Journal of Chemical Biology最新文献_第8页

Design, synthesis, and evaluations of antifungal activity of novel phenyl(2H-tetrazol-5-yl)methanamine derivatives. 新型苯基(2h -四唑-5-基)甲烷胺衍生物的设计、合成和抗真菌活性评价。

Journal of Chemical Biology Pub Date : 2013-09-12 DOI: 10.1007/s12154-013-0103-8

Amol B Salake, Aparna S Chothe, Shrikant S Nilewar, Madhavi Khilare, Rutuja S Meshram, Abhishek A Pandey, M K Kathiravan

{"title":"Design, synthesis, and evaluations of antifungal activity of novel phenyl(2H-tetrazol-5-yl)methanamine derivatives.","authors":"Amol B Salake, Aparna S Chothe, Shrikant S Nilewar, Madhavi Khilare, Rutuja S Meshram, Abhishek A Pandey, M K Kathiravan","doi":"10.1007/s12154-013-0103-8","DOIUrl":"https://doi.org/10.1007/s12154-013-0103-8","url":null,"abstract":"Fungal infections pose a continuous and serious threat to human health and life. The intrinsic resistance has been observed in many genera of fungi. Many fungal infections are caused by opportunistic pathogens that may be endogenous (Candida infections) or acquired from the environment (Cryptococcus and Aspergillus infections). So, new therapeutic strategies are needed to combat various fungal infections. Fluconazole shows good antifungal activity with relatively low toxicity and is preferred as first line antifungal therapy, but it has suffered from severe drug resistance. So, there is a need to design novel analogues by modification of fluconazole-like structure. A novel series of phenyl(2H-tetrazol-5-yl)methanamine derivatives were synthesized by reaction of α-amino nitrile with sodium azide and ZnCl2 in presence of isopropyl alcohol. They were evaluated for antifungal activity against Candida albicans and Aspergillus niger and subjected to docking study against 1EA1. ","PeriodicalId":15296,"journal":{"name":"Journal of Chemical Biology","volume":"7 1","pages":"29-35"},"PeriodicalIF":0.0,"publicationDate":"2013-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12154-013-0103-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32035570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Enzyme immobilization: an update. 酶固定化:最新进展。

Journal of Chemical Biology Pub Date : 2013-08-29 DOI: 10.1007/s12154-013-0102-9

Ahmad Abolpour Homaei, Reyhaneh Sariri, Fabio Vianello, Roberto Stevanato

引用次数: 660

Alternative synthetic tools to phospho-specific antibodies for phosphoproteome analysis: progress and prospects. 用于磷蛋白组分析的替代合成工具:进展与展望。

Journal of Chemical Biology Pub Date : 2013-07-09 DOI: 10.1007/s12154-013-0100-y

James I Murray, Alan C Spivey, Rudiger Woscholski

{"title":"Alternative synthetic tools to phospho-specific antibodies for phosphoproteome analysis: progress and prospects.","authors":"James I Murray, Alan C Spivey, Rudiger Woscholski","doi":"10.1007/s12154-013-0100-y","DOIUrl":"https://doi.org/10.1007/s12154-013-0100-y","url":null,"abstract":"Signal transduction cascades in living systems are often controlled via post-translational phosphorylation and dephosphorylation of proteins. These processes are catalyzed in vivo by kinase and phosphatase enzymes, which consequently play an important role in many disease states, including cancer and immune system disorders. Current techniques for studying the phosphoproteome (isotopic labeling, chromatographic techniques, and phosphospecific antibodies), although undoubtedly very powerful, have yet to provide a generic tool for phosphoproteomic analysis despite the widespread utility such a technique would have. The use of small molecule organic catalysts that can promote selective phosphate esterification could provide a useful alternative to current state-of-the-art techniques for use in, e.g., the labeling and pull-down of phosphorylated proteins. This report reviews current techniques used for phosphoproteomic analysis and the recent use of small molecule peptide-based catalysts in phosphorylation reactions, indicating possible future applications for this type of catalyst as synthetic alternatives to phosphospecific antibodies for phosphoproteome analysis. ","PeriodicalId":15296,"journal":{"name":"Journal of Chemical Biology","volume":"6 4","pages":"175-84"},"PeriodicalIF":0.0,"publicationDate":"2013-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12154-013-0100-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32035567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

JOCB Bulletin. JOCB公告。

Journal of Chemical Biology Pub Date : 2013-06-19 DOI: 10.1007/s12154-013-0098-1

引用次数: 0

Fluorescence nanoscopy. Methods and applications. 荧光nanoscopy。方法和应用。

Journal of Chemical Biology Pub Date : 2013-06-04 DOI: 10.1007/s12154-013-0096-3

Jose Requejo-Isidro

引用次数: 32

Molecular recognition with boronic acids-applications in chemical biology. 硼酸的分子识别--在化学生物学中的应用。

Journal of Chemical Biology Pub Date : 2013-06-01 DOI: 10.1007/s12154-013-0099-0

Gillian F Whyte, Ramon Vilar, Rudiger Woscholski

引用次数: 0

Design and evaluation of new chemotherapeutics of aloe-emodin (AE) against the deadly cancer disease: an in silico study. 设计和评价芦荟大黄素(AE)治疗致命癌症的新化疗药物:一项计算机研究。

Journal of Chemical Biology Pub Date : 2013-05-23 DOI: 10.1007/s12154-013-0097-2

Chaitanya Mulakayala, Babajan Banaganapalli, Naveen Mulakayala, Madhusudana Pulaganti, Anuradha C M, Suresh Kumar Chitta

引用次数: 6

Development of rapid microwave-mediated and low-temperature bacterial transformations. 快速微波介导和低温细菌转化的发展。

Journal of Chemical Biology Pub Date : 2013-05-21 DOI: 10.1007/s12154-013-0095-4

Valerie T Tripp, Johnathan C Maza, Douglas D Young

引用次数: 7

Fluorescent labeling and modification of proteins. 蛋白质的荧光标记和修饰。

Journal of Chemical Biology Pub Date : 2013-04-13 DOI: 10.1007/s12154-013-0094-5

Christopher P Toseland

引用次数: 0

The lipidome and proteome of oil bodies from Helianthus annuus (common sunflower). 向日葵(Helianthus annuus)油体脂质组和蛋白质组。

Journal of Chemical Biology Pub Date : 2013-04-01 Epub Date: 2013-01-26 DOI: 10.1007/s12154-012-0090-1

Samuel Furse, Susan Liddell, Catharine A Ortori, Huw Williams, D Cameron Neylon, David J Scott, David A Barrett, David A Gray

{"title":"The lipidome and proteome of oil bodies from Helianthus annuus (common sunflower).","authors":"Samuel Furse, Susan Liddell, Catharine A Ortori, Huw Williams, D Cameron Neylon, David J Scott, David A Barrett, David A Gray","doi":"10.1007/s12154-012-0090-1","DOIUrl":"https://doi.org/10.1007/s12154-012-0090-1","url":null,"abstract":"In this paper we report the molecular profiling, lipidome and proteome, of the plant organelle known as an oil body (OB). The OB is remarkable in that it is able to perform its biological role (storage of triglycerides) whilst resisting the physical stresses caused by changes during desiccation (dehydration) and germination (rehydration). The molecular profile that confers such extraordinary physical stability on OBs was determined using a combination of (31)P/(1)H nuclear magnetic resonance (NMR), high-resolution mass spectrometry and nominal mass-tandem mass spectrometry for the lipidome, and gel-electrophoresis-chromatography-tandem mass spectrometry for the proteome. The integrity of the procedure for isolating OBs was supported by physical evidence from small-angle neutron-scattering experiments. Suppression of lipase activity was crucial in determining the lipidome. There is conclusive evidence that the latter is dominated by phosphatidylcholine (∼60 %) and phosphatidylinositol (∼20 %), with a variety of other head groups (∼20 %). The fatty acid profile of the surface monolayer comprised palmitic, linoleic and oleic acids (2:1:0.25, (1)H NMR) with only traces of other fatty acids (C24:0, C22:0, C18:0, C18:3, C16:2; by MS). The proteome is rich in oleosins (78 %) with the remainder being made up of caleosins and steroleosins. These data are sufficiently detailed to inform an update of the understood model of this organelle and can be used to inform the use of such components in a range of molecular biological, biotechnological and food industry applications. The techniques used in this study for profiling the lipidome throw a new light on the lipid profile of plant cellular compartments.","PeriodicalId":15296,"journal":{"name":"Journal of Chemical Biology","volume":" ","pages":"63-76"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12154-012-0090-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40228370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 42