Simultaneous Determination of 30 Flavonoids in Jinqiancao Granules by LC-MS/MS.

IF 1.5 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of chromatographic science Pub Date : 2025-05-07 DOI:10.1093/chromsci/bmaf028

Jiajie Wang, Yimeng Liu, Shuang Xie, Luyao Wang, Beibei Yao, Xu Zhang, Wanwan Zhang, Chunying Wang

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引用次数: 0

Abstract

In order to determine the content of flavonoid components in Jinqiancao granules efficiently and accurately, a high-efficiency quantitative analysis method utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed. As per the guidelines from the International Council for Harmonization, a comprehensive method validation was conducted for this method. Experimental data demonstrated that the method exhibited excellent accuracy, specificity and reproducibility in the multiple reaction monitoring mode, facilitating the swift and accurate quantification of flavonoid content. Through chemometrics analysis, significant differences in the content of flavonoid compounds were observed among different batches of Jinqiancao granules samples. Isoschaftoside, Vicenin-2, Schaftoside and myricitrin can be used as the main quality markers for Jinqiancao granules. The research results provided a scientific basis for the quality control of Jinqiancao granules, which helps enhance the consistency and reliability of the product, thereby ensuring the superior quality of the preparation.

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LC-MS/MS同时测定金草颗粒中30种黄酮类化合物的含量。

为了高效、准确地测定金前草颗粒中黄酮类成分的含量，建立了液相色谱-串联质谱（LC-MS/MS）高效定量分析方法。根据国际协调理事会的指导方针，对该方法进行了全面的方法验证。实验数据表明，该方法在多反应监测模式下具有良好的准确性、专一性和重复性，有利于快速、准确地定量黄酮类化合物含量。通过化学计量学分析，发现不同批次金前草颗粒样品中黄酮类化合物含量存在显著差异。异Schaftoside、Vicenin-2、Schaftoside和myricitrin可作为金千草颗粒的主要质量标志。研究结果为金前草颗粒的质量控制提供了科学依据，有助于提高产品的一致性和可靠性，从而保证制剂的优良品质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.