Journal of Clinical Pathology最新文献_第6页

Low-positive controls for monitoring progesterone receptor immunohistochemical staining. 监测孕酮受体免疫组化染色的低阳性对照。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-01-16 DOI: 10.1136/jcp-2024-209902

Yu-Hsun Lin, Jen-Fan Hang, Ching-Fen Yang, Chih-Yi Hsu

{"title":"Low-positive controls for monitoring progesterone receptor immunohistochemical staining.","authors":"Yu-Hsun Lin, Jen-Fan Hang, Ching-Fen Yang, Chih-Yi Hsu","doi":"10.1136/jcp-2024-209902","DOIUrl":"https://doi.org/10.1136/jcp-2024-209902","url":null,"abstract":"Aims: Progesterone receptor (PR) is a crucial prognostic marker in breast cancer. However, achieving consistent results in PR immunohistochemistry (IHC) remains challenging due to the lack of well-defined low-positive controls. This study aimed to identify benign tissues with consistent low-level PR expression to serve as ideal controls for IHC.Methods: We evaluated PR expression in the squamous epithelium of the uterine cervix, nipple smooth muscle and pancreatic islets. QuPath digital image analysis was employed to compare the intensity and quantity of PR staining in target cells within a 2×2 mm area.Results: The squamous epithelium of the secretory phase cervix, nipple smooth muscle and pancreatic islets displayed appreciable weak PR expression, with mean values of 73, 55 and 60 cells, respectively. Notably, 62% (8/13) of the 2×2 mm areas in the atrophic cervix were completely negative for PR expression. The coefficients of variation for weak PR-expressing cells in pancreatic islets (57.4%) and nipple smooth muscle (65.0%) were lower than those observed in the cervix (96.2%-222.0%). The squamous epithelium of the cervix, especially during the secretory phase, exhibited weak positivity confined to the basal layers, providing another viable control option. However, variations in PR expression may be influenced by physiological factors, such as hormonal fluctuations.Conclusions: Pancreatic islets and nipple smooth muscle, with their consistent low-level PR expression, offer a promising solution to the challenges associated with PR IHC. This approach may help minimise variations resulting from differing staining methods across laboratories.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SARS-CoV-2 seropositivity amongst healthcare workers in South Africa during the Omicron wave: natural infection versus vaccination. 欧米克朗波期间南非卫生保健工作者中SARS-CoV-2血清阳性：自然感染与疫苗接种

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-01-16 DOI: 10.1136/jcp-2024-209722

Daniel J Hoffmann, Pieter W A Meyer, Catherine M Worsley, Mieke A van der Mescht, A Visser, Tahir S Pillay

{"title":"SARS-CoV-2 seropositivity amongst healthcare workers in South Africa during the Omicron wave: natural infection versus vaccination.","authors":"Daniel J Hoffmann, Pieter W A Meyer, Catherine M Worsley, Mieke A van der Mescht, A Visser, Tahir S Pillay","doi":"10.1136/jcp-2024-209722","DOIUrl":"https://doi.org/10.1136/jcp-2024-209722","url":null,"abstract":"Aims: Concerns over population-level immunity have been heightened with each successive wave of COVID-19, prompting questions about whether it is primarily derived from vaccination efforts or from previous natural infections with the virus. We wished to determine the seroprevalence of SARS-CoV-2 antibodies among healthcare workers (HCWs) in Pretoria (Tshwane), South Africa, and to establish whether they were derived from vaccination or natural infection.Methods: Serum samples were collected from HCWs during the fourth wave of COVID-19 between 1 December 2021 and 13 March 2022. The samples were tested using the Abbott SARS-CoV-2 Spike IgG (S-IgG), IgM (S-IgM) and the SARS-CoV-2 Nucleocapsid IgG (NC-IgG) kits.Results: Of the 221 participants, 76% (n=168) were women and 24% (n=53) were men. A total of 96.4% (n=213) of the participants were vaccinated. Natural infection-derived antibodies were detected in 23% (n=51) of participants, and vaccine-derived antibodies in 74% (n=164) of the HCWs.Conclusions: Even after three waves of COVID-19, HCWs derived most of their detectable antibodies from vaccination. Vaccination remains an essential tool to protect HCWs and patients from SARS-CoV-2 infection.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rethinking alcoholic foamy degeneration of the liver: a study of nine cases highlighting complex pathological findings. 对肝脏酒精性泡沫变性的再思考：九例复杂病理结果的研究。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-01-16 DOI: 10.1136/jcp-2024-209939

Dhaarica Jeyanesan, Alessandro Antonello, Mary Cannon, Yoh Zen

{"title":"Rethinking alcoholic foamy degeneration of the liver: a study of nine cases highlighting complex pathological findings.","authors":"Dhaarica Jeyanesan, Alessandro Antonello, Mary Cannon, Yoh Zen","doi":"10.1136/jcp-2024-209939","DOIUrl":"https://doi.org/10.1136/jcp-2024-209939","url":null,"abstract":"Aims: To reveal clinicopathological characteristics of alcoholic foamy degeneration (AFD)-an uncommon form of alcoholic liver injury.Methods: Clinicopathological features of AFD (n=9) were examined in comparison to those of severe alcoholic hepatitis (SAH; n=12).Results: Patients with AFD presented with either biochemical liver dysfunction (n=1) or clinical jaundice (n=8). One case had undergone liver transplantation for alcohol-related cirrhosis and hepatocellular carcinoma 2 years and 3 months before presentation. AFD cases were histologically classified into three groups. The non-jaundiced case had mixed macro- and microvesicular bland steatosis. Seven jaundiced cases showed more complex microscopic features with lobular inflammation, acidophilic bodies, cholestasis and lobular distortion. Hepatocytes were pleomorphic, some extensively enlarged with clear cytoplasm, somewhat resembling ballooning degeneration; however, it was mainly due to accumulated lipid droplets ('pseudoballooning'). The remaining case also had predominant changes of AFD, but a few foci showed classical ballooning hepatocytes and Mallory-Denk bodies, in keeping with mixed AFD and steatohepatitis. When compared with patients with SAH, those with AFD had lower white blood cell and neutrophil counts and higher cholesterol levels (all p<0.001). On imaging, ascites and varices were less common in AFD than in SAH (11% vs 75%, p=0.014; 0% vs 67%, p=0.008, respectively). All seven patients with AFD who successfully abstained from alcohol experienced rapid improvement in liver function.Conclusions: Microscopic findings of AFD are more complex than currently thought, and some cases may be mistaken for steatohepatitis. AFD may also develop in conjunction with steatohepatitis or following liver transplantation.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-molecule localisation microscopy (SMLM) is feasible in human and animal formalin fixed paraffin embedded (FFPE) tissues in medical renal disease. 单分子定位显微镜（SMLM）在医学肾脏病人、动物的福尔马林固定石蜡包埋（FFPE）组织中是可行的。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-01-13 DOI: 10.1136/jcp-2024-209853

Scarlet F Brockmoeller, Hayley Slaney, Alistair Curd, Aurora Bono, James H Felce, Deep Arora, Andrew Lewington, Andras G Miklosi, Philip Quirke

{"title":"Single-molecule localisation microscopy (SMLM) is feasible in human and animal formalin fixed paraffin embedded (FFPE) tissues in medical renal disease.","authors":"Scarlet F Brockmoeller, Hayley Slaney, Alistair Curd, Aurora Bono, James H Felce, Deep Arora, Andrew Lewington, Andras G Miklosi, Philip Quirke","doi":"10.1136/jcp-2024-209853","DOIUrl":"https://doi.org/10.1136/jcp-2024-209853","url":null,"abstract":"Aims: Establishment of a protocol for routine single-molecule localisation microscopy (SMLM) imaging on formalin fixed paraffin embedded (FFPE) tissue using medical renal disease including minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS).Methods: Protocol for normal and diseased renal FFPE tissue was developed to investigate the clinical diagnostic potential of SMLM. Antibody concentrations were determined for confocal microscopy and transferred to SMLM. Different fixatives and lengths of fixation were studied. To reduce autofluorescence, additional quenching and UV bleaching steps were compared. Optimal SMLM acquisition settings were established. SMLM data were imaged, digitally captured, stored, visually inspected and analysed quantitatively.Results: Protocol was established on normal renal FFPE tissue and then applied to clinical diseased tissue with single and multiple markers. Antibodies against key diagnostic proteins including podocin, nephrin, collagen, laminin, synaptopodin, CD31, IgG, IgM and IgA antibodies were established for MCD, FSGS and immune-mediated renal disease. We found important characteristic differences in the renal diseases listed above.Conclusions: We established a routine super-resolution microscopy protocol for clinical FFPE material on medical renal biopsies, which could visualise fluorescently labelled proteins in all glomeruli present with a precision of approximately 10-20 nm, with a turnaround under 48 hours. We visualised and quantitated specific protein distributions in different conditions. SMLM opens subcellular microscopy in FFPE to histopathologists on routine FFPE tissue, which can in the future be an adjunct and, in some aspects, a rapid superior alternative to electron microscopy.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

'Sneaky' uninflamed oesophageal candidiasis: morphological clues and comparison with candidiasis associated with inflammation. 偷偷摸摸 "的无炎症食道念珠菌病：形态线索以及与炎症相关念珠菌病的比较。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-01-11 DOI: 10.1136/jcp-2024-209908

Yasamin Mirzabeigi, Turky Alkhatery, Amr Abulaban, Felipe Ruiz Casas, Elizabeth Anne Montgomery

{"title":"'Sneaky' uninflamed oesophageal candidiasis: morphological clues and comparison with candidiasis associated with inflammation.","authors":"Yasamin Mirzabeigi, Turky Alkhatery, Amr Abulaban, Felipe Ruiz Casas, Elizabeth Anne Montgomery","doi":"10.1136/jcp-2024-209908","DOIUrl":"https://doi.org/10.1136/jcp-2024-209908","url":null,"abstract":"Aims: Candida esophagitis is usually readily identified on routine H&E-stained sections as the infection typically presents with prominent acute inflammation as a clue to search for organisms. However, in some cases, inflammation is absent, and detection of organisms relies on the observation of zones exhibiting parakeratosis with a delicate 'flaky' appearance. Our study aimed to establish a correlation between the histomorphology of oesophageal candidiasis and an associated clinical profile.Methods: We reviewed 53 sequential biopsy specimens from patients with Candida esophagitis collected over 1 year. Biopsies were assessed for acute inflammation, intraepithelial lymphocytosis and lymphoid aggregates. Patients' medical records were reviewed for data on age, gender, race, immune status, smoking, corticosteroid use, HIV status and organ transplantation history. Correlations between these factors and histomorphological patterns were assessed using test.Results: Of the 53 biopsies, 20 lacked acute inflammation and 33 had it. 15 biopsies showed both acute and lymphoid inflammation and 5 showed lymphocytosis only. Among 16 smokers, 6 (37%) had acute inflammation and 10 (63%) had parakeratosis. In non-smokers, 24 (71%) had acute inflammation and 10 (29%) had parakeratosis. A significant correlation was found between smoking and absence of acute neutrophilic infiltration (p=0.025), but no other clinical factor was associated with inflammatory patterns.Conclusions: Candida esophagitis can be uninflamed with 'flaky' parakeratosis or associated with acute inflammation or lymphocytosis with or without neutrophilic infiltration. Inflammation was often absent in smokers, suggesting synergistic local immunosuppressive effect is this overall immunosuppressed population.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calcified chondroid mesenchymal neoplasm: a clinicopathological and molecular analysis. 钙化软骨间充质瘤：临床病理和分子分析。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-01-11 DOI: 10.1136/jcp-2024-209806

Xiaolong Feng, Suxia Wang, Jiacong Wei, Weihua Li, Shun Wang, Peng Guo, Changyuan Guo, Weiwei Hao, Hongtian Dai, Lihua Gong

{"title":"Calcified chondroid mesenchymal neoplasm: a clinicopathological and molecular analysis.","authors":"Xiaolong Feng, Suxia Wang, Jiacong Wei, Weihua Li, Shun Wang, Peng Guo, Changyuan Guo, Weiwei Hao, Hongtian Dai, Lihua Gong","doi":"10.1136/jcp-2024-209806","DOIUrl":"https://doi.org/10.1136/jcp-2024-209806","url":null,"abstract":"Aims: Calcified chondroid mesenchymal neoplasm (CCMN) is a recently identified category of soft tissue neoplasms defined by cartilage or cartilaginous matrix formation and FN1 gene fusions. Its rarity and similarities to other soft tissue tumours pose diagnostic challenges. This study aims to deepen understanding of CCMN, highlighting molecular pathology's role in diagnosis to reduce misdiagnosis, overdiagnosis and overtreatment.Methods: We conducted a clinicopathological analysis of five newly identified CCMN cases and reviewed 87 cases documented in PubMed. Next-generation sequencing was used to detect molecular alterations, while clinical, radiological and histopathological features were extensively reviewed.Results: CCMN typically affects adults, presenting as a slow-growing, painless mass in soft tissue. Histologically, CCMN exhibits a chondroid matrix with variable calcification. Molecular analyses in our cases identified FN1::FGFR1, FN1::FGFR2 and FN1::TEK fusions. Review of the 87 cases revealed consistent clinical, imaging and molecular profiles, underscoring CCMN's distinct characteristics.Conclusions: CCMN should be considered in the differential diagnosis of soft tissue tumours with chondroid and calcified components. Detecting FN1 gene fusions aids in distinguishing CCMN from morphologically similar tumours.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LGR5 as a diagnostic marker for dysplasia in serrated polyps. LGR5作为锯齿状息肉发育不良的诊断标志物。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-01-09 DOI: 10.1136/jcp-2024-209856

Osman Yilmaz, Kshtij Arora, Soo Hyun Lee, Sahar Hosseini, Feidi Chen, Nandan Padmanabha, George Eng, Kanchan Kantekure, Omer Yilmaz, Vikram Deshpande

{"title":"LGR5 as a diagnostic marker for dysplasia in serrated polyps.","authors":"Osman Yilmaz, Kshtij Arora, Soo Hyun Lee, Sahar Hosseini, Feidi Chen, Nandan Padmanabha, George Eng, Kanchan Kantekure, Omer Yilmaz, Vikram Deshpande","doi":"10.1136/jcp-2024-209856","DOIUrl":"https://doi.org/10.1136/jcp-2024-209856","url":null,"abstract":"Aims: WNT signalling pathway dysregulation is often a critical early component in colorectal neoplasia, particularly the chromosomal instability pathway. Using two WNT reporters, LGR5 and AXIN2, we sought to assess whether these polyps demonstrate predictable expression patterns and if these patterns show diagnostic value.Methods: We evaluated 23 adenomas (TA), 23 sessile serrated lesions (SSLs), 14 SSL with dysplasia and 38 traditional serrated adenomas (TSA). Chromogenic in situ hybridisation stains (ISH) for LGR5 and AXIN2 were performed. Reactivity was defined as strong, intermediate or weak. Upper third crypt reactivity was defined as full-thickness staining. Accentuation within ectopic crypts (ECF) was recorded.Results: TAs (91%) showed strong reactivity and full-thickness staining with LGR5. TSAs showed full-thickness and weak to intermediate LGR5 reactivity (79%) and ECF with LGR5 accentuation was exclusively seen in TSA. SSL showed weak LGR5 reactivity confined to the basal crypt region (100%). SSL with dysplasia also showed weak or intermediate (100%) LGR5 reactivity, but the reactivity pattern was full thickness (88%). AXIN2 expression parallels LGR5 expression (Pearson coefficient=0.63) regarding signal intensity for the examined polyp groups.Conclusions: Qualitative and quantitative differences in AXIN2 and LGR5 expression assist in the diagnosis of SSL with dysplasia.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142949551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic utility of ERG immunostaining in dermatofibroma: be aware of ERG expression in cellular neurothekeoma. ERG免疫染色在皮肤纤维瘤中的诊断价值：注意细胞性神经瘤中ERG的表达。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2025-01-08 DOI: 10.1136/jcp-2024-209913

Fleur Cordier, Liesbeth Ferdinande, Jo Van Dorpe, David Creytens

引用次数: 0

c-Met immunohistochemistry as reflex test at diagnosis for non-small cell lung cancer: a real-world experience from a monocentric case series. c-Met免疫组织化学作为诊断癌症非小细胞肺癌的反射测试：来自单中心病例系列的真实经验。

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2024-12-18 DOI: 10.1136/jcp-2023-209202

Christophe Bontoux, Veronique Hofman, Milissa Abboute, Virginie Lespinet-Fabre, Salomé Lalvée, Samantha Goffinet, Olivier Bordone, Elodie Long-Mira, Sandra Lassalle, Florent Murcy, Guylène Rignol, Simon Heeke, Marius Ilie, Paul Hofman

{"title":"c-Met immunohistochemistry as reflex test at diagnosis for non-small cell lung cancer: a real-world experience from a monocentric case series.","authors":"Christophe Bontoux, Veronique Hofman, Milissa Abboute, Virginie Lespinet-Fabre, Salomé Lalvée, Samantha Goffinet, Olivier Bordone, Elodie Long-Mira, Sandra Lassalle, Florent Murcy, Guylène Rignol, Simon Heeke, Marius Ilie, Paul Hofman","doi":"10.1136/jcp-2023-209202","DOIUrl":"10.1136/jcp-2023-209202","url":null,"abstract":"Aims: Recent clinical trials have shown promising results with drugs targeting the hepatocyte growth factor receptor (c-Met) for advanced non-small cell lung cancers overexpressing c-Met. We assessed reflex testing of c-Met immunohistochemistry (IHC) at diagnosis for NSCLC in the real-world.Methods: We retrospectively collected clinical, pathological and molecular data of cases diagnosed with NSCLC in our institution from January 2021 to June 2023. We performed c-Met IHC (SP44 clone) and scored the expression using a H-score and a three-tier classification.Results: 391 cases with interpretable c-Met IHC staining were included. The median age at diagnosis was 70 years (range 25-89 years) including 234 males (male/female ratio 1:5). 58% of the samples came from surgical resections, 35% from biopsies and 8% from cytological procedures. 52% of cases were classified as c-Met-positive (H-score≥150) and 19% were classified as c-Methigh (≥50%, 3+). 43% of the c-Metneg presented with lymph node and/or visceral metastases at diagnosis vs 55% for c-Methigh (p=0.042). 23% of the adenocarcinomas showed c-Methigh expression vs 3% for squamous cell carcinomas (p=0.004). 27% of the c-Metneg cases had a high PD-L1 expression vs 58% of c-Methigh cases (p<0.001). MET ex14 skipping was present in 8% of the c-Methigh cases.Conclusions: Systematic c-Met testing in daily routine for NSCLC patients is feasible, highlighting a potential correlation with clinicopathological and molecular features.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"35-41"},"PeriodicalIF":2.5,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71521612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological features of liver disease development in the Atp7b^-/- mouse: a model of Wilson's disease. Atp7b-/-小鼠肝脏疾病发展的组织学特征:威尔逊病模型

IF 2.5 4区医学

Journal of Clinical Pathology Pub Date : 2024-12-18 DOI: 10.1136/jcp-2023-209190

Pierre-Marie Lavrut, Olivier Guillaud, Jérôme Dumortier, Elisabeth Mintz, Virginie Brun, Sophie Heissat, Eduardo Couchonnal Bedoya, Alain Lachaux, Muriel Bost, Valerie Hervieu

{"title":"Histological features of liver disease development in the Atp7b-/- mouse: a model of Wilson's disease.","authors":"Pierre-Marie Lavrut, Olivier Guillaud, Jérôme Dumortier, Elisabeth Mintz, Virginie Brun, Sophie Heissat, Eduardo Couchonnal Bedoya, Alain Lachaux, Muriel Bost, Valerie Hervieu","doi":"10.1136/jcp-2023-209190","DOIUrl":"10.1136/jcp-2023-209190","url":null,"abstract":"Aims: Wilson's disease (WD) is caused by mutations in the ATP7B gene, resulting in copper accumulation and toxicity in liver and brain tissues. Due to the initial asymptomatic liver involvement, the progression of liver injuries in WD stays primarily unknown. Atp7b-/- knockout mice have been shown to be an appropriate model of WD for liver involvement.Methods: A total of 138 Atp7b-/- mice were included and separated into five groups according to age as follows: 6, 20, 39 and 50 weeks without treatment, and 50 weeks with copper chelator treatment from 39 to 50 weeks of age and compared with 101 wild-type (WT) mice at the same stages. The evolution of histological liver lesions was analysed and compared between groups.Results: Significant changes were observed in Atp7b-/- mice compared with WT. Copper deposits in hepatocytes appeared as early as 6 weeks but no significant increase over time was observed. Inflammation appeared as early as 6 weeks and progressed henceforth. Lobular and periportal acidophilic bodies appeared after 20 weeks. Significant atypia was also observed at 20 weeks and increased over time to reach a severe stage at 39 weeks. Fibrosis also became apparent at 20 weeks, progressing subsequently to precirrhotic stages at 50 weeks. Copper content, inflammation and fibrosis scores were significantly reduced in the treated group. No bile duct lesions or dysplastic changes were noted.Conclusions: Copper accumulation leads to progressive changes in Atp7b-/- mice regarding inflammation, fibrosis and atypia. The severity of liver damage is lessened by chelation therapy.","PeriodicalId":15391,"journal":{"name":"Journal of Clinical Pathology","volume":" ","pages":"51-56"},"PeriodicalIF":2.5,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134649000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0