{"title":"荧光nanoscopy。方法和应用。","authors":"Jose Requejo-Isidro","doi":"10.1007/s12154-013-0096-3","DOIUrl":null,"url":null,"abstract":"<p><p>Fluorescence nanoscopy refers to the experimental techniques and analytical methods used for fluorescence imaging at a resolution higher than conventional, diffraction-limited, microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses on the latest and promising developments in acquisition techniques, labelling strategies to obtain highly detailed super-resolved images and in the quantitative methods to extract meaningful information from them. </p>","PeriodicalId":15296,"journal":{"name":"Journal of Chemical Biology","volume":"6 3","pages":"97-120"},"PeriodicalIF":0.0000,"publicationDate":"2013-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12154-013-0096-3","citationCount":"32","resultStr":"{\"title\":\"Fluorescence nanoscopy. Methods and applications.\",\"authors\":\"Jose Requejo-Isidro\",\"doi\":\"10.1007/s12154-013-0096-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Fluorescence nanoscopy refers to the experimental techniques and analytical methods used for fluorescence imaging at a resolution higher than conventional, diffraction-limited, microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses on the latest and promising developments in acquisition techniques, labelling strategies to obtain highly detailed super-resolved images and in the quantitative methods to extract meaningful information from them. </p>\",\"PeriodicalId\":15296,\"journal\":{\"name\":\"Journal of Chemical Biology\",\"volume\":\"6 3\",\"pages\":\"97-120\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-06-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/s12154-013-0096-3\",\"citationCount\":\"32\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Chemical Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/s12154-013-0096-3\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chemical Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s12154-013-0096-3","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Fluorescence nanoscopy refers to the experimental techniques and analytical methods used for fluorescence imaging at a resolution higher than conventional, diffraction-limited, microscopy. This review explains the concepts behind fluorescence nanoscopy and focuses on the latest and promising developments in acquisition techniques, labelling strategies to obtain highly detailed super-resolved images and in the quantitative methods to extract meaningful information from them.
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