Journal of chromatographic science最新文献_第2页

A Validated Chiral Chromatography Method for Enantiomeric Separation of Pomalidomide in Human Plasma. 用于分离人血浆中泊马度胺对映体的经过验证的手性色谱法

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-08-20 DOI: 10.1093/chromsci/bmae026

Gyan Vardhan, Vikas Kumar, Puran Lal Sahu, Anuj Prakash, Ramasare Prasad, Shailendra Handu, Uttam Kumar Nath, Puneet Dhamija

{"title":"A Validated Chiral Chromatography Method for Enantiomeric Separation of Pomalidomide in Human Plasma.","authors":"Gyan Vardhan, Vikas Kumar, Puran Lal Sahu, Anuj Prakash, Ramasare Prasad, Shailendra Handu, Uttam Kumar Nath, Puneet Dhamija","doi":"10.1093/chromsci/bmae026","DOIUrl":"10.1093/chromsci/bmae026","url":null,"abstract":"In the present work, new chiral stationary phase high-performance liquid chromatography (CSP-HPLC) method was established and validated for the quantification of pomalidomide (PMD) enantiomers in human plasma. The chromatographic enantiomeric separation was achieved on a Daicel-CSP, Chiralpack IA 4.6 × 250 mm, 5 μm; because of its advantages of high degree of retention, high resolution capacity, better reproducibility, ability to produce lower back pressure and low degree of tailing. The mobile phase was maintained as methanol: glacial acetic acid (499.50 ml:50 μL). Ultraviolet wavelength for detection was 220 nm. PMD enantiomer-I and enantiomer-II were separated at 8.83 and 15.34 min, respectively. Limit of detection and limit of quantification for each enantiomer and the calibration curve of standard PMD was linear in range between 10-5,000 ng mL-1. The method was validated according to The International Council for Harmonisation of Technical Requirements of Pharmaceuticals for Human Use (ICH(Q2R1)) specific guidelines. We found no interference peak with PMD chromatogram obtained. This is a simple, reliable and specific method for detection and quantification of enantiomer of PMD in human plasma sample.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Volatile Halogenated Hydrocarbons in Drinking and Environmental Waters by Headspace Gas Chromatography. 利用顶空气相色谱法测定饮用水和环境水体中的挥发性卤代烃。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-08-09 DOI: 10.1093/chromsci/bmae047

Weifei Mo, Hongmei Hu, Jiangmei Yu, Tongtong Zhang, Qin Liu, Mengyan Li, Xiaoning Zhang, Tiejun Li, Yuanming Guo

{"title":"Determination of Volatile Halogenated Hydrocarbons in Drinking and Environmental Waters by Headspace Gas Chromatography.","authors":"Weifei Mo, Hongmei Hu, Jiangmei Yu, Tongtong Zhang, Qin Liu, Mengyan Li, Xiaoning Zhang, Tiejun Li, Yuanming Guo","doi":"10.1093/chromsci/bmae047","DOIUrl":"https://doi.org/10.1093/chromsci/bmae047","url":null,"abstract":"Volatile halogenated hydrocarbons (VHHs) are annually produced and released into the environment, posing a threat to public health. In this study, a simple, rapid, sensitive and automated method based on headspace and gas chromatography (GC) with electron-capture detection was described for the determination of VHHs in different concentration levels in water samples. The proposed headspace GC method was initially optimized, and the optimum experimental conditions found were 10-mL water sample containing 20% w/v sodium chloride placed in a 20-mL vial and stirred at 60°C for 35 min, and then 14 VHHs were well separated on DB-35 MS capillary column with a split ratio of 12.5: 1. The limits of detection were in the low μg/L level, ranging between 0.01 and 0.6 μg/L. Finally optimized method was applied for determination 14 VHHs in drinking and environmental waters. The total mean concentrations of VHHs were 34.962, 26.183, 3.228 and 647.344 μg/L in tap water, purified water with 1-year-old filter element, seawater and effluents, respectively. However, no VHHs was detected in purified water with a new filter element. The main composition is different among different water matrix, which may be attributed to their different sources.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoparticle Assisted Fabric Phase Sorptive Extraction for Azo Dye Determination in the Industrial Sewage. 纳米粒子辅助织物相吸附萃取法测定工业污水中的偶氮染料。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-08-06 DOI: 10.1093/chromsci/bmae046

Nayereh Rahimian, Javad Feizy, Zarrin Es'haghi

{"title":"Nanoparticle Assisted Fabric Phase Sorptive Extraction for Azo Dye Determination in the Industrial Sewage.","authors":"Nayereh Rahimian, Javad Feizy, Zarrin Es'haghi","doi":"10.1093/chromsci/bmae046","DOIUrl":"https://doi.org/10.1093/chromsci/bmae046","url":null,"abstract":"Currently, one of the significant environmental problems is the presence of azo dye materials in water sources. In this study, for the first time, a fast and sensitive sample preparation approach using nanoparticle-assisted fabric phase sorptive extraction (NFPSE) followed by high-performance liquid chromatography was examined to remove some azo dyes such as methyl red and sunset yellow from aqueous solutions. Primarily, the significance of several parameters affecting NFPSE, such as fabric type, the kind of sorbent, the number of contacts with sol-gel and the time of contact, was investigated. In addition, experiments were performed to determine the effect of different adsorption parameters, such as sample volume, adsorption time, adsorbent value, desorption time, ionic strength and pH. It was found that the calibration curve was linear within two ranges of concentrations (0.05-0.1 and 0.5-15 ng/L for methyl red; 0.05-0.5 and 0.5-15 ng/L for sunset yellow) with correlation coefficients better than 0.9683. The limit of detection was 0.014 ng/L for methyl red and 0.015 ng/L for sunset yellow. Repeatability Relative Standard Deviation (RSD) with three replicated experiments was 1.5-10% for methyl red and 2.5-5.8% for sunset yellow. Relative recovery percentages of 88-96% for methyl red and 62-92% for sunset yellow were obtained in the samples. Moreover, the results have shown that acceptable accuracy, precision and linearity make the \"fabric phase sorptive extraction\" a proper method for the determination of dyes from industrial sewage samples.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Simple HPLC-UV Method for Ivosidenib Determination in Human Plasma. 高效液相色谱-紫外分光光度法测定人血浆中艾沃西替尼的含量。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-07-10 DOI: 10.1093/chromsci/bmad082

Yoshito Gando, Takeo Yasu

{"title":"A Simple HPLC-UV Method for Ivosidenib Determination in Human Plasma.","authors":"Yoshito Gando, Takeo Yasu","doi":"10.1093/chromsci/bmad082","DOIUrl":"10.1093/chromsci/bmad082","url":null,"abstract":"Ivosidenib is used for the treatment of acute myeloid leukemia (AML) with isocitrate dehydrogenase 1 (IDH1) mutations. However, increased blood concentrations of ivosidenib are associated with a risk of a prolonged QT interval in patients with AML. Therapeutic drug monitoring in patients with AML with IDH1 mutation offers the potential to improve treatment efficacy while minimizing toxicity. In this study, we developed an efficient high-performance liquid chromatography-ultraviolet (HPLC-UV) method for the quantification of ivosidenib in plasma. Human plasma samples (50 μL) were processed by protein precipitation using acetonitrile, followed by chromatographic separation on a reversed-phase column with an isocratic mobile phase of 0.5% KH₂PO₄ (pH 4.5) and acetonitrile (45:55, v/v) at a flow rate of 1.0 mL/min, with ultraviolet detection at 245 nm. Calibration curves were linear over the range of 0.25-20 μg/mL with a coefficient of determination (r2) of 0.99999. Intra-day and inter-day precision were 1.20-8.04% and 0.69-4.20%, respectively. The assay accuracy was -2.00% to 1.93% and recovery was >91.2%. These findings support the effectiveness of the newly developed HPLC-UV method for the quantification of ivosidenib in human plasma. This simple and cost-effective method is expected to expand ivosidenib monitoring in laboratories lacking LC-MS/MS instruments.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49690731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecofriendly Chromatographic Assay of Metformin Combinations; Content Uniformity Tests and Toxicity Profiling. 二甲双胍复方制剂的生态友好型色谱分析；含量均匀性测试和毒性分析。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-07-10 DOI: 10.1093/chromsci/bmad026

Aml A Emam, Eglal A Abdelaleem, Fatma Magdy, Ahmed M Gouda, Fatma F Abdallah

{"title":"Ecofriendly Chromatographic Assay of Metformin Combinations; Content Uniformity Tests and Toxicity Profiling.","authors":"Aml A Emam, Eglal A Abdelaleem, Fatma Magdy, Ahmed M Gouda, Fatma F Abdallah","doi":"10.1093/chromsci/bmad026","DOIUrl":"10.1093/chromsci/bmad026","url":null,"abstract":"Accurate, sensitive and green HPTLC chromatographic method was proposed for simultaneous determination of metformin, glipizide and sitagliptin in the presence of metformin potential toxic impurities melamine and cyanoguanidine. The separation was completed on silica gel HPTLC F254 plates using a mixture of ethyl acetate: methanol: ammonia: formic acid (7: 2: 0.2: 0.2, by volume) as a developing system with UV scanning for the developed bands at 235 nm. The Rf values for metformin, glipizide, sitagliptin, melamine and cyanoguanidine were 0.17, 0.84, 0.67, 0.47 and 0.75, respectively. Linear responses were observed in the ranges of 0.2-3, 0.07-1.5, 1.5-5, 0.8-4 and 0.4-2 μg/band with correlation coefficients of 0.9999, 0.9998, 0.9997, 0.9996 and 0.9998 for metformin, glipizide, sitagliptin, melamine and cyanoguanidine, respectively. The proposed method was validated as per ICH criteria with respect to linearity, accuracy, precision, specificity and robustness. The validated method was successfully applied for determination of the studied drugs in Janumet® and Engilor® tablets; also, the results were statistically compared to those obtained by the reported spectrophotometric method and no significant difference was found between them. This method permitted the accurate simultaneous determination of the studied drugs, indicating its ability to be used for routine quality control assays of these drugs.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9318541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Four Kinds of Polymer Microspheres Prepared by the Seed Swelling Method Used to Purify the Industrial Production of Phytol. 用种子膨胀法制备的四种聚合物微球，用于净化工业生产的植物醇。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-07-10 DOI: 10.1093/chromsci/bmad006

Shixiang Liu, Zhipeng Fang, Yanan Li, Linlin Kang, Hailin Cong, Youqing Shen, Bing Yu

引用次数: 0

Chemical Profiling of Shen-Wu-Yi-Shen Tablets Using UPLC-Q-TOF-MS/MS and Its Quality Evaluation Based on UPLC-DAD Combined with Multivariate Statistical Analysis. 基于UPLC-DAD结合多元统计分析的UPLC-Q-TOF-MS/MS神武一味片化学成分分析及其质量评价

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-07-10 DOI: 10.1093/chromsci/bmae001

Yudan Mei, Yumei Hu, Xiaoqian Tao, Jing Shang, Mengyu Qian, Fengtai Suo, Jifeng Li, Liang Cao, Zhenzhong Wang, Wei Xiao

{"title":"Chemical Profiling of Shen-Wu-Yi-Shen Tablets Using UPLC-Q-TOF-MS/MS and Its Quality Evaluation Based on UPLC-DAD Combined with Multivariate Statistical Analysis.","authors":"Yudan Mei, Yumei Hu, Xiaoqian Tao, Jing Shang, Mengyu Qian, Fengtai Suo, Jifeng Li, Liang Cao, Zhenzhong Wang, Wei Xiao","doi":"10.1093/chromsci/bmae001","DOIUrl":"10.1093/chromsci/bmae001","url":null,"abstract":"Shen-Wu-Yi-Shen tablets (SWYST) is a traditional Chinese medicine prescription used for treating chronic kidney disease (CKD). This study aims to characterize the constituents in SWYST and evaluate the quality based on the quantification of multiple bioactive components. SWYST samples were analyzed with ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and a data-processing strategy. As a result, 215 compounds in SWYST were unambiguously identified or tentatively characterized, including 14 potential new compounds. Meanwhile, strategies based on characteristic fragments for rapid identification were summarized, indicating that the qualitative method is accurate and feasible. Notably, the glucose esters of laccaic acid D-type anthraquinone were first found and their fragmentation patterns were described by comparing that of O-glycoside isomers. Besides, based on comparisons of the cleavage ways of mono-acyl glucose with different acyl groups or acylation sites, differences in fragmentation pathways between 1,2-di-O-acyl glucose and 1,6-di-O-acyl glucose were proposed for the first time and verified by reference substances. In addition, a validated UPLC-DAD was established for the determination of 11 major bioactive components related to treatment of CKD (albiflorin, paeoniflorin, 2,3,5,4'-tetrahydroxy-stilbene-2-O-β-d-glucoside (TSG), 1-O-galloyl-2-O-cinnamoyl-β-d-glucose, emodin-8-O-β-d-glucoside, chrysophanol-O-β-d-glucoside, aloe-emodin, rhein, emodin, chrysophanol and physcion). Moreover, TSG and 1-O-galloyl-2-O-cinnamoyl-β-d-glucose were found as the quality markers related to the origins of SWYST based on multivariate statistical analysis. Conclusively, the findings in this work provide a feasible reference for further studies on quality research and mechanisms of action in treating CKD.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139512453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Relatively Simple Sample Pretreatment Strategies to Selectively Remove Chromatographic Interfering Peaks of Polysorbate 80 from Liquid Oral Finished Drug Product. 开发相对简单的样品预处理策略，从液体口服药物成品中选择性去除聚山梨醇酯 80 的色谱干扰峰。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-07-10 DOI: 10.1093/chromsci/bmad064

Sarju Adhikari, Shane N Berger, Abu M Rustum

{"title":"Development of Relatively Simple Sample Pretreatment Strategies to Selectively Remove Chromatographic Interfering Peaks of Polysorbate 80 from Liquid Oral Finished Drug Product.","authors":"Sarju Adhikari, Shane N Berger, Abu M Rustum","doi":"10.1093/chromsci/bmad064","DOIUrl":"10.1093/chromsci/bmad064","url":null,"abstract":"Polysorbate 80 (PS 80) is a nonionic surfactant, used in myriad of pharmaceutical, food and cosmetic formulations. PS 80 components have strong UV absorbance and retain under reversed-phase chromatographic conditions, significantly masking sections of the chromatogram. PS 80-related peaks interferences in a sample are common and can be difficult to separate from the analyte peaks. A liquid oral finished product (LOFP) containing PS 80 and Ivermectin as the active pharmaceutical ingredient (API) was selected for this study. Herein, we report two sample pretreatment strategies focusing on the selective removal of PS 80 from the LOFP. Both methods significantly reduce and/or practically eliminate excipients and PS 80-related peaks interferences from the LOFP without a negative impact on the API and its key-related substances recovery. The solid-phase extraction (SPE) strategy uses a C18 SPE followed by a silica gel SPE, whereas the liquid-liquid extraction strategy uses in situ-generated sodium caprylate for the removal of formulation excipients and PS 80. These methods can significantly increase the reliability of high-performance liquid chromatography methods and decrease false positive out-of-specifications events because of coelution of PS 80-related peaks with peaks of interest.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10018715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of 11 Kinds of Hair Dyes in Hair-Dyeing Products by Liquid Chromatography-Tandem Mass Spectrometry. 利用液相色谱-串联质谱法测定染发产品中的 11 种染发剂。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-07-10 DOI: 10.1093/chromsci/bmad071

Yu-Xiang Gu, Li-Song Chen, Lei Nie

引用次数: 0

Theoretical Study of Non-Isothermal Gradient Elution Liquid Chromatography. 非等温梯度洗脱液相色谱法的理论研究

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-07-10 DOI: 10.1093/chromsci/bmad076

Nazia Rehman, Ayesha Parveen, Shamsul Qamar

{"title":"Theoretical Study of Non-Isothermal Gradient Elution Liquid Chromatography.","authors":"Nazia Rehman, Ayesha Parveen, Shamsul Qamar","doi":"10.1093/chromsci/bmad076","DOIUrl":"10.1093/chromsci/bmad076","url":null,"abstract":"A two-component model of gradient elution chromatography is investigated to theoretically study the effects of simultaneous variations in temperature and solvent strength on the retention behaviors of elution profiles in thermally insulated liquid chromatographic columns. The gradient elution technique is based on the gradual increase or decrease in eluent strength during the chromatographic operation by varying the composition of the mobile phase. The enthalpy of adsorption is primarily responsible for internal temperature variations inside the column, as heat adsorbs during the adsorption process and releases in the desorption phase. Both types of variations change the propagation speeds of moving pulses inside the column which can lead to better separation of the components and a reduction in the recycling time for the next injection. The equilibrium dispersive model (EDM) coupled with the energy balance equation for temperature and transport equation for the volume fraction of the solvent is utilized to simulate this complex process. The resulting nonlinear model equations are approximated by applying a semi-discrete second-order finite volume scheme. The numerical solutions are used to study the impact of a gradient starting and ending times, volume-fraction of the solvent, solvent strength parameter, the slope of gradient, enthalpy of adsorption, injection temperature, and the ratio of specific heats on the concentration profiles.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10554334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0