Journal of cyclic nucleotide and protein phosphorylation research最新文献

Identification of a rat liver cAMP-dependent protein kinase, type II, which binds DNA. 结合DNA的大鼠肝脏camp依赖性蛋白激酶II型的鉴定。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

J B Shabb, M R Miller

引用次数: 0

Demonstration of a high affinity component of glyceryl trinitrate induced vasodilatation in the bovine mesenteric artery. 证明高亲和力的三硝酸甘油成分诱导血管扩张在牛肠系膜动脉。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

J Ahlner, K L Axelsson, M E Ljusegren, N Grundström, R G Andersson

引用次数: 0

A simple method for the assay of Bordetella pertussis adenylate cyclase employing 31P nuclear magnetic resonance spectroscopy. 采用31P核磁共振波谱法测定百日咳杆菌腺苷酸环化酶。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

V Shirhatti, E Sokoloski, S Eng, S Hench, F Riccardi, G Krishna

引用次数: 0

Polyamine stimulation of protein phosphatase-2A from rat liver using a non-protein phosphoester substrate. 用非蛋白磷酸酯底物刺激大鼠肝脏蛋白磷酸酶2a的多胺研究。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

T Cornwell, P Mehta, S Shenolikar

引用次数: 0

Desensitization of the mammalian beta-adrenergic receptor: analysis of receptor redistribution on nonlinear sucrose gradients. 哺乳动物β -肾上腺素能受体的脱敏:非线性蔗糖梯度上受体再分布的分析。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

S Kassis, M Sullivan

{"title":"Desensitization of the mammalian beta-adrenergic receptor: analysis of receptor redistribution on nonlinear sucrose gradients.","authors":"S Kassis, M Sullivan","doi":"","DOIUrl":"","url":null,"abstract":"The distribution of beta-adrenergic receptors in lysates from several mammalian cell lines was analyzed on nonlinear sucrose density gradients before and after desensitization by isoproterenol. On the nonlinear gradients, the receptors in lysates from untreated HeLa, A431, S49 cyc- and C6 cells were well resolved into light and heavy density membrane fractions. In contrast, with the former three cell lines, there was very poor or no separation of the two peaks of receptors on linear sucrose gradients. With C6 cells, resolution was better on the nonlinear than on the linear gradient. In all cases, successful separation of the two density fractions of the receptor was achieved only when cells had been treated with concanavalin A prior to lysis. Adenylate cyclase activity cosedimented with the heavy membrane fraction of the receptor, and no activity was detected with the light fraction. After desensitization of adenylate cyclase by isoproterenol, there was a redistribution of the receptors to the light density fraction. This shift of receptors, but not desensitization, was prevented when cells were pretreated at 37 degrees C with concanavalin A prior to exposure to isoproterenol. Thus, sequestration of beta-adrenergic receptors away from the plasma membrane and adenylate cyclase to a lighter density membrane fraction appears to accompany, but may not be a prerequisite for desensitization in mammalian cells. This receptor redistribution, however, can be readily detected on nonlinear sucrose gradients.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14143196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Muscarinic cholinergic receptor-induced enhancement of PGE1-stimulated cAMP accumulation in neuroblastoma X glioma cells: prevention by pertussis toxin. 毒蕈碱胆碱能受体诱导的pge1刺激的神经母细胞瘤X胶质瘤细胞cAMP积累增强:百日咳毒素预防。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

J M Thomas, B B Hoffman

引用次数: 0

Role of cyclic GMP in gastrin secretion from rat antral mucosae in organ culture. 环GMP在器官培养大鼠胃窦粘膜分泌胃泌素中的作用。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

S A Lamprecht, B Schwartz, P Krugliak, H S Odes, R Guberman, J Krawiec

{"title":"Role of cyclic GMP in gastrin secretion from rat antral mucosae in organ culture.","authors":"S A Lamprecht, B Schwartz, P Krugliak, H S Odes, R Guberman, J Krawiec","doi":"","DOIUrl":"","url":null,"abstract":"Rat antral mucosae maintained for 6 h in organ culture responded to carbamylcholine with a significant increase in endogenous cyclic GMP production and gastrin secretion. The acetylcholine analogue exerted a stimulatory action within a defined concentration range: exposure of antral explants to carbachol concentrations greater than the optimal stimulatory dose was accompanied by a marked decrease in both cyclic GMP production and gastrin release. Exogenous 8-Br-cyclic GMP (1 mM) significantly augmented gastrin secretion into the culture media during 6-12 h culture periods. Cycloheximide (0.1 mM) and the Ca2+ channel-blocker verapamil (5 microM) prevented 8-Br-cyclic GMP from acting as a gastrin secretagogue. Addition of cyclic somatostatin-14 (0.1 mM) to culture media was attended by complete inhibition of 8-Br-cyclic GMP-stimulable gastrin secretion. These results provide evidence that cyclic GMP may play a mediatory role in the coupling of gastrin secretory processes to agonist stimulation. It would seem that the secretagogue action of 8-Br-cyclic GMP requires unabated Ca2+ transmembrane fluxes and protein biosynthesis. Since somatostatin-14 abrogates the stimulatory effect of 8-Br-cyclic GMP on antral gastrin secretion, it is surmised that the inhibitory tetradecapeptide acts at a locus (or loci) distal to domains involved in the actual generation of the cyclic nucleotide.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14013848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification of cyclic AMP- and cyclic GMP-dependent protein kinases from rat skeletal muscle. 大鼠骨骼肌中环AMP和环gmp依赖性蛋白激酶的纯化。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

R Johanson, A M Maddox, J Washington, A L Steiner

引用次数: 0

In vitro phosphorylation of 4-aminobutyrate aminotransferase by cAMP dependent protein kinase. cAMP依赖性蛋白激酶对4-氨基丁酸转氨酶的体外磷酸化作用。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

R K Carr, D Schlichter, C Spielholz, W D Wicks

{"title":"In vitro phosphorylation of 4-aminobutyrate aminotransferase by cAMP dependent protein kinase.","authors":"R K Carr, D Schlichter, C Spielholz, W D Wicks","doi":"","DOIUrl":"","url":null,"abstract":"Highly purified 4-aminobutyrate aminotransferase from pig brain is susceptible to phosphorylation by the purified cAMP-dependent protein kinase catalytic subunit. Up to 0.7 moles of phosphate from ATP-(gamma)-32P can be incorporated per mole of dimeric holoenzyme. Maximum phosphorylation was observed within about 90 minutes at 30 degrees C. Despite the extensive degree of phosphorylation observed, no kinetic property of the enzyme was perceptibly altered. Removal of cofactor had no detectable impact on the extent of phosphorylation but thermal inactivation of the enzyme increased and mild reduction with sodium borohydride decreased the phosphorylatability of the aminotransferase. It was possible to separate the enzyme into phospho and dephospho forms by the use of DEAE chromatography. Validation that the two fractions represented genuine aminotransferase was obtained by proteolytic peptide mapping. The phospho form of the enzyme was found to possess little or no aminotransferase activity while that of the dephospho form exhibited higher specific activity than the purified enzyme prior to phosphorylation. Furthermore, the dephospho form of the enzyme could not be detectably phosphorylated by reincubation with the kinase following DEAE chromatography unless it was subjected to thermal inactivation. The stoichiometry of phosphorylation of the fraction containing 32P from DEAE chromatography was approximately 1 mole/mole of dimer. These results suggest that the substrate for phosphorylation by the kinase is a form of the aminotransferase which is somehow inactivated during routine purification even when extensive precautions are taken to maximally preserve catalytic activity.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14819945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal regulation of adipocyte particulate "low Km" cAMP phosphodiesterase. 脂肪细胞颗粒“低Km”cAMP磷酸二酯酶的激素调节。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1986-01-01

V C Manganiello, C J Smith, A H Newman, K Rice, E Degerman, P Belfrage

引用次数: 0