Journal of chromatographic science最新文献_第7页

Correction to: AGREE and ESA for Greenness Assessment of a Novel Validated RP-HPLC Method for Simultaneous Determination of Aspirin, Warfarin and Clopidogrel in Rat Plasma: Application to Pharmacokinetic Study of the Possible Interaction between the Three Drugs. 更正：AGREE和ESA用于同时测定大鼠血浆中阿司匹林、华法林和氯吡格雷的新型经验证的RP-HPLC方法的绿色评估：在三种药物之间可能相互作用的药代动力学研究中的应用。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-10-01 DOI: 10.1093/chromsci/bmad085

引用次数: 0

Development and Validation of UPLC Tandem Mass Spectrometry Assay for Ceftibuten and Sulbactam in Human Plasma. 人血浆中头孢布坦和舒巴坦的 UPLC 串联质谱分析法的开发与验证

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-10-01 DOI: 10.1093/chromsci/bmae010

Karla Paulina Valero-Rivera, Martin Magaña-Aquino, María Del Carmen Romero-Méndez, Miriam Nayeli Morales-Barragán, Julia Sagahón-Azúa, Susanna Edith Medellín-Garibay, Rosa Del Carmen Milán-Segovia, Fidel Martínez-Gutiérrez, Silvia Romano-Moreno

{"title":"Development and Validation of UPLC Tandem Mass Spectrometry Assay for Ceftibuten and Sulbactam in Human Plasma.","authors":"Karla Paulina Valero-Rivera, Martin Magaña-Aquino, María Del Carmen Romero-Méndez, Miriam Nayeli Morales-Barragán, Julia Sagahón-Azúa, Susanna Edith Medellín-Garibay, Rosa Del Carmen Milán-Segovia, Fidel Martínez-Gutiérrez, Silvia Romano-Moreno","doi":"10.1093/chromsci/bmae010","DOIUrl":"10.1093/chromsci/bmae010","url":null,"abstract":"A sensitive and rapid ultra-performance liquid chromatography coupled with -tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to determine ceftibuten (CTB) and sulbactam (SUL) in human plasma. An ACQUITY UPLC HSS T3 C18 (2.1 × 100 mm), 1.8 μm column with gradient elution of water (0.1% formic acid) and acetonitrile was used for separation at a flow rate of 0.2 mL/min. This method involves a simple sample preparation with acetonitrile. The calibration curves of CTB and SUL in plasma showed good linearity over the concentration range of 0.50-25 μg/mL and with a correlation coefficient (r2) >0.99. This method was validated in terms of selectivity, linearity, precision, accuracy and stability. High precision was obtained with coefficients of variation <15%. Excellent recovery in the range of 90-104% was achieved for CTB and SUL was 86-110%. The method has the potential utility to support pharmacometric modeling in clinical practice and biopharmaceutic studies.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"783-788"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability-Indicating HPLC Method for Determination of Ibuprofen and Famotidine Degradation Products. 测定布洛芬和法莫替丁降解产物的稳定性指示高效液相色谱法。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-10-01 DOI: 10.1093/chromsci/bmae027

Nurdan Atilgan, Gizem Tabansiz, Ezgi Turkes, Nagehan Sarracoglu, Asuman Aybey Doganay, Onur Pinarbasli

{"title":"Stability-Indicating HPLC Method for Determination of Ibuprofen and Famotidine Degradation Products.","authors":"Nurdan Atilgan, Gizem Tabansiz, Ezgi Turkes, Nagehan Sarracoglu, Asuman Aybey Doganay, Onur Pinarbasli","doi":"10.1093/chromsci/bmae027","DOIUrl":"10.1093/chromsci/bmae027","url":null,"abstract":"A new stability-indicating high-performance liquid chromatographic method for the quantitative determination of ibuprofen and famotidine degradation products in combined pharmaceutical products was developed and validated. The current aim of this study is to develop a rapid, accurate and robust analytical stability indicating impurity method that can separate ibuprofen, famotidine and their related impurities by using a reversed-phase high-performance liquid chromatography. A Zorbax SB-Phenyl column (4.6 × 150 mm2, 5-μm particle size) with mobile phase containing phosphate buffer solution with a pH value of 3.0 and acetonitrile was used. The flow rate was 0.8 mL/min and the analytes were detected by UV detector at 265 nm. The retention times of ibuprofen and famotidine were 18.43 and 5.14 min, respectively. This method was validated to confirm specificity, linearity, sensitivity (limit of detection and limit of quantitation), precision, accuracy, robustness and sample stability according to the International Conference on Harmonization guidelines. Studies have been completed and reported with two active substances in the combined dosage form and seven impurities in total. There is no method in the literature that simultaneously distinguishes and quantitatively analyzes both active substances and degradation products.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"751-760"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast and Easy Simultaneous Determination of Riboflavin, Folic Acid, All-Trans-Retinol and α-Tocopherol in Human Serum by LC/MS/MS for Bariatric Patients. 利用 LC/MS/MS 快速简便地同时测定减肥患者血清中的核黄素、叶酸、全反式-视黄醇和 α-生育酚。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-10-01 DOI: 10.1093/chromsci/bmae035

Romana Urinovska, Pavel Sistik, Marek Buzga, David Stejskal

{"title":"Fast and Easy Simultaneous Determination of Riboflavin, Folic Acid, All-Trans-Retinol and α-Tocopherol in Human Serum by LC/MS/MS for Bariatric Patients.","authors":"Romana Urinovska, Pavel Sistik, Marek Buzga, David Stejskal","doi":"10.1093/chromsci/bmae035","DOIUrl":"10.1093/chromsci/bmae035","url":null,"abstract":"The aim of this study was to develop and validate methods for the determination of vitamins B2, B9, E and A in serum using liquid chromatography with mass spectrometry (MS) detection. Vitamin analysis was performed using an ultra performance liquid chromatography combined with tandem MS. The compounds were separated on a BEH C18 RP column (2.1 × 100 mm, 1.7 μm) using a gradient elution with an analysis time of 10 min. Sample preparation included protein precipitation with ethanol. The concentration range in human serum was as follows: riboflavin 5-1000 nmol/L, folic acid 2.5-250 nmol/L, α-tocopherol 0.5-100 μmol/L and all-trans-retinol 25-2500 nmol/L. Accuracy and precision were validated according to Food and Drug Administration guidelines, with coefficients of variation ranging from 3.1-11.7% and recoveries from 94.4-107.5%. Routine monitoring of the complex range of vitamins in bariatric medicine is still not common. This is despite the fact that patients are at risk for glitch deficits, especially of a neurological nature. An analytical method that allows for the complex measurement of both water-soluble and fat-soluble vitamins is important and necessary for the clinical monitoring of bariatric patients. The method we have described could benefit both clinical practice and nutritional research.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"767-775"},"PeriodicalIF":1.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of Chiral Stationary Phase Based on 1,1'-bi-2-Naphthol for Chiral Enantiomer Separation. 基于 1,1'-bi-2-萘酚的手性固定相的制备，用于手性对映体分离。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-09-27 DOI: 10.1093/chromsci/bmae051

Kun Fan, Rui Dong, Wenqing Hou, Canyu Yang, Kongchun Sun, LvJing Xu, Bingquan Chang, Desheng Wang, Congcong Zhang, Baochun Shen

引用次数: 0

Novel Green Chromatographic Approaches for Estimation of a Triple Common Cold Pharmaceutical Combination. 估算常见感冒药三联复方的新型绿色色谱法

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-09-26 DOI: 10.1093/chromsci/bmae050

Rabab M Soliman, Yasmin Rostom, Yasmin M Fayez, Nadia M Mostafa, Hany H Monir

{"title":"Novel Green Chromatographic Approaches for Estimation of a Triple Common Cold Pharmaceutical Combination.","authors":"Rabab M Soliman, Yasmin Rostom, Yasmin M Fayez, Nadia M Mostafa, Hany H Monir","doi":"10.1093/chromsci/bmae050","DOIUrl":"https://doi.org/10.1093/chromsci/bmae050","url":null,"abstract":"Nowadays, there is a strong interest in the scientific community in developing innovative methodologies within a green analytical chemistry framework. Herein, we introduce the first chromatographic approaches for the concurrent estimation of paracetamol (PAR), carbinoxamine (CRX), and pseudoephedrine (PSE) intended to relieve COVID-19 and common cold symptoms. The first method was thin layer chromatography (TLC) densitometry, which depends on the separation of the studied medications on TLC silica gel plates using ethyl acetate: methanol: ammonia (7.0: 3.0: 0.2, by volume) as the developing system, and were scanned at 208.0 nm. The data were linear in the ranges of 1-25 μg/band for PAR, 1-25 μg/band for PSE and 0.1-5 μg/band for CRX. The second method was reversed-phase high-performance liquid chromatography separation on a Kromasil C18 column using a mixture of 0.01 M phosphate buffer containing 0.1% triethylamine (pH 3.5) adjusted with orthophosphoric acid and ethanol at a flow rate of 1.0 mL/min in a gradient program. The separated peaks were detected at 215.0 nm over a concentration range of 10-250 μg/mL for PAR, 5-35 μg/mL for PSE, and 0.5-25 μg/mL for CRX. Both approaches were validated according to International Conference on Harmonization (ICH) guidelines. Finally, the impact of these methods on the environment was evaluated by many tools.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A GC–MS Method for Determination of β-Propiolactone Residues in Inactivated Covid-19 Vaccines 测定灭活 Covid-19 疫苗中 β-丙内酯残留量的气相色谱-质谱方法

IF 1.3 4区化学

Journal of chromatographic science Pub Date : 2024-09-17 DOI: 10.1093/chromsci/bmae049

Seyedeh Zohreh Mirjalili, Zahra Tamiji, Mehrnoosh Shirangi, Mohsen Amini

{"title":"A GC–MS Method for Determination of β-Propiolactone Residues in Inactivated Covid-19 Vaccines","authors":"Seyedeh Zohreh Mirjalili, Zahra Tamiji, Mehrnoosh Shirangi, Mohsen Amini","doi":"10.1093/chromsci/bmae049","DOIUrl":"https://doi.org/10.1093/chromsci/bmae049","url":null,"abstract":"β-propiolactone is a common inactivator agent used in vaccines. Due to β-propiolactone carcinogenicity, complete hydrolysis of it is necessary to prevent cytotoxicity in mammalian cells. As a result, more attention should be paid to it at the clinic, and it is important to measure its trace amounts. β-propiolactone analysis is challenging due to its instability. A simple and fast gas chromatography–mass spectrometry method was developed for quantitation of residual β-propiolactone in inactivated coronavirus disease 2019 (Covid-19) vaccines. Caprolactone was used as an internal standard in sample solutions; the analysis was performed after extraction of analyte from vaccine media by ethyl acetate. The validity of the method was studied with a linearity of r2 &gt; 0.99 over the concentration range of 0.2–20 μg/mL with the limit of detection and the limit of quantification of 0.07 and 0.20 μg/mL, respectively. The target analyte β-propiolactone was not detected in the samples, demonstrating the test samples were qualified. The established method can be used for quality control of inactivated Covid-19 vaccines.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":"770 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142264533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ayurveda Detoxification Process Reduces Plumbagin from the Roots of Plumbago zeylanica L. - A RP-UFLC Analysis. 阿育吠陀排毒过程减少了 Plumbago zeylanica L. 根中的 Plumbagin - RP-UFLC 分析。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-09-06 DOI: 10.1093/chromsci/bmae048

Gireesh M Ankad, Harsha Hegde, Iranna B Kotturshetti

{"title":"Ayurveda Detoxification Process Reduces Plumbagin from the Roots of Plumbago zeylanica L. - A RP-UFLC Analysis.","authors":"Gireesh M Ankad, Harsha Hegde, Iranna B Kotturshetti","doi":"10.1093/chromsci/bmae048","DOIUrl":"https://doi.org/10.1093/chromsci/bmae048","url":null,"abstract":"Ayurveda describes purification process of certain herbal drugs to reduce the toxicity and make them suitable for therapeutic purpose. The objective of the study was to evaluate the effect of detoxification process on plumbagin (PG) from the Plumbago zeylanica L. roots in marketed samples. It involved procurement of market samples from five states of India viz. Andhra Pradesh, Gujarat, Maharashtra, Madhya Pradesh and Punjab. The roots were purified in lime water (LW) as mentioned in Ayurveda. Reverse Phase Ultra-Flow Liquid Chromatography method was validated for identification of PG in unprocessed and processed roots and in the media (LW) used for purification after processing. The data was statistically analyzed by Analysis of Variance (ANOVA) and tested for significance by the Dunnett multiple comparison test. Results were expressed as mean ± SD mg/g dry weight of extract. The study indicated that the PG was reduced quantitatively after processing, while the amount of PG found in the LW was observed to be increased, indicating the leaching of PG during the purification process. In conclusion, the detoxification process eliminates PG from its roots and discloses the leaching effect in the media for the first time.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green and Sustainable Analytical Chemistry-Driven Chromatographic Method Development for Stability Study of Apixaban Using Box-Behnken Design and Principal Component Analysis. 利用盒-贝肯设计和主成分分析法，为阿哌沙班的稳定性研究开发绿色和可持续的分析化学驱动色谱方法

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-08-20 DOI: 10.1093/chromsci/bmad033

Pintu Prajapati, Pooja Rajpurohit, Veera Shakar Pulusu, Shailesh Shah

{"title":"Green and Sustainable Analytical Chemistry-Driven Chromatographic Method Development for Stability Study of Apixaban Using Box-Behnken Design and Principal Component Analysis.","authors":"Pintu Prajapati, Pooja Rajpurohit, Veera Shakar Pulusu, Shailesh Shah","doi":"10.1093/chromsci/bmad033","DOIUrl":"10.1093/chromsci/bmad033","url":null,"abstract":"Apixaban (APX) is a novel anti-coagulant drug approved by USFDA. According to referred literature, numerous chromatographic methods such as RP-HPLC and high-performance thin-layer chromatography have been published for the stability study of APX. But these chromatographic methods have been developed using toxic organic solvents that are hazardous to the environment and unsafe for analysts. Hence, green and sustainable analytical chemistry-driven chromatographic method has been developed for the stability study of APX using safe organic solvents for the safety of analysts and the protection of the environment. APX was subjected to forced degradation for the development of a stability-indicating assay method. The method development was carried out by the implementation of chemometric and DoE approaches for minimizing solvent wastage. Principal component analysis was applied for the identification of critical method risk variables (MRVs) and method performance attributes. DoE-based response surface modelling was applied for the optimisation of critical MRVs. The greenness profile scales of published and developed chromatographic methods have been assessed by NEMI and AGREE methods for the estimation of APX. The developed method was found to be more eco-friendly and robust than the published chromatographic methods for the estimation of APX.","PeriodicalId":15430,"journal":{"name":"Journal of chromatographic science","volume":" ","pages":"649-659"},"PeriodicalIF":1.5,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9426873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Determination of General Active Ingredients in Topical Formulations by Gas Chromatography with Flame Ionization Detection (GC-FID). 气相色谱-火焰离子化检测器(GC-FID)同时测定外用制剂中一般有效成分。

IF 1.5 4区化学

Journal of chromatographic science Pub Date : 2024-08-20 DOI: 10.1093/chromsci/bmad086

Anna Ioutsi, Anastasia Sarnitskaya, Mikhail Sumtsov

引用次数: 0