Journal of biomedical nanotechnology最新文献_第5页

Folate Receptor-Targeted Nanodelivery of Apigenin in Breast Cancer: Formulation Development, Characterization and In Vitro Evaluation 叶酸受体靶向纳米递送芹菜素治疗乳腺癌：配方开发、表征和体外评估

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-04-01 DOI: 10.1166/jbn.2024.3803

Arjun Patra, Swaha Satpathy, Pradeep K. Naik, Mohsin Kazi, Muhammad Hussain Delwar

{"title":"Folate Receptor-Targeted Nanodelivery of Apigenin in Breast Cancer: Formulation Development, Characterization and In Vitro Evaluation","authors":"Arjun Patra, Swaha Satpathy, Pradeep K. Naik, Mohsin Kazi, Muhammad Hussain Delwar","doi":"10.1166/jbn.2024.3803","DOIUrl":"https://doi.org/10.1166/jbn.2024.3803","url":null,"abstract":"Cancer is a dreadful disease with a high mortality rate and breast cancer is the most common cancer among females in the world. Different strategies have been used for the treatment of breast cancer, including chemotherapy but it has a wide range of side effects. This problem can be\u0000 overcome by delivering anticancer agents with nano-formulations. Apigenin (4′,5,7-trihydroxyflavone), present in many different medicinal plants, shows potential anticancer properties in various cancers. However, its use in clinical practice is limited due to its low water solubility\u0000 and bioavailability. In this study, we examined folate receptor-targeted and PEGylated poly(lactide-co-glycolide) nanoparticles (PLGA-PEG-FA NPs) containing apigenin for targeted delivery to MCF-7 breast cancer cells. Apigenin-loaded PLGA-PEG and PLGA-PEG-FA NPs were small in size, had a negative\u0000 zeta potential, showed sustained release of apigenin and showed significantly higher anticancer activity than the free drug in breast cancer cells. The half maximal inhibitory concentration (IC50) values of apigenin, apigenin-loaded PLGA, PLGA-PEG and PLGA-PEG-FA NPs were 50.2,\u0000 49.4, 18.1 and 13.3 μM, respectively. Apigenin-loaded PLGA-PEG and PLGA-PEG-FA NPs showed 2.79- and 3.77-fold higher cytotoxicity than the pristine drug. Folate-conjugated PLGA nanoparticles could be developed for potential target-specific delivery of apigenin in the treatment of\u0000 breast cancer.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140355789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Piezo1 Channel-Mediated Mechanotransduction on Osteogenic Differentiation and Interleukin-6 Secretion in Bone Mesenchymal Stem Cells Under Tensile Strain Piezo1 通道介导的机械传导对拉伸应变下骨间质干细胞成骨分化和白细胞介素-6 分泌的影响

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-04-01 DOI: 10.1166/jbn.2024.3730

Xu Yan, Su Fu, Ying Xie, Xuejian Wu

{"title":"Effect of Piezo1 Channel-Mediated Mechanotransduction on Osteogenic Differentiation and Interleukin-6 Secretion in Bone Mesenchymal Stem Cells Under Tensile Strain","authors":"Xu Yan, Su Fu, Ying Xie, Xuejian Wu","doi":"10.1166/jbn.2024.3730","DOIUrl":"https://doi.org/10.1166/jbn.2024.3730","url":null,"abstract":"Physical stimulation plays a crucial role in the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (MSCs). However, the mechanotransductive mechanisms remain uncleared. Recent studies have suggested that the Piezo1 channel is essential for transforming mechanical\u0000 signals. Therefore, we investigate the Piezo1-mediated mechanisms in mechanical strain-regulated MSC osteogenic differentiation and release of proinflammatory cytokines. The tensile strain was applied to rat MSCs cultured in a monolayer to induce mechanical strain. The immuno-nanomagnetic\u0000 bead enzyme-linked immunosorbent assay was employed to assess gene and protein expressions, as well as osteogenic biomarkers and interleukin-6 (IL-6) release, both in the presence or absence of a Piezo1 agonist/antagonist. Firstly, biophysical loading through mechanical strain was found to\u0000 promote MSC osteogenic differentiation. Suppression of Piezo1 using GsMTx4 antagonist or transfection with Piezo1-siRNA effectively inhibited mechanical responses associated with osteogenic gene expressions and IL-6. Activation of Piezo1 by Yoda1 mimicked the effects induced by mechanical\u0000 strain on osteogenic gene expressions and IL-6 release, which were associated with YAP activation, upregulation, and nuclear accumulation of β-catenin. In conclusion, these findings significantly enhance our understanding of MSC mechanotransduction and hold great promise for drug\u0000 development to enhance skeletal mass.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140356041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation, Characterization, and Bioactivities of Cobalt, Strontium and Fluorine Co-Doped Oxide Films on Titanium Surface for Clinical Application 用于临床应用的钛表面钴、锶和氟共掺氧化物薄膜的制备、表征和生物活性

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-04-01 DOI: 10.1166/jbn.2024.3813

Xinglin Wu, Yang Jiao, Jieshi Wu, Sujiajun Zhang, Ruisheng Xu, Quanming Zhao, K. Lu, Pengpeng Zhang, Lu Zhang, Xiaohui Ni

{"title":"Preparation, Characterization, and Bioactivities of Cobalt, Strontium and Fluorine Co-Doped Oxide Films on Titanium Surface for Clinical Application","authors":"Xinglin Wu, Yang Jiao, Jieshi Wu, Sujiajun Zhang, Ruisheng Xu, Quanming Zhao, K. Lu, Pengpeng Zhang, Lu Zhang, Xiaohui Ni","doi":"10.1166/jbn.2024.3813","DOIUrl":"https://doi.org/10.1166/jbn.2024.3813","url":null,"abstract":"Titanium and titanium alloys are receiving widespread attention due to their excellent comprehensive mechanical properties, corrosion resistances, and biocompatibilities. However, titanium metal itself is biologically inert in physiological environments, and after implantation, it is\u0000 surrounded by a layer of encapsulating fibrous membrane, making it difficult to form solid bonds with the tissue. Plasma electrolytic oxidation is a new technology used to prepare bioactive porous ceramic membranes on the surfaces of titanium and titanium alloys. It has application prospects\u0000 for biomimetic surface modifications of titanium alloys. In this study, a cobalt, strontium and fluorine codoped oxide film (TAM-CSF) was prepared on a titanium surface via plasma electrolytic oxidation. The surface characteristics of the film were evaluated with field emission scanning electron\u0000 microscopy, energy spectrum analyses, atomic force microscopy, profilometry and X-ray photoelectron spectroscopy. Additionally, the corrosion performance of the material was evaluated with an electrochemical workstation. The biocompatibility and bioactivity of the film were tested with in\u0000 vitro cell experiments. The results showed that the TAM-CSF on the titanium surface had a porous morphology, and the CSF was uniformly doped on the film surface. TAM-CSF improved the surface roughness of the titanium. This film exhibited good biocompatibility and promoted the extension\u0000 and proliferation of MC3T3-E1 cells. It was possible to prepare TAM-CSF on titanium surfaces via plasma electrolytic oxidation. The film exhibited a good morphology and good biological activity and has clinical application prospects.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140356965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-Noncoding RNA (lncRNA) EGOT Prevents the Malignant Process of Colorectal Carcinoma by Regulating BTG3 长非编码 RNA（lncRNA）EGOT 通过调控 BTG3 防止结直肠癌的恶性过程

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-04-01 DOI: 10.1166/jbn.2024.3823

Zhengdong Wang, Dezhi Gu, Aiming Zhou

{"title":"Long-Noncoding RNA (lncRNA) EGOT Prevents the Malignant Process of Colorectal Carcinoma by Regulating BTG3","authors":"Zhengdong Wang, Dezhi Gu, Aiming Zhou","doi":"10.1166/jbn.2024.3823","DOIUrl":"https://doi.org/10.1166/jbn.2024.3823","url":null,"abstract":"This study investigates the role of the long non-coding RNA EGOT in colorectal cancer (CRC) by examining its expression in 40 pairs of CRC and adjacent normal tissues and assessing its impact on clinical outcomes. EGOT was found to be downregulated in CRC tissues, and low EGOT levels\u0000 were associated with a higher likelihood of lymphatic and distant metastasis, as well as poorer overall and progression-free survival in CRC patients. Functional experiments revealed that overexpression of EGOT in SW480 cells reduced cell viability, migration, and wound closure, while knockdown\u0000 of EGOT in LoVo cells had the opposite effect. In vivo experiments with nude mice confirmed that EGOT downregulation accelerated CRC growth, whereas its overexpression slowed tumor growth. The study identified BTG3 as the target gene of EGOT, and they exhibited a negative correlation\u0000 in CRC tissues. Rescue experiments demonstrated that BTG3 could reverse the effects of EGOT on CRC cell phenotypes. In conclusion, EGOT is a downregulated molecule in CRC, closely associated with metastasis and patient prognosis. It exerts a suppressive influence on CRC cell proliferation,\u0000 migration, and tumorigenesis by negatively regulating BTG3.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140352737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Verification of Potential Ferroptosis-Related Biomarkers in Cervical Cancer 宫颈癌中潜在的铁蛋白沉积相关生物标记物的鉴定与验证

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-04-01 DOI: 10.1166/jbn.2024.3810

Zhaodi Liu, Yang Liu, Xinyue Wu, Xiangnan Feng, Wei Liang

{"title":"Identification and Verification of Potential Ferroptosis-Related Biomarkers in Cervical Cancer","authors":"Zhaodi Liu, Yang Liu, Xinyue Wu, Xiangnan Feng, Wei Liang","doi":"10.1166/jbn.2024.3810","DOIUrl":"https://doi.org/10.1166/jbn.2024.3810","url":null,"abstract":"This study screened important genes contributing to morbidity from differential ferroptosis-related genes (FRGs) in cervical cancer and to establish a risk assessment model with ferroptosis-related LncRNAs. Total RNA sequencing data were extracted from The cancer genome atlas (TCGA),\u0000 Gene Expression Omnibus (GEO) and Genotype-Tissue Expression (GTEx). By differential analysis, we identified ferroptosis-related hub genes close to prevalence of cervical cancer. According to receiver operator curves (ROC) curves, hub genes have good diagnostic performance. The diagnostic\u0000 potential of hub genes for occurrence of the disease was further assessed and verified. Further, a risk-assessing model based on ferroptosis-related LncRNAs was established. Finally, the differential expressions of hub genes were verified through qRT-PCR. Five hub genes were identified, and\u0000 we found through GO, KEGG and immune infiltration, that the hub genes are connection with cervical cancer. The Area Under Curve (AUC) values were all greater than 0.8 in ROC curve, and the hub genes presented differences between disease and control groups in validation set GSE29570. We created\u0000 a risk assessment model with 16 ferroptosis-related LncRNAs. There was a difference in survival between high-risk and low-risk groups. The AUC result for risk assessment model reached 0.792, and there were significant expression differences of Hub genes in Huvec and Hela cells. The study screened\u0000 5 hub genes and constructed the risk-assessment model based on 16 LncRNAs associated with ferroptosis genes.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140355903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Associations of Inflammation, Oxidative Stress and Prognosis with IL-37 Expression in Sepsis Rats with Lung Injury 脓毒症肺损伤大鼠的炎症、氧化应激和预后与 IL-37 表达的关系

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-04-01 DOI: 10.1166/jbn.2024.3822

Fei Hong, Jungang Zhao, Mei Yang, Qian Liu, Qin Chen, Feng Liu, Guoji Zhu

{"title":"Associations of Inflammation, Oxidative Stress and Prognosis with IL-37 Expression in Sepsis Rats with Lung Injury","authors":"Fei Hong, Jungang Zhao, Mei Yang, Qian Liu, Qin Chen, Feng Liu, Guoji Zhu","doi":"10.1166/jbn.2024.3822","DOIUrl":"https://doi.org/10.1166/jbn.2024.3822","url":null,"abstract":"This study aimed to investigate the role of interleukin-37 (IL-37) expression in lung tissues of sepsis-induced acute lung injury (ALI) rats and its impact on ALI, along with the underlying mechanisms. Sprague-Dawley (SD) rats were categorized into three groups: Control, sepsis-induced\u0000 ALI (via cecal ligation and puncture, CLP), and sepsis-induced ALI with antibiotics (CLP+An). ALI models were established, and lung tissue injuries were assessed through hematoxylineosin staining. mRNA levels of IL-1α, IL-1β, IL-37, and tumor necrosis factor-α\u0000 (TNF-α) were measured via RT-PCR, while IL-37 protein levels in lung tissues were determined using Western blotting. Additionally, bronchoalveolar lavage fluid (BALF) and blood samples were collected to assess inflammatory factors through ELISA. In the CLP group, there was an\u0000 increase in pro-inflammatory factors (IL-1α, IL-1β, and TNF-α) in lung tissues and serum. However, in the CLP+An group, these factors decreased, IL-37 expression increased, and oxidative stress levels decreased. IL-37 demonstrated an inhibitory effect\u0000 on the release of pro-inflammatory factors (IL-1α, IL-1β, and TNF-α) in sepsis rats, leading to a reduction in lung tissue inflammation. Furthermore, IL-37 exhibited a protective role by reducing oxidative stress in sepsis-induced lung tissues. These findings\u0000 highlight IL-37 as a potential therapeutic target for mitigating ALI in sepsis.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140356666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-92a Promotes Apoptosis in Rats with Myocardial Ischemia-Reperfusion Injury via Regulating Wnt/β-Catenin Pathway MiR-92a 通过调节 Wnt/β-Catenin 通路促进心肌缺血再灌注损伤大鼠的细胞凋亡

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-04-01 DOI: 10.1166/jbn.2024.3818

Yong Ye, Guang Xia, Min Chen, Jifu Jin, Linxiang Lu, Xin Wang

{"title":"MiR-92a Promotes Apoptosis in Rats with Myocardial Ischemia-Reperfusion Injury via Regulating Wnt/β-Catenin Pathway","authors":"Yong Ye, Guang Xia, Min Chen, Jifu Jin, Linxiang Lu, Xin Wang","doi":"10.1166/jbn.2024.3818","DOIUrl":"https://doi.org/10.1166/jbn.2024.3818","url":null,"abstract":"In this study, the impact of micro ribonucleic acid (miR)-92a on rats with myocardial ischemia-reperfusion injury was investigated, with a focus on its regulation of the Wnt/β-catenin pathway. A total of 36 Sprague Dawley rats were divided into three groups: a sham operation\u0000 group, a model group, and a miR-92a antagomir group. The sham group underwent thoracotomy without injury, while the model and miR-92a antagomir groups were subjected to myocardial ischemiareperfusion injury and treated with saline and miR-92a antagomir, respectively. Results showed that the\u0000 myocardial infarction area was significantly reduced in the miR-92a antagomir group compared to the model group. Histological analysis revealed improved myocardial tissue structure in the miR-92a antagomir group. Western blotting demonstrated elevated levels of p-GSK-3β and β-catenin\u0000 in both the model and miR-92a antagomir groups, with a notable decrease in the miR-92a antagomir group compared to the model group. Additionally, miR-92a expression was higher in both the model and miR-92a antagomir groups compared to the sham group. Lastly, apoptosis rates were increased\u0000 in both the model and miR-92a antagomir groups, but significantly reduced in the miR-92a antagomir group compared to the model group. Overall, these findings suggest that miR-92a exacerbates apoptosis in rats with myocardial ischemia-reperfusion injury by up-regulating the Wnt/β-catenin\u0000 signaling pathway.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140354169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Radiotherapy on Radiation-Induced Brain Injury in Patients with Nasopharyngeal Carcinoma Using Nano-Magnetic Resonance Imaging 利用纳米磁共振成像分析放疗对鼻咽癌患者放射性脑损伤的影响

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-04-01 DOI: 10.1166/jbn.2024.3809

Baomin Zheng, Hanyong Zhang, Weihu Wang

{"title":"Impact of Radiotherapy on Radiation-Induced Brain Injury in Patients with Nasopharyngeal Carcinoma Using Nano-Magnetic Resonance Imaging","authors":"Baomin Zheng, Hanyong Zhang, Weihu Wang","doi":"10.1166/jbn.2024.3809","DOIUrl":"https://doi.org/10.1166/jbn.2024.3809","url":null,"abstract":"This study investigate the changes of white matter injury induced by radiation after radiotherapy in patients with nasopharyngeal carcinoma (NPC) and its association with cognitive dysfunction using multiple MRI methods. A total of 42 patients with NPC who underwent radiotherapy at\u0000 xxx Hospital between December 2018 and June 2021 were included. The patients were randomly divided into 4 groups based on the timing of radiotherapy. Superparamagnetic iron oxide nanoparticles were used as MRI contrast agents. DTI and MRS scans were conducted to measure FA, ADC, NAA/Cho, Cho/Cr,\u0000 and NAA/Cr ratios in the hippocampus of both temporal lobes. A cognitive assessment was performed using the MoCA and MMSE scales. After radiotherapy, patients experienced a decline in cognitive scores, which stabilized after 6 months. White matter changes were observed in the hippocampus,\u0000 with decreased FA and increased ADC values that gradually returned to normal levels. Cho value increased and NAA value decreased initially but eventually returned to pre-treatment levels. No significant changes occurred in the Cr value. Metabolite ratios decreased within 3 months post-radiotherapy\u0000 but gradually increased thereafter, remaining lower than pre-treatment levels at 6 months. Higher radiation doses did not significantly affect FA and ADC values but decreased white matter metabolite ratios. In conclusion, we reveal that the dosage and duration of radiotherapy can influence\u0000 the degree of brain injury in patients with NPC and highlights the cognitive decline, white matter changes, and changes in metabolite ratios after radiotherapy for NPC, providing insights into the effects of radiation on brain structure and function.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140357298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of Bone Homeostasis Imbalance in Osteoporotic Fractures by Mesoporous Silica Carrying miR-302b 携带 miR-302b 的介孔二氧化硅可改善骨质疏松性骨折的骨平衡失调状况

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-04-01 DOI: 10.1166/jbn.2024.3808

Jiaqi Chu, Yuan Si, Song Shao

{"title":"Improvement of Bone Homeostasis Imbalance in Osteoporotic Fractures by Mesoporous Silica Carrying miR-302b","authors":"Jiaqi Chu, Yuan Si, Song Shao","doi":"10.1166/jbn.2024.3808","DOIUrl":"https://doi.org/10.1166/jbn.2024.3808","url":null,"abstract":"miR-302b and DKK1 are two molecules related to the regulation of bone metabolism. Mesoporous silica is a potential drug carrier. This article aims to study the mechanism of mesoporous silica carrying miR-302b targeting DKK1 regulation to improve bone homeostasis imbalance in osteoporotic\u0000 fractures. Mesoporous silica nanoparticles were synthesized and characterized. miR-302b was loaded into mesoporous silica to form composite nanoparticles. In vivo rat model experiments were performed to evaluate bone metabolism. X-ray examination and μCT scan were used to\u0000 detect the bone content and trabecular bone status of rats. Alcian blue/hematoxylin/Orange G staining was used to observe changes in trabecular bone in the tibial metaphysis. Immunohistochemical staining showed the formation of trabecular bone in rats in each group and changes in the number\u0000 of bone cells. Calcein double labeling experiment showed the bone mineralization speed of mice in each group. Pure and stable mesoporous silica nanoparticles were successfully synthesized and miR-302b was successfully loaded into the nanoparticles. The osteoporotic fracture rat model was successfully\u0000 created. In vivo experimental results showed that after injecting composite nanoparticles into mice, bone density and bone strength were significantly increased and osteoporotic fractures were improved. Mesoporous silica carries miR-302b to target DKK1 regulation, which can improve\u0000 bone homeostasis imbalance in osteoporotic fractures. Composite nanoparticles can inhibit the expression of DKK1, promote bone formation, and inhibit bone resorption, thereby improving bone density and bone strength.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140353673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treatment Promotion of Osteoporotic Fractures by microRNA-320 Nanocapsules Through Stimulating Bone Marrow Mesenchymal Stem Cells microRNA-320 纳米胶囊通过刺激骨髓间充质干细胞促进骨质疏松性骨折的治疗

IF 2.9 4区医学

Journal of biomedical nanotechnology Pub Date : 2024-03-01 DOI: 10.1166/jbn.2024.3784

Ligang Qian, Qinggui Li, Qiao Ren

引用次数: 0