Influence of various biological matrices (plasma, blood microdialysate) on chromatographic performance in the determination of β-blockers using an alkyl-diol silica precolumn for sample clean-up

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-25 DOI:10.1016/S0378-4347(01)00423-6

Csilla Mišl’anová , Milan Hutta

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引用次数: 23

Abstract

A HPLC column-switching system with LiChrospher RP-8 ADS precolumn was applied for the determination of beta-blockers (atenolol, pindolol, propranolol) in human plasma. The influence of biological matrices on the changes of the chromatographic parameters such as retention time, peak symmetry, area and selectivity were investigated. After injection of 5 ml plasma a decrease of retention times of the analytes was observed of up to 25% and an increase of asymmetry factors of up to 5%. Peak areas and selectivities were not changed. The observed effect could indicate changes of chromatographic performance caused by contributions of the analytical column or the ADS precolumn. The experiments with microdialysis excluded the contribution of the analytical column. A detailed investigation of experiments have been discussed in this paper.

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不同生物基质(血浆、血液微透析液)对样品净化用烷基二醇硅前柱测定β-阻滞剂色谱性能的影响

采用LiChrospher RP-8 ADS前柱高效液相色谱柱切换系统测定人血浆中β受体阻滞剂(阿替洛尔、品多洛尔、普萘洛尔)的含量。考察了生物基质对保留时间、峰对称、面积和选择性等色谱参数变化的影响。注射5ml血浆后，观察到分析物的保留时间减少高达25%，不对称因子增加高达5%。峰面积和选择性没有改变。所观察到的影响可能表明分析柱或ADS预柱的贡献引起了色谱性能的变化。微透析实验排除了分析柱的贡献。本文进行了详细的实验研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Chromatography B: Biomedical Sciences and Applications

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