Journal of Bioanalysis & Biomedicine最新文献

{"title":"GAG Nanoparticles for Brain Repair and Beyond: A Commentary on Nih et al., Nature Materials 2018","authors":"S. Anderson","doi":"10.4172/1948-593X.1000212","DOIUrl":"https://doi.org/10.4172/1948-593X.1000212","url":null,"abstract":"In the May 21, 2018 issue of Nature Materials, Nih et al. artfully revealed how heparin nanoparticles can be employed to regrow brain tissue after damage from stroke [1]. This landmark finding revolutionizes the way we think of stroke and recovery, and also demonstrates the unrivaled power of tissue engineering. Further, if heparin nanoparticles can down-regulate the natural inflammatory response and stimulate morphological repair of complex tissue structures in the brain, strong hope exists that the heparin nanoparticles can also repair damaged heart tissue, diabetes-induced necrotic tissue, chronic wounds, and spinal cord injuries.","PeriodicalId":15259,"journal":{"name":"Journal of Bioanalysis & Biomedicine","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80550471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Medicines Cold Chain Storage Conformity with the National Requirements in Governmental Health Care Facilities of Nekemte Town, Western Ethiopia","authors":"Ginenus Fekadu, G. Merga, M. Gebre","doi":"10.4172/1948-593x.1000219","DOIUrl":"https://doi.org/10.4172/1948-593x.1000219","url":null,"abstract":"Cold chain is the system of transporting and storing vaccines within the safe temperature range of 2°C to 8°C. The chain begins with the refrigerator or freezer at the vaccine manufacturing plant, vaccine distributor and then to the provider’s office (immunization clinic) and ends with the administration of the vaccine to the recipients [1]. Health facilities in developing countries are supposed to maintain the cold chain to ensure that temperature sensitive medicines reach consumers in good quality [2,3].","PeriodicalId":15259,"journal":{"name":"Journal of Bioanalysis & Biomedicine","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87293498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional Treatment of Anaemia as Compare to Marketed Available Drug (Ferrous Sulfate) in Animal Model","authors":"K. Baqa, N. Waris, R. Kausar, Anum Butt, Iqra Saleem, N. Sultana","doi":"10.4172/1948-593X.1000204","DOIUrl":"https://doi.org/10.4172/1948-593X.1000204","url":null,"abstract":"Aim: To study the nutritional treatment of anaemia as compare to marketed available drug (Ferrous sulfate) in animal model.Methodology: Total 24 rats were included in the study, divided into 4 groups. Control group was treated with saline. Anaemia was produced in model and both test groups by pricking on their tail and drawn 2-3 drops of blood daily for one week. After producing anaemia model remained same and test group 1 were treated with the marketed available anti-anaemic drug called Ferrous sulphate of 30 mg/kg body weight and test group 2 treated with natural anti-anaemic Illicium verum extract of 80 mg/kg body weight for 6 weeks respectively.Result: Illicium verum extract increases locomotor activity in T2 rats therefore; rats open field activity was increased. It decreases depression and long-term memory but enhanced short term memory. It also produces anxiolytic effect as compare to ferrous sulfate. Haematological study including RBC, PCV, MCHC and platelets were shown significantly increased in T2 group of rats while lymphocyte, MCH and MCV were decreased.Conclusion: It was concluded in the study that the Illicium verum extract produce same effect as common medicine ferrous sulfate on rat’s model. In developing country, such as Pakistan the rate of anaemia was increased so there is a need of finding natural compounds to cure anaemia. Natural remedies were vital in modern era because natural compound do not produce side effect.","PeriodicalId":15259,"journal":{"name":"Journal of Bioanalysis & Biomedicine","volume":"1 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83086939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Magnesium Sulfate on Mitochondrial Oxygen Consumption Rate In vitro Study","authors":"A. A. Zaabi, A. Al-Rahmani","doi":"10.4172/1948-593X.1000206","DOIUrl":"https://doi.org/10.4172/1948-593X.1000206","url":null,"abstract":"Hypoxic insults initiate a cascade of biochemical events that result in irreversible neuronal damage. Magnesium sulfate agent has a possible neuroprotective effect as it can work at different stages of hypoxic brain injury. Objectives: Magnesium sulfate is used in the neonatal management of HIE as an adjunct medication to reduce Hypoxic Neuronal injury. In this Vitro study, we aimed to examine the efficacy of using magnesium sulfate in human cells to reduce oxygen consumption. We used in-vitro method utilizing foreskin as human cell surrogate. Method: Foreskin specimens from healthy newborns were collected immediately after circumcision and processed within 1 h for measuring the cellular rate of O2 consumption. Samples were placed in the oxygen phosphorescence analyzer and allowed to run for approximately 1 h. Injection then added magnesium sulfate at different doses into the vial. We analyze the O2 consumption in the samples at different levels of Magnesium Sulfate; the results were plotted using Kaleida Graph TM software. Results: The rate of respiration reduced with increasing the dose of MgSo4. The Cumulative analysis of cellular respiration rate was before and after an addition of MgSO4. Collectively yielding a (k) value of 0.08 μM O2/min μM O2 min-1 mg- and 0.04 M O2/min μM O2 min-1 mg- respectively with a significant P-value of <0.001. Conclusion: Magnesium sulfate reduces the rate of O2 Consumption in a dose-dependent manner.","PeriodicalId":15259,"journal":{"name":"Journal of Bioanalysis & Biomedicine","volume":"25 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82501327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nipah Viral Virulence may be Due to Resistant Strains Amplified by Cross- Species Transmission between Animals and Humans","authors":"S. Nandi","doi":"10.4172/1948-593X.1000213","DOIUrl":"https://doi.org/10.4172/1948-593X.1000213","url":null,"abstract":"Nipah virus is one of the ribonucleic acid viruses belong to the family members of Paramyxoviridae, genus Henipavirus. NiV was first originated from Sungai Nipah, a village in the Malaysian Peninsula where pig farmers became ill due to respiratory tract infection followed by encephalitis. NiV attacked pig farmers or abattoir workers which were first reported in September 1998 to April 1999 in Malaysia and then spread to neighbor Singapore by March 1999 [1,2] and in India and Bangladesh in 2001 [3]. Many 105 people died in Malaysia in April 1999, and one fellow was died in Singapore in the same year [1]. In 2001, a number of 45 persons were died followed by the death of 5 persons in 2007 in India whereas from 2001-2013 a number of 221 infected out of which 171 were being died in Bangladesh [4]. The morbidity and mortality statistics are given in Table 1.","PeriodicalId":15259,"journal":{"name":"Journal of Bioanalysis & Biomedicine","volume":"108 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73116787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pattern of Antibiotics Use for Prophylaxis and Treatment among Patients Who Undergone Major Surgery at Nekemte Referral Hospital, West Ethiopia","authors":"Tasissa Ayela Wokuma, Mohammed Gebre Dedefo","doi":"10.4172/1948-593X.1000217","DOIUrl":"https://doi.org/10.4172/1948-593X.1000217","url":null,"abstract":"","PeriodicalId":15259,"journal":{"name":"Journal of Bioanalysis & Biomedicine","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77972586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of USP18 in Insulin Resistance","authors":"Anjing Zhu, Limin Chen, Yujia Li","doi":"10.4172/1948-593X.1000E158","DOIUrl":"https://doi.org/10.4172/1948-593X.1000E158","url":null,"abstract":"Host innate immunity is regarded as the first line of defense to fight against virus infections. Type I Interferon (IFN) is one of the main components in the innate immune response [1]. Type I and III IFNs function through activating the Jak/STAT signaling pathway and then up-regulating the expression levels of hundreds of interferon stimulated genes(ISGs) [2,3]. Many ISGs, including ubiquitin-specific protease 18 (USP18, also known as UBP43), exert important antiviral and/or immuno-modulatory effects in different stages of virus infection [4].","PeriodicalId":15259,"journal":{"name":"Journal of Bioanalysis & Biomedicine","volume":"148 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75522473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the Effects of Oxidative Stress on Some Reproductive Hormones in Male Hypertensive Subjects at NAUTH, Nnewi","authors":"E. Onwubuya, N. Ukibe, O. A. Kalu, Bonaventure Sunday Agbo, Sn Ukibe, Ifeoma Nwamaka Monago, O. Emelumadu","doi":"10.4172/1948-593X.1000207","DOIUrl":"https://doi.org/10.4172/1948-593X.1000207","url":null,"abstract":"Background: This is a prospective case control study, aimed at assessing the effect of oxidative stress using Malondialdehyde (MDA) Superoxide Dismutase (SOD) and Total antioxidant capacity (TAC) on some reproductive hormones Follicle stimulating hormone (FSH), Luteinizing hormone (LH) and Testosterone (TT) in male hypertensive subjects at NAUTH Nnewi, Anambra State, Nigeria.Methods: 90 newly diagnosed adult male hypertensive and sixty normotensive control subjects aged between 30-65 (49.98 ± 9.90) years were randomly recruited for the study. Blood sample were collected from all the participants, separated and stored frozen at ˗20°C until assayed for male sex hormones using standard ELISA methods and oxidative stress markers (MDA, SOD and TAC) using Spectrophotometric method. Blood pressures were measured using accosson sphygmomanometer and stethoscope, and high blood pressure was considered when systolic blood pressure (SBP) is greater than 140 mmHg and diastolic blood pressure (DBP) greater than 90 mmHg persistently for three days. A well-structured questionnaire was administered to ascertain the bio-data and anthropometric index of all the participants.Results: SBP and DBP were significantly higher in hypertensive male subjects 157.10 ± 15.40, 99.40 ± 8.94) compared to controls (114.50 ± 8.3, 76.60 ± 5.90) (p=0.000 respectively). FSH and LH (11.94 ± 4.14, 8.46 ± 2.54) were significantly higher while TT (3.19 ± 1.63) was significantly lower in hypertensive subjects compared to controls (7.16 ± 3.40, 3.31 ± 1.74) (7.32 ± 2.54) (p=0.000 respectively). More so, the mean levels of MDA and SOD in hypertensive subjects (1.43 ± 0.45, 15.36 ± 6.15) were significantly higher compared to control (0.63 ± 0.29, 5.28 ± 2.70) (p=0.000) while TAC in hypertensive subjects (18.19 ± 5.46) was significantly lower compared to control subjects (36.93 ± 7.55) (p=0.000). Significant positive correlation was observed between SBP, DBP and FSH, LH, SOD, MDA while significant negative correlation existed between SBP, DBP and TT, TAC. TT and LH were significantly positively correlated with TAC and MDA.Conclusion: Hypertensive male subjects showed significantly higher levels of FSH and LH with lower testosterone levels which suggest hypogonadism and significantly higher MDA and SOD with lower levels of TAC indicating some degree of oxidative stress with suppressed body’s antioxidant defense. The positive and negative correlation observed in blood pressure, male sex hormones and oxidative stress markers also showed that oxidative stress may contribute significantly to the hypogonadism observed which may affect the reproductive potentials in these subjects.","PeriodicalId":15259,"journal":{"name":"Journal of Bioanalysis & Biomedicine","volume":"133 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86475715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D Printing of Bioactive Ceramic Scaffolds for Bone Tissue Engineering","authors":"Yufang Zhu","doi":"10.4172/1948-593X.1000E155","DOIUrl":"https://doi.org/10.4172/1948-593X.1000E155","url":null,"abstract":"Bone tissue engineering has emerged as an innovative and promising strategy for treating bone defects, in which a three-dimensional (3D) porous scaffold is loaded with tissue-inducing factors or specific cells to launch a tissue regeneration in a natural way [1,2]. A variety of materials, consisting of bimetallic materials, bioceramics, biopolymers or bio composites, have been proposed and used to fabricate scaffolds for bone tissue engineering over the last few decades, while the functions similar to natural bone remains a challenging task [3]. Architecture, mechanical property and osteogenic ability are considered as the most critical characteristics for an ideal scaffold [4]. Many bioceramic materials have high stiffness and bioactivity (i.e., their similarity to the mineral phase of natural bone) [5,6], which can act as a temporary framework for providing a suitable environment for cell adhesion, growth, and more explicitly help bone tissue regeneration. Bioceramics refer to a class of ceramic materials with specific biological or physiological function, and can be used directly in the human body or in applications related to the human body [7].","PeriodicalId":15259,"journal":{"name":"Journal of Bioanalysis & Biomedicine","volume":"220 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75172131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interdisciplinary Research on Consent of Alzheimer’s Patients to Medical Procedures","authors":"Ewa M Guzik Makaruk, Magda Olesiuk Okomska","doi":"10.4172/1948-593x.1000211","DOIUrl":"https://doi.org/10.4172/1948-593x.1000211","url":null,"abstract":"Mini Review The high-priority issues of today and pressing research questions require insights from multiple disciplines. In order to address the main challenges facing the global society, scientists of various fields have to combine their efforts and must work together. Nowadays, research goes well beyond conventional academic boundaries, whereas bringing scientists from a variety of different backgrounds together is seen as a crucial part of fixing the world’s problems. Research disciplines interpenetrate, providing a great area for cooperation. Life sciences and social sciences, and medical and legal sciences in particular, are a great example of such a combination.","PeriodicalId":15259,"journal":{"name":"Journal of Bioanalysis & Biomedicine","volume":"201 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76010618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}