Ring Finger Protein 38 Induces Colorectal Cancer Proliferation by Activating the Phosphatidyl- Inositol 3-Kinase/Serine-Threonine Kinase Signaling

IF 2.9 4区医学 Q1 Medicine

Journal of biomedical nanotechnology Pub Date : 2023-11-01 DOI:10.1166/jbn.2023.3698

Junde Zhou, Meng Qiao, Haiyan Zhao, Nannan Lu, Xinxin Lv, Xin Wang, Jing Li, Lixia Ke

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Abstract

Previous studies have identified that ring finger protein 38 (RNF38) induces proliferative potential in many types of tumors. However, its functions in colorectal cancer (CRC) are unclear. This study aims to elucidate the regulatory effects of RNF38 on CRC proliferation in vitro and in vivo. Expression pattern and prognostic potential of RNF38 in clinically collected CRC cases were analyzed. After intervening RNF38 levels in SW480 and HT29 cells, viability, cell cycle progression and apoptosis were examined. Subsequently, we generated RNF38 overexpressed transgenic mice and xenograft model in nude mice. in vivo regulation of RNF38 on CRC proliferation was assessed. RNF38 was upregulated in CRC tissues. Overexpression of RNF38 stimulated proliferative ability and inhibited apoptosis in CRC cells. In addition, in vivo overexpression of RNF38 triggered tumor growth of CRC in nude mice. Silence of RNF38 markedly suppressed in vivo proliferation of CRC and induced apoptosis by activating the PI3K/AKT signaling.

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环指蛋白 38 通过激活磷脂酰肌醇-3-激酶/丝氨酸-苏氨酸激酶信号传导诱导结直肠癌增殖

以往的研究发现，环指蛋白 38（RNF38）可诱导多种类型肿瘤的增殖潜能。然而，它在结直肠癌（CRC）中的功能尚不清楚。本研究旨在阐明 RNF38 在体外和体内对 CRC 增殖的调控作用。研究分析了 RNF38 在临床收集的 CRC 病例中的表达模式和预后潜力。在对 SW480 和 HT29 细胞中的 RNF38 水平进行干预后，对细胞活力、细胞周期进展和细胞凋亡进行了检测。随后，我们制作了过表达 RNF38 的转基因小鼠和裸鼠异种移植模型，评估了 RNF38 对 CRC 增殖的体内调控作用。RNF38在CRC组织中上调。过表达 RNF38 可刺激 CRC 细胞的增殖能力并抑制其凋亡。此外，在裸鼠体内过表达 RNF38 会诱发 CRC 肿瘤生长。通过激活PI3K/AKT信号转导，沉默RNF38可明显抑制CRC的体内增殖并诱导细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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