors":"K Govindaraju, M Toporsian, M.E Ward, D.K Lloyd , E.A Cowley, D.H Eidelman","doi":"10.1016/S0378-4347(01)00358-9","DOIUrl":"10.1016/S0378-4347(01)00358-9","url":null,"abstract":"<div>We developed a simple capillary electrophoresis (CE) method to measure nitrite and nitrate concentrations in sub-microliter samples of rat airway surface liquid (ASL), a thin (10–30 μm) layer of liquid covering the epithelial cells lining the airways of the lung. The composition of ASL has been poorly defined, in large part because of the small sample volume (∼1–3 μl per cm2 of epithelium) and difficulty of harvesting ASL. We have used capillary tubes for ASL sample collection, with microanalysis by CE using a 50 mM phosphate buffer (pH 3), with 0.5 mM spermine as a dynamic flow modifier, and direct UV detection at 214 nm. The limit of detections (LODs), under conditions used, for ASL analysis were 10 μM for nitrate and 30 μM for nitrite (S/N=3). Nitrate and nitrite were also measured in rat plasma. The concentration of nitrate was 102±12 μM in rat ASL and 70±1.0 μM in rat plasma, whereas nitrite was 83±28 μM in rat ASL and below the LOD in rat plasma. After instilling lipopolysaccharide intratracheally to induce increased NO production, the nitrate concentration in ASL increased to 387±16 μM, and to 377±88 μM in plasma. The concentration of nitrite increased to 103±7.0 μM for ASL and 138±17 μM for plasma.</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"762 2","pages":"Pages 147-154"},"PeriodicalIF":0.0,"publicationDate":"2001-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00358-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80445227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Compound Index Vol. 762 复合指数第762卷

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-10-25 DOI: 10.1016/S0378-4347(01)00399-1

引用次数: 0

Simultaneous high-performance liquid chromatography determination of carbamazepine and five of its metabolites in plasma of epileptic patients 同时高效液相色谱法测定癫痫患者血浆中卡马西平及其5种代谢物

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-10-25 DOI: 10.1016/S0378-4347(01)00328-0

Roberto Mandrioli , Fiorenzo Albani , Giovanna Casamenti , Cesare Sabbioni , Maria Augusta Raggi

{"title":"Simultaneous high-performance liquid chromatography determination of carbamazepine and five of its metabolites in plasma of epileptic patients","authors":"Roberto Mandrioli , Fiorenzo Albani , Giovanna Casamenti , Cesare Sabbioni , Maria Augusta Raggi","doi":"10.1016/S0378-4347(01)00328-0","DOIUrl":"10.1016/S0378-4347(01)00328-0","url":null,"abstract":"<div>A high-performance liquid chromatographic method with UV detection for the simultaneous analysis of the antiepileptic drug carbamazepine and five of its metabolites in human plasma has been developed. The analysis was carried out on a reversed-phase column (C8, 150×4.6 mm I.D., 5 μm) using acetonitrile, methanol and a pH 1.9 phosphate buffer as the mobile phase. Under these chromatographic conditions, carbamazepine and its metabolites 10,11-dihydro-10,11-epoxycarbamazepine, 10,11-dihydro-10,11-dihydroxycarbamazepine, 2-hydroxycarbamazepine, 3-hydroxycarbamazepine and 10,11-dihydro-10-hydroxycarbamazepine are baseline separated in less than 18 min. The extraction of the analytes from plasma samples was performed by means of an original solid-phase extraction procedure using Oasis HLB cartridges. The method requires only 250 μl of plasma for one complete analysis. The repeatability (RSD%<2.4), intermediate precision (RSD%<3.5) and extraction yield (84.8–103.0%) were very good for all analytes. The method is suitable for reliable therapeutic drug monitoring of patients undergoing chronic treatment with carbamazepine and for kinetic–metabolic studies of this drug.</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"762 2","pages":"Pages 109-116"},"PeriodicalIF":0.0,"publicationDate":"2001-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00328-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90109672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 63

High-performance liquid chromatographic assay for abacavir and its two major metabolites in human urine and cerebrospinal fluid 高效液相色谱法测定人尿和脑脊液中阿巴卡韦及其两种主要代谢物

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-10-25 DOI: 10.1016/S0378-4347(01)00361-9

Joshua R Ravitch, Caroline G Moseley

引用次数: 21

Gas chromatographic–mass spectrometric method for quantitation of phenylalanine and tyrosine in serum 气相色谱-质谱法测定血清中苯丙氨酸和酪氨酸

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-10-25 DOI: 10.1016/S0378-4347(01)00339-5

J.B Laurens, X.Y Mbianda, J.B Ubbink, W.J.H Vermaak

引用次数: 8