An High Performance Thin Layer Chromatography (HPTLC) Validated Method for the Determination of Ephedrine Alkaloids in Multi-Plant Formulations.

IF 1.5 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of chromatographic science Pub Date : 2025-03-27 DOI:10.1093/chromsci/bmaf020

Simonetta Gemma, Giuseppina Multari, Francesca R Gallo

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引用次数: 0

Abstract

Ephedrine is one component of a popular traditional Chinese multi-plant formulation used to treat asthma, fever, coughs and lately COVID-19. The aim of this study is to develop and validate a simple and rapid quali-quantitative High Performance Thin Layer Chromatography (HPTLC) densitometry method to detect ephedrine, the most abundant alkaloid of Ephedra plant, and to analyze free sale ephedra-based capsules. This quick, simple and low requirement method is cost-effective and allows screening up to 19 samples all together in a single layer. During the development of this method, an extraction solvent mixture composed of methanol/water (2:1, v/v) and a mobile phase constituted of ammonia/methanol/dichloromethane (1:10:40, v/v/v) gave the best results. The identity and purity of ephedrine peak were confirmed by matching Rf value and UV-spectrum with its reference standard. Lastly, samples were quantitated by densitometry at 500 nm after ninhydrin derivatization. Linearity was obtained in the concentration range of 0.062-0.146 μg/band. Limit of detection and limit of quantification (LOQ) were found to be 0.0020 μg/band and 0.0067 μg/band, respectively. This technique is useful to screen multiple samples at the same time with a limited use of solvents and reagents so that it can be considered a fast and simple procedure.

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高效薄层色谱法测定多种植物制剂中麻黄碱类生物碱含量。

麻黄碱是一种流行的中国传统多植物配方的一种成分，用于治疗哮喘、发烧、咳嗽和最近的COVID-19。本研究旨在建立一种快速、高效液相色谱（HPTLC）定量检测麻黄植物中含量最高的生物碱麻黄碱的方法，并对麻黄基胶囊进行定量分析。这种快速，简单和低要求的方法具有成本效益，并允许在单层中筛选多达19个样品。以甲醇/水（2:1,v/v）为萃取溶剂，以氨/甲醇/二氯甲烷（1:10:40,v/v/v）为流动相，萃取效果最佳。通过与参比标准品的Rf值和紫外光谱比对，确定了麻黄碱峰的身份和纯度。最后，在茚三酮衍生化后，在500 nm处进行密度测定。在0.062 ~ 0.146 μg/波段范围内呈线性关系。检出限为0.0020 μg/波段，定量限为0.0067 μg/波段。该技术适用于在有限的溶剂和试剂使用情况下同时筛选多个样品，因此它可以被认为是一个快速和简单的程序。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.