Comprehensive Profiling of the Chemical Constituents in Dioscorea Bulbifera Using High Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry.
Qing Zhang, Jiasi Huang, Na Hu, You Peng, Zhiqiang Lei, Yun Ling
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Abstract
The rhizome of Dioscorea bulbifera (RDB) has been used in folk and traditional Chinese medicine for the treatment of thyroid diseases and cancer. However, the chemical constituents are not fully clear. In this study, high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry was successfully used for the comprehensive characterization of chemical constituents in RDB. As a result, a total of 40 compounds were identified in RDB by comparison with the authentic standards or reported references, including 13 flavonoids, 2 diterpenoids, 2 stilbenoids, 12 steroidal saponins, and 11 phenolic derivatives. This study comprehensively elucidated the chemical basis of RDB and provided a robust foundation for the quality control and pharmacology research in the future.
期刊介绍：
The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.
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