Journal of Chromatography B: Biomedical Sciences and Applications最新文献_第4页

Optimized method for the determination of phosphoarginine in abalone tissue by high-performance liquid chromatography 高效液相色谱法测定鲍鱼组织中磷酸甘氨酸的优化方法

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00428-5

Mark R Viant, Eric S Rosenblum, Ronald S Tjeerdema

引用次数: 41

Stereoselective analysis of fluvastatin in human plasma for pharmacokinetic studies 立体选择分析氟伐他汀在人血浆中的药代动力学研究

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00407-8

Vera Lucia Lanchote , Adriana Rocha , Flávio Ulliana Vieira de Albuquerque , Eduardo Barbosa Coelho , Pierina Sueli Bonato

{"title":"Stereoselective analysis of fluvastatin in human plasma for pharmacokinetic studies","authors":"Vera Lucia Lanchote , Adriana Rocha , Flávio Ulliana Vieira de Albuquerque , Eduardo Barbosa Coelho , Pierina Sueli Bonato","doi":"10.1016/S0378-4347(01)00407-8","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00407-8","url":null,"abstract":"<div>Fluvastatin, an inhibitor of cholesterol biosynthesis, is commercialized as a racemic mixture of the (+)-3R,5S and (−)-3S,5R stereoisomers, although inhibition of HMG-CoA reductase mainly resides in the (+)-(3R,5S)-fluvastatin isomer. The aim of the present study was to analyze fluvastatin isomers in human plasma with application to studies on kinetic disposition. Plasma samples of 1 ml were eluted into 3 ml LC-18 Supelclean (Supelco) columns equilibrated with methanol and water. The columns were washed with water and acetonitrile and then eluted with methanol containing 0.2% diethylamine. The (+)-3R,5S and (−)-3S,5R isomers were separated by HPLC on a Chiralcel OD-H chiral phase column and detected by fluorescence (λex 305 nm; λem 390 nm). The quantification limit was 0.75 ng for each isomer/ml plasma and linearity was observed up to 625 ng/ml. The relative standard deviations obtained for intra- and inter-assay precision were lower than 10% and the recovery was higher than 80% for both enantiomers. Application of the method to a stereoselective study on the pharmacokinetics of fluvastatin administered as a single oral dose (Lescol, 20 mg) to a healthy volunteer revealed stereoselectivity, with the highest plasma concentrations being observed for the (−)-3S,5R isomer (Cmax 92.4 vs. 60.3 ng/ml, AUC0–∞ 133.3 vs. 97.4 ng h/ml, Cl/f 150.2 vs. 205.2 l h−1 and Vd/f 4.4 vs. 6.0 l/kg).</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 81-88"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00407-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Determination of BMS-284756, a new quinolone, in mouse serum by high-performance liquid chromatography with fluorescence detection 高效液相色谱-荧光法测定小鼠血清中新型喹诺酮类药物BMS-284756的含量

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00394-2

Dawei Xuan , Christina Turley , Charles H Nightingale , David P Nicolau

{"title":"Determination of BMS-284756, a new quinolone, in mouse serum by high-performance liquid chromatography with fluorescence detection","authors":"Dawei Xuan , Christina Turley , Charles H Nightingale , David P Nicolau","doi":"10.1016/S0378-4347(01)00394-2","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00394-2","url":null,"abstract":"<div>A sensitive, simple, and accurate method for determination of BMS-284756, a novel des-F(6)-quinolone antimicrobial agent in mouse serum was developed by HPLC with fluorescence detection. Sample preparations were carried out by protein precipitation with the addition of acetonitrile, followed by evaporation of the acetonitrile to dryness. The resultant residual was then reconstituted in 0.01 M HCl and injected onto a Nucleosil 100 10 μm, C18 25 cm×4.6 mm analytical column. The mobile phase consisted of acetonitrile–0.01 M NaH2PO4 (20:80, v/v) with 0.01 M tetrabutylammonium hydrogen sulfate. The fluorescence of the column effluent was monitored at an excitation wavelength of 290 nm and an emission wavelength of 418 nm. The assay was shown to be linear from 0.2 to 10.0 μg/ml (R2=0.998). Mean recovery was determined as 95.1%. Inter- and intra-assay precisions were <6% RSD. The HPLC method developed has been applied to determine the pharmacokinetics of BMS-284756 in a murine bacterial infection model.</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 37-43"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00394-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Metabolism of methandrostenolone in the horse: a gas chromatographic-mass spectrometric investigation of phase I and phase II metabolism. 马体内甲雄甾酮的代谢:第一期和第二期代谢的气相色谱-质谱研究。

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00409-1

A. R. McKinney, D. Ridley, C. Suann

引用次数: 15

Chromatographic resolution of the enantiomers of phenylpropanolamine by using molecularly imprinted polymer as the stationary phase. 用分子印迹聚合物作为固定相进行苯丙醇胺对映体的色谱分离。

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00397-8

C. Hwang, W. Lee

引用次数: 39

Chromatographic resolution of the enantiomers of phenylpropanolamine by using molecularly imprinted polymer as the stationary phase 用分子印迹聚合物作为固定相进行苯丙醇胺对映体的色谱分离

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00397-8

Ching-Chiang Hwang, Wen-Chien Lee

{"title":"Chromatographic resolution of the enantiomers of phenylpropanolamine by using molecularly imprinted polymer as the stationary phase","authors":"Ching-Chiang Hwang, Wen-Chien Lee","doi":"10.1016/S0378-4347(01)00397-8","DOIUrl":"https://doi.org/10.1016/S0378-4347(01)00397-8","url":null,"abstract":"<div>In this study molecular imprinting technology was employed to prepare a specific affinity sorbent for the resolution of phenylpropanolamine, a chiral drug. The molecularly imprinted polymer (MIP) was prepared by non-covalent molecular imprinting with either (−)- or (+)-phenylpropanolamine as the template. Methacrylic acid and ethylene glycol dimethacrylate were copolymerized in the presence of the template molecule. The bulk polymerization was carried out in chloroform with 2,2′-azobisisobutyronitrile as the initiator, at 4°C and under UV radiation. The resulting MIP was ground into powders, which were slurry packed into analytical columns. After removal of template molecules, the MIP-packed columns were found to be effective for the resolution of (±)-phenylpropanolamine racemates. The separation factor for the enantiomers ranged between 1.8 and 3.8 when the column was packed with MIP prepared with (+)-phenylpropanolamine as the template. A separation factor ranging from 2.1 to 3.6 could be achieved from the column packed with MIP, prepared with (−)-phenylpropanolamine as the template. Although the separation factor was higher with that previously obtained from reversed-phase column chromatography following derivatization with a chiral agent, elution peaks were broader due to the heterogeneity of binding sites on MIP particles and the possible non-specific interaction.</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"765 1","pages":"Pages 45-53"},"PeriodicalIF":0.0,"publicationDate":"2001-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00397-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91701595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40

Novel and simple two-step purification of a full-length rat glucocorticoid-receptor expressed in a baculovirus system 杆状病毒系统中表达的全长大鼠糖皮质激素受体的新颖和简单的两步纯化

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-12-05 DOI: 10.1016/S0378-4347(01)00408-X

Makoto Hyodo , Kazuki Okamoto , Kiyotaka Shibata , Naoya Suematsu , Fumihide Isohashi

引用次数: 3

Separation methods applicable to prostate cancer diagnosis and monitoring therapy 分离方法适用于前列腺癌的诊断和监测治疗。

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-11-25 DOI: 10.1016/S0378-4347(01)00245-6

Shuhei Sumi, Kyoko Arai, Ken-ichiro Yoshida

{"title":"Separation methods applicable to prostate cancer diagnosis and monitoring therapy","authors":"Shuhei Sumi, Kyoko Arai, Ken-ichiro Yoshida","doi":"10.1016/S0378-4347(01)00245-6","DOIUrl":"10.1016/S0378-4347(01)00245-6","url":null,"abstract":"<div>During the last decade, significant research has been conducted using prostate-specific antigen (PSA) in the basic and clinical sciences and many advances have occurred in the clinical use of PSA for detecting and monitoring prostate cancer (PCa). Separation methods including gel-permeation chromatography, isoelectric focusing, lectin-affinity chromatography, polyacrylamide gel electrophoresis and high-performance liquid chromatography have made significant contributions to the discovery and identification of different molecular forms of PSA. Furthermore, the measurement of free and total PSA has improved the ability of PSA to detect early PCa. However, unnecessary biopsies are still needed for men with slightly elevated PSA values. On the other hand, PSA is not adequate for staging newly diagnosed PCa and prognosticating the course in individual cases. The possible application of separation methods in the basic science of prostate cancer may be associated with identification of more cancer-specific forms of PSA and discoveries of other serum proteins useful not only for detecting, but also for staging and prognosticating PCa. Such novel markers might lead to a better understanding of PCa aggressiveness and to developments in the clinical field of treatment.</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"764 1","pages":"Pages 445-455"},"PeriodicalIF":0.0,"publicationDate":"2001-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00245-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78348077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Separation of finasteride and analogues 非那雄胺及其类似物的分离。

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-11-25 DOI: 10.1016/S0378-4347(01)00317-6

J. Macek

引用次数: 11

Separation methods for anthraquinone related anti-cancer drugs 蒽醌类相关抗癌药物的分离方法。

Journal of Chromatography B: Biomedical Sciences and Applications Pub Date : 2001-11-25 DOI: 10.1016/S0378-4347(01)00281-X

Paul M Loadman , Christopher R Calabrese

{"title":"Separation methods for anthraquinone related anti-cancer drugs","authors":"Paul M Loadman , Christopher R Calabrese","doi":"10.1016/S0378-4347(01)00281-X","DOIUrl":"10.1016/S0378-4347(01)00281-X","url":null,"abstract":"<div>The quinoid anthracycline-related anti-cancer agents represent an important group of anti-tumour drugs with a wide spectrum of activity. We review here some of the separation techniques used for the analysis of anthracyclines and related compounds. In this review we have covered a range of compounds from the early anthracycline antibiotics such as doxorubicin to the more recent anthracenediones and anthrapyrazoles such as mitoxantrone and losoxantrone, respectively. We also include novel compounds such as AQ4N and C1311, both awaiting clinical trial. Separations of the anthraquinone related anti-cancer agents are predominantly by HPLC. These separation techniques have been used for a variety of applications including drug stability, protein binding and therapeutic drug monitoring as well as detailed pharmacokinetic and metabolic studies. Pharmacokinetics, and therefore drug analysis, plays a central role in both the development of new agents and also leads to a better understanding of clinically established agents in this class. Sample preparation and extraction methods including solid-phase and liquid–liquid extraction have also been highlighted. Many anthraquinone related compounds are highly coloured and fluoresce. They are suitable for a range of detection methods including UV–Vis, electrochemical and fluorescence. The methods described are used for sometimes complex separations that are needed for the evaluation of such compounds in biological samples.</div>","PeriodicalId":15463,"journal":{"name":"Journal of Chromatography B: Biomedical Sciences and Applications","volume":"764 1","pages":"Pages 193-206"},"PeriodicalIF":0.0,"publicationDate":"2001-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0378-4347(01)00281-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74093688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43