Journal of Biomedical Optics最新文献

{"title":"Optical coherence tomography-enabled classification of the human venoatrial junction.","authors":"Arielle S Joasil, Aidan M Therien, Christine P Hendon","doi":"10.1117/1.JBO.30.1.016005","DOIUrl":"10.1117/1.JBO.30.1.016005","url":null,"abstract":"<p><strong>Significance: </strong>Radiofrequency ablation to treat atrial fibrillation (AF) involves isolating the pulmonary vein from the left atria to prevent AF from occurring. However, creating ablation lesions within the pulmonary veins can cause adverse complications.</p><p><strong>Aim: </strong>We propose automated classification algorithms to classify optical coherence tomography (OCT) volumes of human venoatrial junctions.</p><p><strong>Approach: </strong>A dataset of comprehensive OCT volumes of 26 venoatrial junctions was used for this study. Texture, statistical, and optical features were extracted from OCT patches. Patches were classified as a left atrium or pulmonary vein using random forest (RF), logistic regression (LR), and convolutional neural networks (CNNs). The features were inputs into the RF and LR classifiers. The inputs to the CNNs included: (1) patches and (2) an ensemble of patches and patch-derived features.</p><p><strong>Results: </strong>Utilizing a sevenfold cross-validation, the patch-only CNN balances sensitivity and specificity best, with an area under the receiver operating characteristic (AUROC) curve of <math><mrow><mn>0.84</mn> <mo>±</mo> <mn>0.109</mn></mrow> </math> across the test sets. RF is more sensitive than LR, with an AUROC curve of <math><mrow><mn>0.78</mn> <mo>±</mo> <mn>0.102</mn></mrow> </math> .</p><p><strong>Conclusions: </strong>Cardiac tissues can be identified in benchtop OCT images by automated analysis. Extending this analysis to data obtained <i>in vivo</i> is required to tune automated analysis further. Performing this classification <i>in vivo</i> could aid doctors in identifying substrates of interest and treating AF.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 1","pages":"016005"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11747903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of signal-to-noise ratio and contrast definition on the sensitivity assessment and benchmarking of fluorescence molecular imaging systems.","authors":"Elena Kriukova, Ethan LaRochelle, T Joshua Pfefer, Udayakumar Kanniyappan, Sylvain Gioux, Brian Pogue, Vasilis Ntziachristos, Dimitris Gorpas","doi":"10.1117/1.JBO.30.S1.S13703","DOIUrl":"10.1117/1.JBO.30.S1.S13703","url":null,"abstract":"<p><strong>Significance: </strong>Standardization of fluorescence molecular imaging (FMI) is critical for ensuring quality control in guiding surgical procedures. To accurately evaluate system performance, two metrics, the signal-to-noise ratio (SNR) and contrast, are widely employed. However, there is currently no consensus on how these metrics can be computed.</p><p><strong>Aim: </strong>We aim to examine the impact of SNR and contrast definitions on the performance assessment of FMI systems.</p><p><strong>Approach: </strong>We quantified the SNR and contrast of six near-infrared FMI systems by imaging a multi-parametric phantom. Based on approaches commonly used in the literature, we quantified seven SNRs and four contrast values considering different background regions and/or formulas. Then, we calculated benchmarking (BM) scores and respective rank values for each system.</p><p><strong>Results: </strong>We show that the performance assessment of an FMI system changes depending on the background locations and the applied quantification method. For a single system, the different metrics can vary up to <math><mrow><mo>∼</mo> <mn>35</mn> <mtext>  </mtext> <mi>dB</mi></mrow> </math> (SNR), <math><mrow><mo>∼</mo> <mn>8.65</mn> <mtext>  </mtext> <mi>a</mi> <mo>.</mo> <mi>u</mi></mrow> </math> . (contrast), and <math><mrow><mo>∼</mo> <mn>0.67</mn> <mtext>  </mtext> <mi>a</mi> <mo>.</mo> <mi>u</mi></mrow> </math> . (BM score).</p><p><strong>Conclusions: </strong>The definition of precise guidelines for FMI performance assessment is imperative to ensure successful clinical translation of the technology. Such guidelines can also enable quality control for the already clinically approved indocyanine green-based fluorescence image-guided surgery.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 Suppl 1","pages":"S13703"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11256003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of cold storage on double integrating sphere optical property measurements of porcine dermis and subcutaneous fat from 400 to 1100 nm.","authors":"Maria A T Hoffman, Mark A Keppler, Andrea L Smith, Anjelyka Fasci, Matthew E Macasadia, Amanda J Tijerina, Robert Lyle Hood, Michael P DeLisi, Joel N Bixler","doi":"10.1117/1.JBO.30.1.015001","DOIUrl":"10.1117/1.JBO.30.1.015001","url":null,"abstract":"<p><strong>Significance: </strong>Accurate values of skin optical properties are essential for developing reliable computational models and optimizing optical imaging systems. However, published values show a large variability due to a variety of factors, including differences in sample collection, preparation, experimental methodology, and analysis.</p><p><strong>Aim: </strong>We aim to explore the influence of storage conditions on the optical properties of the excised skin from 400 to 1100 nm.</p><p><strong>Approach: </strong>We utilize a double integrating sphere system and inverse adding-doubling approach to determine absorption, <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> , and reduced scattering, <math> <mrow> <msubsup><mrow><mi>μ</mi></mrow> <mrow><mi>s</mi></mrow> <mrow><mo>'</mo></mrow> </msubsup> </mrow> </math> , coefficients of the porcine dermis and subcutaneous fat before and after refrigeration, freezing, or flash freezing.</p><p><strong>Results: </strong>Our findings indicate a small average change of <math><mrow><mo>-</mo> <mn>0.005</mn></mrow> </math> , <math><mrow><mo>-</mo> <mn>0.003</mn></mrow> </math> , and <math><mrow><mn>0.002</mn> <mtext>  </mtext> <msup><mrow><mi>mm</mi></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </mrow> </math> in <math> <mrow><msub><mi>μ</mi> <mi>a</mi></msub> </mrow> </math> for the dermis and 0.001, <math><mrow><mo>-</mo> <mn>0.003</mn></mrow> </math> , and <math><mrow><mo>-</mo> <mn>0.008</mn> <mtext>  </mtext> <msup><mrow><mi>mm</mi></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </mrow> </math> for the subcutaneous tissue after refrigeration, freezing, and flash freezing, respectively, with the most notable differences observed in the hemoglobin absorption region. The value of <math> <mrow><msubsup><mi>μ</mi> <mi>s</mi> <mo>'</mo></msubsup> </mrow> </math> shows a negligible average change of <math><mrow><mo>-</mo> <mn>0.05</mn></mrow> </math> , <math><mrow><mo>-</mo> <mn>0.001</mn></mrow> </math> , and <math><mrow><mo>-</mo> <mn>0.02</mn> <mo> </mo> <msup><mi>mm</mi> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </mrow> </math> for the dermis, and 0.06, <math><mrow><mo>-</mo> <mn>0.1</mn></mrow> </math> , and <math><mrow><mn>0.03</mn> <mtext>  </mtext> <msup><mrow><mi>mm</mi></mrow> <mrow><mo>-</mo> <mn>1</mn></mrow> </msup> </mrow> </math> change for the subcutaneous tissue for refrigerated, frozen, and flash-frozen samples, respectively.</p><p><strong>Conclusions: </strong>The results provide additional context for the variability of published values of optical parameters and enable informed selection of sample storage conditions for future measurements. In addition, the results discussed here can be used to improve study planning, particularly with regard to maximizing the use of finite samples that have been collected.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 1","pages":"015001"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751729/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Speckle-illumination spatial frequency domain imaging with a stereo laparoscope for profile-corrected optical property mapping.","authors":"Anthony A Song, Mason T Chen, Taylor L Bobrow, Nicholas J Durr","doi":"10.1117/1.JBO.30.S1.S13710","DOIUrl":"10.1117/1.JBO.30.S1.S13710","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Significance: &lt;/strong&gt;Laparoscopic surgery presents challenges in localizing oncological margins due to poor contrast between healthy and malignant tissues. Optical properties can uniquely identify various tissue types and disease states with high sensitivity and specificity, making it a promising tool for surgical guidance. Although spatial frequency domain imaging (SFDI) effectively measures quantitative optical properties, its deployment in laparoscopy is challenging due to the constrained imaging environment. Thus, there is a need for compact structured illumination techniques to enable accurate, quantitative endogenous contrast in minimally invasive surgery.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim: &lt;/strong&gt;We introduce a compact, two-camera laparoscope that incorporates both active stereo depth estimation and speckle-illumination SFDI (si-SFDI) to map profile-corrected, pixel-level absorption ( &lt;math&gt; &lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;μ&lt;/mi&gt; &lt;mi&gt;a&lt;/mi&gt;&lt;/msub&gt; &lt;/mrow&gt; &lt;/math&gt; ), and reduced scattering ( &lt;math&gt; &lt;mrow&gt; &lt;msubsup&gt;&lt;mrow&gt;&lt;mi&gt;μ&lt;/mi&gt;&lt;/mrow&gt; &lt;mrow&gt;&lt;mi&gt;s&lt;/mi&gt;&lt;/mrow&gt; &lt;mrow&gt;&lt;mo&gt;'&lt;/mo&gt;&lt;/mrow&gt; &lt;/msubsup&gt; &lt;/mrow&gt; &lt;/math&gt; ) optical properties in images of tissues with complex geometries.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Approach: &lt;/strong&gt;We used a multimode fiber-coupled 639-nm laser illumination to generate high-contrast speckle patterns on the object. These patterns were imaged through a modified commercial stereo laparoscope for optical property estimation via si-SFDI. Compared with the original si-SFDI work, which required &lt;math&gt;&lt;mrow&gt;&lt;mo&gt;≥&lt;/mo&gt; &lt;mn&gt;10&lt;/mn&gt;&lt;/mrow&gt; &lt;/math&gt; images of randomized speckle patterns for accurate optical property estimations, our approach approximates the DC response using a laser speckle reducer (LSR) and consequently requires only two images. In addition, we demonstrate 3D profilometry using active stereo from low-coherence RGB laser flood illumination. Sample topography was then used to correct for measured intensity variations caused by object height and surface angle differences with respect to a calibration phantom. The low-contrast RGB speckle pattern was blurred using an LSR to approximate incoherent white light illumination. We validated profile-corrected si-SFDI against conventional SFDI in phantoms with simple and complex geometries, as well as in a human finger &lt;i&gt;in vivo&lt;/i&gt; time-series constriction study.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Laparoscopic si-SFDI optical property measurements agreed with conventional SFDI measurements when measuring flat tissue phantoms, exhibiting an error of 6.4% for absorption and 5.8% for reduced scattering. Profile-correction improved the accuracy for measurements of phantoms with complex geometries, particularly for absorption, where it reduced the error by 23.7%. An &lt;i&gt;in vivo&lt;/i&gt; finger constriction study further validated laparoscopic si-SFDI, demonstrating an error of 8.2% for absorption and 5.8% for reduced scattering compared with conventional SFDI. Moreover, the observed trends in optical properties du","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 Suppl 1","pages":"S13710"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Year, New JBO Editor-in-Chief.","authors":"Muyinatu A Lediju Bell","doi":"10.1117/1.JBO.30.1.010101","DOIUrl":"https://doi.org/10.1117/1.JBO.30.1.010101","url":null,"abstract":"<p><p>JBO Editor in Chief Muyinatu Bell highlights gratitude and forthcoming initiatives for the new year.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 1","pages":"010101"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of intraoperative thermal and optical imaging for identification of the human primary sensory cortex.","authors":"Juliane Müller, Martin Oelschlägel, Stephan B Sobottka, Matthias Kirsch, Gerald Steiner, Edmund Koch, Christian Schnabel","doi":"10.1117/1.JBO.30.1.016002","DOIUrl":"10.1117/1.JBO.30.1.016002","url":null,"abstract":"<p><strong>Significance: </strong>The precise identification and preservation of functional brain areas during neurosurgery are crucial for optimizing surgical outcomes and minimizing postoperative deficits. Intraoperative imaging plays a vital role in this context, offering insights that guide surgeons in protecting critical cortical regions.</p><p><strong>Aim: </strong>We aim to evaluate and compare the efficacy of intraoperative thermal imaging (ITI) and intraoperative optical imaging (IOI) in detecting the primary somatosensory cortex, providing a detailed assessment of their potential integration into surgical practice.</p><p><strong>Approach: </strong>Data from nine patients undergoing tumor resection in the region of the somatosensory cortex were analyzed. Both IOI and ITI were employed simultaneously, with a specific focus on the areas identified as the primary somatosensory cortex (S1 region). The methodologies included a combination of imaging techniques during distinct phases of rest and stimulation, confirmed by electrophysiological monitoring of somatosensory evoked potentials to verify the functional areas identified by both imaging methods. The data were analyzed using a Fourier-based analytical framework to distinguish physiological signals from background noise.</p><p><strong>Results: </strong>Both ITI and IOI successfully generated reliable activity maps following median nerve stimulation. IOI showed greater consistency across various clinical scenarios, including those involving cortical tumors. Quantitative analysis revealed that IOI could more effectively differentiate genuine neuronal activity from artifacts compared with ITI, which was occasionally prone to false positives in the presence of cortical abnormalities.</p><p><strong>Conclusions: </strong>ITI and IOI produce comparable functional maps with moderate agreement in Cohen's kappa values. Their distinct physiological mechanisms suggest complementary use in specific clinical scenarios, such as cortical tumors or impaired neurovascular coupling. IOI excels in spatial resolution and mapping reliability, whereas ITI provides additional insights into metabolic changes and tissue properties, especially in pathological areas. Combined, these modalities could enhance the understanding and analysis of functional and pathological processes in complex neurosurgical cases.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 1","pages":"016002"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection properties of indium-111 and IRDye800CW for intraoperative molecular imaging use across tissue phantom models.","authors":"ReidAnn E Sever, Lauren T Rosenblum, Kayla C Stanley, Angel G Cortez, Dominic M Menendez, Bhuvitha Chagantipati, Jessie R Nedrow, W Barry Edwards, Marcus M Malek, Gary Kohanbash","doi":"10.1117/1.JBO.30.S1.S13705","DOIUrl":"10.1117/1.JBO.30.S1.S13705","url":null,"abstract":"<p><strong>Significance: </strong>Intraoperative molecular imaging (IMI) enables the detection and visualization of cancer tissue using targeted radioactive or fluorescent tracers. While IMI research has rapidly expanded, including the recent Food and Drug Administration approval of a targeted fluorophore, the limits of detection have not been well-defined.</p><p><strong>Aim: </strong>The ability of widely available handheld intraoperative tools (Neoprobe and SPY-PHI) to measure gamma decay and fluorescence intensity from IMI tracers was assessed while varying characteristics of both the signal source and the intervening tissue or gelatin phantoms.</p><p><strong>Approach: </strong>Gamma decay signal and fluorescence from tracer-bearing tumors (TBTs) and modifiable tumor-like inclusions (TLIs) were measured through increasing thicknesses of porcine tissue and gelatin in custom 3D-printed molds. TBTs buried beneath porcine tissue were used to simulate IMI-guided tumor resection.</p><p><strong>Results: </strong>Gamma decay from TBTs and TLIs was detected through significantly thicker tissue and gelatin than fluorescence, with at least 5% of the maximum signal observed through up to 5 and 0.5 cm, respectively, depending on the overlying tissue type or gelatin.</p><p><strong>Conclusions: </strong>We developed novel systems that can be fine-tuned to simulate variable tumor characteristics and tissue environments. These were used to evaluate the detection of fluorescent and gamma signals from IMI tracers and simulate IMI surgery.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 Suppl 1","pages":"S13705"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11413652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic comparison of fluorescence imaging in the near-infrared and shortwave-infrared spectral range using clinical tumor samples containing cetuximab-IRDye800CW.","authors":"Bas Keizers, Thomas S Nijboer, Christa A M van der Fels, Marius C van den Heuvel, Gooitzen M van Dam, Schelto Kruijff, Igle Jan de Jong, Max J H Witjes, Floris J Voskuil, Dimitris Gorpas, Wesley R Browne, Pieter J van der Zaag","doi":"10.1117/1.JBO.30.S1.S13708","DOIUrl":"10.1117/1.JBO.30.S1.S13708","url":null,"abstract":"<p><strong>Significance: </strong>Shortwave-infrared (SWIR) imaging is reported to yield better contrast in fluorescence-guided surgery than near-infrared (NIR) imaging, due to a reduction in scattering. This benefit of SWIR was shown in animal studies, however not yet in clinical studies with patient samples.</p><p><strong>Aim: </strong>We investigate the potential benefit of SWIR to NIR imaging in clinical samples containing cetuximab-IRDye800CW in fluorescence-guided surgery.</p><p><strong>Approach: </strong>The potential of the epidermal growth factor-targeted NIR dye cetuximab-IRDye800CW in the shortwave range was examined by recording the absorption and emission spectrum. An <i>ex vivo</i> comparison of NIR and SWIR images using clinical tumor samples of patients with penile squamous cell carcinoma (PSCC) and head and neck squamous cell carcinoma (HNSCC) containing cetuximab-IRDye800CW was performed. The comparison was based on the tumor-to-background ratio and an adapted contrast-to-noise ratio (aCNR) using the standard of care pathology tissue assessment as the golden standard.</p><p><strong>Results: </strong>Based on the emission spectrum, cetuximab-IRDye800CW can be detected in the SWIR range. In clinical PSCC samples, overall SWIR imaging was found to perform similarly to NIR imaging (NIR imaging is better than SWIR in the 2/7 criteria examined, and SWIR is better than NIR in the 3/7 criteria). However, when inspecting HNSCC data, NIR is better than SWIR in nearly all (5/7) examined criteria. This difference seems to originate from background autofluorescence overwhelming the off-peak SWIR fluorescence signal in HNSCC tissue.</p><p><strong>Conclusion: </strong>SWIR imaging using the targeted tracer cetuximab-IRDye800CW currently does not provide additional benefit over NIR imaging in <i>ex vivo</i> clinical samples. Background fluorescence in the SWIR region, resulting in a higher background signal, limits SWIR imaging in HNSCC samples. However, SWIR shows potential in increasing the contrast of tumor borders in PSCC samples, as shown by a higher aCNR over a line.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 Suppl 1","pages":"S13708"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11566260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-color fluorescence-guided surgery for head and neck cancer resections.","authors":"Dani A Szafran, Nourhan A Shams, Antonio Montaño, Syed Zaki Husain Rizvi, Adam W G Alani, Kimberley S Samkoe, Lei G Wang, Summer L Gibbs","doi":"10.1117/1.JBO.30.S1.S13707","DOIUrl":"https://doi.org/10.1117/1.JBO.30.S1.S13707","url":null,"abstract":"<p><strong>Significance: </strong>Head and neck squamous cell carcinoma (HNSCC) has the sixth highest incidence worldwide, with <math><mrow><mo>></mo> <mn>650,000</mn></mrow> </math> cases annually. Surgery is the primary treatment option for HNSCC, during which surgeons balance two main goals: (1) complete cancer resection and (2) preservation of normal tissues to ensure post-surgical quality of life. Unfortunately, these goals are not synergistic, where complete cancer resection is often limited by efforts to preserve normal tissues, particularly nerves, and reduce life-altering comorbidities.</p><p><strong>Aim: </strong>Currently, no clinically validated technology exists to enhance intraoperative cancer and nerve recognition. Fluorescence-guided surgery (FGS) has successfully integrated into clinical medicine, providing surgeons with real-time visualization of important tissues and complex anatomy, where FGS imaging systems operate almost exclusively in the near-infrared (NIR, 650 to 900 nm). Notably, this spectral range permits the detection of two NIR imaging channels for spectrally distinct detection.</p><p><strong>Approach: </strong>Herein, we evaluated the utility of spectrally distinct NIR nerve- and tumor-specific fluorophores for two-color FGS to guide HNSCC surgery. Using a human HNSCC xenograft murine model, we demonstrated that facial nerves and tumors could be readily differentiated using these nerve- and tumor-specific NIR fluorophores.</p><p><strong>Results: </strong>The selected nerve-specific fluorophore showed no significant difference in nerve specificity and off-target tissue fluorescence in the presence of xenograft head and neck tumors. Co-administration of two NIR fluorophores demonstrated successful tissue-specific labeling of nerves and tumors in spectrally distinct NIR imaging channels.</p><p><strong>Conclusions: </strong>We demonstrate a comprehensive FGS tool for cancer resection and nerve sparing during HNSCC procedures for future clinical translation.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"30 Suppl 1","pages":"S13707"},"PeriodicalIF":3.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum: Publisher's Note: Optical clearing with tartrazine enables deep transscleral imaging with optical coherence tomography.","authors":"Amit Narawane, Robert Trout, Christian Viehland, Anthony N Kuo, Lejla Vajzovic, Al-Hafeez Dhalla, Cynthia A Toth","doi":"10.1117/1.JBO.29.12.129801","DOIUrl":"https://doi.org/10.1117/1.JBO.29.12.129801","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1117/1.JBO.29.12.120501.].</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 12","pages":"129801"},"PeriodicalIF":3.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}