UPLC-MS/MS Combined with Microdialysis for Determination of Two Active Components of Chuanxiong Rhizoma Extracts in Rat Brain: Application in Pharmacokinetic Study.

IF 1.5 4区 化学 Q4 BIOCHEMICAL RESEARCH METHODS
Yu Wang, Liang Jin, Xinhua Liu, Bing Shu, Jia Xu, Lingyun Pan
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引用次数: 0

Abstract

Chuanxiong Rhizoma (Chuanxiong), a traditional Chinese medicine, has been widely used to treat various nervous and cardiovascular system-related conditions. Its active components, senkyunolide A (SA) and 3-n-butylphthalide (NBP), have been proven effective in treating nervous system diseases. A new method was established based on microdialysis coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to estimate the concentrations of these components in brain extracellular fluid. Chromatographic separation was achieved using an Acquity UPLC BEH C8 column (2.1 × 100 mm, 1.7 μm) with acetonitrile and 0.1% formic acid as mobile phase. The calibration curves of SA and NBP were linear in the concentration ranges of 0.25-100.00 and 0.12-48.00 ng/mL, respectively, with a correlation coefficient above 0.9992. All validation parameters, including intra- and inter-day precision, accuracy, matrix effect and stability, were within the acceptance limits of bioanalytical guidelines. The validated method was successfully applied to study the pharmacokinetics of SA and NBP in rat brain microdialysis after oral administration of Chuanxiong extracts. The results showed that both components penetrated the brain and reached maximum concentrations in the microdialysates of 72.31 and 9.93 ng/mL at 1.50 and 1.58 h, respectively.

超高效液相色谱-质谱联用微透析法测定川芎提取物中两种有效成分在大鼠脑中的药动学研究
川芎是一种中药,被广泛用于治疗各种神经和心血管系统相关疾病。它的有效成分,仙丘内酯A (SA)和3-正丁基酞(NBP),已被证明对治疗神经系统疾病有效。建立了微透析联用超高效液相色谱-串联质谱(UPLC-MS/MS)测定脑外液中这些成分浓度的新方法。色谱分离采用Acquity UPLC BEH C8色谱柱(2.1 × 100 mm, 1.7 μm),乙腈和0.1%甲酸为流动相。SA和NBP分别在0.25 ~ 100.00和0.12 ~ 48.00 ng/mL范围内呈良好的线性关系,相关系数均在0.9992以上。所有验证参数,包括日内、日间精密度、准确度、基质效应和稳定性,均在生物分析指南的可接受范围内。将验证的方法应用于口服川芎提取物大鼠脑微透析中SA和NBP的药动学研究。结果表明,这两种成分均能穿透大脑,在1.50和1.58 h时,微透析液中的浓度分别达到72.31和9.93 ng/mL。
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来源期刊
CiteScore
2.90
自引率
7.70%
发文量
94
审稿时长
5.6 months
期刊介绍: The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.
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