Journal of chromatographic science最新文献_第9页

Ecofriendly HPLC Method for Simultaneous Determination of the Co-Prescribed Drugs in Chemotherapy Omeprazole, Ondansetron and Deflazacort in Spiked Human Plasma. 环境友好高效液相色谱法同时测定人血浆中加标药物奥美拉唑、昂丹司琼和地氮唑。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-11-01 DOI: 10.1093/chromsci/bmad088

Fatma F Abdallah, M Fatma, Ahmed M Gouda, Eglal A Abdelaleem, Aml A Emam

{"title":"Ecofriendly HPLC Method for Simultaneous Determination of the Co-Prescribed Drugs in Chemotherapy Omeprazole, Ondansetron and Deflazacort in Spiked Human Plasma.","authors":"Fatma F Abdallah, M Fatma, Ahmed M Gouda, Eglal A Abdelaleem, Aml A Emam","doi":"10.1093/chromsci/bmad088","DOIUrl":"10.1093/chromsci/bmad088","url":null,"abstract":"Green, selective and accurate high-performance liquid chromatography (HPLC) chromatographic method is presented for simultaneous separation and quantitation of the co-prescribed drugs in chemotherapy omeprazole, ondansetron and deflazacort in spiked human plasma. An isocratic HPLC separation was performed on X Bridge C18 (4.6 × 250 mm) column with 5 μm particle size using mobile phase consisting of methanol: ammonium acetate buffer pH 4 adjusted by acetic acid (60: 40, v/v). The injection volume was 20μL with UV detection wavelength at 237 nm at room temperature. Flow rate of the mobile phase was adjusted to be 2.0 ml/min. Dexamethasone was used as internal standard to correct the variation during sample pretreatment. FDA guidelines were followed to validate the developed method. Successful application of the developed method was revealed by simultaneous determination of omeprazole, ondansetron and deflazacort in spiked human plasma in ranges of 1-20, 0.1-8 and 0.2-8 μg mL-1 for omeprazole, ondansetron and deflazacort, respectively. Four greenness assessment tools were used to evaluate the greenness of the developed method and the results were accepted. This method permitted the accurate simultaneous determination of the studied drugs, thus it can be used during therapeutic drug monitoring in daily clinical practice.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"845-853"},"PeriodicalIF":1.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138299185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reverse Phase HPLC Methodology for the Determination of Bay K8644. 反相高效液相色谱法测定 Bay K8644。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-11-01 DOI: 10.1093/chromsci/bmae011

Pablo Miranda, Analía Castro, Luisina Rodríguez Grassi, Danilo Davyt, Marcelo Hill, Mercedes Segovia, Alvaro W Mombrú, Helena Pardo

{"title":"Reverse Phase HPLC Methodology for the Determination of Bay K8644.","authors":"Pablo Miranda, Analía Castro, Luisina Rodríguez Grassi, Danilo Davyt, Marcelo Hill, Mercedes Segovia, Alvaro W Mombrú, Helena Pardo","doi":"10.1093/chromsci/bmae011","DOIUrl":"10.1093/chromsci/bmae011","url":null,"abstract":"Following ICH guidelines for analytical validation, we report a common C18 column stability indicating isocratic reverse phase high performance liquid chromatography method for the determination of the ion channel modulator Bay K8644. Two main forced degradation products and a minor impurity were also tentatively identified by Mass Spectrometry. The mobile phase consisted of a 50/50 acetonitrile/buffer mixture at a flow rate of 2 mL/min. Mean retention time for Bay K8644 was 3.030 minutes. Excellent linearity (r = 0.9998) was achieved in the range 0.10-1.40 μg/mL at 274 nm wavelength. Analytical limits were 16.56 ± 1.04 ng/mL for detection and 55.21 ± 3.48 ng/mL for quantitation respectively. Accuracy and precision studies showed good results (95-105%). Robustness was assessed by varying ±3%, both temperature and flow rate. Five different stress conditions were applied to assess Bay K8644's stability. Only basic and photolytic treatments yielded degradation products, both correctly resolved in a total runtime of 4 minutes. In conclusion, we developed a fast, simple, sensitive, accurate, precise, reliable and stability indicating method for detecting/quantifying Bay K8644, and tentatively characterized its main impurities/forced degradation products.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"829-836"},"PeriodicalIF":1.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140326636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green TLC-Densitometric Method for Simultaneous Determination of Antazoline and Tetryzoline: Application to Pharmaceutical Formulation and Rabbit Aqueous Humor. 同时测定安他唑啉和泰特唑啉的绿色 TLC 密度测定法：在药物制剂和家兔水体中的应用

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-11-01 DOI: 10.1093/chromsci/bmad042

Ola G Hussein, Yasmin Rostom, Mohamed Abdelkawy, Mamdouh R Rezk, Dina A Ahmed

{"title":"Green TLC-Densitometric Method for Simultaneous Determination of Antazoline and Tetryzoline: Application to Pharmaceutical Formulation and Rabbit Aqueous Humor.","authors":"Ola G Hussein, Yasmin Rostom, Mohamed Abdelkawy, Mamdouh R Rezk, Dina A Ahmed","doi":"10.1093/chromsci/bmad042","DOIUrl":"10.1093/chromsci/bmad042","url":null,"abstract":"Ophthalmic pharmaceutical preparation containing antazoline (ANT) and tetryzoline (TET) is prescribed widely as an over the counter medication for allergic conjunctivitis treatment. Development of a selective, simple and environmentally friendly thin-layer chromatographic method established to determine both ANT and TET in their pure forms, pharmaceutical formulation and spiked aqueous humor samples. By using silica gel plates and means of a developing system consists of ethyl acetate:ethanol (5:5, by volume), the studied drugs separation was achieved, and scanning was carried out at 220.0 nm for the separated bands with a 0.2-18.0 μg/band concentration range for each of ANT and TET. Standard addition technique application was carried out to determine the proposed method validity. Statistical comparison was made between the proposed method and the official methods ANT and TET showing no significant difference concerning accuracy and precision. Furthermore, greenness profile assessment was accomplished by means of four metric tools, namely, analytical greenness, green analytical procedure index, analytical eco-scale and national environmental method index.Highlights.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"807-814"},"PeriodicalIF":1.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9988749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Validation of a SPE-HPLC Method for Quantification of Rhein, Emodin, Chrysophanol and Physcion in Rhamnus petiolaris Boiss. & Balansa. 用于定量检测 Rhamnus petiolaris Boiss.

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-11-01 DOI: 10.1093/chromsci/bmad053

Pelin Köseoğlu Yılmaz, Ufuk Kolak

{"title":"Development and Validation of a SPE-HPLC Method for Quantification of Rhein, Emodin, Chrysophanol and Physcion in Rhamnus petiolaris Boiss. & Balansa.","authors":"Pelin Köseoğlu Yılmaz, Ufuk Kolak","doi":"10.1093/chromsci/bmad053","DOIUrl":"10.1093/chromsci/bmad053","url":null,"abstract":"Anthraquinones exhibit a significant group of natural and synthetic quinone derivatives because of their biological activities and industrial applications. Rhamnaceae is one of the families known to contain different kinds of anthraquinones. In this study, it was aimed to quantify rhein, emodin, chrysophanol and physcion in fruits of Rhamnus petiolaris Boiss. & Balansa belonging to Rhamnaceae by solid phase extraction and high performance liquid chromatography with ultraviolet detection. The anthraquinones were separated using a C18 analytical column. Gradient elution was performed using a mobile phase consisted of 0.1% o-phosphoric acid solution and methanol. Analytes were detected at 254 nm. Calibration curves were prepared in the range of 0.25-5.00 μg/mL for rhein, chrysophanol, physcion, 1.00-50.00 μg/mL for emodin. Limits of detection and quantification were between 0.07-0.11 and 0.20-0.34 μg/mL, respectively. Relative standard deviations were ≤ 5.78% in repeatability and intermediate precision studies. Accuracy was determined as relative mean error (8.17-12.06%). Extraction was achieved by maceration with acetone and ethanol, followed by hydrophilic-lipophilic balance solid phase extraction. Recoveries were between 96.2 and 109.6%. The developed and validated method was successfully performed to quantify rhein, emodin, chrysophanol and physcion in R. petiolaris fruit extracts. Only physcion was not detected above limit of detection.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"872-877"},"PeriodicalIF":1.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530379/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10259909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Validation of a Simple High-Pressure Liquid Chromatography-Ultraviolet Detection Method for Simultaneous Quantitation of First-Line Anti-Tuberculosis Drugs in Formulations of Fixed-Dose Combination. 用于固定剂量复方制剂中一线抗结核药物同步定量分析的简易高压液相色谱-紫外检测法的开发与验证

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-11-01 DOI: 10.1093/chromsci/bmae023

Sudha Vilvamani, Santhanamahalingam Mahalingam, Sruthi Nhavilthodi, Dharman Murugesan, Shanmugam Murugaiha Jeyakumar

{"title":"Development and Validation of a Simple High-Pressure Liquid Chromatography-Ultraviolet Detection Method for Simultaneous Quantitation of First-Line Anti-Tuberculosis Drugs in Formulations of Fixed-Dose Combination.","authors":"Sudha Vilvamani, Santhanamahalingam Mahalingam, Sruthi Nhavilthodi, Dharman Murugesan, Shanmugam Murugaiha Jeyakumar","doi":"10.1093/chromsci/bmae023","DOIUrl":"10.1093/chromsci/bmae023","url":null,"abstract":"The current treatment protocol for drug-sensitive tuberculosis involves all four first-line anti-tuberculosis drugs: rifampicin, isoniazid, pyrazinamide and ethambutol hydrochloride in a single tablet, known as fixed-dose combination tablets. However, the analytical methods are scanty to test all these drugs simultaneously in a single run without any pre-sample process or using a simple method suitable for resource-limited settings. In this method, 50 mM potassium phosphate buffer containing 0.2% triethylamine (without pH adjustment) added with acetonitrile (98:2, v/v) was served as mobile phase A, while mobile phase B was 100% acetonitrile. All four drugs were separated within 10.3 min using a gradient mobile phase program in a C18 column (150 mm × 4.6 mm; 5 μm) and detected at two ultraviolet wavelengths (238 nm for rifampicin, isoniazid and pyrazinamide, and 210 nm for ethambutol hydrochloride). The method was selective, sensitive and linear with a correlation coefficient >0.999 with the acceptable precision and accuracy (<2% relative standard deviation) for all four drugs. In conclusion, the method is simple and it does not require any pH adjustment of the buffer/mobile phase, and within 11 min, the separation of all four drugs can be achieved. Overall, the method is suitable for quality testing of fixed-dose combination tablets in limited-resource settings.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"821-828"},"PeriodicalIF":1.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140876568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Quality Evaluation of Qizhi Xiangfu Pills Based on Fingerprint with Chemometric Analysis and Quantitative Analysis of Multi-Components by Single Marker. 基于指纹化学计量分析和单标记多成分定量分析的杞菊地黄丸质量评价新方法。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-11-01 DOI: 10.1093/chromsci/bmae005

Xiaoli Xu, Lin Yang, Dezhang Zhao, Yiwu Wang, Lei Dai, Shuya Li, Dan He

{"title":"New Quality Evaluation of Qizhi Xiangfu Pills Based on Fingerprint with Chemometric Analysis and Quantitative Analysis of Multi-Components by Single Marker.","authors":"Xiaoli Xu, Lin Yang, Dezhang Zhao, Yiwu Wang, Lei Dai, Shuya Li, Dan He","doi":"10.1093/chromsci/bmae005","DOIUrl":"10.1093/chromsci/bmae005","url":null,"abstract":"Qizhi Xiangfu Pills (QZXFPs) is one of the most commonly used traditional Chinese medicine preparations for the treatment of dysmenorrhea, but the existing quality evaluation standards have certain shortcomings and deficiencies. An effective and scientific quality evaluation method plays a vital role in medication safety. In this study, fingerprint combined with chemometric analysis and quantitative analysis of multi-components by a single marker (QAMS) method was used to comprehensively evaluate the quality of QZXFPs. The fingerprints of 28 batches samples were established and 23 common peaks were distinguished, of which 7 peaks were identified as albiflorin, paeoniflorin, baicalin, ligustilide, cyperotundone, nootkatone and α-cyperone. The content of these seven active ingredients was determined simultaneously by the QAMS method and there was no significantly different between QAMS and the external standard method. Additionally, similarity analysis, hierarchical cluster analysis, principal component analysis and orthogonal partial least squares discrimination analysis were applied for classifying the 28 batches of samples, and to find the main components causing the quality differences between different batches. In conclusion, the established method can comprehensively evaluate the consistency of quality between different batches and provide a reference for formulation quality evaluation to ensure safe and effective application of QZXFPs.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"854-863"},"PeriodicalIF":1.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140049658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thiazolidinediones: Recent Development in Analytical Methodologies. 噻唑烷二酮类化合物：分析方法的最新发展。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-10-01 DOI: 10.1093/chromsci/bmad058

Tarang Patel, Vatsal Patel

{"title":"Thiazolidinediones: Recent Development in Analytical Methodologies.","authors":"Tarang Patel, Vatsal Patel","doi":"10.1093/chromsci/bmad058","DOIUrl":"10.1093/chromsci/bmad058","url":null,"abstract":"The instrumental analytical methods that have been developed and utilized for the determination of thiazolidinedione in bulk medications, formulations and biological fluids have been reviewed after an in-depth analysis of the literature published in a variety of analytical and pharmaceutical chemistry-related journals. The approaches covered by this research, which covers the years 2001-2022, include complex methods for analysis, chromatographic techniques and spectrometric analytical procedures. The mobile phase, flow rate, sample matrix, wavelength and other factors identified in the literature were just a few of the parameters used to evaluate thiazolidinediones. The present review focuses on the published analytical techniques for thiazolidinedione analysis that have been previously identified in the literature. The specified outcomes followed extensive learning, and the most recent advances in analytical methods for the identification of pioglitazone, pioglitazone HCl, rosiglitazone, rosiglitazone maleate and lobeglitazone were reviewed. Additionally, this article briefly discusses features of analytical discovery on thiazolidinediones, which will enable readers to access all discoveries in one place with precise outcomes.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"789-802"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10274299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Thiazolidinediones: Recent Development in Analytical Methodologies. 更正：噻唑烷二酮类化合物：分析方法的最新发展。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-10-01 DOI: 10.1093/chromsci/bmad080

引用次数: 0

Correction to: Solid-Phase Extraction (SPE) Technique to Quantify Cefdinir in Human Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). 更正：使用液相色谱-串联质谱（LC-MS/MS）技术对人血浆中的头孢地尼进行定量的固相萃取（SPE）技术。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-10-01 DOI: 10.1093/chromsci/bmad044

引用次数: 0

A Novel RP-HPLC Method for Simultaneous Estimation of Vilanterol Trifenatate, Umeclidinium Bromide and Fluticasone Furoate in Inhalation Dry Powder Formulation. 同时测定吸入干粉制剂中三苯甲酸维兰特罗、溴化乌美拉明和糠酸氟替卡松的新型 RP-HPLC 方法。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-10-01 DOI: 10.1093/chromsci/bmad075

Shweta Yadav, Vandana Jain, Hemant Magar, M Vijay Kumar, Sayali Warde, Raman Mohan Singh

{"title":"A Novel RP-HPLC Method for Simultaneous Estimation of Vilanterol Trifenatate, Umeclidinium Bromide and Fluticasone Furoate in Inhalation Dry Powder Formulation.","authors":"Shweta Yadav, Vandana Jain, Hemant Magar, M Vijay Kumar, Sayali Warde, Raman Mohan Singh","doi":"10.1093/chromsci/bmad075","DOIUrl":"10.1093/chromsci/bmad075","url":null,"abstract":"The dry powder inhalation formulation containing vilanterol trifenatate, umeclidinium bromide and fluticasone furoate intended for the therapy of bronchospasm related to chronic obstructive pulmonary disease and bronchial asthma was selected for the development and validation of a novel, selective, accurate, precise, quick and cost-efficient reversed-phase, high-performance liquid chromatography method. Neither an official monograph nor a single method has yet been published for the simultaneous estimation of these three compounds, which makes this method novel. The stationary phase of an ACE-C18-PFP column (250 mm × 4.6 mm, 5 μ) was used with a mobile phase of 25-mM sodium perchlorate buffer (pH 2.5 adjusted with ortho-phosphoric acid) and acetonitrile (40:60% v/v) at a flow rate of 1 mL/min to optimize chromatographic variables. The column temperature was kept at 40°C, and detection was at 224 nm, which was the isosbestic point of these three drugs. Well-resolved good peak symmetry was obtained for all three molecules by isocratic elution in less than 10 min, and the retention times of vilanterol trifenatate, umeclidinium bromide and fluticasone furoate were found to be 3.7, 5.4 and 8.3 min, respectively. The proposed method was validated as per ICH Q2 (R1) guidelines, and the calibration curves were linear in concentration ranges of 5-35 μg/mL for vilanterol trifenatate, 5-80 μg/mL for umeclidinium bromide and 5-150 μg/mL for fluticasone furoate, with mean % recoveries of 99-100%. The limits of detection and quantitation are 0.15 and 0.45 μg/mL for vilanterol trifenatate, 0.58 and 1.77 μg/mL for umeclidinium bromide and 0.32 and 0.96 μg/mL for fluticasone furoate, respectively. Hence, the proposed RP-HPLC technique was successfully used to quantify the inhalation formulation containing all three compounds.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"761-766"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10172968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0