Journal of chromatographic science最新文献

Simultaneous Determination of 8, 11, 12, 20-HETEs and 13 s-HODE in Paraquat and Diquat Poisoning Patients Via the UPLC-MS/MS Method. UPLC-MS/MS法同时测定百草枯和双菊枯中毒患者体内8、11、12、20-HETEs和13 s-HODE

IF 1.3 4区化学

Journal of chromatographic science Pub Date : 2025-10-07 DOI: 10.1093/chromsci/bmaf052

Zheng Yu, Xiaofang Ke, Minjie You, Tao Zhou, Jianhui Yang, Xianqin Wang, Congcong Wen, Xiuwei Shen, Xiuhua Zhang, Lufeng Hu

{"title":"Simultaneous Determination of 8, 11, 12, 20-HETEs and 13 s-HODE in Paraquat and Diquat Poisoning Patients Via the UPLC-MS/MS Method.","authors":"Zheng Yu, Xiaofang Ke, Minjie You, Tao Zhou, Jianhui Yang, Xianqin Wang, Congcong Wen, Xiuwei Shen, Xiuhua Zhang, Lufeng Hu","doi":"10.1093/chromsci/bmaf052","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf052","url":null,"abstract":"An Ultra Performance Liquid Chromatography - Mass Spectrometry/Mass Spectrometry (UPL-MS/MS) method for simultaneous determination of 8, 11, 12, 20- hydroxyeicosatetraenoic acids (HETEs) and 13 s-hydroxyoctadecadienoic acid (13 s-HODE) in plasma was developed and optimized. The chromatographic separation of 8, 11, 12, 20-HETEs and 13 s-HODE was achieved on a Peptide Bridged Ethylene Hybrid Particles (BEH) C18 column (2.1 mm × 150 mm, 1.7 μm) with mobile phase A (Water containing 0.1% formic acid) and mobile phase B (acetonitrile containing 0.1% formic acid). HETEs and 13 s-HODE were quantified using multiple reaction monitoring mode. The developed UPLC-MS/MS method was applied to determine the plasma samples of 32 paraquat (PQ) poisoning patients, 20 diquat (DQ) poisoning patients and 38 healthy subjects. The results showed that the 8, 11, 12, 20-HETEs and 13 s-HODE have good linearity (R2 > 0.99) from 0.1-500 ng/mL. The levels of HETEs and 13 s-HODE were dramatically increased in PQ and DQ poisoning patients. There were high correlation between 8, 11, 12, 20-HETEs and 13 s-HODE. Orthogonal partial least squares discrimination analysis showed that three groups were distributed in different areas and separated well. In conclusion, a sensitive UPLC-MS/MS method had been developed and validated for determination of 8, 11, 12, 20-HETEs and 13 s-HODE in plasma of PQ and DQ patients.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 9","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Neural Network-Assisted Natural Deep Eutectic Solvent Extraction for Enhanced Bioactive Yield from Folium Mori with Cytotoxicity Profiling. 遗传神经网络辅助天然深共熔溶剂萃取提高桑叶生物活性产率及细胞毒性分析。

IF 1.3 4区化学

Journal of chromatographic science Pub Date : 2025-08-30 DOI: 10.1093/chromsci/bmaf048

Yanxia Zhou, Jinglin Shen, Hongmei Chen, Ping Huang

引用次数: 0

A Simple and Highly Sensitive Liquid Chromatographic-Mass Spectrometric Method for the Determination of Itraconazole and Its Major Metabolite in Human Plasma and Its Application to a Bioequivalence Study. 高效液相色谱-质谱联用法测定人血浆中伊曲康唑及其主要代谢物及其生物等效性研究

IF 1.3 4区化学

Journal of chromatographic science Pub Date : 2025-08-30 DOI: 10.1093/chromsci/bmaf035

Hamed Hamishehkar, Rasoul Hosseinpour, Ladan Dayani, Bahareh Samii, Jaber Emami

{"title":"A Simple and Highly Sensitive Liquid Chromatographic-Mass Spectrometric Method for the Determination of Itraconazole and Its Major Metabolite in Human Plasma and Its Application to a Bioequivalence Study.","authors":"Hamed Hamishehkar, Rasoul Hosseinpour, Ladan Dayani, Bahareh Samii, Jaber Emami","doi":"10.1093/chromsci/bmaf035","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf035","url":null,"abstract":"Itraconazole is an oral triazole antimycotic drug. Bioequivalence studies are cornerstones for the approval of generic drug development globally. The present study describes a simple, sensitive and economical LC-MS/MS method for the determination of itraconazole and its metabolite in human plasma. Itraconazole (drug), hydroxyitraconazole (metabolite), and atorvastatin as internal standard were added to plasma samples. The standard curve of both the drug and metabolite covering the 4-320 ng/mL concentration range, was linear (R2 = 0.999). Also, a limit of quantification of 4 ng/mL of a sample size of 0.4 mL is achieved which is comparable or even better than the reported methods. The applicability of this method was proven by analyzing true samples obtained after the administration of 100 mg itraconazole of test (Noxifunge®) and reference (Sporanox®) to healthy volunteers. The 90% confidence intervals of the logarithmically transformed AUC0-72, AUC0-∞, and Cmax of the two formulations both for the drug and the metabolite are within the accepted levels proposed by FDA and EMA. Therefore, the presented method is suitable for bioavailability, pharmacokinetic, and bioequivalent studies in humans.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harmonized Approach of Design of Experiments with Green and White Analytical Chemistry in Unified Framework for Synchronous Chromatographic Assay of Diverse Combinations of Anti-diabetic Drugs in Pharmaceutical Dosage Forms. 药物剂型中不同组合抗糖尿病药物同步色谱分析统一框架中绿白分析化学实验设计的协调方法

IF 1.3 4区化学

Journal of chromatographic science Pub Date : 2025-08-30 DOI: 10.1093/chromsci/bmaf051

Pintu Prajapati, Bageshree Rana, Veerashakar Pulusu, Anzarul Haque, Shailesh Shah

{"title":"Harmonized Approach of Design of Experiments with Green and White Analytical Chemistry in Unified Framework for Synchronous Chromatographic Assay of Diverse Combinations of Anti-diabetic Drugs in Pharmaceutical Dosage Forms.","authors":"Pintu Prajapati, Bageshree Rana, Veerashakar Pulusu, Anzarul Haque, Shailesh Shah","doi":"10.1093/chromsci/bmaf051","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf051","url":null,"abstract":"Antidiabetic drugs, including metformin hydrochloride, are typically used to treat diabetes mellitus. Current methods for evaluating these drug combinations often involve the use of harmful solvents and specific conditions, leading to environmental risks and substantial toxic waste. White analytical chemistry proposes an innovative solution to this problem by developing environmentally friendly, cost-effective and user-friendly chromatographic methods. A synchronous chromatographic assay method for multiple combined pharmaceutical dosage forms of antidiabetic drugs was developed using a single set of chromatographic conditions and safe organic solvents. Method development was carefully carried out using a design of experiments approach to reduce organic solvent waste. The Plackett-Burman design was used in the screening phase to identify critical method variables and responses, facilitated by Minitab 18 software. The Box-Behnken design was then used to optimize these critical variables. A synchronous chromatographic assay of multiple combined pharmaceutical dosage forms was conducted under optimized chromatographic conditions. Finally, a thorough evaluation of the proposed and existing chromatographic methods was conducted using green and white analytical chemical metrics.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Bioanalytical Method Development and Validation of Navitoclax and Doxorubicin in Rat Plasma Using UHPLC-HESI-LTQ-MS. 用UHPLC-HESI-LTQ-MS建立大鼠血浆中纳维托昔和阿霉素的同时生物分析方法及验证。

IF 1.3 4区化学

Journal of chromatographic science Pub Date : 2025-08-30 DOI: 10.1093/chromsci/bmaf046

Nitesh Rai, Deepak Kumar, Parul Sharma, Pirangi Srikanth, Shaik Khaja Moinuddin, Pramod Kumar, Sukhendu Nandi

{"title":"Simultaneous Bioanalytical Method Development and Validation of Navitoclax and Doxorubicin in Rat Plasma Using UHPLC-HESI-LTQ-MS.","authors":"Nitesh Rai, Deepak Kumar, Parul Sharma, Pirangi Srikanth, Shaik Khaja Moinuddin, Pramod Kumar, Sukhendu Nandi","doi":"10.1093/chromsci/bmaf046","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf046","url":null,"abstract":"A simple, sensitive, and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was successfully developed and validated to determine navitoclax and doxorubicin in rat plasma. Ketoconazole and daunorubicin were employed as internal standards to ensure accurate quantification and method consistency. The sample preparation involved a straightforward protein precipitation technique, which facilitated efficient extraction of the analytes from the plasma matrix. The resulting supernatant was subjected to drying using a vacuum concentrator and later reconstituted before being injected into the LC-MS system. Separation was carried out using a SHIMADZU Shim-pack GIST C-18 column with mobile phase, consisting of a carefully balanced mixture of acetonitrile and water with 0.1% formic acid, with a flow rate of 0.5 mL/min. This composition ensured efficient elution and minimized matrix effects, contributing to the method's robustness and reproducibility. The developed method was comprehensively validated according to regulatory guidelines, assessing various parameters including specificity, selectivity, sensitivity, calibration curve performance, precision, accuracy, stability and dilution integrity. This bioanalytical method has the potential to be extended to various clinical settings, enabling the monitoring drug metabolism, monitoring potential drug-drug interactions and understanding the pharmacokinetic profile and adverse effect potential of the combination therapy.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Analysis of Cyproconazole and Tebuconazole in Suspension Concentrate Formulation by Reverse-Phase High-Performance Liquid Chromatography. 反相高效液相色谱法同时分析悬浮液中环丙康唑和戊康唑的含量。

IF 1.3 4区化学

Journal of chromatographic science Pub Date : 2025-08-30 DOI: 10.1093/chromsci/bmaf047

Sahadev Katam, Prasad Munipalli, Bhavani Balram, Vasu Govardhana Reddy Peddiahgari

引用次数: 0

Streamlined Sample Cleanup: Small Molecule Fractionation and Extraction Via Low-Volume Polymer Monolithic Columns for In-Line Analysis. 流线型样品清理：小分子分离和提取通过小体积聚合物整体柱进行在线分析。

IF 1.3 4区化学

Journal of chromatographic science Pub Date : 2025-08-30 DOI: 10.1093/chromsci/bmaf049

Ischa Bremer, Charles Clark, Bert Wouters, Amy Harms, Thomas Hankemeier

{"title":"Streamlined Sample Cleanup: Small Molecule Fractionation and Extraction Via Low-Volume Polymer Monolithic Columns for In-Line Analysis.","authors":"Ischa Bremer, Charles Clark, Bert Wouters, Amy Harms, Thomas Hankemeier","doi":"10.1093/chromsci/bmaf049","DOIUrl":"10.1093/chromsci/bmaf049","url":null,"abstract":"Polymer monoliths are stationary-phase materials for liquid chromatography and solid-phase extraction. Their porous structure, tuneability and simple synthesis enable tailoring to specific analysis requirements in analytical chemistry. Typically, polymer monoliths are used to separate larger biomolecules. Due to their lower binding capacity, the applications of polymer monoliths for the chromatographic separation of small molecules remain limited. However, recent literature has shown that polymer monoliths have the potential for the extraction of small molecules. In this research, butyl methacrylate-co-ethylene glycol dimethacrylate polymer monoliths were synthesized using localized UV polymerization in capillaries. The performance of reversed-phase polymer monoliths in automated in-line solid-phase extraction-mass spectrometry was demonstrated by the analysis of endocannabinoids from neat standard mixes and spiked cell culture media without prior sample preparation. The synthesized monoliths exhibited a binding capacity of 1896 pmol. Furthermore, we showed the repeatability of the monolith synthesis, with a variance in permeability of 19%. The system's stability is demonstrated through the analysis of multiple batches, comparing different monoliths and reusing the same monolith repeatedly, resulting in relative standard deviations (RSDs) below 20% for all extracted compounds. This automated method with hyphenated mass spectrometry improves throughput over previous manual monolithic extractions for small molecules.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 8","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Method for Simultaneous Determination of Amino Acid Surfactants Sodium N-Lauroylsarcosinate and Sodium Methyl Cocoyl Taurate in Personal Care Products, by Using High Performance Liquid Chromatography. 高效液相色谱法同时测定个人护理用品中氨基酸表面活性剂n -月桂酰肌氨酸钠和甲基椰油酰牛磺酸钠的含量。

IF 1.3 4区化学

Journal of chromatographic science Pub Date : 2025-07-24 DOI: 10.1093/chromsci/bmaf044

Kumar Thangarathinam, Udhayachandran Narayanan

引用次数: 0

Optimized Simultaneous Estimation of Metformin, Teneligliptin, and Pioglitazone in Tablet and Bulk Drug: A Box-Behnken Design Approach for RP-UFLC Method Development and Validation. 片剂和原料药中二甲双胍、替尼格列汀和吡格列酮含量的优化同时估计：RP-UFLC方法开发与验证的Box-Behnken设计方法

IF 1.3 4区化学

Journal of chromatographic science Pub Date : 2025-07-24 DOI: 10.1093/chromsci/bmaf045

Jignesh Panchal, Jayesh Dhalani

{"title":"Optimized Simultaneous Estimation of Metformin, Teneligliptin, and Pioglitazone in Tablet and Bulk Drug: A Box-Behnken Design Approach for RP-UFLC Method Development and Validation.","authors":"Jignesh Panchal, Jayesh Dhalani","doi":"10.1093/chromsci/bmaf045","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf045","url":null,"abstract":"The combination of metformin (MET), teneligliptin (TEN), and pioglitazone (PIO) is newest fixed-dose combination for the treatment type 2 diabetes launched in India. An accurate, rapid, and cost-effective reversed phase ultra-fast liquid chromatography method was developed, validated, and applied for the quantification of MET, TEN, and PIO in bulk and tablet with a very short runtime. Box-Behnken design was implemented to optimize the ultra-fast liquid chromatography conditions. A C18 column (150 × 4.6 mm, 5μ) was utilized with a mobile phase buffer (0.01 M phosphate buffer, pH = 6.2) and acetonitrile in a 51:49 ratio with flow rate of 1.5mL/min at column oven temperature 40°C. Detection was carried out at 255nm by 20 μL injection volume. Retention times were found to be 0.94, 1.36, and 2.07 min, whereas the limit of quantification were 0.209, 0.712, and 57.030 μg/mL for MET, TEN, and PIO, respectively. The linearity of anticipated development was studied in the range of 250-1250 μg/mL (MET, r2 = 0.99974), 10-50 μg/mL (TEN, r2 = 0.99997), and 7.5-37.5μg/mL (PIO, r2 = 0.99999). The relative standard deviation values for method and intermediate precision were <2%. The method was validated as per International Conference on Harmonization guideline for accuracy, precision, linearity, limit of detection, limit of quantification, specificity, robustness, and forced degradation. This is the only study that presents reversed phase ultra-fast liquid chromatography method for the new fixed-dose combination of MET, TEN, and PIO, with the shortest run time of 3.0 min. The proposed method is innovative, efficient, precise, quickest, and economical with high accuracy which makes the study novel.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 7","pages":""},"PeriodicalIF":1.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening Method for the Determination of per- and Polyfluoroalkyl Substances (PFAS) in Textiles by Combustion and Ion Chromatography. 燃烧和离子色谱法测定纺织品中全氟烷基和多氟烷基物质的筛选方法。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-07-24 DOI: 10.1093/chromsci/bmaf043

Lihua Cao, Youchao Ding, Kai Qian, Xiangxiang Wang, Xiaoqiong Wang, Jia Zhou, Juan Tang

{"title":"Screening Method for the Determination of per- and Polyfluoroalkyl Substances (PFAS) in Textiles by Combustion and Ion Chromatography.","authors":"Lihua Cao, Youchao Ding, Kai Qian, Xiangxiang Wang, Xiaoqiong Wang, Jia Zhou, Juan Tang","doi":"10.1093/chromsci/bmaf043","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf043","url":null,"abstract":"A screening method was developed for the determination of per- and polyfluoroalkyl substances in textiles by combustion and ion chromatography. In this work, a new type of high-temperature combustion absorption device was designed, and the samples were burned, cracked and gasified by temperature programmed heating mode. The produced free fluorine and hydrogen fluoride gases were absorbed by water vapor and completely transformed into inorganic fluoride anion. After condensation and collection, it was separated and determined by ion chromatography. The pre-treatment conditions were optimized including the sample weight, the combustion method, the combustion gas and its flow rate, the water evaporation rate and the condensate collection method. Method validation was performed in negative samples of cotton, wool and polyester, at spiked levels of 5, 50 and 200 mg/kg, respectively. The average recoveries were between 85.7 and 95.0% with relative standard deviations <20% (n = 7) indicating satisfactory accuracy and repeatability. The calibration curve was performed with the correlation coefficients (R2) higher than 0.999 within a linearity range of 0.01-2.0 mg/L. The limit of quantification was 5.0 mg/kg. The developed method was successfully used to determine the total fluorine in market samples. It can meet the screening determination of PFAS in various textiles.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 7","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0