Journal of cell science最新文献_第8页

Cajal body formation is regulated by coilin SUMOylation. Cajal体的形成受coilin SUMOylation调节。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-11 DOI: 10.1242/jcs.263447

Sara K Tucker, Douglas M McLaurin, Michael D Hebert

引用次数: 0

'Iterative Bleaching Extends Multiplexity' facilitates simultaneous identification of all major retinal cell types. 迭代漂白扩展复用技术（IBEX）可同时识别所有主要视网膜细胞类型。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-10 DOI: 10.1242/jcs.263407

Aanandita A Kothurkar, Gregory S Patient, Nicole C L Noel, Aleksandra M Krzywańska, Brittany J Carr, Colin J Chu, Ryan B MacDonald

{"title":"'Iterative Bleaching Extends Multiplexity' facilitates simultaneous identification of all major retinal cell types.","authors":"Aanandita A Kothurkar, Gregory S Patient, Nicole C L Noel, Aleksandra M Krzywańska, Brittany J Carr, Colin J Chu, Ryan B MacDonald","doi":"10.1242/jcs.263407","DOIUrl":"10.1242/jcs.263407","url":null,"abstract":"To understand the multicellular composition of tissues, and how it is altered during development, ageing and/or disease, we must visualise the complete cellular landscape. Currently, this is hindered by our limited ability to combine multiple cellular markers. To overcome this, we adapted a highly multiplexed immunofluorescence (IF) technique called 'Iterative Bleaching Extends Multiplexity' (IBEX) to the zebrafish retina. We optimised fluorescent antibody micro-conjugation to perform sequential rounds of labelling on a single tissue to simultaneously visualise all major retinal cell types with 11 cell-specific antibodies. We further adapted IBEX to be compatible with fluorescent transgenic reporter lines, in situ hybridisation chain reaction (HCR), and whole-mount immunofluorescence (WMIF). We applied IBEX at multiple stages to study the spatial and temporal relationships between glia and neurons during retinal development. Finally, we demonstrate the utility of IBEX across species by testing it on the turquoise killifish (Nothobranchius furzeri) and African clawed frog (Xenopus laevis) to glean large amounts of information from precious tissues. These techniques will revolutionise our ability to visualise multiple cell types in any organism where antibodies are readily available.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proximity labeling reveals interactions necessary to maintain the distinct apical domains of Drosophila photoreceptors. 近距离标记揭示了维持果蝇感光器独特顶端结构所需的相互作用。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-11 DOI: 10.1242/jcs.262223

Lalitha Sastry, Johnathan Rylee, Simpla Mahato, Andrew C Zelhof

{"title":"Proximity labeling reveals interactions necessary to maintain the distinct apical domains of Drosophila photoreceptors.","authors":"Lalitha Sastry, Johnathan Rylee, Simpla Mahato, Andrew C Zelhof","doi":"10.1242/jcs.262223","DOIUrl":"10.1242/jcs.262223","url":null,"abstract":"Specialized membrane and cortical protein regions are common features of cells and are utilized to isolate differential cellular functions. In Drosophila photoreceptors, the apical membrane domain is defined by two distinct morphological membranes: the rhabdomere microvilli and the stalk membrane. To define the apical cortical protein complexes, we performed proximity labeling screens utilizing the rhabdomeric-specific protein PIP82 as bait. We found that the PIP82 interactome is enriched in actin-binding and cytoskeleton proteins, as well as proteins for cellular trafficking. Analysis of one target, Bifocal, with PIP82 revealed two independent pathways for localization to the rhabdomeric membrane and an additional mechanism of crosstalk between the protein complexes of the rhabdomeric and stalk membranes. The loss of Bifocal, and enhancement in the PIP82, bifocal double mutant, resulted in the additional distribution of Crumbs, an apical stalk membrane protein, to the lateral basal photoreceptor membrane. This phenotype was recapitulated by the knockdown of the catalytic subunit of Protein phosphatase 1, a known interactor with Bifocal. Taken together, these results expand our understanding of the molecular mechanisms underlying the generation of the two distinct photoreceptor apical domains.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemomechanical regulation of EZH2 localization controls epithelial-mesenchymal transition. EZH2定位的化学机械调控控制着上皮-间质转化。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-15 Epub Date: 2024-11-25 DOI: 10.1242/jcs.262190

Jessica L Sacco, Zachary T Vaneman, Ava Self, Elix Sumner, Stella Kibinda, Chinmay S Sankhe, Esther W Gomez

{"title":"Chemomechanical regulation of EZH2 localization controls epithelial-mesenchymal transition.","authors":"Jessica L Sacco, Zachary T Vaneman, Ava Self, Elix Sumner, Stella Kibinda, Chinmay S Sankhe, Esther W Gomez","doi":"10.1242/jcs.262190","DOIUrl":"10.1242/jcs.262190","url":null,"abstract":"The methyltransferase enhancer of zeste homolog 2 (EZH2) regulates gene expression, and aberrant EZH2 expression and signaling can drive fibrosis and cancer. However, it is not clear how chemical and mechanical signals are integrated to regulate EZH2 and gene expression. We show that culture of cells on stiff matrices in concert with transforming growth factor (TGF)-β1 promotes nuclear localization of EZH2 and an increase in the levels of the corresponding histone modification, H3K27me3, thereby regulating gene expression. EZH2 activity and expression are required for TGFβ1- and stiffness-induced increases in H3K27me3 levels as well as for morphological and gene expression changes associated with epithelial-mesenchymal transition (EMT). Inhibition of Rho associated kinase (ROCK) proteins or myosin II signaling attenuates TGFβ1-induced nuclear localization of EZH2 and decreases H3K27me3 levels in cells cultured on stiff substrata, suggesting that cellular contractility, in concert with a major cancer signaling regulator TGFβ1, modulates EZH2 subcellular localization. These findings provide a contractility-dependent mechanism by which matrix stiffness and TGFβ1 together mediate EZH2 signaling to promote EMT.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ImmunoCellCycle-ID - a high-precision immunofluorescence-based method for cell cycle identification. ImmunoCellCycle-ID--一种基于免疫荧光的高精度细胞周期鉴定方法。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-15 Epub Date: 2024-11-20 DOI: 10.1242/jcs.263414

Yu-Lin Chen, Yu-Chia Chen, Aussie Suzuki

引用次数: 0

DRAK2 regulates myosin light chain phosphorylation in T cells. DRAK2 可调节 T 细胞中肌球蛋白轻链的磷酸化。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-15 Epub Date: 2024-11-20 DOI: 10.1242/jcs.261813

Benjamin A Wilander, Tarsha L Harris, Alexandra H Mandarano, Cliff S Guy, Mollie S Prater, Shondra M Pruett-Miller, Stacey K Ogden, Maureen A McGargill

{"title":"DRAK2 regulates myosin light chain phosphorylation in T cells.","authors":"Benjamin A Wilander, Tarsha L Harris, Alexandra H Mandarano, Cliff S Guy, Mollie S Prater, Shondra M Pruett-Miller, Stacey K Ogden, Maureen A McGargill","doi":"10.1242/jcs.261813","DOIUrl":"10.1242/jcs.261813","url":null,"abstract":"Death-associated protein kinase-related apoptosis-inducing kinase-2 (DRAK2; also known as STK17B) is a serine/threonine kinase expressed in T cells. Drak2-deficient (Drak2-/-) mice respond effectively to tumors and pathogens while displaying resistance to T cell-mediated autoimmune disease. However, the molecular mechanisms by which DRAK2 impacts T cell function remain unclear. Gaining further insight into the function of DRAK2 in T cells will shed light on differentially regulated pathways in autoreactive and pathogen-specific T cells, which is crucial for improving autoimmune therapies. Here, we demonstrate that DRAK2 contributes to activation of myosin light chain (MLC2, encoded by Myl2) in both murine and human T cells. In the absence of Drak2, the amount of polymerized actin was decreased, suggesting that DRAK2 modulates actomyosin dynamics. We further show that myosin-dependent T cell functions, such as migration, T cell receptor microcluster accumulation, and conjugation to antigen presenting cells are decreased in the absence of Drak2. These findings reveal that DRAK2 plays an important role in regulating MLC activation within T cells.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The lysosomal lipid transporter LIMP-2 is part of lysosome-ER STARD3-VAPB-dependent contact sites. 溶酶体脂质转运体 LIMP-2/SCARB2 是溶酶体-内质网 STARD3-VAPB 依赖性接触点的一部分。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-15 Epub Date: 2024-11-26 DOI: 10.1242/jcs.261810

Sönke Rudnik, Saskia Heybrock, Etienne Coyaud, Zizhen Xu, Dante Neculai, Brian Raught, Viola Oorschot, Cecilia Heus, Judith Klumperman, Paul Saftig

{"title":"The lysosomal lipid transporter LIMP-2 is part of lysosome-ER STARD3-VAPB-dependent contact sites.","authors":"Sönke Rudnik, Saskia Heybrock, Etienne Coyaud, Zizhen Xu, Dante Neculai, Brian Raught, Viola Oorschot, Cecilia Heus, Judith Klumperman, Paul Saftig","doi":"10.1242/jcs.261810","DOIUrl":"10.1242/jcs.261810","url":null,"abstract":"LIMP-2 (also known as SCARB2) is an abundant lysosomal membrane protein. Previous studies have shown that LIMP-2 functions as a virus receptor, a chaperone for lysosomal enzyme targeting and a lipid transporter. The large luminal domain of LIMP-2 contains a hydrophobic tunnel that enables transport of phospholipids, sphingosine and cholesterol from the lysosomal lumen to the membrane. The question about the fate of the lipids after LIMP-2-mediated transport is largely unexplored. To elucidate whether LIMP-2 is present at contact sites between lysosomes and the endoplasmic reticulum (ER), we performed a proximity-based interaction screen. This revealed that LIMP-2 interacts with the endosomal protein STARD3 and the ER-resident protein VAPB. Using imaging and co-immunoprecipitation, we demonstrated colocalization and physical interaction between LIMP-2 and these proteins. Moreover, we found that interaction of LIMP-2 with VAPB required the presence of STARD3. Our findings suggest that LIMP-2 is present at ER-lysosome contact sites, possibly facilitating cholesterol transport from the lysosomal to the ER membrane. This suggests a novel mechanism for inter-organelle communication and lipid trafficking mediated by LIMP-2.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIV-1 N-myristoylation-dependent hijacking of late endosomes/lysosomes to drive Gag assembly in macrophages. HIV-1 N-肉豆蔻酰化依赖性劫持晚期内体/溶酶体，以驱动巨噬细胞中 Gag 的组装。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-15 Epub Date: 2024-11-21 DOI: 10.1242/jcs.263588

Gabriel I Guajardo-Contreras, Ana L Abdalla, Alex Chen, Meijuan Niu, Erwan Beauchamp, Luc G Berthiaume, Alan W Cochrane, Andrew J Mouland

{"title":"HIV-1 N-myristoylation-dependent hijacking of late endosomes/lysosomes to drive Gag assembly in macrophages.","authors":"Gabriel I Guajardo-Contreras, Ana L Abdalla, Alex Chen, Meijuan Niu, Erwan Beauchamp, Luc G Berthiaume, Alan W Cochrane, Andrew J Mouland","doi":"10.1242/jcs.263588","DOIUrl":"10.1242/jcs.263588","url":null,"abstract":"Macrophages represent an important viral reservoir in HIV-1-infected individuals. Different from T cells, HIV-1 assembly in macrophages occurs at intracellular compartments termed virus-containing compartments (VCCs). Our previous research in HeLa cells - in which assembly resembles that found in infected T cells - suggested that late endosomes/lysosomes (LELs) play a role in HIV-1 trafficking towards its assembly sites. However, the role of LELs during assembly at VCCs is not fully understood. Herein, we used the HIV-1-inducible cell line THP-1 GagZip as a model to study HIV-1 Gag intracellular trafficking and assembly in macrophages. We demonstrated LEL involvement at VCCs using various microscopy techniques and biochemical approaches. Live-cell imaging revealed that HIV-1 repositions LELs towards the plasma membrane and modulates their motility. We showed that Arl8b-mediated LEL repositioning is not responsible for Gag trafficking to VCCs. Additionally, the inhibition of myristoylation by PCLX-001 decreased the presence of Gag on endosomes and inhibited VCC formation in both the THP-1 cell line and primary macrophages. In conclusion, we present evidence supporting the idea that HIV-1 manipulates the LEL trajectory to guide Gag to VCCs in an N-myristoylation-dependent manner.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CENP-C-targeted PLK-1 regulates kinetochore function in C. elegans embryos. CENP-C靶向PLK-1调控优雅子胚胎中的动点功能

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-15 Epub Date: 2024-11-28 DOI: 10.1242/jcs.262327

Laura Bel Borja, Samuel J P Taylor, Flavie Soubigou, Federico Pelisch

{"title":"CENP-C-targeted PLK-1 regulates kinetochore function in C. elegans embryos.","authors":"Laura Bel Borja, Samuel J P Taylor, Flavie Soubigou, Federico Pelisch","doi":"10.1242/jcs.262327","DOIUrl":"10.1242/jcs.262327","url":null,"abstract":"Polo-like kinase 1 (PLK-1) is present in centrosomes, the nuclear envelope and kinetochores and plays a significant role in meiosis and mitosis. PLK-1 depletion or inhibition has severe consequences for spindle assembly, spindle assembly checkpoint (SAC) activation, chromosome segregation and cytokinesis. BUB-1 targets PLK-1 to the outer kinetochore and, in mammals, the inner kinetochore PLK1 targeting is mediated by the constitutive centromere associated network (CCAN). BUB-1-targeted PLK-1 plays a key role in SAC activation and has a SAC-independent role through targeting CDC-20. In contrast, whether there is a specific, non-redundant role for inner kinetochore targeted PLK-1 is unknown. Here, we used the Caenorhabditis elegans embryo to study the role of inner kinetochore PLK-1. We found that CENP-C, the sole CCAN component in C. elegans and other species, targets PLK-1 to the inner kinetochore during prometaphase and metaphase. Disruption of the CENP-C-PLK-1 interaction leads to an imbalance in kinetochore components and a defect in chromosome congression, without affecting CDC-20 recruitment. These findings indicate that PLK-1 kinetochore recruitment by CENP-C has at least partially distinct functions from outer kinetochore PLK-1, providing a platform for a better understanding of the different roles played by PLK-1 during mitosis.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11634037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic remodelling of the endoplasmic reticulum for mitosis. 有丝分裂过程中内质网的动态重塑

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-15 Epub Date: 2024-11-25 DOI: 10.1242/jcs.261444

Suzan Kors, Anne-Lore Schlaitz

引用次数: 0