Journal of autoimmunity最新文献

{"title":"Transcriptomic analyses of lung tissues reveal key genes associated with progression of systemic sclerosis-interstitial lung disease (SSc-ILD)","authors":"Yehya Al-Adwi ,&nbsp;Johanna Westra ,&nbsp;Harry van Goor ,&nbsp;Leon C. van Kempen ,&nbsp;Mohammed Osman ,&nbsp;C. Tji Gan ,&nbsp;Wim Timens ,&nbsp;Douwe J. Mulder","doi":"10.1016/j.jaut.2024.103297","DOIUrl":"10.1016/j.jaut.2024.103297","url":null,"abstract":"<div><h3>Objective</h3><p>Systemic sclerosis-interstitial lung disease (SSc-ILD) is the leading cause of death in SSc, affecting around 50 % of the patients. Lung tissue of patients with early-stage SSc-ILD is characterized by a predominant inflammatory response with inconspicuous fibrosis, which may progress to honeycombing fibrosis. Hence, a better understanding of the molecular mechanisms underpinning SSc-ILD pathogenesis is needed to improve treatment options and progression prediction. This transcriptomic study aims to reveal the differential gene expression between control (ctrl) lung tissue and inflammatory, prefibrotic and fibrotic lung tissue to capture progression of early to late phase SSc-ILD.</p></div><div><h3>Methods</h3><p>Twelve explanted lungs from patients with SSc-ILD were used to analyze gene expression from formalin-fixed paraffin-embedded lung tissues with varying stages of ILD (n = 18) and control lung tissue (n = 6). The SSc-ILD tissues were stratified into three ROIs: inflammatory, prefibrotic, and fibrotic using histological assessments to define a longitudinal simulation of early to late phases of SSc-ILD. The nanoString (nS) nCounter Human Fibrosis Panel was used to profile the transcriptome in the regions of interest. Validation of potential targetswas performed with immunohistochemistry in the same tissues that were used for transcriptome analysis.</p></div><div><h3>Results</h3><p>To validate our simulation model, we performed subgroup analysis that showed an incremental increase in pathway scores related to the severity of fibrosis. Ctrl vs SSc-ILD comparison demonstrated 24 differentially expressed genes, two of which had the most pronounced p-values. Cyclin-dependent kinase inhibitor (<em>cdkn2c</em>) was overexpressed (<em>P</em> = 0.00052) in SSc-ILD compared to ctrl, while expression of Pellino E3 ubiquitin-protein ligase 1 (<em>peli1</em>) showed lower expression (<em>P</em> = 0.0012). Additionally, in all four groups, <em>cdkn2c</em> and <em>peli1</em> gene expression showed an incremental increase and decrease, respectively. Immunohistochemistry of <em>cdkn2c</em> showed consistent results with the nS analysis.</p></div><div><h3>Conclusion</h3><p>More <em>cdkn2c</em> and less <em>peli1</em> expression were associated with more advanced stages of SSc-ILD on histologic assessment. We report the potential of the cell cycle inhibitor and senescence marker, <em>cdkn2c</em> (p18) to be associated with fibrosis progression.</p></div>","PeriodicalId":15245,"journal":{"name":"Journal of autoimmunity","volume":"148 ","pages":"Article 103297"},"PeriodicalIF":7.9,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0896841124001318/pdfft?md5=aa713571c16709788cf60e7237102da2&pid=1-s2.0-S0896841124001318-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Earlier vs. later time period of COVID-19 infection and emergent autoimmune signs, symptoms, and serologies","authors":"Emily G. Oakes ,&nbsp;Eilish Dillon ,&nbsp;Katherine A. Buhler ,&nbsp;Hongshu Guan ,&nbsp;Misti Paudel ,&nbsp;Kathryne Marks ,&nbsp;Ifeoluwakiisi Adejoorin ,&nbsp;Jeong Yee ,&nbsp;Jack Ellrodt ,&nbsp;Sara Tedeschi ,&nbsp;Jeffrey Sparks ,&nbsp;Siobhan M. Case ,&nbsp;Tiffany Hsu ,&nbsp;Daniel H. Solomon ,&nbsp;A. Helena Jonsson ,&nbsp;Roberta Vezza Alexander ,&nbsp;Deepak A. Rao ,&nbsp;May Y. Choi ,&nbsp;Karen H. Costenbader","doi":"10.1016/j.jaut.2024.103299","DOIUrl":"10.1016/j.jaut.2024.103299","url":null,"abstract":"<div><h3>Objective</h3><p>Autoantibodies and autoimmune diseases after SARS-CoV-2 infection are widely reported. Given evolving variants, milder infections, and increasing population vaccination, we hypothesized that SARS-CoV-2 infection earlier in the pandemic would be associated with more autoimmune connective tissue disease (CTD) symptoms and immunologic abnormalities.</p></div><div><h3>Methods</h3><p>Patients ≥18 years old with COVID-19 3/1/2020-8/15/2022 completed the CTD Screening Questionnaire and were tested for 27 autoimmune serologies, SARS-CoV-2 serologies, cell-bound complement activation products (CB-CAPs), and T and B lymphocyte immunophenotypes by flow cytometry. We assessed relationships between symptoms, serologies, and immunophenotypes in earlier (3/1/2020-1/31/2021) vs. later (2/1/2021-8/15/2022) periods, with different predominating SARS-CoV-2 viruses.</p></div><div><h3>Results</h3><p>57 subjects had earlier and 23 had later pandemic COVID-19. 35 % of earlier vs. 17 % of later pandemic patients had CTD symptoms (p 0.18). More patients were antinuclear antibody (ANA) positive (44 % vs. 13 %, p 0.01) and had lupus anticoagulant (11 % vs. 4 %, p 0.67). After adjustment for age, race, and sex, earlier (vs. later) COVID-19 was associated with increased ANA positivity (OR 4.60, 95%CI 1.17, 18.15). No subjects had positive CB-CAPs. T and B cell immunophenotypes and SARS-CoV-2 serologies did not differ by group. In heatmap analyses, higher autoantibody variety was seen among those with infection in the early pandemic.</p></div><div><h3>Conclusion</h3><p>In this sample, having COVID-19 infection in the earlier (pre-2/1/2021) vs. later pandemic was associated with more CTD symptoms, ANA positivity, and autoantibody reactivities. Earlier SARS-CoV-2 variants circulating in a less vaccinated population with less natural immunity may have been more immunogenic.</p></div>","PeriodicalId":15245,"journal":{"name":"Journal of autoimmunity","volume":"148 ","pages":"Article 103299"},"PeriodicalIF":7.9,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141881937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The binding of extracellular cyclophilin A to ACE2 and CD147 triggers psoriasis-like inflammation","authors":"Wenxian Yang ,&nbsp;Xiaoyuan Bai ,&nbsp;Xiaoxiao Jia ,&nbsp;Huizi Li ,&nbsp;Jie Min ,&nbsp;Heqiao Li ,&nbsp;Haoran Zhang ,&nbsp;Jianjing Zhou ,&nbsp;Yuna Zhao ,&nbsp;Wenjun Liu ,&nbsp;Haiming Xin ,&nbsp;Lei Sun","doi":"10.1016/j.jaut.2024.103293","DOIUrl":"10.1016/j.jaut.2024.103293","url":null,"abstract":"<div><p>Psoriasis is a chronic, proliferative, and inflammatory skin disease closely associated with inflammatory cytokine production. Cyclophilin A (CypA) is an important proinflammatory factor; however, its role in psoriasis remains unclear. The present data indicate that CypA levels are increased in the lesion skin and serum of patients with psoriasis, which is positively correlated with the psoriasis area severity index. Furthermore, extracellular CypA (eCypA) triggered psoriasis-like inflammatory responses in keratinocytes. Moreover, anti-CypA mAb significantly reduced pathological injury, keratinocyte proliferation, cytokine expression in imiquimod-induced mice. Notably, the therapeutic effect of anti-CypA mAb was better than that of the clinically used anti-IL-17A mAb and methotrexate. Mechanistically, eCypA binds to ACE2 and CD147 and is blocked by anti-CypA mAb. eCypA not only induces the dimerization and phosphorylation of ACE2 to trigger the JAK1/STAT3 signaling pathway for cytokine expression but also interacts with CD147 to promote PI3K/AKT/mTOR signaling-mediated keratinocyte proliferation. These findings demonstrate that the binding of eCypA to ACE2 and CD147 cooperatively triggers psoriasis-like inflammation and anti-CypA mAb is a promising candidate for the treatment of psoriasis.</p></div>","PeriodicalId":15245,"journal":{"name":"Journal of autoimmunity","volume":"148 ","pages":"Article 103293"},"PeriodicalIF":7.9,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0896841124001276/pdfft?md5=b3539965ed70a5597ba6eee9d53a3563&pid=1-s2.0-S0896841124001276-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141881988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shared aetiology underlying multiple sclerosis and other immune mediated inflammatory diseases: Swedish familial co-aggregation and large-scale genetic correlation analyses","authors":"Qianwen Liu ,&nbsp;Yuan Jiang ,&nbsp;Thomas Frisell ,&nbsp;Pernilla Stridh ,&nbsp;Klementy Shchetynsky ,&nbsp;Lars Alfredsson ,&nbsp;Ingrid Kockum ,&nbsp;Ali Manouchehrinia ,&nbsp;Xia Jiang","doi":"10.1016/j.jaut.2024.103294","DOIUrl":"10.1016/j.jaut.2024.103294","url":null,"abstract":"<div><h3>Background</h3><p>While multiple sclerosis (MS) affects less than 1 % of the general population, immune mediated inflammatory diseases (IMIDs) collectively influence 5–10 % of the population. Understanding familial co-aggregation of MS and other IMIDs carries important clinical and public health implications that will enable early detection and personalized treatment.</p></div><div><h3>Objective</h3><p>To estimate the familial association between MS and other IMIDs and to quantify their shared genetic basis.</p></div><div><h3>Design</h3><p>Register-based multi-generational nested case-control familial co-aggregation study and genetic correlation study.</p></div><div><h3>Setting</h3><p>Sweden.</p></div><div><h3>Participants</h3><p>24,995 individuals with MS matched with 253,870 controls and 1,283,502 first-degree relatives (mothers, fathers, full siblings, and offspring) for familial co-aggregation analysis; population of European ancestry for genetic correlation analysis.</p></div><div><h3>Measurements</h3><p>Logistic regressions with adjustment for covariates were used to estimate the odds ratios (ORs) of developing MS in individuals with first-degree relatives diagnosed with IMIDs compared to those without such family history. Pairwise genome-wide genetic correlations were estimated with linkage-disequilibrium score regression.</p></div><div><h3>Results</h3><p>We observed an OR for familial co-aggregation of MS of 1.09 (95 % confidence interval (95%CI) = 1.07−1.11) in families with IMIDs history compared to families without. The association remained broadly consistent after stratification by sex concordance of relative pairs and by kinships. 18 IMID subtypes showed a familial association with MS, 7 of which including other acute widespread myelin destruction, encephalitis or myelitis or encephalomyelitis, inflammatory bowel disease, autoimmune thyroid diseases, systemic lupus erythematosus, other inflammatory system diseases, and sarcoidosis withstood multiple correction. Genetic correlations further revealed a shared genetic basis between 7 IMID subtypes with MS.</p></div><div><h3>Conclusion</h3><p>We demonstrated a modest familial co-aggregation of MS with several IMIDs, and such association is likely due to shared genetic factors.</p></div>","PeriodicalId":15245,"journal":{"name":"Journal of autoimmunity","volume":"148 ","pages":"Article 103294"},"PeriodicalIF":7.9,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0896841124001288/pdfft?md5=1f67eefcafd5b7fc808603cea2f02fef&pid=1-s2.0-S0896841124001288-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141859867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiologic and genetic associations between primary biliary cholangitis and extrahepatic rheumatic diseases","authors":"Qiwei Qian ,&nbsp;Yi Wu ,&nbsp;Nana Cui ,&nbsp;Yikang Li ,&nbsp;Yujie Zhou ,&nbsp;You Li ,&nbsp;Min Lian ,&nbsp;Xiao Xiao ,&nbsp;Qi Miao ,&nbsp;Zhengrui You ,&nbsp;Qixia Wang ,&nbsp;Yongyong Shi ,&nbsp;Heather J. Cordell ,&nbsp;Suraj Timilsina ,&nbsp;M. Eric Gershwin ,&nbsp;Zhiqiang Li ,&nbsp;Xiong Ma ,&nbsp;Ruqi Tang","doi":"10.1016/j.jaut.2024.103289","DOIUrl":"10.1016/j.jaut.2024.103289","url":null,"abstract":"<div><p>Patients with primary biliary cholangitis (PBC) commonly experience extrahepatic rheumatic diseases. However, the epidemiologic and genetic associations as well as causal relationship between PBC and these extrahepatic conditions remain undetermined. In this study, we first conducted systematic review and meta-analyses by analyzing 73 studies comprising 334,963 participants across 17 countries and found strong phenotypic associations between PBC and rheumatic diseases. Next, we utilized large-scale genome-wide association study summary data to define the shared genetic architecture between PBC and rheumatic diseases, including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), systemic sclerosis (SSc) and Sjögren's syndrome (SS). We observed significant genetic correlations between PBC and each of the four rheumatic diseases. Pleiotropy and heritability enrichment analysis suggested the involvement of humoral immunity and interferon-associated processes for the comorbidity. Of note, we identified four variants shared between PBC and RA (rs80200208), SLE (rs9843053), and SSc (rs27524, rs3873182) using cross-trait meta-analysis. Additionally, several pleotropic loci for PBC and rheumatic diseases were found to share causal variants with gut microbes possessing immunoregulatory functions. Finally, Mendelian randomization revealed consistent evidence for a causal effect of PBC on RA, SLE, SSc, and SS, but no or inconsistent evidence for a causal effect of extrahepatic rheumatic diseases on PBC. Our study reveals a profound genetic overlap and causal relationships between PBC and extrahepatic rheumatic diseases, thus providing insights into shared biological mechanisms and novel therapeutic interventions.</p></div>","PeriodicalId":15245,"journal":{"name":"Journal of autoimmunity","volume":"148 ","pages":"Article 103289"},"PeriodicalIF":7.9,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141766131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin synergistically potentiates the effect of methylprednisolone on reducing neuroinflammation in the experimental autoimmune encephalomyelitis mouse model of multiple sclerosis","authors":"Ana Isabel Álvarez-López ,&nbsp;Nuria Álvarez-Sánchez ,&nbsp;Ivan Cruz-Chamorro ,&nbsp;Guillermo Santos-Sánchez ,&nbsp;Eduardo Ponce-España ,&nbsp;Ignacio Bejarano ,&nbsp;Patricia Judith Lardone ,&nbsp;Antonio Carrillo-Vico","doi":"10.1016/j.jaut.2024.103298","DOIUrl":"10.1016/j.jaut.2024.103298","url":null,"abstract":"<div><p>Multiple sclerosis (MS) is an autoimmune neurodegenerative disease of unknown etiology characterized by infiltration of encephalitogenic cells in the central nervous system (CNS) resulting in the presence of multifocal areas of demyelination leading to neurodegeneration. The infiltrated immune cells population is composed mainly of effector CD4⁺ and CD8⁺ T lymphocytes, B cells, macrophages, and dendritic cells that secrete pro-inflammatory factors that eventually damage myelin leading to axonal damage. The most common clinical form of MS is relapsing-remitting (RR), characterized by neuroinflammatory episodes followed by partial or total recovery of neurological deficits. The first-line treatment for RRMS relapses is a high dose of glucocorticoids, especially methylprednisolone, for three to five consecutive days. Several studies have reported the beneficial effects of melatonin in the context of neuroinflammation associated with MS or experimental autoimmune encephalomyelitis (EAE), the preclinical model for MS. Therefore, the objective of this study was to evaluate the effect of the combined treatment of melatonin and methylprednisolone on the neuroinflammatory response associated with the EAE development. This study shows for the first time the protective synergistic effect of co-treatment with melatonin and methylprednisolone on reducing the severity of EAE by decreasing CD4 lymphocytes, B cells, macrophages and dendritic cells in the CNS, as well as modulating the population of infiltrated T and B cells toward regulatory phenotypes to the detriment of pro-inflammatory effector functions. In addition to the potentiation of the protective role of methylprednisolone, treatment with melatonin from the clinical onset of EAE improves the natural course of the EAE and the response to a subsequent treatment with methylprednisolone in a later relapse of the disease, pointing melatonin as potential therapeutic tool in combination with methylprednisolone for the treatment of relapses in MS.</p></div>","PeriodicalId":15245,"journal":{"name":"Journal of autoimmunity","volume":"148 ","pages":"Article 103298"},"PeriodicalIF":7.9,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S089684112400132X/pdfft?md5=951f35a4ccfd85922a4071f095a390df&pid=1-s2.0-S089684112400132X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141773714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perivascular B cells link intestinal angiogenesis to immunity and to the gut-brain axis during neuroinflammation","authors":"Benjamin Peter ,&nbsp;Jessica Rebeaud ,&nbsp;Solenne Vigne ,&nbsp;Valentine Bressoud ,&nbsp;Nicholas Phillips ,&nbsp;Florian Ruiz ,&nbsp;Tatiana V. Petrova ,&nbsp;Jeremiah Bernier-Latmani ,&nbsp;Caroline Pot","doi":"10.1016/j.jaut.2024.103292","DOIUrl":"10.1016/j.jaut.2024.103292","url":null,"abstract":"<div><p>Disruption of gut barrier function and intestinal immune cell homeostasis are increasingly considered critical players in pathogenesis of extra-intestinal inflammatory diseases, including multiple sclerosis (MS) and its prototypical animal model, the experimental autoimmune encephalomyelitis (EAE). Breakdown of epithelial barriers increases intestinal permeability and systemic dissemination of microbiota-derived molecules. However, whether the gut-vascular barrier (GVB) is altered during EAE has not been reported. Here, we demonstrate that endothelial cell proliferation and vessel permeability increase before EAE clinical onset, leading to vascular remodeling and expansion of intestinal villi capillary bed during disease symptomatic phase in an antigen-independent manner. Concomitant to onset of angiogenesis observed prior to neurological symptoms, we identify an increase of intestinal perivascular immune cells characterized by the surface marker lymphatic vessel endothelial hyaluronic acid receptor 1 (LYVE-1). LYVE-1⁺ is expressed more frequently on B cells that show high levels of CD73 and have proangiogenic properties. B cell depletion was sufficient to mitigate enteric blood endothelial cell proliferation following immunization for EAE. In conclusion, we propose that altered intestinal vasculature driven by a specialized LYVE-1⁺ B cell subset promotes angiogenesis and that loss of GVB function is implicated in EAE development and autoimmunity.</p></div>","PeriodicalId":15245,"journal":{"name":"Journal of autoimmunity","volume":"148 ","pages":"Article 103292"},"PeriodicalIF":7.9,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141773717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune-mediated necrotizing myopathy: A comprehensive review of the pathogenesis, clinical features, and treatments","authors":"Changpei Li ,&nbsp;Hongjiang Liu ,&nbsp;Leiyi Yang ,&nbsp;Ruiting Liu ,&nbsp;Geng Yin ,&nbsp;Qibing Xie","doi":"10.1016/j.jaut.2024.103286","DOIUrl":"10.1016/j.jaut.2024.103286","url":null,"abstract":"<div><p>Immune-mediated necrotizing myopathy (IMNM) is a rare and newly recognized autoimmune disease within the spectrum of idiopathic inflammatory myopathies. It is characterized by myositis-specific autoantibodies, elevated serum creatine kinase levels, inflammatory infiltrate, and weakness. IMNM can be classified into three subtypes based on the presence or absence of specific autoantibodies: anti-signal recognition particle myositis, anti-3-hydroxy-3-methylglutaryl-coenzyme A reductase myositis, and seronegative IMNM. In recent years, IMNM has gained increasing attention and emerged as a research hotspot. Recent studies have suggested that the pathogenesis of IMNM is linked to aberrant activation of immune system, including immune responses mediated by antibodies, complement, and immune cells, particularly macrophages, as well as abnormal release of inflammatory factors. Non-immune mechanisms such as autophagy and endoplasmic reticulum stress also participate in this process. Additionally, genetic variations associated with IMNM have been identified, providing new insights into the genetic mechanisms of the disease. Progress has also been made in IMNM treatment research, including the use of immunosuppressants and the development of biologics. Despite the challenges in understanding the etiology and treatment of IMNM, the latest research findings offer important guidance and insights for delving deeper into the disease's pathogenic mechanisms and identifying new therapeutic strategies.</p></div>","PeriodicalId":15245,"journal":{"name":"Journal of autoimmunity","volume":"148 ","pages":"Article 103286"},"PeriodicalIF":7.9,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-fat diet modulates bile acid composition and gut microbiota, affecting severe cholangitis and cirrhotic change in murine primary biliary cholangitis","authors":"Masahiro Umemura ,&nbsp;Akira Honda ,&nbsp;Maho Yamashita ,&nbsp;Takeshi Chida ,&nbsp;Hidenao Noritake ,&nbsp;Kenta Yamamoto ,&nbsp;Takashi Honda ,&nbsp;Mayuko Ichimura-Shimizu ,&nbsp;Koichi Tsuneyama ,&nbsp;Teruo Miyazaki ,&nbsp;Nobuhito Kurono ,&nbsp;Patrick S.C. Leung ,&nbsp;M. Eric Gershwin ,&nbsp;Takafumi Suda ,&nbsp;Kazuhito Kawata","doi":"10.1016/j.jaut.2024.103287","DOIUrl":"10.1016/j.jaut.2024.103287","url":null,"abstract":"<div><p>Increasing evidence suggests that, in addition to a loss of tolerance, bile acid (BA) modulates the natural history of primary biliary cholangitis (PBC). We focused on the impacts of dietary changes on the immunopathology of PBC, along with alterations in BA composition and gut microbiota. In this study, we have taken advantage of our unique PBC model, a <em>Cyp2c70/Cyp2a12</em> double knockout (DKO), which includes a human-like BA composition, and develops progressive cholangitis following immunization with the PDC-E2 mimic, 2-octynoic acid (2OA). We compared the effects of a ten-week high-fat diet (HFD) (60 % kcal from fat) and a normal diet (ND) on 2OA-treated DKO mice. Importantly, we report that 2OA-treated DKO mice fed HFD had significantly exacerbated cholangitis, leading to cirrhosis, with increased hepatic expression of Th1 cytokines/chemokines and hepatic fibrotic markers. Serum lithocholic acid (LCA) levels and the ratio of chenodeoxycholic acid (CDCA)-derived BAs to cholic acid-derived BAs were significantly increased by HFD. This was also associated with downregulated expression of key regulators of BA synthesis, including <em>Cyp8b1</em>, <em>Cyp3a11</em>, and <em>Sult2a1</em>. In addition, there were increases in the relative abundances of <em>Acetatifactor</em> and <em>Lactococcus</em> and decreases in <em>Desulfovibrio</em> and <em>Lachnospiraceae_NK4A136_group</em>, which corresponded to the abundances of CDCA and LCA. In conclusion, HFD and HFD-induced alterations in the gut microbiota modulate BA composition and nuclear receptor activation, leading to cirrhotic change in this murine PBC model. These findings have significant implications for understanding the progression of human PBC.</p></div>","PeriodicalId":15245,"journal":{"name":"Journal of autoimmunity","volume":"148 ","pages":"Article 103287"},"PeriodicalIF":7.9,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional metagenomic analysis reveals potential inflammatory triggers associated with genetic risk for autoimmune disease","authors":"Meghan A. Berryman ,&nbsp;Jorma Ilonen ,&nbsp;Eric W. Triplett ,&nbsp;Johnny Ludvigsson","doi":"10.1016/j.jaut.2024.103290","DOIUrl":"10.1016/j.jaut.2024.103290","url":null,"abstract":"<div><p>To assess functional differences between the microbiomes of individuals with autoimmune risk-associated human leukocyte antigen (HLA) genetics and autoimmune protection-associated HLA, we performed a metagenomic analysis of stool samples from 72 infants in the All Babies in Southeast Sweden general-population cohort and assessed haplotype-peptide binding affinities. Infants with risk-associated HLA DR3-DQ2.5 and DR4-DQ8 had a higher abundance of known pathogen-associated molecular patterns and virulence related genes than infants with protection-associated HLA DR15-DQ6.2. However, there was limited overlap in the type of inflammatory trigger between risk groups. Supported by a high Firmicutes/Bacteroides ratio and differentially abundant flagellated species, genes related to the synthesis of flagella were prominent in those with HLA DR3-DQ2.5. However, this haplotype had a significantly lower likelihood of binding affinity to flagellin peptides. O-antigen biosynthesis genes were significantly correlated with the risk genotypes and absent from protective genotype association, supported by the differential abundance of gram-negative bacteria seen in the risk-associated groups. Genes related to vitamin B biosynthesis stood out in higher abundance in infants with HLA DR3-DQ2.5/DR4-DQ8 heterozygosity compared to those with autoimmune-protective genetics. <em>Prevotella</em> species and genus were significantly abundant in all infant groups with high risk for autoimmune disease. The potential inflammatory triggers associated with genetic risk for autoimmunity have significant implications. These results suggest that certain HLA haplotypes may be creating the opportunity for dysbiosis and subsequent inflammation early in life by clearing beneficial microbes or not clearing proinflammatory microbes. This HLA gatekeeping may prevent genetically at-risk individuals from benefiting from probiotic therapies by restricting the colonization of those beneficial bacteria.</p></div>","PeriodicalId":15245,"journal":{"name":"Journal of autoimmunity","volume":"148 ","pages":"Article 103290"},"PeriodicalIF":7.9,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0896841124001240/pdfft?md5=6506d69743b2c76a7c146de4e5c9fa9a&pid=1-s2.0-S0896841124001240-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}