Journal of cyclic nucleotide and protein phosphorylation research最新文献_第9页

A glutathione adduct of prostaglandin A1 acts intracellularly to elevate cyclic AMP by inhibiting its extrusion. 前列腺素A1的谷胱甘肽加合物在细胞内通过抑制环AMP的挤压而升高环AMP。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01

L E Heasley, J Azari, L L Brunton

引用次数: 0

Opiate and dopamine stimulate different GTPase in striatum: evidence for distinct modulatory mechanisms of adenylate cyclase. 阿片和多巴胺刺激纹状体中不同的GTPase:腺苷酸环化酶不同调节机制的证据。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01

F Tirone, M Parenti, A Groppetti

{"title":"Opiate and dopamine stimulate different GTPase in striatum: evidence for distinct modulatory mechanisms of adenylate cyclase.","authors":"F Tirone, M Parenti, A Groppetti","doi":"","DOIUrl":"","url":null,"abstract":"Previous studies demonstrated that opiate inhibition of adenylate cyclase (AC) in striatal membranes is related to an opiate-stimulated GTPase with a low Km. Dopamine (DA) also dose-dependently activates a high affinity GTPase, with a pattern of stimulation and a receptor selectivity (D1 type) similar to those observed in DA activation of striatal AC. Moreover, the DA- and the opiate-sensitive GTPase activities have different sensitivities to agents that affect the inhibition of AC, such as Na+ and N-ethylmaleimide (NEM), or the stimulation, such as cholera toxin (CTX). Thus, the impairment of opiate-dependent inhibition of AC in the absence of Na+ ions or after NEM pretreatment of the membranes is parallel with preferential impairment of the opiate-dependent GTPase. On the contrary, selective blocking by CTX of the DA-dependent GTPase leads to the enhancement of AC stimulation by DA. These results suggest that DA activation of striatal AC is related to a GTPase that is specifically stimulated by DA and is associated with the Ns protein. A distinct Ni protein seems to be responsible for the opiate effect on AC and GTPase.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":"10 4","pages":"327-39"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14126840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification of protein kinase C from bovine rod outer segments. 牛棒外段蛋白激酶C的纯化。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01

D J Kelleher, G L Johnson

{"title":"Purification of protein kinase C from bovine rod outer segments.","authors":"D J Kelleher, G L Johnson","doi":"","DOIUrl":"","url":null,"abstract":"Rod outer segments (ROS) from bovine retinae were found to have high levels of calcium/phospholipid dependent protein kinase (protein kinase C). Protein kinase C behaves as an extrinsic membrane protein and phosphorylates rhodopsin in a calcium-dependent manner. The abundance of protein kinase C in ROS is similar to that of rhodopsin kinase. Its ability to phosphorylate rhodopsin in ROS membranes suggests protein kinase C may play an important role in the regulation of signal transduction in the ROS. The limited set of extrinsic membrane proteins and abundance of protein kinase C makes this tissue an extremely useful source to purify protein kinase C. The extrinsic membrane protein fraction has 6-7 U protein kinase C activity per mg protein, and the enzyme is quite stable apparently due to the lack of proteases in the preparation. A procedure was developed using phosphatidylserine- and calcium-dependent binding of protein kinase C to phenyl-Sepharose in low ionic strength buffer to resolve protein kinase C and other calcium-binding proteins from the majority of extrinsic membrane proteins. Protein kinase C was eluted using EGTA, and peak fractions directly loaded onto a DEAE-cellulose column. The protein kinase C peak eluted from the ion-exchange column was pooled and had a specific activity greater than 1,000 nmol phosphate transferred to histone per min per mg protein with a recovery of 25 percent of the starting activity. The procedure to purify protein kinase C from ROS is simple and can be completed in one day.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":"10 6","pages":"579-91"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14136563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cholera toxin, cyclic AMP, and the firefly flash. 霍乱毒素，环磷酸腺苷，还有萤火虫。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01

J A Nathanson

引用次数: 0

Inhibition of cyclic AMP-dependent protein kinase-induced changes in the kinetic properties of hepatic pyruvate kinase by the specific cyclic AMP antagonist, the (Rp)-diastereomer of adenosine cyclic 3',5'-phosphorothioate. 特异性环AMP拮抗剂环3′，5′-硫代磷酸腺苷(Rp)-非对映体抑制环AMP依赖性蛋白激酶诱导肝丙酮酸激酶动力学性质的变化。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01

C J Dragland-Meserve, J D Rothermel, M J Houlihan, L H Botelho

{"title":"Inhibition of cyclic AMP-dependent protein kinase-induced changes in the kinetic properties of hepatic pyruvate kinase by the specific cyclic AMP antagonist, the (Rp)-diastereomer of adenosine cyclic 3',5'-phosphorothioate.","authors":"C J Dragland-Meserve, J D Rothermel, M J Houlihan, L H Botelho","doi":"","DOIUrl":"","url":null,"abstract":"The effects of the diastereomers of adenosine cyclic 3',5'-phosphorothioate, (Sp)- and (Rp)-cAMPS, on the kinetic properties of pyruvate kinase were studied in hepatocytes isolated from fed rats. Incubation of the cells with the cAMP-dependent protein kinase agonist, (Sp)-cAMPS, produced a concentration-dependent increase in S0.5 for phosphoenolpyruvate, but had no effect on Vmax. The (Sp)-cAMPS-treated enzyme was more sensitive to inhibition by alanine and ATP and, under the same conditions, was less responsive to activation by fructose-1,6-bisphosphate when assayed at a subsaturating phosphoenolpyruvate concentration. Incubation of the hepatocytes with only the cAMP-dependent protein kinase antagonist, (Rp)-cAMPS, produced no change in any kinetic parameters, but did suppress the (Sp)-cAMPS- or glucagon-induced increase in the S0.5 for phosphoenolpyruvate with IC50 values of 10 microM and 5 microM (Rp)-cAMPS. (Rp)-cAMPS is exerting an effect on the kinetic properties of pyruvate kinase through inhibition of cAMP-dependent protein kinase.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":"10 4","pages":"371-82"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14126845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guanine nucleotide inhibition of adenylate cyclase in a membrane fraction from Dictyostelium discoideum. 鸟嘌呤核苷酸对盘状盘基钢膜组分腺苷酸环化酶的抑制作用。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01

L Khachatrian, A Howlett, C Klein

引用次数: 0

Phosphorylation of the regulatory subunit of type I cyclic AMP-dependent protein kinase by its catalytic subunit. I型环amp依赖性蛋白激酶调控亚基的催化亚基磷酸化。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01

L C Huang, C Villar-Palasi, L E Kochevar, J P Charlton, L S King, C H Huang

引用次数: 0

Effect of forskolin on phosphorylation of a 25,000 Mr protein in perfused guinea pig hearts. 福斯克林对豚鼠灌注心脏25000 Mr蛋白磷酸化的影响。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01

L Fliegel, G I Drummond

引用次数: 0

Differential effects of in vitro ethanol on prostaglandin E1-sensitive adenylate cyclase from smooth muscle cells and platelets. 体外乙醇对平滑肌细胞和血小板中前列腺素e1敏感腺苷酸环化酶的影响。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01

M Noe, D Oliva, A Corsini, M Soma, R Fumagalli, S Nicosia

引用次数: 0

Activation of cyclic GMP-binding and cyclic AMP-specific phosphodiesterases of rat platelets by a mechanism involving cyclic AMP-dependent phosphorylation. 通过环amp依赖磷酸化的机制激活大鼠血小板环gmp结合和环amp特异性磷酸二酯酶。

Journal of cyclic nucleotide and protein phosphorylation research Pub Date : 1985-01-01

J Tremblay, B Lachance, P Hamet

{"title":"Activation of cyclic GMP-binding and cyclic AMP-specific phosphodiesterases of rat platelets by a mechanism involving cyclic AMP-dependent phosphorylation.","authors":"J Tremblay, B Lachance, P Hamet","doi":"","DOIUrl":"","url":null,"abstract":"We have previously reported that the cAMP-specific phosphodiesterase activity in washed rat platelets is increased by a short exposure of platelet suspension to PGE1 and 1-methyl-3-isobutyl-xanthine (MIX). We report here that the incubation of washed platelets with forskolin resulted in an increase in the binding of cGMP and the activity of cGMP-phosphodiesterase as well as that of cAMP-specific phosphodiesterase. As for PGE1, MIX potentiated the stimulatory effect of forskolin. The maximal activation of phosphodiesterases by forskolin and MIX occurred after 30 sec of incubation of platelets (with a slow decline thereafter). The activation of phosphodiesterases in intact platelets by forskolin occurred in parallel with the dissociation of a cAMP-dependent protein kinase. Prior incubation of a platelet supernatant with Mg-ATP and cAMP had only a slight effect on cAMP- or cGMP-phosphodiesterase activities, but the presence of MIX during the prior incubation, followed by appropriate dilution, greatly enhanced the activity of the two phosphodiesterases. The phosphodiesterase activation in vitro was inhibited by a non-hydrolysable analogue of ATP, AMP-PNP. Since the cGMP-binding phosphodiesterase activity is enhanced by the catalytic subunit of cAMP-dependent protein kinase in the presence of MIX and absence of cAMP, the effect of MIX cannot be explained in terms of the protection of cAMP from hydrolysis. It is possible that the xanthine increases the susceptibility of the cAMP-specific and cGMP-binding phosphodiesterases to phosphorylation.","PeriodicalId":15406,"journal":{"name":"Journal of cyclic nucleotide and protein phosphorylation research","volume":"10 4","pages":"397-411"},"PeriodicalIF":0.0,"publicationDate":"1985-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13558344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0