Journal of Chromatography B: Biomedical Sciences and Applications最新文献_第2页

Retention of bile salts in micellar electrokinetic chromatography: relation of capacity factor to octanol–water partition coefficient and critical micellar concentration 胶束电动色谱中胆盐的保留:容量因子与辛醇-水分配系数和临界胶束浓度的关系

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-25 Epub Date: 2001-10-05 DOI: 10.1016/S0378-4347(01)00417-0

Silvia E Lucangioli , Clyde N Carducci , Valeria P Tripodi , Ernst Kenndler

{"title":"Retention of bile salts in micellar electrokinetic chromatography: relation of capacity factor to octanol–water partition coefficient and critical micellar concentration","authors":"Silvia E Lucangioli , Clyde N Carducci , Valeria P Tripodi , Ernst Kenndler","doi":"10.1016/S0378-4347(01)00417-0","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00417-0","url":null,"abstract":"<div><p>The capacity factors of 16 anionic cholates (from six bile salts, including their glyco- and tauro-conjugates) were determined in a micellar electrokinetic chromatography (MEKC) system consisting of buffer, pH 7.5 (phosphate–boric acid; 20 mmol/l) with 50 mmol/l sodium dodecyl sulfate (SDS) as micelle former and 10% acetonitrile as organic modifier. The capacity factors of the fully dissociated, negatively charged analytes (ranging between 0.2 and 60) were calculated from their mobilities, with a reference background electrolyte (BGE) without SDS representing “free” solution. For comparison, the capacity factors were derived for a second reference BGE where the SDS concentration (5 mmol/l) is close to the critical micellar concentration (CMC). The capacity factors are compared with the logarithm of the octanol–water partition coefficient, log <em>P</em><sub>OW</sub>, as measure for lipophilicity. Clear disagreement between these two parameters is found especially for epimeric cholates with the hydroxy group in position 7. In contrast, fair relation between the capacity factor of the analytes and their CMC is observed both depending strongly on the orientation of the OH groups, and tauro-conjugation as well. In this respect the retention behaviour of the bile salts in MEKC seems to reflect their role as detergents in living systems, and might serve as model parameter beyond lipophilicity.</p></div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 2","pages":"Pages 113-120"},"PeriodicalIF":0.0,"publicationDate":"2001-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00417-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91741844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Thin-layer chromatography blotting for the fluorescence detection of phospholipid hydroperoxides and cholesteryl ester hydroperoxides 薄层色谱法荧光检测磷脂氢过氧化物和胆固醇酯氢过氧化物

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-25 Epub Date: 2001-10-09 DOI: 10.1016/S0378-4347(01)00426-1

Junji Terao, Mariko Miyoshi, Sayuri Miyamoto

引用次数: 16

Quantitative detection of bisphenol A and bisphenol A diglycidyl ether metabolites in human plasma by liquid chromatography–electrospray mass spectrometry 液相色谱-电喷雾质谱法定量检测人血浆中双酚A和双酚A二缩水甘油醚代谢物

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-25 Epub Date: 2001-10-02 DOI: 10.1016/S0378-4347(01)00393-0

Koichi Inoue , Akiko Yamaguchi , Megumi Wada , Yoshihiro Yoshimura , Tsunehisa Makino , Hiroyuki Nakazawa

{"title":"Quantitative detection of bisphenol A and bisphenol A diglycidyl ether metabolites in human plasma by liquid chromatography–electrospray mass spectrometry","authors":"Koichi Inoue , Akiko Yamaguchi , Megumi Wada , Yoshihiro Yoshimura , Tsunehisa Makino , Hiroyuki Nakazawa","doi":"10.1016/S0378-4347(01)00393-0","DOIUrl":"10.1016/S0378-4347(01)00393-0","url":null,"abstract":"<div><p>Due to the ubiquity of epoxy resin compounds and their potential role in increasing the risk for reproductive dysfunction and cancer, the need for an assessment of human exposure is urgent. Therefore, we developed a method for measuring bisphenol A (BPA) and bisphenol A diglycidyl ether (BADGE) metabolites in human blood samples using high-performance liquid chromatography–electrospray ionization mass spectrometry (LC–MS). Human blood samples were processed using enzymatic deconjugation of the glucuronides followed by a novel sample preparation procedure using a solid-phase-cartridge column. This selective analytical method permits rapid detection of the metabolites, free BPA and a hydrolysis product of BADGE (BADGE-4OH) with detection limits in the low nanogram per milliliter range (0.1 ng ml<sup>−1</sup> of BPA and 0.5 ng ml<sup>−1</sup> of BADGE-4OH). The sample extraction was achieved by Oasis HLB column on gradient elution. The recoveries of BPA and BADGE-4OH added to human plasma samples were above 70.0% with a standard deviation of less than 5.0%. This selective, sensitive and accurate method will assist in elucidating potential associations between human exposure to epoxy-based compounds and adverse health effects.</p></div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 2","pages":"Pages 121-126"},"PeriodicalIF":0.0,"publicationDate":"2001-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00393-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81275719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 63

Erratum to “Simultaneous determination of the histamine H1-receptor antagonist ebastine and its two metabolites, carebastine and hydroxyebastine, in human plasma using high-performance liquid chromatography” 《高效液相色谱法同时测定人血浆中组胺h1受体拮抗剂依巴斯汀及其两种代谢物卡伐巴斯汀和羟伐巴斯汀》的勘误

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-25 Epub Date: 2001-11-19 DOI: 10.1016/S0378-4347(01)00534-5

Michiaki Matsuda, Yasuyuki Mizuki, Yoshiaki Terauchi

引用次数: 3

Determination of transdermal sildenafil in nude mouse skin by reversed-phase high-performance liquid chromatography 反相高效液相色谱法测定裸鼠皮肤透皮中西地那非的含量

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-25 Epub Date: 2001-11-19 DOI: 10.1016/S0378-4347(01)00421-2

Jiahorng Liaw , Ting-Wei Chang

引用次数: 29

Influence of various biological matrices (plasma, blood microdialysate) on chromatographic performance in the determination of β-blockers using an alkyl-diol silica precolumn for sample clean-up 不同生物基质(血浆、血液微透析液)对样品净化用烷基二醇硅前柱测定β-阻滞剂色谱性能的影响

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-25 Epub Date: 2001-10-17 DOI: 10.1016/S0378-4347(01)00423-6

Csilla Mišl’anová , Milan Hutta

引用次数: 23

Quantitative analysis of fentanyl in rat plasma by gas chromatography with nitrogen–phosphorus detection 大鼠血浆中芬太尼的氮磷气相色谱定量分析

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 Epub Date: 2001-10-02 DOI: 10.1016/S0378-4347(01)00405-4

Hak Soo Choi , Ho-Chul Shin , Gilson Khang , John M Rhee , Hai Bang Lee

引用次数: 28

High performance liquid chromatographic analysis of St. John’s Wort with photodiode array detection 光电二极管阵列检测圣约翰草的高效液相色谱分析

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 Epub Date: 2001-10-02 DOI: 10.1016/S0378-4347(01)00404-2

Wenkui Li, John F Fitzloff

{"title":"High performance liquid chromatographic analysis of St. John’s Wort with photodiode array detection","authors":"Wenkui Li, John F Fitzloff","doi":"10.1016/S0378-4347(01)00404-2","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00404-2","url":null,"abstract":"<div><p>An RP-HPLC method with photodiode array detection was established for the determination of major constituents (rutin, hyperoside, isoquercitrin, quercitrin, quercetin, pseudohypericin, hyperforin and hypericin) in St. John’s Wort dietary supplements. The samples were extracted with methanol by means of sonication in low temperature. The extraction was rapid, with two steps of sonication (30 min each) recovering more than 99% of the major constituents in St. John’s Wort samples. The major components were separated by RP-18 chromatography column using a 60-min water–acetonitrile–methanol–trifluoroacetic acid gradient. The quantification was performed by using external standards. Sample preparation and stability of methanolic extract of St. John’s Wort were extensively explored. It is worth noting that the major constituents in the methanolic extract of St John’s Wort, especially hypericin and pseudohypericin, might be retained by some filter cartridges during the filtration. The current method may serve as a valuable tool for the QA/QC of St. John’s Wort dietary supplements.</p></div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 99-105"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00404-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91700988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 59

Determination of LSD and its metabolites in human biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry 高效液相色谱-电喷雾串联质谱法测定人体液中LSD及其代谢物

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 Epub Date: 2001-09-05 DOI: 10.1016/S0378-4347(01)00386-3

J Canezin , A Cailleux , A Turcant , A Le Bouil , P Harry , P Allain

{"title":"Determination of LSD and its metabolites in human biological fluids by high-performance liquid chromatography with electrospray tandem mass spectrometry","authors":"J Canezin , A Cailleux , A Turcant , A Le Bouil , P Harry , P Allain","doi":"10.1016/S0378-4347(01)00386-3","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00386-3","url":null,"abstract":"<div><p>A liquid chromatographic procedure with electrospray ionization tandem mass spectrometric detection has been developed and validated for LSD and iso-LSD determination. A one-step liquid–liquid extraction on 1 ml blood or urine was used. The lower limit for quantitative determination was 0.02 μg/l for LSD and iso-LSD. The analytical procedure has been applied in two positive cases (case 1: LSD=0.31 μg/l, iso-LSD=0.27 μg/l in plasma and LSD=1.30 μg/l, iso-LSD=0.82 μg/l in urine; case 2: LSD=0.24 μg/l, iso-LSD=0.6 μg/l in urine). LSD metabolism was investigated using MS–MS neutral loss monitoring for the screening of potential metabolites. The main metabolite was 2-oxo-3-hydroxy-LSD (O–H–LSD) present in urine at the concentrations of 2.5 μg/l and 6.6 μg/l, respectively, for case 1 and 2, and was not present in plasma. Nor-LSD was also found in urine at 0.15 and 0.01 μg/l levels. Nor-iso-LSD, lysergic acid ethylamide (LAE), trioxylated-LSD, lysergic acid ethyl-2-hydroxyethylamide (LEO) and 13 and 14-hydroxy-LSD and their glucuronide conjugates were detected in urine using specific MS–MS transitions.</p></div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 15-27"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00386-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 53

Determination of BMS-284756, a new quinolone, in mouse serum by high-performance liquid chromatography with fluorescence detection. 高效液相色谱-荧光法测定小鼠血清中新型喹诺酮类药物BMS-284756的含量。

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00394-2

D. Xuan, C. Turley, C. Nightingale, D. Nicolau

引用次数: 4