Journal of chromatographic science最新文献_第2页

Quantification of Agnuside in Human Plasma with a Novel High-Performance Liquid Chromatographic Method and Pharmacokinetic Study. 新型高效液相色谱法定量测定人血浆中agn甙及药代动力学研究。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-01-14 DOI: 10.1093/chromsci/bmaf007

Derya Egeli, Gizem Tiris, Evrim Kepekci Tekkeli

引用次数: 0

Correction to: Isolation of Methoxyfuranocoumarins From Ammi majus by Centrifugal Partition Chromatography. 更正：用离心分离色谱法从 Ammi majus 中分离出甲氧基呋喃香豆素。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-01-14 DOI: 10.1093/chromsci/bmae045

引用次数: 0

Simultaneous Detection Method of 11 Respiratory Drug Substances Including Theobromine, Oxymetazoline, etc. in Adulterated Dietary Supplements Using Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry and Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry Analysis. 利用液相色谱-电喷雾离子化-串联质谱法和液相色谱-四极杆飞行时间质谱分析法同时检测掺假膳食补充剂中的可可碱、羟甲唑啉等 11 种呼吸道药物物质的方法

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-01-14 DOI: 10.1093/chromsci/bmae044

Mi Jin Kim, Hwan Seong Choi, Hyunil Shin, Ji Hyun Lee, Nam Sook Kim, Hyungil Kim

{"title":"Simultaneous Detection Method of 11 Respiratory Drug Substances Including Theobromine, Oxymetazoline, etc. in Adulterated Dietary Supplements Using Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry and Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry Analysis.","authors":"Mi Jin Kim, Hwan Seong Choi, Hyunil Shin, Ji Hyun Lee, Nam Sook Kim, Hyungil Kim","doi":"10.1093/chromsci/bmae044","DOIUrl":"10.1093/chromsci/bmae044","url":null,"abstract":"Recently, the demand for respiratory disease-related products has surged due to the influence of coronavirus disease 2019, prompting warnings about illegal dietary supplements containing unauthorized substances. Additionally, adulterated dietary supplements are continuously detected in open markets, posing significant public health safety problem. In this study, we developed and validated an analytical method for 11 respiratory drug substances using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) and proposed optimal conditions for LC-quadrupole time-of-flight MS (LC-QTOF-MS) to determine the fragmentation patterns of each substance. This method underwent thorough validation considering specificity, linearity, limits of detection and quantification, accuracy, precision, matrix effect, stability, etc. All results met international guidelines. These validated methods were applied to 52 dietary supplements advertised for treating respiratory diseases and enhancing respiratory function, among which one sample was found to contain 313.7 mg/g of theobromine. This determination was made by comparing the product ion ratios with the standards and subsequent quantification. To re-confirm the detected substances, their fragmentation patterns were compared with those of the standards using LC-QTOF-MS. In conclusion, the mass-based information, coupled with the LC-ESI-MS/MS method development, can be successfully applied to rapidly identify 11 respiratory drug substances in illegal dietary supplements used for respiratory disease treatment. The developed simultaneous detection method contributes to public health and safety improvements.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing the Active Components and Therapeutic Targets of Sanao Decoction against Chronic Obstructive Pulmonary Disease Using Quantitative Analysis of Multi-Components by Single Marker and Network Pharmacology. 单标记多组分定量分析及网络药理学揭示三脑汤治疗慢性阻塞性肺疾病的有效成分及治疗靶点。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-01-14 DOI: 10.1093/chromsci/bmaf002

Wanli Ji, Jiahong Wang, Yan Huo, Cheng Hu, Yifan Zhang

{"title":"Revealing the Active Components and Therapeutic Targets of Sanao Decoction against Chronic Obstructive Pulmonary Disease Using Quantitative Analysis of Multi-Components by Single Marker and Network Pharmacology.","authors":"Wanli Ji, Jiahong Wang, Yan Huo, Cheng Hu, Yifan Zhang","doi":"10.1093/chromsci/bmaf002","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf002","url":null,"abstract":"As a traditional Chinese medicine, Sanao decoction (SAD) has been used to treat chronic obstructive pulmonary disease (COPD) for multi-years. However, the potential mechanism and targets for its effects of SAD remain unknown. The 94 components of SAD were identified by UPLC-LTQ-Orbitrap MS. Meanwhile, the quantitative analysis of multi-components by single marker (QAMS) method was used to control the quality of SAD, including ephedrine hydrochloride, amygdalin, liquiritin, liquiritigenin, glycyrrhizic acid and glycyrrhetinic acid. The method was strictly validated with recovery (90.0-110.0%), precision [relative standard deviation (RSD), 0.79-2.01%], stability (RSD, 1.84-2.64%), repeatability (RSD, 0.45-3.03%) and relative correction factors (RSD, 0.28-2.67%), respectively. All the compounds showed good linearities (R2 > 0.999). Subsequently, 37 target proteins of SAD in the treatment of COPD were screened. The \"Compounds-Targets\" interaction and protein-protein interaction network found that TNF-α, IL-6 and VEGFA may act a crucial role in the treatment of COPD by SAD. Molecular docking demonstrated that TNF-α had good affinity with the main components of SAD. A strategy of QAMS and network pharmacology was a novel method to assess the quality control of SAD and uncover the targets and potential mechanism of SAD in the treatment of COPD.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Method Validation for Estimation of Imidacloprid and its Metabolites in Maize and Soil by LCMS-MS. 方法用液相色谱-质谱法测定玉米和土壤中吡虫啉及其代谢物的方法验证。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-01-14 DOI: 10.1093/chromsci/bmaf005

Sandeep Kaur, Smriti Sharma, Balpreet K Kang

{"title":"Method Validation for Estimation of Imidacloprid and its Metabolites in Maize and Soil by LCMS-MS.","authors":"Sandeep Kaur, Smriti Sharma, Balpreet K Kang","doi":"10.1093/chromsci/bmaf005","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf005","url":null,"abstract":"Validation of Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method was performed for estimation of imidacloprid (IM) and its metabolites in maize leaves, immature kernels, mature kernels, stalk, and soil using liquid chromatograph tandem mass spectrometry, coupled with electrospray ionization. The extraction in different matrices of maize and soil was performed using acetonitrile +0.1% formic acid followed by clean-up with primary secondary amine sorbent and anhydrous magnesium sulfate. The method was validated in terms of selectivity, linearity, limit of detection, limit of quantification, matrix effect, ion ratios, quality control, robustness, accuracy, and precision. The validation of all parameters was done in accordance with European Commission's Directorate-General for Health and Food Safety (DG SANTE) guidelines. A linear relationship with high correlation coefficients R2 > 0.99 was obtained for solvents and different matrices viz., maize leaves, immature kernels, mature kernels, stalk, and soil. The recovery and relative standard deviations were ˃78% and ˂5.4%, respectively. This method permits a simple, sensitive, accurate, cost-effective, precise, and rapid extraction of IM and its metabolites from maize leaves, immature kernels, mature kernels, stalks, and soil. This can help the residue analysts to address effective residue estimation, regular monitoring of residues and can also aid in the regulatory and food safety concerns about the usage of IM in maize.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Separation of Three Polar Compounds with Similar Polarities from Swertia mussotii by a Combination of Two Counter-Current Chromatography Modes. 两种逆流色谱法联合分离獐牙菜中极性相近的3种极性化合物。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-01-14 DOI: 10.1093/chromsci/bmaf006

Hongmei Li, Tao Chen, Cheng Shen, Shuo Wang, Juyuan Luo, Zhibo Song, Yumei Ma, Aijing Li, Weihang Lu, Hailun Feng, Yulin Li

{"title":"Separation of Three Polar Compounds with Similar Polarities from Swertia mussotii by a Combination of Two Counter-Current Chromatography Modes.","authors":"Hongmei Li, Tao Chen, Cheng Shen, Shuo Wang, Juyuan Luo, Zhibo Song, Yumei Ma, Aijing Li, Weihang Lu, Hailun Feng, Yulin Li","doi":"10.1093/chromsci/bmaf006","DOIUrl":"https://doi.org/10.1093/chromsci/bmaf006","url":null,"abstract":"Separation of polar compounds especially with similar polarities is challenging. In the present study, three polar compounds with similar polarities, including gentiopicroside, sweroside and mangiferin, have been successfully separated from Swertia mussotii by a combination of two counter-current chromatography modes. With the selected solvent system of ethyl acetate/n-butanol/water (8/2/10, v/v), a continuous injection mode was firstly employed. As a result, 10.4 g of mangiferin with purity higher than 98% and 8 g of Peak I mainly composed of gentiopicroside and sweroside could be easily obtained from 24 g of the sample by counter-current chromatography through eight injections. Then, a recycling counter-current chromatography mode was introduced for the separation of gentiopicroside and sweroside. Through six cycles, 5.3 g of gentiopicroside and 0.8 g of sweroside with purity higher than 98% were obtained from 8 g of Peak I. The research provides a reference for the large-scale separation of polar compounds with similar polarities.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"63 2","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Validation of a New High-Performance Liquid Chromatography-Ultraviolet Assay for Quantification of Mitoxantrone in Plasma of BALB/c-nu Mice. 开发并验证用于 BALB/c-nu 小鼠血浆中米托蒽醌定量的新型高效液相色谱-紫外检测法

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-01-09 DOI: 10.1093/chromsci/bmae007

Yanru Tao, Hua Zhao, Yujie Xiang, Jin Li, Yanting Li, Jiangling Hu, Hongmei Wang, Xinhui Jiang

{"title":"Development and Validation of a New High-Performance Liquid Chromatography-Ultraviolet Assay for Quantification of Mitoxantrone in Plasma of BALB/c-nu Mice.","authors":"Yanru Tao, Hua Zhao, Yujie Xiang, Jin Li, Yanting Li, Jiangling Hu, Hongmei Wang, Xinhui Jiang","doi":"10.1093/chromsci/bmae007","DOIUrl":"10.1093/chromsci/bmae007","url":null,"abstract":"The concentration of mitoxantrone in the blood of mice was determined by a high-performance liquid chromatography-ultraviolet method with aloe-emodin as the internal standard. The separation was performed on a Hypersil BDS2 column (4.6 × 250 mm, 5 μm) as the analytical column, the mobile Phase A was acetonitrile, and B was 20-mM potassium dihydrogen phosphate (adding 1% triethylamine and adjusting the pH to 2.8 with phosphoric acid) and 4.6-mM sodium octyl sulfonate. The flow rate was 1.0 mL·min-1, the detection wavelength was 243 nm, the column temperature is 25 ± 5°C and the injection amount was 20 μL. Finally, the linear range of mitoxantrone was 5-200 μg·mL-1, and the correlation coefficient was r = 0.9999. The recovery rate of the method was 91.93-105.5%, and the extraction recovery rate was 91.45-105.5%. The intraday precision and interday precision were <3.29% (limit of detection = 0.3 μg·mL-1). The HPLC method established in this paper was simple, rapid, sensitive and accurate, and can be used to determine the content of mitoxantrone in mouse plasma after tail vein injection.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140140323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability-Indicating Liquid Chromatographic Method Development for the Simultaneous Determination of Amitriptyline Hydrochloride and Propranolol Hydrochloride in Tablet Dosage Form. 稳定性指示液相色谱法同时测定片剂中盐酸阿米替林和盐酸心得洛尔的含量。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-01-09 DOI: 10.1093/chromsci/bmae060

Obi Reddy Chabala, Simon Haque Md, Durai Ananda Kumar Thirumoorthy

{"title":"Stability-Indicating Liquid Chromatographic Method Development for the Simultaneous Determination of Amitriptyline Hydrochloride and Propranolol Hydrochloride in Tablet Dosage Form.","authors":"Obi Reddy Chabala, Simon Haque Md, Durai Ananda Kumar Thirumoorthy","doi":"10.1093/chromsci/bmae060","DOIUrl":"10.1093/chromsci/bmae060","url":null,"abstract":"The combination of the tricyclic antidepressant amitriptyline hydrochloride (AMH) and the non-selective beta-adrenergic blocker propranolol hydrochloride (PPH) is used for migraine prophylaxis. Higher doses of AMH trigger cardiac arrhythmias, anxiety, tachycardia, convulsions, hyperglycemia and anticholinergic side effects. The combined dosage formulation of AMH and PPH leads to drug-drug interactions; causes sedation, xerostomia, dysuria, insomnia and bradycardia; and results in patient non-compliance. The quantification of AMH and PPN becomes essential, especially for combination formulations, in addition to regular quality control to avoid clinical issues. Considering these facts into account, the reverse-phase -high-performance liquid chromatography (RP-HPLC) method was developed in accordance with International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use Q2(R1) guidelines for the simultaneous determination of AMH and PPH. The HPLC separation was performed on an HPLC system (Shimadzu, Japan, Prominence I series 2030C) using a Shimadzu Shim-Pack GIST C18 column (100 mm × 4.6 mm, 5 μ), which was equipped with an ultraviolet detector at the isosbestic point 238 nm. The mixture of acetonitrile and orthophosphoric acid (pH 3.5) in a ratio of 35:65 v/v with a flow rate of 0.75 mL/min was used as the mobile phase. The regression coefficients of AMH (r2 > 0.998) and PPH (r2 > 0.999) show good linearity between peak areas and drug concentration ranges. The limits of detection (AMH = 0.67 μg/mL, PPH = 0.67 μg/mL) and limits of quantification (AMH = 2.04 μg/mL, PPH = 2.05 μg/mL) demonstrated the higher detection sensitivity of the proposed method.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioanalytical Method Development and Validation of Doxorubicin and Lapatinib in Rat Plasma Using UHPLC-HESI-LTQ-MS. 利用 UHPLC-HESI-LTQ-MS 对大鼠血浆中多柔比星和拉帕替尼的生物分析方法进行开发和验证

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-01-09 DOI: 10.1093/chromsci/bmad090

Shaik Khaja Moinuddin, Pirangi Srikanth, Parul Sharma, Sukhendu Nandi

{"title":"Bioanalytical Method Development and Validation of Doxorubicin and Lapatinib in Rat Plasma Using UHPLC-HESI-LTQ-MS.","authors":"Shaik Khaja Moinuddin, Pirangi Srikanth, Parul Sharma, Sukhendu Nandi","doi":"10.1093/chromsci/bmad090","DOIUrl":"10.1093/chromsci/bmad090","url":null,"abstract":"Cancer is considered a silent killer. The complexity of cancer makes it earn that title. So far there are only a few approaches to treat cancer. Among them, chemotherapy is considered the best approach. Many chemotherapeutical compounds are commercially available in the market. Among them, doxorubicin (DOX) and lapatinib (LAP) are considered blockbuster molecules. However, DOX suffers from poor bioavailability and exhibits cardiotoxicity. Interestingly, a fixed dose combination of DOX and LAP significantly decreases the cardiotoxic effect of DOX. To enhance the oral bioavailability of DOX and to avail the synergistic effect of LAP, many formulations have been made. To quantify both compounds in any formulation or biological matrix, an Liquid chromatography-Mass Spectrometry (LC-MS) method is required. In this present study, a simple and rapid Ultra High-Performance Liquid Chromatography - Heated Electron Spray Ionization - Mass Spectrometry (UHPLC-HESI-MS) bioanalytical method was developed. The developed method was validated as per the regulatory guidelines. The validated bioanalytical method had a lower limit of quantification of 0.75 ng. A simple protein precipitation technique was optimized to extract the compounds from the rat plasma. All the validation parameters were found to be within the limits as per the regulatory guidelines. A novel and rapid analytical method was successfully developed and validated. This developed method can be used to quantify the DOX and LAP in any formulation and biological matrix.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138804829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Elucidation of Novel Degradation Impurities of Ibrutinib in Ibrutinib Tablets Using Preparative Chromatography, LCMS, HRMS and 2D NMR Techniques. 利用制备色谱、LCMS、HRMS 和 2D NMR 技术阐明伊布替尼片中伊布替尼新型降解杂质的结构。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2025-01-09 DOI: 10.1093/chromsci/bmae002

Rajender Reddy Yerla, Surendrababu Manubolusurya, Saravanakumar Meganathan, Veerababu Madalapu, Gopal Vaidyanathan

{"title":"Structural Elucidation of Novel Degradation Impurities of Ibrutinib in Ibrutinib Tablets Using Preparative Chromatography, LCMS, HRMS and 2D NMR Techniques.","authors":"Rajender Reddy Yerla, Surendrababu Manubolusurya, Saravanakumar Meganathan, Veerababu Madalapu, Gopal Vaidyanathan","doi":"10.1093/chromsci/bmae002","DOIUrl":"10.1093/chromsci/bmae002","url":null,"abstract":"Ibrutinib is an orally administered compound that functions as an irreversible covalent inhibitor of the Bruton tyrosine kinase, an essential element in multiple cellular processes including B-cell differentiation, proliferation, migration, survival and apoptosis. The compound has been found to demonstrate efficacy against a range of B-cell malignancies. The drug product is available in oral tablet and capsule formulations. The drug degradation profiles of tablets dosage form were assessed in accordance with regulatory guidelines. The results indicate that the drug substance is susceptible to alkaline and oxidative stress. The oxidation degradation led to the identification of three significant unknown degradation impurities. The three compounds were isolated through the application of preparative liquid chromatography, and their structures were determined using analytical techniques such as liquid chromatography-mass spectrometry, high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy. Utilizing structural elucidation data, predictions were made regarding the composition of impurities, revealing them to be novel degradation impurities that bear structural resemblance to ibrutinib. Additionally, potential pathways for the formation of the impurities were proposed.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139712296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0