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Journal of cyclic nucleotide and protein phosphorylation research最新文献

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Cyclic AMP phosphodiesterase activities in growing cells of baker's yeast (Saccharomyces cerevisiae). 酵母(Saccharomyces cerevisiae)生长细胞中的环AMP磷酸二酯酶活性。
Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01
K Suoranta
{"title":"Cyclic AMP phosphodiesterase activities in growing cells of baker's yeast (Saccharomyces cerevisiae).","authors":"K Suoranta","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The activities of two cyclic AMP phosphodiesterases of baker's yeast (Saccharomyces cerevisiae) were measured during diauxic batch growth on 2% glucose. The specific activity (units/mg of yeast protein) of the Mg-independent, high Km phosphodiesterase increased 20-fold throughout the 108 h cultivation. The specific activity of the Mg-dependent, low Km phosphodiesterase about doubled during glucose utilization and fell back to the initial level as the cells entered stationary phase.</p>","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14118353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The discovery of calmodulin--an historical perspective. Dedicated to the memory of Shiro Kakiuchi (1929-1984), a quiet leader in calcium research. 钙调素的发现——一个历史的视角。谨以此纪念钙研究领域一位默默无闻的领军人物——角内四郎(1929-1984)。
Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01
M M Appleman, T W Rall, J R Dedman
{"title":"The discovery of calmodulin--an historical perspective. Dedicated to the memory of Shiro Kakiuchi (1929-1984), a quiet leader in calcium research.","authors":"M M Appleman,&nbsp;T W Rall,&nbsp;J R Dedman","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14132475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stimulation of coronary guanylate cyclase by nicorandil (SG-75) as a mechanism of its vasodilating action. 尼可地尔(SG-75)刺激冠状动脉鸟苷酸环化酶作为其血管舒张作用的机制。
Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01
K Schmidt, R Reich, W R Kukovetz
{"title":"Stimulation of coronary guanylate cyclase by nicorandil (SG-75) as a mechanism of its vasodilating action.","authors":"K Schmidt,&nbsp;R Reich,&nbsp;W R Kukovetz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Since there is evidence suggesting that nicorandil (SG-75) relaxes coronary arterial smooth muscle by increasing cGMP levels, the effects of this vasodilator on soluble guanylate cyclase from bovine coronary arteries were studied more closely. It was found that nicorandil stimulated guanylate cyclase dose-dependently (3-30 mM) up to 100-fold the control value. Similar to nitroglycerin but in contrast to sodium nitroprusside, cysteine (0.5-20 mM) was required to obtain this stimulation. All other investigated thiols, except thiosalicylic acid which was partially able to mimic the cysteine effect, were ineffective. As evident from time course studies, nicorandil induced stimulation of guanylate cyclase was characterized by a lag-phase which could be avoided by preincubating the enzyme with nicorandil. The stimulatory effect of nicorandil was diminished in the presence of methylene blue, ferricyanide or hydroquinone. These results give further evidence that a) nicorandil exerts its vasodilating effect via stimulation of guanylate cyclase and b) nitrate esters, such as nitroglycerin or nicorandil, stimulate the enzyme, at least in vitro, only in the presence of cysteine or, to a lesser extent, thiosalicylic acid.</p>","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13995924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of alloxan on the islets of Langerhans: stimulation and inhibition of cyclic AMP production. 四氧嘧啶对朗格汉斯胰岛的影响:刺激和抑制环AMP的产生。
Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01
L A Borg, A J Bone
{"title":"Effects of alloxan on the islets of Langerhans: stimulation and inhibition of cyclic AMP production.","authors":"L A Borg,&nbsp;A J Bone","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Alloxan exerts a selective impairment of the insulin-producing B-cells of the islets of Langerhans, which may result in diabetes mellitus. The effects of alloxan on cyclic AMP metabolism in isolated mouse islets of Langerhans were investigated. Alloxan caused an immediate increase in islet content of cyclic AMP, whereas a subsequent glucose-stimulated increase of islet cyclic AMP content was inhibited in alloxan-exposed islets. No corresponding effects of the drug were, however, found on either islet adenylate cyclase or cyclic AMP phosphodiesterase activities in broken cell preparations. It appears unlikely that there is a direct interaction between alloxan and the enzyme molecules leading to irreversible changes. Alloxan may rather affect some metabolic factor essential for optimal enzyme function. The inhibition of glucose-stimulated increase in islet ATP content and adenylate energy charge in alloxan-treated islets suggests that such a factor might be dependent on intact ATP generation.</p>","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14126842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
beta-Adrenergic receptors and adenylate cyclase activity in murine lymphoid cell lines. 小鼠淋巴样细胞系的肾上腺素能受体和腺苷酸环化酶活性。
Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01
M Staehelin, P Müller, M Portenier, A W Harris
{"title":"beta-Adrenergic receptors and adenylate cyclase activity in murine lymphoid cell lines.","authors":"M Staehelin,&nbsp;P Müller,&nbsp;M Portenier,&nbsp;A W Harris","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>beta-Adrenergic receptors were determined in sixteen cultured tumor cell lines of T- and B-lymphoid derivation by the binding of the hydrophilic ligand [3H]CGP-12177. The number of receptors per cell varied from less than a hundred to over three thousand. Intracellular cAMP responses to isoproterenol and forskolin were measured for seven cell lines possessing a range of receptor numbers. No direct relationship was found between the isoproterenol-induced elevation of cAMP and the receptor number, and forskolin-induced increases in cAMP varied by more than 100-fold between cell lines. Adenylate cyclase activity was also measured in membrane preparations from the seven cell lines. The T-lymphoma line WEHI-22, the line with the highest receptor number (3700 per cell), showed very little response to any stimulatory agent. With the other four cell lines possessing receptors, the membrane-bound adenylate cyclase activity evoked by isoproterenol relative to that evoked by forskolin was found to be proportional to the number of receptors per cell. The number of receptors thus seems to determine the proportion of the inherent adenylate cyclase that can be activated by a beta-adrenergic agent. The levels of adenylate cyclase induced in membranes by GppNHp and by fluoride, relative to those induced by forskolin, were substantially higher with some cell lines than with others. These data and the results from WEHI-22 cells indicate that lymphoid cell lines differ in their content of functional C and N proteins.</p>","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14117713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adenylate cyclase activity as a function of forskolin concentration. 腺苷酸环化酶活性与福斯克林浓度的关系。
Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01
R Barber, T J Goka
{"title":"Adenylate cyclase activity as a function of forskolin concentration.","authors":"R Barber,&nbsp;T J Goka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The activation of adenylate cyclase by forskolin was investigated in terms of the dissociation model of guanyl nucleotide binding protein (Ns). It was demonstrated that the biphasic forskolin concentration-response of adenylate cyclase could be explained by the dissociation of the beta subunit. The equations developed from such a theoretical approach gave an accurate description of concentration response data from S49 cultured mouse lymphoma cells.</p>","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14118354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrastructural localization of cAMP-dependent protein kinase subunits in regenerating rat hepatocytes using immunogold electron microscopy. 免疫金电镜观察再生大鼠肝细胞camp依赖性蛋白激酶亚基的超微结构定位。
Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01
S P Squinto, D C Kelley-Geraghty, M R Kuettel, R A Jungmann
{"title":"Ultrastructural localization of cAMP-dependent protein kinase subunits in regenerating rat hepatocytes using immunogold electron microscopy.","authors":"S P Squinto,&nbsp;D C Kelley-Geraghty,&nbsp;M R Kuettel,&nbsp;R A Jungmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have used the indirect colloidal immunogold technique to examine the ultrastructural localization and distribution of the regulatory subunits (RI and RII) and catalytic subunit (C) of cAMP-dependent protein kinase (cA-PK) in hepatocyte nuclei during the prereplicative phase of rat liver regeneration. The technique allowed the localization of all three subunits of cA-PK in hepatocyte nuclei. Morphometric quantitation of the relative staining intensity of nuclear antigen indicated a marked increase of immunogold staining of nuclear RI and C 16 hours following partial hepatectomy. In contrast, staining of nuclear RII was not affected. These immunocytochemical observations correlate well with our previous data (Laks, M.S. et al. (1981) J. Biol. Chem. 256, 8775-8785) obtained by biochemical methods indicating a modulation of cA-PK activity after partial hepatectomy.</p>","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13722228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Forskolin stimulates bovine epididymal sperm motility and cyclic AMP levels. 福斯克林刺激牛附睾精子活力和循环AMP水平。
Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01
S Vijayaraghavan, D D Hoskins
{"title":"Forskolin stimulates bovine epididymal sperm motility and cyclic AMP levels.","authors":"S Vijayaraghavan,&nbsp;D D Hoskins","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Forskolin, a diterpene, has been reported to reversibly stimulate adenylate cyclase from a number of mammalian tissues. Adenylate cyclase preparations derived from sperm are reported to be both deficient in the guanine nucleotide subunit and insensitive to forskolin. Despite the latter observation, we report here that forskolin elevates cAMP levels in immature caput sperm, and initiates motility in the presence of \"permissive\" agents such as bicarbonate, procaine and dibucaine. In mature caudal sperm forskolin stimulates motility in a concentration dependent manner in the presence of the phosphodiesterase inhibitor IBMX but elevates cAMP levels only briefly before nucleotide levels return to control values. In addition, forskolin stimulates adenylate cyclase activity associated with plasma membrane preparations of caput sperm but not caudal sperm. This differential action of forskolin on these cell types could provide a basis for understanding the regulation of adenylate cyclase in both immature and mature bovine sperm.</p>","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13563272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple assay for cyclic adenosine 3':5'-monophosphate in human saliva. 人唾液中环腺苷3′:5′-单磷酸的简单测定。
Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01
U Glenert, A Geisler
{"title":"A simple assay for cyclic adenosine 3':5'-monophosphate in human saliva.","authors":"U Glenert,&nbsp;A Geisler","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Methods specially designed for the assay of cyclic adenosine 3':5'-monophosphate (cAMP) in human saliva have not previously been published. Methods for measurements in plasma or tissue preparations are inapplicable to saliva due to interference. This interference can be reduced by calibrating standard solutions with reagent blanks prepared from saliva pre-treated to remove cAMP (by charcoal adsorption). Further, human saliva contains only small amounts of cAMP (approximately 0.2 pmol/50 microliters saliva). The method presented provides the necessary sensitivity (lower detection limit: 0.02 pmol/50 microliters) obtained by adjustments of tracer and binding protein concentrations. The method is simple and has a comparatively great capacity for the number of test tubes to be assayed.</p>","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14132476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differences in the mode of action of 1-oleoyl-2-acetyl-glycerol and phorbol ester in platelet activation. 1-油基-2-乙酰基甘油和酚酯在血小板活化中的作用方式的差异。
Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01
B Ashby, M A Kowalska, E Wernick, M Rigmaiden, J L Daniel, J B Smith
{"title":"Differences in the mode of action of 1-oleoyl-2-acetyl-glycerol and phorbol ester in platelet activation.","authors":"B Ashby,&nbsp;M A Kowalska,&nbsp;E Wernick,&nbsp;M Rigmaiden,&nbsp;J L Daniel,&nbsp;J B Smith","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ADP-hydrolyzing enzyme apyrase inhibited platelet aggregation and phosphorylation of a 40,000 dalton platelet protein (P40) induced by 1-oleoyl-2-acetyl glycerol (OAG), indicating a dependence on secreted ADP. Apyrase also enhanced OAG-induced potentiation of forskolin or prostaglandin I2 activation of cyclic AMP formation in platelets. Cyclic AMP formation induced by OAG alone could be demonstrated in the presence of a phosphodiesterase inhibitor. Elevation of cyclic AMP level inhibits platelet aggregation so that secreted ADP may be required to inhibit OAG-activated adenylate cyclase for aggregation to proceed. In contrast, apyrase only partially affected phosphorylation of P40 and aggregation induced by the tumor promoter 12-0-tetradecanoyl phorbol-13-acetate (TPA). TPA caused marked inhibition of forskolin-stimulated cyclic AMP formation. TPA inhibition of cyclic AMP formation was largely reversed by apyrase, indicating that it was mainly due to release of ADP.</p>","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14132478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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