Journal of Chemical Biology最新文献_第7页

Synthesis and investigation of new Hesperadin analogues antitumor effects on HeLa cells. 新型橙皮苷类似物的合成及对HeLa细胞抗肿瘤作用的研究。

Journal of Chemical Biology Pub Date : 2014-05-18 eCollection Date: 2014-07-01 DOI: 10.1007/s12154-014-0111-3

Fereshteh Shamsipour, Saeeideh Hosseinzadeh, Seyed Shahriar Arab, Sedigheh Vafaei, Samira Farid, Mahmood Jeddi-Tehrani, Saeed Balalaie

引用次数: 4

Towards predictive resistance models for agrochemicals by combining chemical and protein similarity via proteochemometric modelling. 通过蛋白质化学计量学建模，结合化学和蛋白质相似性，建立农用化学品的预测抗性模型。

Journal of Chemical Biology Pub Date : 2014-05-15 eCollection Date: 2014-10-01 DOI: 10.1007/s12154-014-0112-2

Gerard J P van Westen, Andreas Bender, John P Overington

{"title":"Towards predictive resistance models for agrochemicals by combining chemical and protein similarity via proteochemometric modelling.","authors":"Gerard J P van Westen, Andreas Bender, John P Overington","doi":"10.1007/s12154-014-0112-2","DOIUrl":"https://doi.org/10.1007/s12154-014-0112-2","url":null,"abstract":"Resistance to pesticides is an increasing problem in agriculture. Despite practices such as phased use and cycling of 'orthogonally resistant' agents, resistance remains a major risk to national and global food security. To combat this problem, there is a need for both new approaches for pesticide design, as well as for novel chemical entities themselves. As summarized in this opinion article, a technique termed 'proteochemometric modelling' (PCM), from the field of chemoinformatics, could aid in the quantification and prediction of resistance that acts via point mutations in the target proteins of an agent. The technique combines information from both the chemical and biological domain to generate bioactivity models across large numbers of ligands as well as protein targets. PCM has previously been validated in prospective, experimental work in the medicinal chemistry area, and it draws on the growing amount of bioactivity information available in the public domain. Here, two potential applications of proteochemometric modelling to agrochemical data are described, based on previously published examples from the medicinal chemistry literature. ","PeriodicalId":15296,"journal":{"name":"Journal of Chemical Biology","volume":"7 4","pages":"119-23"},"PeriodicalIF":0.0,"publicationDate":"2014-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12154-014-0112-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32752048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Increased lipid droplet accumulation associated with a peripheral sensory neuropathy. 脂滴积聚增加与周围感觉神经病变有关。

Journal of Chemical Biology Pub Date : 2014-03-23 eCollection Date: 2014-04-01 DOI: 10.1007/s12154-014-0108-y

Lee L Marshall, Scott E Stimpson, Ryan Hyland, Jens R Coorssen, Simon J Myers

{"title":"Increased lipid droplet accumulation associated with a peripheral sensory neuropathy.","authors":"Lee L Marshall, Scott E Stimpson, Ryan Hyland, Jens R Coorssen, Simon J Myers","doi":"10.1007/s12154-014-0108-y","DOIUrl":"https://doi.org/10.1007/s12154-014-0108-y","url":null,"abstract":"Hereditary sensory neuropathy type 1 (HSN-1) is an autosomal dominant neurodegenerative disease caused by missense mutations in the SPTLC1 gene. The SPTLC1 protein is part of the SPT enzyme which is a ubiquitously expressed, critical and thus highly regulated endoplasmic reticulum bound membrane enzyme that maintains sphingolipid concentrations and thus contributes to lipid metabolism, signalling, and membrane structural functions. Lipid droplets are dynamic organelles containing sphingolipids and membrane bound proteins surrounding a core of neutral lipids, and thus mediate the intracellular transport of these specific molecules. Current literature suggests that there are increased numbers of lipid droplets and alterations of lipid metabolism in a variety of other autosomal dominant neurodegenerative diseases, including Alzheimer's and Parkinson's disease. This study establishes for the first time, a significant increase in the presence of lipid droplets in HSN-1 patient-derived lymphoblasts, indicating a potential connection between lipid droplets and the pathomechanism of HSN-1. However, the expression of adipophilin (ADFP), which has been implicated in the regulation of lipid metabolism, was not altered in lipid droplets from the HSN-1 patient-derived lymphoblasts. This appears to be the first report of increased lipid body accumulation in a peripheral neuropathy, suggesting a fundamental molecular linkage between a number of neurodegenerative diseases. ","PeriodicalId":15296,"journal":{"name":"Journal of Chemical Biology","volume":"7 2","pages":"67-76"},"PeriodicalIF":0.0,"publicationDate":"2014-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12154-014-0108-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32246990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Gold nanoparticle modifies nitric oxide release and vasodilation in rat aorta. 金纳米颗粒修饰大鼠主动脉一氧化氮释放和血管舒张。

Journal of Chemical Biology Pub Date : 2014-03-23 eCollection Date: 2014-04-01 DOI: 10.1007/s12154-014-0109-x

Bruno R Silva, Claure N Lunardi, Koiti Araki, Juliana C Biazzotto, Roberto S Da Silva, Lusiane M Bendhack

{"title":"Gold nanoparticle modifies nitric oxide release and vasodilation in rat aorta.","authors":"Bruno R Silva, Claure N Lunardi, Koiti Araki, Juliana C Biazzotto, Roberto S Da Silva, Lusiane M Bendhack","doi":"10.1007/s12154-014-0109-x","DOIUrl":"https://doi.org/10.1007/s12154-014-0109-x","url":null,"abstract":"Nitric oxide (NO) plays an important role on several biological functions. Recently, it has been reported the possibility of modifying the NO release profile from the NO donors through its coupling to gold nanoparticles (AuNPs). Thus, AuNPs were synthesized and they were exposed to the NO donor ruthenium complex Cis-[Ru(bpy)2(NO)(4PySH)].(PF6)3 termed (Ru-4PySH)-forming AuNPs-{Ru-4PySH}n cluster. Our results indicate that AuNPs do not modify the maximum effect (ME) and potency (pD2) in the vasodilation induced by Ru-4PySH. Both complexes induce similar vascular relaxation in concentration-dependent way. However, the NO released from the complex AuNPs-{Ru-4PySH}n is lower than Ru-4PySH. Both complexes release only NO(0) specie, but AuNPs-{Ru-4PySH}n releases NO in constant way and exclusively in the extracellular medium. In time-course, Ru-4Py-SH was faster than AuNPs-{Ru-4PySH}n in inducing the maximum vasodilation. Inhibition of soluble guanylyl cyclase (sGC) abolished the vasodilation induced by Ru-4PYSH, but not by AuNPs-{Ru-4PySH}n. Non-selective potassium (K(+)) channel blocker TEA had no effect on the vasodilation induced by AuNPs-{Ru-4PySH}n, but it reduced the potency to Ru-4PySH. In conclusion, our results suggest that AuNPs can reduce the permeability of NO donor Ru-4PySH due to AuNPs-{Ru-4PySH}n cluster formation. AuNPs reduce NO release, but they do not impair the vasodilator effect induced by the NO donor. Ru-4PySH induces vasodilation by sGC and K(+) channels activation, while AuNPs-{Ru-4PySH}n activates mainly sGC. Taken together, these findings represent a new pharmacological strategy to control the NO release which could activate selective biological targets. ","PeriodicalId":15296,"journal":{"name":"Journal of Chemical Biology","volume":"7 2","pages":"57-65"},"PeriodicalIF":0.0,"publicationDate":"2014-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12154-014-0109-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32246989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

JOCB Bulletin

Journal of Chemical Biology Pub Date : 2014-01-01 DOI: 10.1007/s12154-014-0110-4

引用次数: 0

JOCB Bulletin. JOCB公告。

Journal of Chemical Biology Pub Date : 2013-12-11 DOI: 10.1007/s12154-013-0107-4

引用次数: 0

Transient conformational remodeling of folding proteins by GroES-individually and in concert with GroEL. 由GroEL单独和与GroEL合作的折叠蛋白的瞬时构象重塑。

Journal of Chemical Biology Pub Date : 2013-10-05 eCollection Date: 2013-01-01 DOI: 10.1007/s12154-013-0106-5

Satish Babu Moparthi, Daniel Sjölander, Laila Villebeck, Bengt-Harald Jonsson, Per Hammarström, Uno Carlsson

引用次数: 8

Quantification of exocytosis kinetics by DIC image analysis of cortical lawns. 皮质草坪胞吐动力学的DIC图像定量分析。

Journal of Chemical Biology Pub Date : 2013-09-27 eCollection Date: 2014-04-01 DOI: 10.1007/s12154-013-0104-7

James Mooney, Saumitra Thakur, Peter Kahng, Josef G Trapani, Dominic Poccia

{"title":"Quantification of exocytosis kinetics by DIC image analysis of cortical lawns.","authors":"James Mooney, Saumitra Thakur, Peter Kahng, Josef G Trapani, Dominic Poccia","doi":"10.1007/s12154-013-0104-7","DOIUrl":"https://doi.org/10.1007/s12154-013-0104-7","url":null,"abstract":"Cortical lawns prepared from sea urchin eggs have offered a robust in vitro system for study of regulated exocytosis and membrane fusion events since their introduction by Vacquier almost 40 years ago (Vacquier in Dev Biol 43:62-74, 1975). Lawns have been imaged by phase contrast, darkfield, differential interference contrast, and electron microscopy. Quantification of exocytosis kinetics has been achieved primarily by light scattering assays. We present simple differential interference contrast image analysis procedures for quantifying the kinetics and extent of exocytosis in cortical lawns using an open vessel that allows rapid solvent equilibration and modification. These preparations maintain the architecture of the original cortices, allow for cytological and immunocytochemical analyses, and permit quantification of variation within and between lawns. When combined, these methods can shed light on factors controlling the rate of secretion in a spatially relevant cellular context. We additionally provide a subroutine for IGOR Pro® that converts raw data from line scans of cortical lawns into kinetic profiles of exocytosis. Rapid image acquisition reveals spatial variations in time of initiation of individual granule fusion events with the plasma membrane not previously reported. ","PeriodicalId":15296,"journal":{"name":"Journal of Chemical Biology","volume":"7 2","pages":"43-55"},"PeriodicalIF":0.0,"publicationDate":"2013-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12154-013-0104-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32246988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

JOCB Bulletin. JOCB公告。

Journal of Chemical Biology Pub Date : 2013-09-27 DOI: 10.1007/s12154-013-0105-6

引用次数: 0

Towards 3D in silico modeling of the sea urchin embryonic development. 海胆胚胎发育的三维计算机模拟。

Journal of Chemical Biology Pub Date : 2013-09-13 DOI: 10.1007/s12154-013-0101-x

Barbara Rizzi, Nadine Peyrieras

{"title":"Towards 3D in silico modeling of the sea urchin embryonic development.","authors":"Barbara Rizzi, Nadine Peyrieras","doi":"10.1007/s12154-013-0101-x","DOIUrl":"10.1007/s12154-013-0101-x","url":null,"abstract":"Embryogenesis is a dynamic process with an intrinsic variability whose understanding requires the integration of molecular, genetic, and cellular dynamics. Biological circuits function over time at the level of single cells and require a precise analysis of the topology, temporality, and probability of events. Integrative developmental biology is currently looking for the appropriate strategies to capture the intrinsic properties of biological systems. The \"-omic\" approaches require disruption of the function of the biological circuit; they provide static information, with low temporal resolution and usually with population averaging that masks fast or variable features at the cellular scale and in a single individual. This data should be correlated with cell behavior as cells are the integrators of biological activity. Cellular dynamics are captured by the in vivo microscopy observation of live organisms. This can be used to reconstruct the 3D + time cell lineage tree to serve as the basis for modeling the organism's multiscale dynamics. We discuss here the progress that has been made in this direction, starting with the reconstruction over time of three-dimensional digital embryos from in toto time-lapse imaging. Digital specimens provide the means for a quantitative description of the development of model organisms that can be stored, shared, and compared. They open the way to in silico experimentation and to a more theoretical approach to biological processes. We show, with some unpublished results, how the proposed methodology can be applied to sea urchin species that have been model organisms in the field of classical embryology and modern developmental biology for over a century. ","PeriodicalId":15296,"journal":{"name":"Journal of Chemical Biology","volume":"7 1","pages":"17-28"},"PeriodicalIF":0.0,"publicationDate":"2013-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s12154-013-0101-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31997123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10