Journal of Clinical Pathology最新文献_第6页

Nancy histological index in ulcerative colitis: an interobserver study. 溃疡性结肠炎的南希组织学指标：一项观察者间研究。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-07-18 DOI: 10.1136/jcp-2025-210129

Melissa C Hutchings, Adrian C Bateman

引用次数: 0

Multinodular and vacuolating neuronal tumour: emphasis on expression of early and late neuronal immunomarkers. 多结节性和空泡性神经元肿瘤：早期和晚期神经元免疫标志物的表达。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-07-03 DOI: 10.1136/jcp-2025-210183

Sathyakumar Rima, Shilpa Rao, Anita Mahadevan, Thagadur Chickabasaviah Yasha, Arivazhagan Arimappamagan, Nishanth Sadashiva, Kavin K Devani, Karthik Kulanthaivelu

{"title":"Multinodular and vacuolating neuronal tumour: emphasis on expression of early and late neuronal immunomarkers.","authors":"Sathyakumar Rima, Shilpa Rao, Anita Mahadevan, Thagadur Chickabasaviah Yasha, Arivazhagan Arimappamagan, Nishanth Sadashiva, Kavin K Devani, Karthik Kulanthaivelu","doi":"10.1136/jcp-2025-210183","DOIUrl":"https://doi.org/10.1136/jcp-2025-210183","url":null,"abstract":"Aim: To analyse the expression of early, intermediate and late neuronal immunomarkers in multinodular and vacuolating neuronal tumour (MVNT) and understand the histogenesis of this rare tumour.Materials and methods: This is a retrospective study over a period of 5 years and included seven cases. Demographic, radiological and histopathological features were assessed. Immunohistochemistry was done for early (OLIG2, MAP2, Doublecortin), intermediate (alpha-internexin, neurofilament) and late neuronal immunomarkers (NeuN, synaptophysin).Results: All tumours were located in the cerebral hemisphere, mostly confined to temporal lobes with long-standing seizure as the most common symptom. On Magnetic resonance imaging (MRI), these tumours appeared mostly solid and were hypointense on T1 weighted image, hypointense to hyperintense on T2 weighted image. Six out of the seven cases showed nodular as well as diffuse growth pattern, located within deep cortical and superficial subcortical white matter. The nodules were composed of intermediate to large neuronal cells with prominent nucleoli and cytoplasmic vacuolation. The vacuolated neuronal cells showed immunolabelling for early neuronal immunomarkers and an autophagic immunomarker p62. The expression of late and intermediate neuronal immunomarkers was variable to absent. CD34 positive ramified neural elements were observed in the adjoining cortex of six cases. Follow-up data for four cases showed indolent behaviour.Conclusion: MVNT tumour cells consistently express early neuronal immunomarkers with variable expression of intermediate and late, suggesting maturation arrest early in the development. A combination of neuronal immunomarkers may be useful to diagnose these tumours when the classical histopathological pattern is not present.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Star of Paraform. 天台之星。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-07-02 DOI: 10.1136/jcp-2025-210193

Sarah Ruane, Nichola Gaunt, Jonathan Shanks

引用次数: 0

Assessment of PD-L1 expression and tumour infiltrating lymphocytes in early-stage non-small cell lung carcinoma with artificial intelligence algorithms. 用人工智能算法评估早期非小细胞肺癌的 PD-L1 表达和肿瘤浸润淋巴细胞。

IF 2 4区医学

Journal of Clinical Pathology Pub Date : 2025-06-19 DOI: 10.1136/jcp-2024-209766

Aida Molero, Susana Hernandez, Marta Alonso, Melina Peressini, Daniel Curto, Fernando Lopez-Rios, Esther Conde

{"title":"Assessment of PD-L1 expression and tumour infiltrating lymphocytes in early-stage non-small cell lung carcinoma with artificial intelligence algorithms.","authors":"Aida Molero, Susana Hernandez, Marta Alonso, Melina Peressini, Daniel Curto, Fernando Lopez-Rios, Esther Conde","doi":"10.1136/jcp-2024-209766","DOIUrl":"10.1136/jcp-2024-209766","url":null,"abstract":"Aims: To study programmed death ligand 1 (PD-L1) expression and tumour infiltrating lymphocytes (TILs) in patients with early-stage non-small cell lung carcinoma (NSCLC) with artificial intelligence (AI) algorithms.Methods: The study included samples from 50 early-stage NSCLCs. PD-L1 immunohistochemistry (IHC) stained slides (clone SP263) were scored manually and with two different AI tools (PathAI and Navify Digital Pathology) by three pathologists. TILs were digitally assessed on H&E and CD8 IHC stained sections with two different algorithms (PathAI and Navify Digital Pathology, respectively). The agreement between observers and methods for each biomarker was analysed. For PD-L1, the turn-around time (TAT) for manual versus AI-assisted scoring was recorded.Results: Agreement was higher in tumours with low PD-L1 expression regardless of the approach. Both AI-powered tools identified a significantly higher number of cases equal or above 1% PD-L1 tumour proportion score as compared with manual scoring (p=0.00015), a finding with potential therapeutic implications. Regarding TAT, there were significant differences between manual scoring and AI use (p value <0.0001 for all comparisons). The total TILs density with the PathAI algorithm and the total density of CD8+ cells with the Navify Digital Pathology software were significantly correlated (τ=0.49 (95% CI 0.37, 0.61), p value<0.0001).Conclusions: This preliminary study supports the use of AI algorithms for the scoring of PD-L1 and TILs in patients with NSCLC.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"456-464"},"PeriodicalIF":2.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12322406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mounting agents with low toxicity and with fast curing time for digital pathology in the intraoperative frozen section laboratory. 用于术中冰冻切片实验室数字病理学的低毒性、快速固化的装片剂。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-06-19 DOI: 10.1136/jcp-2024-209417

Mette Wessel Frandsen, Lone Bojesen, Sys Johnsen, Lene Buhl Riis, Julie Smith

{"title":"Mounting agents with low toxicity and with fast curing time for digital pathology in the intraoperative frozen section laboratory.","authors":"Mette Wessel Frandsen, Lone Bojesen, Sys Johnsen, Lene Buhl Riis, Julie Smith","doi":"10.1136/jcp-2024-209417","DOIUrl":"10.1136/jcp-2024-209417","url":null,"abstract":"Aims: In intraoperative frozen tissue section laboratories (FS laboratories) conventional practice for mounting coverslips on tissue slides involves the use of xylene-based mounting agents, such as Pertex. However, toxic vapours pose a risk to biomedical laboratory scientists (BLS) and pathologists who handle the wet slides to provide fast and urgent diagnostic results to surgeons during operations. Our study aims to evaluate non-toxic mounting agents to substitute Pertex, preferably with a fast curing time suitable for the demands of the new digital era in pathology.Methods: Five non-toxic mounting agents were purchased and tested through six different protocols and compared to xylene-based Pertex as our gold standard. With light microscopy, tissue slides were quality assessed by BLS. Mounting agents, which were evaluated comparable to Pertex, were also evaluated by a pathologist, hence scanned digitally and re-evaluated.Results: The protocols for Eukitt UV, Eukitt UV R-1 and Eukitt UV R-2 had significantly more artefacts (bubbles) compared to gold standard Pertex (p<0.0001, p=0.004 and p<0.0001, respectively) and assessed inadequate as replacements. Neo-Mount and Tissue Mount were assessed applicable regarding quality, but curing times were long. Tek Select UV showed promising results in both quality and fast curing time (protocol was <2 min).Conclusions: Toxic mounting agents need replacement to health guard professionals, and also digital pathology is revolutionising laboratories. A digitalized FS laboratory requires fast dry/cured slides for digital scanning. Therefore, a substitute for the FS laboratory should have the qualities of being non-toxic to handle and having a fast curing time, and a UV-based mounting agent may solve both requirements.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"495-502"},"PeriodicalIF":2.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary melanoma of the urinary tract: a clinicopathological study of cases and literature review. 泌尿道原发性黑色素瘤：病例临床病理学研究与文献综述。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-06-19 DOI: 10.1136/jcp-2024-209684

Lisha Wang, Mohammed Wali, Yue Sun

引用次数: 0

Mutational profile dynamics in follicular lymphoma and large cell transformation. 滤泡性淋巴瘤和大细胞转化的突变谱动态。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-06-19 DOI: 10.1136/jcp-2024-209880

Eva A M Hesius, Wendy B C Stevens, James P Stewart, Leonie I Kroeze, Ellen van der Spek, Djamila Issa, Peet Nooijen, Jeroen Luijks, David Gonzalez, Patricia J T A Groenen, Nicole M A Blijlevens, Annemiek B van Spriel, Michiel van den Brand

{"title":"Mutational profile dynamics in follicular lymphoma and large cell transformation.","authors":"Eva A M Hesius, Wendy B C Stevens, James P Stewart, Leonie I Kroeze, Ellen van der Spek, Djamila Issa, Peet Nooijen, Jeroen Luijks, David Gonzalez, Patricia J T A Groenen, Nicole M A Blijlevens, Annemiek B van Spriel, Michiel van den Brand","doi":"10.1136/jcp-2024-209880","DOIUrl":"10.1136/jcp-2024-209880","url":null,"abstract":"Aims: Follicular lymphoma (FL) is characterised by significant heterogeneity in both the clinical trajectories and the molecular profiles. This study aimed to investigate clonal dynamics in FL by analysing mutation profiles at various time points during the disease course including at histological transformation (HT), to gain insight into the mutational changes over time.Methods: We retrospectively analysed 76 biopsies from 25 patients, including 13 cases with three or more FL biopsies and 12 cases with subsequent HT. Hybrid capture-based Next-Generation Sequencing (NGS) with the EuroClonality-NGS DNA capture (EuroClonality-NDC) assay was used to examine clonal rearrangements and mutations.Results: A total of 204 (potentially) pathogenic mutations were identified. Only 40% of mutations remained stably present during a median follow-up period of 139 months (range 9-198). KMT2D and CREBBP were the most frequently mutated genes at diagnosis, exhibiting relative stability in follow-up biopsies. Conversely, EZH2 displayed a dynamic pattern of mutations gained and lost during the disease course. At HT, pathogenic mutations affecting B2M, MYC and TP53 emerged. Changes in mutational burden were observed in both FL-sequential and diagnosis-transformation cohorts, with more pronounced changes in the latter.Conclusions: This real-world study provides insights into the complex molecular pathogenesis of FL and HT. As targeted therapies emerge as treatment modalities, mutational profiles could influence treatment decisions in the future. Therefore, recognising the significant changes occurring in the mutational landscape of FL throughout the disease course is crucial.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"473-482"},"PeriodicalIF":2.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frequency and clinicopathologic features of renal low-grade oncocytic tumour and eosinophilic vacuolated tumour: reclassification of 605 eosinophilic tumours including patients managed with active surveillance. 肾脏低级别肿瘤细胞瘤和嗜酸性空泡瘤的发病率和临床病理特征：对 605 例嗜酸性肿瘤（包括接受主动监测的患者）进行重新分类。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-06-19 DOI: 10.1136/jcp-2024-209711

Roselyne Choiniere, Shifaa' Al Qa'qa', Carol C Cheung, Antonio Finelli, Susan Prendeville

{"title":"Frequency and clinicopathologic features of renal low-grade oncocytic tumour and eosinophilic vacuolated tumour: reclassification of 605 eosinophilic tumours including patients managed with active surveillance.","authors":"Roselyne Choiniere, Shifaa' Al Qa'qa', Carol C Cheung, Antonio Finelli, Susan Prendeville","doi":"10.1136/jcp-2024-209711","DOIUrl":"10.1136/jcp-2024-209711","url":null,"abstract":"Aims: Low-grade oncocytic tumour (LOT) and eosinophilic vacuolated tumour (EVT) are recently described emerging entities, which demonstrate distinct features but are not yet recognised as separate neoplasms in the fifth WHO classification. Published series to date have been largely multi-institutional and based on surgically resected tumours. This study aims to determine the frequency, clinicopathologic features and outcome of LOT and EVT in a single institutional series of oncocytic/eosinophilic renal neoplasms, including patients managed with active surveillance and non-surgical intervention.Methods and results: Cases were identified from a consecutive institutional series of in-house renal tumours diagnosed on biopsy and/or nephrectomy (2003-2023). Tumours with a diagnosis or differential diagnosis of oncocytoma, chromophobe renal cell carcinoma or oncocytic neoplasm not otherwise specified (including LOT, EVT and tumours with overlapping hybrid features) were retrospectively reviewed and classified/reclassified.In total, 605 oncocytic/eosinophilic renal neoplasms were reviewed, among which 33 LOT (5.5%) and 5 EVT (0.8%) were identified. LOT were CK7+, CD117- and GATA3+ (94%). EVT were CD117+, CK7 focal+ (80%) and cathepsin K+ (80%). At the median follow-up of 34 months (range 2-253) and 56 months (range 8-90) for LOT and EVT, respectively, there was no evidence of recurrence following ablation/surgical resection, metastasis or death from disease for all patients, including the 22 managed with active surveillance (20 LOT and 2 EVT).Conclusions: LOT and EVT comprised a minority of oncocytic renal neoplasms in this series. We report a large institutional series including patients managed non-surgically, with no adverse outcome, adding to the existing literature indicating a benign outcome.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"436-442"},"PeriodicalIF":2.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LGR5 as a diagnostic marker for dysplasia in serrated polyps. LGR5作为锯齿状息肉发育不良的诊断标志物。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-06-19 DOI: 10.1136/jcp-2024-209856

Osman Yilmaz, Kshtij Arora, Soo Hyun Lee, Sahar Hosseini, Feidi Chen, Nandan Padmanabha, George Eng, Kanchan Kantekure, Omer Yilmaz, Vikram Deshpande

{"title":"LGR5 as a diagnostic marker for dysplasia in serrated polyps.","authors":"Osman Yilmaz, Kshtij Arora, Soo Hyun Lee, Sahar Hosseini, Feidi Chen, Nandan Padmanabha, George Eng, Kanchan Kantekure, Omer Yilmaz, Vikram Deshpande","doi":"10.1136/jcp-2024-209856","DOIUrl":"10.1136/jcp-2024-209856","url":null,"abstract":"Aims: WNT signalling pathway dysregulation is often a critical early component in colorectal neoplasia, particularly the chromosomal instability pathway. Using two WNT reporters, LGR5 and AXIN2, we sought to assess whether these polyps demonstrate predictable expression patterns and if these patterns show diagnostic value.Methods: We evaluated 23 adenomas (TA), 23 sessile serrated lesions (SSLs), 14 SSL with dysplasia and 38 traditional serrated adenomas (TSA). Chromogenic in situ hybridisation stains (ISH) for LGR5 and AXIN2 were performed. Reactivity was defined as strong, intermediate or weak. Upper third crypt reactivity was defined as full-thickness staining. Accentuation within ectopic crypts (ECF) was recorded.Results: TAs (91%) showed strong reactivity and full-thickness staining with LGR5. TSAs showed full-thickness and weak to intermediate LGR5 reactivity (79%) and ECF with LGR5 accentuation was exclusively seen in TSA. SSL showed weak LGR5 reactivity confined to the basal crypt region (100%). SSL with dysplasia also showed weak or intermediate (100%) LGR5 reactivity, but the reactivity pattern was full thickness (88%). AXIN2 expression parallels LGR5 expression (Pearson coefficient=0.63) regarding signal intensity for the examined polyp groups.Conclusions: Qualitative and quantitative differences in AXIN2 and LGR5 expression assist in the diagnosis of SSL with dysplasia.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"465-472"},"PeriodicalIF":2.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142949551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pancreatic neuroendocrine tumours: a comparison of cytological classification systems. 胰腺神经内分泌肿瘤：细胞学分类系统比较。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-06-19 DOI: 10.1136/jcp-2024-209507

Lauren Ackroyd, Matthew Hanks, Andrei Bancu, Marium Khan, Saira Sajid, Dileep N Lobo, Abed M Zaitoun

{"title":"Pancreatic neuroendocrine tumours: a comparison of cytological classification systems.","authors":"Lauren Ackroyd, Matthew Hanks, Andrei Bancu, Marium Khan, Saira Sajid, Dileep N Lobo, Abed M Zaitoun","doi":"10.1136/jcp-2024-209507","DOIUrl":"10.1136/jcp-2024-209507","url":null,"abstract":"Aims: Cytological classification systems provide a standardised interpretation framework for reporting cytological specimens. Three well-known classification systems can be applied when reporting pancreatic cytology. This study aimed to compare the accuracy of these classification systems (C1-C5 system, the Papanicolaou system and the WHO classification) for the assessment of pancreatic neuroendocrine lesions.Methods: We analysed 73 pancreatic neuroendocrine tumour resections, 49 of which had corroborative cytology available, reported over a 12-year period, at a single UK tertiary referral centre. Each cytology case was classified using the aforementioned systems. The final tumour grade allocated at resection was used to assess and compare the accuracy of each cytological classification system.Results: Cytological assessment accurately reported 77.6% of neuroendocrine lesions as category IVB (neoplastic - other) on Papanicolaou grading, 77.6% as C5 (malignant) lesions and 85.7% as VII (malignant) on WHO grading. 74.3% of resected tumours were grade 1, 17.1% grade 2 and 8.6% grade 3. Complete resection was achieved in 80.8% of cases.Conclusions: The results demonstrated that the WHO classification appeared to provide reduced ambiguity when compared with both 'C' and Papanicolaou classification systems; with a lower proportion of cases being classified as suspicious of malignancy as opposed to malignant. The Papanicolaou system was able to supersede the other two systems through its ability to distinguish neuroendocrine tumours from more aggressive entities such as pancreatic adenocarcinoma, thus, offering flexibility in management while still retaining a similar level of accuracy to the WHO classification system in distinguishing benign from malignant lesions.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"449-455"},"PeriodicalIF":2.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140945001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0