LGR5作为锯齿状息肉发育不良的诊断标志物。

IF 2.5 4区 医学 Q2 PATHOLOGY
Osman Yilmaz, Kshtij Arora, Soo Hyun Lee, Sahar Hosseini, Feidi Chen, Nandan Padmanabha, George Eng, Kanchan Kantekure, Omer Yilmaz, Vikram Deshpande
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引用次数: 0

摘要

目的:WNT信号通路失调通常是结直肠肿瘤的关键早期组成部分,尤其是染色体不稳定通路。使用两个WNT报告基因LGR5和AXIN2,我们试图评估这些息肉是否表现出可预测的表达模式,以及这些模式是否具有诊断价值。方法:对23例腺瘤(TA)、23例无梗锯齿状病变(SSLs)、14例SSL伴发育不良和38例传统锯齿状腺瘤(TSA)进行评估。对LGR5和AXIN2进行显色原位杂交染色(ISH)。反应性分为强、中、弱三种。上三隐窝反应性定义为全层染色。记录异位隐窝(ECF)内加重。结果:TAs(91%)反应性强,LGR5全层染色。TSA显示全层和弱至中等LGR5反应性(79%),LGR5加重的ECF仅见于TSA。SSL显示弱LGR5反应性局限于基底隐窝区(100%)。伴有发育不良的SSL也表现为弱或中等(100%)LGR5反应性,但反应性模式为全层(88%)。在检查的息肉组中,AXIN2的表达与LGR5的表达相似(Pearson系数=0.63)。结论:AXIN2和LGR5表达的定性和定量差异有助于SSL伴不典型增生的诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
LGR5 as a diagnostic marker for dysplasia in serrated polyps.

Aims: WNT signalling pathway dysregulation is often a critical early component in colorectal neoplasia, particularly the chromosomal instability pathway. Using two WNT reporters, LGR5 and AXIN2, we sought to assess whether these polyps demonstrate predictable expression patterns and if these patterns show diagnostic value.

Methods: We evaluated 23 adenomas (TA), 23 sessile serrated lesions (SSLs), 14 SSL with dysplasia and 38 traditional serrated adenomas (TSA). Chromogenic in situ hybridisation stains (ISH) for LGR5 and AXIN2 were performed. Reactivity was defined as strong, intermediate or weak. Upper third crypt reactivity was defined as full-thickness staining. Accentuation within ectopic crypts (ECF) was recorded.

Results: TAs (91%) showed strong reactivity and full-thickness staining with LGR5. TSAs showed full-thickness and weak to intermediate LGR5 reactivity (79%) and ECF with LGR5 accentuation was exclusively seen in TSA. SSL showed weak LGR5 reactivity confined to the basal crypt region (100%). SSL with dysplasia also showed weak or intermediate (100%) LGR5 reactivity, but the reactivity pattern was full thickness (88%). AXIN2 expression parallels LGR5 expression (Pearson coefficient=0.63) regarding signal intensity for the examined polyp groups.

Conclusions: Qualitative and quantitative differences in AXIN2 and LGR5 expression assist in the diagnosis of SSL with dysplasia.

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来源期刊
CiteScore
7.80
自引率
2.90%
发文量
113
审稿时长
3-8 weeks
期刊介绍: Journal of Clinical Pathology is a leading international journal covering all aspects of pathology. Diagnostic and research areas covered include histopathology, virology, haematology, microbiology, cytopathology, chemical pathology, molecular pathology, forensic pathology, dermatopathology, neuropathology and immunopathology. Each issue contains Reviews, Original articles, Short reports, Correspondence and more.
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