Journal of chromatographic science最新文献

Verifying the Equivalence of Hawthorn Leaves Standard Decoction and Formula Granules by LC-MS and Oxidative Stress Test. 用LC-MS和氧化应激试验验证山楂叶标准汤和配方颗粒的等效性。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-02-08 DOI: 10.1093/chromsci/bmad089

Ling-Di Wang, Jing-Yun Gao, Li-Ying Duan, Hai-Feng Pan

{"title":"Verifying the Equivalence of Hawthorn Leaves Standard Decoction and Formula Granules by LC-MS and Oxidative Stress Test.","authors":"Ling-Di Wang, Jing-Yun Gao, Li-Ying Duan, Hai-Feng Pan","doi":"10.1093/chromsci/bmad089","DOIUrl":"10.1093/chromsci/bmad089","url":null,"abstract":"Objective: To verify the equivalence of hawthorn leaves standard decoction and formula granules.Methods: In this experiment, liquid chromatograph mass spectrometer (LC-MS) was used to examine the chemical composition of hawthorn leaves standard decoction and formula granules, separately. In addition, oxidative stress test was used to explore the antioxidant capacity of them.Results: 71 chemical components were identified by LC-MS. Among them, 64 and 56 compounds were identified in the standard decoction and formula granules, respectively. There were a total of 49 common components, with no significant difference in content. Oxidative stress test showed that hawthorn leaves standard decoction and formula granules had no obvious toxicity to human umbilical vein endothelial cells. Compared with the model group, the same dose of hawthorn leaves formula granule and standard decoction could inhibit the secretion of lactate dehydrogenase and malondialdehyde (P < 0.05), and increase the content of superoxide dismutase (P < 0.01), with no statistically significant difference.Conclusions: There is no significant difference in the main active ingredients between the standard decoction and the formula granules, and the antioxidant activity in vitro is equivalent, providing an important theoretical basis for the further development of hawthorn leaves formula granules.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138482424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of Pharmacokinetics for Ivermectin B1a from Beagle Dogs. 比格犬伊维菌素 B1a 的药代动力学研究

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-02-08 DOI: 10.1093/chromsci/bmad092

Yuyang Chen, Xiaofang Huang, Zizheng Guo, Jingyu Zhang, Lixin Zhang, Renke Dai

{"title":"Study of Pharmacokinetics for Ivermectin B1a from Beagle Dogs.","authors":"Yuyang Chen, Xiaofang Huang, Zizheng Guo, Jingyu Zhang, Lixin Zhang, Renke Dai","doi":"10.1093/chromsci/bmad092","DOIUrl":"10.1093/chromsci/bmad092","url":null,"abstract":"Ivermectin has been widely used for antiparasitic drug, and has recently shown a broad-spectrum antiviral activity, including anti-Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the pharmacokinetic property of ivermectin has not been fully investigated yet. During the plasma preparation, ~32-46% of ivermectin was found in the precipitation. An Liquid Chromatograph-Mass Spectrometer (LC-MS/MS) method for ivermectin in the whole blood samples from beagle dogs was developed and validated. The specificity, accuracy, precision (intra-day and inter-day), matrix effect, recovery and stability of analyte reported here are satisfied with the criteria of Food and Drug Administration (FDA)-Bioanalysis guideline. The oral administrations pharmacokinetics of ivermectin in beagle dogs under fasting and after high-fat meal were studied, and the following parameters were obtained: fasting Cmax, 104 ± 35 μg·L-1; area under the concentration-time curve (AUC0-∞), 2,555 ± 941 h·μg·L-1; and high-fat meal Cmax, 147 ± 35 μg·L-1; AUC0-∞, 4,198 ± 1,279 h·μg·L-1. When the P-gp inhibitor curcumin was also coadministrated orally, Cmax and AUC0-∞ were found to be 177 ± 57 and 4,213 ± 948 h·μg·L-1, respectively. With the comparison to fasting treatment, coadministration of P-gp inhibitor curcumin resulted in increase of the exposure of ivermectin by 1.6-fold, while the exposure after the high-fat diet versus fasting was increased approximately in 1.4-fold, indicating that alternative absorption might play an important role for increasing the exposure of ivermectin for future clinic applications.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138885095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Analytical Approach by Tri-Combination of Gradient UFLC, Response Surface Methodology and Green Chemistry Principle for Simultaneous Quantification of Azelnidipine and Chlorthalidone in Rabbit Plasma. 梯度超高效液相色谱法、响应面法和绿色化学原理三结合分析法用于兔血浆中阿折地平与氯沙坦酮的同时定量分析

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-02-08 DOI: 10.1093/chromsci/bmae032

C Bhagyalakshmi, T N Rekha, Piyongsola Longkumer, Koushik Nandan Dutta, Bhargab Jyoti Sahariah, B Ramesh, Manish Majumder

{"title":"An Analytical Approach by Tri-Combination of Gradient UFLC, Response Surface Methodology and Green Chemistry Principle for Simultaneous Quantification of Azelnidipine and Chlorthalidone in Rabbit Plasma.","authors":"C Bhagyalakshmi, T N Rekha, Piyongsola Longkumer, Koushik Nandan Dutta, Bhargab Jyoti Sahariah, B Ramesh, Manish Majumder","doi":"10.1093/chromsci/bmae032","DOIUrl":"10.1093/chromsci/bmae032","url":null,"abstract":"In this study, a sustainable and eco-friendly method is developed to quantify azelnidipine and chlorthalidone in rabbit plasma by gradient liquid chromatography based on green chemistry principle and analytical quality by design. The separation was achieved on a Shim pack C18 (25 cm × 5 cm × 4.6 μm) column with L1 packing. The mobile phase compromised of ethanol and 50-Mm ammonium acetate buffer (pH.6) at flow rate of 0.6 mL/min with 25-min runtime. The resolution and asymmetric factor were identified as critical analytical attributes (CAAs). The screening studies employing Control Noise Experimentation revealed that mobile phase pH, flow rate and ethanol concentration at 6 and 15 min significantly affected the CAAs method. The critical method parameters were optimized using Central Composition design. Chromatogram showed peak of the drugs at retention time of 9.03 min for chlorthalidone and 16.83 min for azelnidipine. The greenness score of the analytical method was found to be 1876.43 using analytical method greenness score calculator. The validation of the developed method was done which showed linearity at the range of 16-520 ng/mL, with R2 of 0.9992 and 0.9996 for azelnidipine and chlorthalidone, respectively, furthermore accuracy, precision, recovery and stability studies are carried out.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141076129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecularly imprinted polymer for solid-phase extraction of oleanolic acid from Ligustrum lucidum fruit.

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-02-08 DOI: 10.1093/chromsci/bmaf010

Jianling Xu, Wenna Wang, Yixin Chen, Xiaotian Xu, Ao Duan, Yongyan Zhu, Quanhong Zhu

{"title":"Molecularly imprinted polymer for solid-phase extraction of oleanolic acid from Ligustrum lucidum fruit.","authors":"Jianling Xu, Wenna Wang, Yixin Chen, Xiaotian Xu, Ao Duan, Yongyan Zhu, Quanhong Zhu","doi":"10.1093/chromsci/bmaf010","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf010","url":null,"abstract":"A molecularly imprinted polymer (MIP) was synthesized for the selective extraction of oleanolic acid (OA), employing OA as the template molecule, acrylamide as the functional monomer, ethylene glycol dimethacrylate as the cross-linking agent, azobisisobutyronitrile as the initiator, and chloroform as the porogenic solvent. The characterization of the obtained MIPs was evaluated by scanning electron microscopy, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller (BET) analysis and X-ray photoelectron spectroscopy. The MIPs reached adsorption equilibrium within 2 hours to OA, with high adsorption capacity of 124.68 mg/g. Subsequently, these MIPs were packed in empty solid-phase extraction (SPE) cartridge to enrichment OA from Ligustrum lucidum fruit extract. The parameters of SPE were optimized as follows: loading 0.5 ml of a 1.0 mg/ml OA reference solution, washing with 0.5 ml of toluene, and eluting with 4 ml of methanol. Under these conditions, the enrichment rate of OA from L. lucidum extract reached 76.0%. Additionally, an high-performance liquid chromatography method for determining OA content was validated. This study provides an effective approach for the separation and enrichment of OA from complex matrices and also provides practical feasibility for the separation and enrichment of other triterpenoids.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Problems in Commercial Kits of 25-Hydroxy Vitamin D and the Development of Simple, Robust and Faster HPLC Method. 25-Hydroxy Vitamin D 商用试剂盒中存在的问题以及简单、稳健、快速的 HPLC 方法的开发。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-02-08 DOI: 10.1093/chromsci/bmae042

Syed Muhammad Saad, Abdul Rafiq Khan, Khalid Mohammed Khan, Ali Al-Othaim

{"title":"Problems in Commercial Kits of 25-Hydroxy Vitamin D and the Development of Simple, Robust and Faster HPLC Method.","authors":"Syed Muhammad Saad, Abdul Rafiq Khan, Khalid Mohammed Khan, Ali Al-Othaim","doi":"10.1093/chromsci/bmae042","DOIUrl":"10.1093/chromsci/bmae042","url":null,"abstract":"Vitamin D is a lipid-soluble compound that plays a key role in bone mineral metabolism. The commercial current kits and several published assay methods (High-performance liquid chromatography (HPLC) and Immunoassay) are complicated due to the use of multiple reagents, larger sample volume, high backpressure, longer extraction time, evaporation under nitrogen after extraction, significant interference and antibody cross-reactivity. Here we report a new HPLC method for the determination of 25-hydroxyvitamin D2 (25-OHD2) and 25-hydroxyvitamin D3 (25-OHD3) that is simple (no evaporation), rapid (10-minute run time) and robust. Serum sample (300 μl) is mixed with 300 μl acetonitrile containing lauraphenone as internal standard. After vortexing and centrifugation, the supernatant was loaded into C18 extraction cartridges, washed with 70% methanol and then eluted with 200 μl of a mixture of 70% ethanol and 30% isopropyl alcohol (IPA). The eluent was mixed with 50 μl of water and injected into the HPLC-UV system for analysis. The method proved to be linear in the range of 10-750 nmol/L of 25-OHD2 and 25-OHD3. The intra- and inter-assay precision was less than 10 for both compounds at four different concentrations. The method was compared with (LC-MS/MS) and the correlation coefficients (R2) were 0.9454 and 0.9673 for 25-OHD2 and 25-OHD3 respectively. The proposed HPLC method is simple, rapid, robust and free from the most common problems encountered with commercial kits. It can be used in a high-volume laboratory that uses the HPLC method for the simultaneous determination of 25-OHD2 and 25-OHD3 in serum samples.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Simultaneous HPTLC Method and Validation for the Quality Assessment of Ayurvedic Formulation-Ayush Kvatha Churna by Using Marker Compound Rosmarinic Acid, Trans-Cinnamaldehyde and Piperine. 利用标记化合物香豆素酸、反式肉桂醛和胡椒碱，为阿育吠陀配方--Ayush Kvatha Churna 的质量评估开发同步 HPTLC 方法并进行验证。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-02-08 DOI: 10.1093/chromsci/bmae019

Umakant Sahu, Nagendra Singh Chauhan, Arun Kumar Singh Parihar, Kamleshwar Singh Karbhal, Shrikant R Inchulkar, Prashant Kumar Gupta, Rajesh Kumar Singh

{"title":"Development of Simultaneous HPTLC Method and Validation for the Quality Assessment of Ayurvedic Formulation-Ayush Kvatha Churna by Using Marker Compound Rosmarinic Acid, Trans-Cinnamaldehyde and Piperine.","authors":"Umakant Sahu, Nagendra Singh Chauhan, Arun Kumar Singh Parihar, Kamleshwar Singh Karbhal, Shrikant R Inchulkar, Prashant Kumar Gupta, Rajesh Kumar Singh","doi":"10.1093/chromsci/bmae019","DOIUrl":"10.1093/chromsci/bmae019","url":null,"abstract":"Ayurveda emphasizes the propagation of nature in maintaining health. In the present scenario, we have seen the faith of people in herbal drugs during the Covid 19 outbreak. The raises in the number of peoples have been using herbal drugs to boost immunity against infectious diseases shows the popularity of this ancient system of medicine. The standardization of Ayush Kvatha Churna (AKC), work set out to establish a straightforward, accurate and sensitive HPTLC method for the identification and quantification of marker compounds. The Rosmarinic acid, trans-Cinnamaldehyde and Piperine were used for the estimation of markers in Ayush Kvatha Churna by using HPTLC with a solvent system, consisting of Toluene: Ethyl acetate: Ethyl alcohol: Formic acid (5.6:2.4:2: 0.3 v/v/v/v). The Rf value 0.33 for Rosmarinic Acid, 0.69 for Piperine and 0.77 for trans-Cinnamaldehyde was observed and it is exactly complying with the corresponding bands in Ayush Kvatha Churna. The technique has been effectively verified and validated, enabling it to be used for the standardization or quantitative analysis of Rosmarinic acid, trans-Cinnamaldehyde and piperine in Ayush Kvatha Churna.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Chemical Constituents and Metabolites in Rat Plasma after Oral Administration of Zhi-Zi-Hou-Po Decoction by High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry.

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-02-08 DOI: 10.1093/chromsci/bmaf012

Jianyi Shi, Jiao Guan, Xu Pan, Bo Feng, Heyun Zhu

{"title":"Characterization of Chemical Constituents and Metabolites in Rat Plasma after Oral Administration of Zhi-Zi-Hou-Po Decoction by High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry.","authors":"Jianyi Shi, Jiao Guan, Xu Pan, Bo Feng, Heyun Zhu","doi":"10.1093/chromsci/bmaf012","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf012","url":null,"abstract":"Zhi-Zi-Hou-Po decoction (ZZHPD), consisting of Gardenia jasminoides Ellis, Magnolia officinalis cortex and Citrus aurantium L., is a classic traditional Chinese medicine prescription for the treatment of depression. However, the therapeutic material foundation of ZZHPD is still not very clear. In the present study, a high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was developed to simultaneously characterize and identify the chemical constituents and metabolites of ZZHPD in rat plasma after oral administration for the first time. As a result, a total of 88 compounds including 12 iridoid glycosides, eight lignans, 37 flavonoids, eight coumarins, seven monoterpenoids, four crocetin, three organic acids, one alkaloid and eight other compounds were identified or tentatively characterized. After intragastric administration of ZZHPD to rats, 40 compounds were detected in rat plasma, including 29 prototype constituents and 11 metabolites. Results showed that glucuronidation and sulfation were the main metabolic pathways of ZZHPD in rats. The analytical method established in this study provides an effective method for the study of the chemical composition of ZZHPD in vitro and in vivo, and provides an important basis for the study of the pharmacodynamic material basis and mechanism of action of ZZHPD.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Validated High-Performance Thin-Layer Chromatography Technique for Routine Analysis of Curcumin in Four Different Species of Curcuma Viz. C. amada, C. caesia, C. longa and C. zedoaria. 高效薄层色谱法测定四种姜黄中姜黄素的含量

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-02-08 DOI: 10.1093/chromsci/bmad063

Avinash Gangal, Manisha Duseja, Neeraj K Sethiya, Dheeraj Bisht, Sushil Kumar Chaudhary, Vijay Singh Rana

{"title":"A Validated High-Performance Thin-Layer Chromatography Technique for Routine Analysis of Curcumin in Four Different Species of Curcuma Viz. C. amada, C. caesia, C. longa and C. zedoaria.","authors":"Avinash Gangal, Manisha Duseja, Neeraj K Sethiya, Dheeraj Bisht, Sushil Kumar Chaudhary, Vijay Singh Rana","doi":"10.1093/chromsci/bmad063","DOIUrl":"10.1093/chromsci/bmad063","url":null,"abstract":"In this study, we investigated a new, simple, sensitive, selective and precise high-performance thin-layer chromatography (HPTLC) fingerprint and quantitative estimation method for the routine analysis of curcumin in Curcuma species viz. Curcuma amada, Curcuma caesia, Curcuma longa and Curcuma zedoaria. Linear ascending development was carried out in a twin-trough glass chamber saturated with toluene:acetic acid (4:1; v/v with 20 minutes of saturation). The plate was dried and analyzed by CAMAG TLC scanner III at white light and 366 nm. The system was found to give compact spots for curcumin (Rf 0.42). The relationship between the concentration of standard solutions and the peak response is linear within the concentration range of 10-70 ng/spot for curcumin. In result, curcumin was not detected in any of C. caesia extracts. The percentage of curcumin was found between 0.042 and 4.908 (%w/w) in different Curcuma species obtained by two different extraction methods viz. Soxhlet and sonication, respectively. Further, extraction via Soxhlet method is most suitable method to get higher curcumin content from rhizomes. The proposed HPTLC method may be use for routine quality testing and quantification of curcumin in Curcuma samples.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10022099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Detection of Food-Derived Agricultural Chemicals Residues in Waste Wool Fibers by Ultrasound-Assisted Extraction-QuEChERS Cleanup-UPLC-MS/MS. 利用超声辅助萃取-QuEChERS净化-UPLC-MS/MS首次检测废羊毛纤维中的食品衍生农用化学品残留。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-02-08 DOI: 10.1093/chromsci/bmae033

Yingao Li, Jun Liu, Shuai Li, Huidan Deng, Hui Zhao, Lili Meng, Lixia Jia

{"title":"First Detection of Food-Derived Agricultural Chemicals Residues in Waste Wool Fibers by Ultrasound-Assisted Extraction-QuEChERS Cleanup-UPLC-MS/MS.","authors":"Yingao Li, Jun Liu, Shuai Li, Huidan Deng, Hui Zhao, Lili Meng, Lixia Jia","doi":"10.1093/chromsci/bmae033","DOIUrl":"10.1093/chromsci/bmae033","url":null,"abstract":"Food-derived agricultural chemical residues (FACRs) accumulate gradually in organisms and can damage their nervous system, endocrine system and reproductive system, posing significant harm. Currently, there is little literature on the detection of FACRs in waste wool fibers. In this paper, an ultrasound-assisted extraction-QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) cleanup-UPLC-Ms/Ms method was applied for the qualitative analysis and quantitative determination of trace FACRs in waste wool fibers with 0.2% formic acid-methanol as extraction solvent and multi-selective ion scanning. Using the external standard method, it was shown that the 13 target FACRs showed good linearity in the mass concentration range of 0.1-50 μg/kg. The limits of detection were 1.0- 10.0 μg/kg and the limits of quantification were 4.0-40.0 μg/kg. The recoveries of the 13 target FACRs ranged from 78 to 112.6% at the 5-, 10- and 20-fold detection limit spiked levels, and the intra- or inter-day relative standard deviations were 2.05-6.98% or 1.98-6.99%, respectively. This method satisfied the detection requirements and can be used in applications.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141173944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical Methods for Estimating Metformin Hydrochloride, Pioglitazone and Teneligliptin in Diabetes Management: a Comprehensive Review.

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-02-08 DOI: 10.1093/chromsci/bmaf013

Sejal Pandya, Hitesh J Vekaria

{"title":"Analytical Methods for Estimating Metformin Hydrochloride, Pioglitazone and Teneligliptin in Diabetes Management: a Comprehensive Review.","authors":"Sejal Pandya, Hitesh J Vekaria","doi":"10.1093/chromsci/bmaf013","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf013","url":null,"abstract":"Diabetes mellitus remains a global health challenge, with a growing need for effective therapies. Metformin, Pioglitazone and Teneligliptin are widely prescribed medications for diabetes management. Accurate and precise estimation of these drugs is essential to ensure optimal therapeutic efficacy while minimizing side effects. This comprehensive review examines a multitude of analytical methods employed for the quantification of Metformin, Pioglitazone and Teneligliptin individually as well as in combine pharmaceutical dosage form focusing on their strengths, limitations and applicability within the realm of diabetes treatment. The choice of analytical method depends on various factors, such as the specific formulation, sample matrix complexity and the required sensitivity and quantification range. This review encompasses a wide range of analytical techniques, including spectroscopic methods (ultraviolet-visible, Fourier transform infrared and nuclear magnetic resonance spectroscopy), chromatographic methods (high-performance liquid chromatography, ultra-high performance liquid chromatography, and gas chromatography, high-performance thin-layer chromatography, liquid chromatography-tandem mass spectrometry), electrochemical approaches and emerging technologies like mass spectrometry and biosensors. Additionally, it delves into the intricacies of method development and validation, sample preparation and the importance of stability-indicating methods. Recent advancements in sample extraction and preparation techniques are explored to enhance sensitivity and accuracy. By offering a comprehensive assessment of the analytical methods used for the estimation of Metformin, Pioglitazone and Teneligliptin for diabetes treatment, this review serves as a valuable resource for researchers, clinicians and pharmaceutical scientists. It aids in selecting the most suitable analytical method for specific applications, contributing to the continuous improvement of diabetes management and treatment.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 3","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0