Journal of cell science最新文献_第8页

Direct targeting of host microtubule and actin cytoskeletons by a chlamydial pathogenic effector protein. 衣原体致病效应蛋白直接靶向宿主微管和肌动蛋白细胞骨架。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-09-01 Epub Date: 2024-09-06 DOI: 10.1242/jcs.263450

Mona Höhler, Abel R Alcázar-Román, Katharina Schenk, Mac Pholo Aguirre-Huamani, Corinna Braun, Rafat Zrieq, Katja Mölleken, Johannes H Hegemann, Ursula Fleig

{"title":"Direct targeting of host microtubule and actin cytoskeletons by a chlamydial pathogenic effector protein.","authors":"Mona Höhler, Abel R Alcázar-Román, Katharina Schenk, Mac Pholo Aguirre-Huamani, Corinna Braun, Rafat Zrieq, Katja Mölleken, Johannes H Hegemann, Ursula Fleig","doi":"10.1242/jcs.263450","DOIUrl":"10.1242/jcs.263450","url":null,"abstract":"To propagate within a eukaryotic cell, pathogenic bacteria hijack and remodulate host cell functions. The Gram-negative obligate intracellular Chlamydiaceae, which pose a serious threat to human and animal health, attach to host cells and inject effector proteins that reprogram host cell machineries. Members of the conserved chlamydial TarP family have been characterized as major early effectors that bind to and remodel the host actin cytoskeleton. We now describe a new function for the Chlamydia pneumoniae TarP member CPn0572, namely the ability to bind and alter the microtubule cytoskeleton. Thus, CPn0572 is unique in being the only prokaryotic protein that directly modulates both dynamic cytoskeletons of a eukaryotic cell. Ectopically expressed GFP-CPn0572 associates in a dose-independent manner with either cytoskeleton singly or simultaneously. In vitro, CPn0572 binds directly to microtubules. Expression of a microtubule-only CPn0572 variant resulted in the formation of an aberrantly thick, stabilized microtubule network. Intriguingly, during infection, secreted CPn0572 also colocalized with altered microtubules, suggesting that this protein also affects microtubule dynamics during infection. Our analysis points to a crosstalk between actin and microtubule cytoskeletons via chlamydial CPn0572.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11444262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolving circuitries in plant signaling cascades. 植物信号级联中不断演变的回路。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-09-01 Epub Date: 2024-09-06 DOI: 10.1242/jcs.261712

Jaccoline M S Zegers, Iker Irisarri, Sophie de Vries, Jan de Vries

引用次数: 0

Nucleocytoplasmic transport senses mechanical forces independently of cell density in cell monolayers. 细胞单层中的核胞质运输感知力学与细胞密度无关。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-09-01 Epub Date: 2024-09-09 DOI: 10.1242/jcs.262363

Ignasi Granero-Moya, Valeria Venturini, Guillaume Belthier, Bart Groenen, Marc Molina-Jordán, Miguel González-Martín, Xavier Trepat, Jacco van Rheenen, Ion Andreu, Pere Roca-Cusachs

{"title":"Nucleocytoplasmic transport senses mechanical forces independently of cell density in cell monolayers.","authors":"Ignasi Granero-Moya, Valeria Venturini, Guillaume Belthier, Bart Groenen, Marc Molina-Jordán, Miguel González-Martín, Xavier Trepat, Jacco van Rheenen, Ion Andreu, Pere Roca-Cusachs","doi":"10.1242/jcs.262363","DOIUrl":"10.1242/jcs.262363","url":null,"abstract":"Cells sense and respond to mechanical forces through mechanotransduction, which regulates processes in health and disease. In single adhesive cells, mechanotransduction involves the transmission of force from the extracellular matrix to the cell nucleus, where it affects nucleocytoplasmic transport (NCT) and the subsequent nuclear localization of transcriptional regulators, such as YAP (also known as YAP1). However, if and how NCT is mechanosensitive in multicellular systems is unclear. Here, we characterize and use a fluorescent sensor of nucleocytoplasmic transport (Sencyt) and demonstrate that NCT responds to mechanical forces but not cell density in cell monolayers. Using monolayers of both epithelial and mesenchymal phenotype, we show that NCT is altered in response both to osmotic shocks and to the inhibition of cell contractility. Furthermore, NCT correlates with the degree of nuclear deformation measured through nuclear solidity, a shape parameter related to nuclear envelope tension. In contrast, YAP is sensitive to cell density, showing that the YAP response to cell-cell contacts is not via a mere mechanical effect of NCT. Our results demonstrate the generality of the mechanical regulation of NCT.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of kinesin-1 in neuronal dense core vesicle transport, locomotion and lifespan regulation in C. elegans. 驱动蛋白-1在秀丽隐杆线虫神经元致密核心囊泡运输、运动和寿命调节中的作用

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-09-01 Epub Date: 2024-09-06 DOI: 10.1242/jcs.262148

Anna Gavrilova, Astrid Boström, Nickolay Korabel, Sergei Fedotov, Gino B Poulin, Victoria J Allan

{"title":"The role of kinesin-1 in neuronal dense core vesicle transport, locomotion and lifespan regulation in C. elegans.","authors":"Anna Gavrilova, Astrid Boström, Nickolay Korabel, Sergei Fedotov, Gino B Poulin, Victoria J Allan","doi":"10.1242/jcs.262148","DOIUrl":"10.1242/jcs.262148","url":null,"abstract":"Fast axonal transport is crucial for neuronal function and is driven by kinesins and cytoplasmic dynein. Here, we investigated the role of kinesin-1 in dense core vesicle (DCV) transport in C. elegans, using mutants in the kinesin light chains (klc-1 and klc-2) and the motor subunit (unc-116) expressing an ida-1::gfp transgene that labels DCVs. DCV transport in both directions was greatly impaired in an unc-116 mutant and had reduced velocity in a klc-2 mutant. In contrast, the speed of retrograde DCV transport was increased in a klc-1 mutant whereas anterograde transport was unaffected. We identified striking differences between the klc mutants in their effects on worm locomotion and responses to drugs affecting neuromuscular junction activity. We also determined lifespan, finding that unc-116 mutant was short-lived whereas the klc single mutant lifespan was wild type. The ida-1::gfp transgenic strain was also short-lived, but surprisingly, klc-1 and klc-2 extended the ida-1::gfp lifespan beyond that of wild type. Our findings suggest that kinesin-1 not only influences anterograde and retrograde DCV transport but is also involved in regulating lifespan and locomotion, with the two kinesin light chains playing distinct roles.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational modeling establishes mechanotransduction as a potent modulator of the mammalian circadian clock. 计算模型证实机械传导是哺乳动物昼夜节律时钟的有效调节器。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-09-01 Epub Date: 2024-09-09 DOI: 10.1242/jcs.261782

Emmet A Francis, Padmini Rangamani

{"title":"Computational modeling establishes mechanotransduction as a potent modulator of the mammalian circadian clock.","authors":"Emmet A Francis, Padmini Rangamani","doi":"10.1242/jcs.261782","DOIUrl":"10.1242/jcs.261782","url":null,"abstract":"Mechanotransduction, which is the integration of mechanical signals from the external environment of a cell to changes in intracellular signaling, governs many cellular functions. Recent studies have shown that the mechanical state of the cell is also coupled to the cellular circadian clock. To investigate possible interactions between circadian rhythms and cellular mechanotransduction, we have developed a computational model that integrates the two pathways. We postulated that translocation of the transcriptional regulators MRTF (herein referring to both MRTF-A and MRTF-B), YAP and TAZ (also known as YAP1 and WWTR1, respectively; collectively denoted YAP/TAZ) into the nucleus leads to altered expression of circadian proteins. Simulations from our model predict that lower levels of cytoskeletal activity are associated with longer circadian oscillation periods and higher oscillation amplitudes, which is consistent with recent experimental observations. Furthermore, accumulation of YAP/TAZ and MRTF in the nucleus causes circadian oscillations to decay in our model. These effects hold both at the single-cell level and within a population-level framework. Finally, we investigated the effects of mutations in YAP or lamin A, the latter of which result in a class of diseases known as laminopathies. In silico, oscillations in circadian proteins are substantially weaker in populations of cells with mutations in YAP or lamin A, suggesting that defects in mechanotransduction can disrupt the circadian clock in certain disease states; however, reducing substrate stiffness in the model restores normal oscillatory behavior, suggesting a possible compensatory mechanism. Thus, our study identifies that mechanotransduction could be a potent modulatory cue for cellular clocks and that this crosstalk can be leveraged to rescue the circadian clock in disease states.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The FAM114A proteins are adaptors for the recycling of Golgi enzymes. FAM114A 蛋白是高尔基体酶再循环的适配器。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-09-01 Epub Date: 2024-09-06 DOI: 10.1242/jcs.262160

Lawrence G Welch, Nadine Muschalik, Sean Munro

{"title":"The FAM114A proteins are adaptors for the recycling of Golgi enzymes.","authors":"Lawrence G Welch, Nadine Muschalik, Sean Munro","doi":"10.1242/jcs.262160","DOIUrl":"10.1242/jcs.262160","url":null,"abstract":"Golgi-resident enzymes remain in place while their substrates flow through from the endoplasmic reticulum to elsewhere in the cell. COPI-coated vesicles bud from the Golgi to recycle Golgi residents to earlier cisternae. Different enzymes are present in different parts of the stack, and one COPI adaptor protein, GOLPH3, acts to recruit enzymes into vesicles in part of the stack. Here, we used proximity biotinylation to identify further components of intra-Golgi vesicles and found FAM114A2, a cytosolic protein. Affinity chromatography with FAM114A2, and its paralogue FAM114A1, showed that they bind to Golgi-resident membrane proteins, with membrane-proximal basic residues in the cytoplasmic tail being sufficient for the interaction. Deletion of both proteins from U2OS cells did not cause substantial defects in Golgi function. However, a Drosophila orthologue of these proteins (CG9590/FAM114A) is also localised to the Golgi and binds directly to COPI. Drosophila mutants lacking FAM114A have defects in glycosylation of glue proteins in the salivary gland. Thus, the FAM114A proteins bind Golgi enzymes and are candidate adaptors to contribute specificity to COPI vesicle recycling in the Golgi stack.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remembering Andrew Fry (1966-2024). 缅怀安德鲁-弗莱（1966-2024）。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-09-01 Epub Date: 2024-09-06 DOI: 10.1242/jcs.263478

Richard Bayliss, Tim Fry, Robert Mahen, Sue Shackleton, Kayoko Tanaka

引用次数: 0

Controversy in mechanotransduction - the role of endothelial cell-cell junctions in fluid shear stress sensing. 机械传导中的争议：内皮细胞-细胞连接在流体剪切应力传感中的作用。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-09-01 Epub Date: 2024-09-09 DOI: 10.1242/jcs.262348

Shaka X, Claire Aitken, Vedanta Mehta, Blanca Tardajos-Ayllon, Jovana Serbanovic-Canic, Jiayu Zhu, Bernadette Miao, Ellie Tzima, Paul Evans, Yun Fang, Martin A Schwartz

引用次数: 0

The proximal centriole-like structure maintains nucleus-centriole architecture in sperm. 近端中心粒样结构维持精子中的核-中心粒结构。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-09-01 Epub Date: 2024-09-06 DOI: 10.1242/jcs.262311

Danielle B Buglak, Kathleen H M Holmes, Brian J Galletta, Nasser M Rusan

{"title":"The proximal centriole-like structure maintains nucleus-centriole architecture in sperm.","authors":"Danielle B Buglak, Kathleen H M Holmes, Brian J Galletta, Nasser M Rusan","doi":"10.1242/jcs.262311","DOIUrl":"10.1242/jcs.262311","url":null,"abstract":"Proper connection between the sperm head and tail is critical for sperm motility and fertilization. Head-tail linkage is mediated by the head-tail coupling apparatus (HTCA), which secures the axoneme (tail) to the nucleus (head). However, the molecular architecture of the HTCA is poorly understood. Here, we use Drosophila to investigate formation and remodeling of the HTCA throughout spermiogenesis by visualizing key components of this complex. Using structured illumination microscopy, we demonstrate that key HTCA proteins Spag4 and Yuri form a 'centriole cap' that surrounds the centriole (or basal body) as it invaginates into the surface of the nucleus. As development progresses, the centriole is laterally displaced to the side of the nucleus while the HTCA expands under the nucleus, forming what we term the 'nuclear shelf'. We next show that the proximal centriole-like (PCL) structure is positioned under the nuclear shelf, functioning as a crucial stabilizer of centriole-nucleus attachment. Together, our data indicate that the HTCA is a complex, multi-point attachment site that simultaneously engages the PCL, the centriole and the nucleus to ensure proper head-tail connection during late-stage spermiogenesis.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intermediate filaments at a glance. 中间纤维一览

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-08-15 Epub Date: 2024-08-29 DOI: 10.1242/jcs.261386

Leila S Coelho-Rato, Sepideh Parvanian, Sarka Andrs Salajkova, Ohad Medalia, John E Eriksson

引用次数: 0