Journal of cell science最新文献_第8页

Announcing the JCS-David Stephens Prize and the 2024 winner Anja Konietzny. 宣布JCS-David Stephens奖和2024年获奖者Anja Konietzny。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-04-01 Epub Date: 2025-04-04 DOI: 10.1242/jcs.263973

Michael Way

引用次数: 0

Regulation of cell migration by extracellular matrix mechanics at a glance. 细胞外基质力学对细胞迁移的调控。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-04-01 Epub Date: 2025-04-04 DOI: 10.1242/jcs.263574

Cole Allan, Ovijit Chaudhuri

{"title":"Regulation of cell migration by extracellular matrix mechanics at a glance.","authors":"Cole Allan, Ovijit Chaudhuri","doi":"10.1242/jcs.263574","DOIUrl":"10.1242/jcs.263574","url":null,"abstract":"Cell migration occurs throughout development, tissue homeostasis and regeneration, as well as in diseases such as cancer. Cells migrate along two-dimensional (2D) surfaces or interfaces, within microtracks, or in confining three-dimensional (3D) extracellular matrices. Although the basic mechanisms of 2D migration are known, recent studies have elucidated unexpected migration behaviors associated with more complex substrates and have provided insights into their underlying molecular mechanisms. Studies using engineered biomaterials for 3D culture and microfabricated channels to replicate cell confinement observed in vivo have revealed distinct modes of migration. Across these contexts, the mechanical features of the surrounding microenvironment have emerged as major regulators of migration. In this Cell Science at a Glance article and the accompanying poster, we describe physiological contexts wherein 2D and 3D cell migration are essential, report how mechanical properties of the microenvironment regulate individual and collective cell migration, and review the mechanisms mediating these diverse modes of cell migration.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"138 7","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11993253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remembering David Stephens (1971-2024). 缅怀戴维-斯蒂芬斯（1971-2024）。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-04-01 Epub Date: 2025-04-04 DOI: 10.1242/jcs.263972

Jon Lane, George Banting

引用次数: 0

Formin 3 stabilizes the cytoskeleton of Drosophila tendon cells, thus enabling them to resist muscle tensile forces. Formin 3稳定果蝇肌腱细胞的细胞骨架，从而使它们能够抵抗肌肉张力。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-04-01 Epub Date: 2025-04-11 DOI: 10.1242/jcs.263543

Helena Pissarek, Na Huang, Leanna H Frasch, Hermann Aberle, Manfred Frasch

{"title":"Formin 3 stabilizes the cytoskeleton of Drosophila tendon cells, thus enabling them to resist muscle tensile forces.","authors":"Helena Pissarek, Na Huang, Leanna H Frasch, Hermann Aberle, Manfred Frasch","doi":"10.1242/jcs.263543","DOIUrl":"10.1242/jcs.263543","url":null,"abstract":"The cytoskeleton of Drosophila tendon cells features specialized F-actin and microtubule arrays that endow these cells with resistance to the tensile forces exerted by the attached muscles. In a forward genetic screen for mutants with neuromuscular junction and muscle morphology phenotypes in larvae, we identified formin 3 (form3) as a crucial component for stabilizing these cytoskeletal arrays under muscle tension. form3 mutants exhibit severely stretched tendon cells in contact with directly attached larval body wall muscles, leading to muscle retraction and rounding. Both the actomyosin and microtubule arrays are expanded likewise in these mutants and can separate laterally in extreme cases. Analysis of a natively HA-tagged, functional version of Form3 reveals that Form3 is distributed along the length of these cytoskeletal arrays. Based on our findings and existing data on vertebrate and Caenorhabditis elegans orthologs of form3, we propose that the primary function of Form3 in this context is to co-bundle actin filaments and microtubules, thus maximizing the rigidity of these cytoskeletal structures against muscle tensile forces.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphorylation of the Aly3 C-terminus impedes aberrant endocytosis of Schizosaccharomyces pombe hexose transporter Ght5. Aly3 c末端的磷酸化阻碍了分裂糖菌通过己糖转运体Ght5的异常内吞作用。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-04-01 Epub Date: 2025-04-15 DOI: 10.1242/jcs.263572

Yusuke Toyoda, Fumie Masuda, Shigeaki Saitoh

{"title":"Phosphorylation of the Aly3 C-terminus impedes aberrant endocytosis of Schizosaccharomyces pombe hexose transporter Ght5.","authors":"Yusuke Toyoda, Fumie Masuda, Shigeaki Saitoh","doi":"10.1242/jcs.263572","DOIUrl":"10.1242/jcs.263572","url":null,"abstract":"In the fission yeast Schizosaccharomyces pombe, transcriptional upregulation and cell-surface localization of the hexose transporter Ght5 are required for cell proliferation in low glucose. As the target of rapamycin complex 2 (TORC2) signaling pathway inhibits the α-arrestin Aly3-dependent endocytosis of Ght5, we hypothesized that phosphorylation inhibits this endocytosis. To identify phosphorylation sites required for proliferation in low glucose, putatively phosphorylated serine/threonine residues of Aly3 and Ght5 were replaced with alanine, showing that C-terminal serine residues of Aly3, but not Ght5, are necessary for proliferation in low glucose. Expression of Aly3 that could not be phosphorylated at the C-terminus led to increased ubiquitylation and vacuolar accumulation of Ght5 in low glucose, but reversion of one of the alanine residues to serine reversed those defects. Also, Aly3 physically interacted with the HECT-type ubiquitin ligases Pub1 and Pub3, and these interactions were required for surface localization of Ght5 and proliferation in low glucose. This study reveals the mechanisms by which Aly3 is regulated so that fission yeast can adapt to nutritional stress.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rab40 GTPases regulate AMBRA1-mediated transcription and cell migration. Rab40 GTPases调节ambra1介导的转录和细胞迁移。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-04-01 Epub Date: 2025-04-11 DOI: 10.1242/jcs.263707

Revathi Sampath, Katherine Vaeth, Valeryia Mikalayeva, Vytenis Arvydas Skeberdis, Rytis Prekeris, Ke-Jun Han

{"title":"Rab40 GTPases regulate AMBRA1-mediated transcription and cell migration.","authors":"Revathi Sampath, Katherine Vaeth, Valeryia Mikalayeva, Vytenis Arvydas Skeberdis, Rytis Prekeris, Ke-Jun Han","doi":"10.1242/jcs.263707","DOIUrl":"10.1242/jcs.263707","url":null,"abstract":"The Rab40 subfamily of proteins consists of unique small monomeric GTPases that form CRL5-based ubiquitin E3 ligase complexes and regulate ubiquitylation of specific target proteins. Recent studies have shown that Rab40 proteins play an important role in regulating cell migration, but the underlying mechanisms of how the Rab40-CRL5 complex functions are still not fully understood. In this study, we identified AMBRA1 as a novel binding partner of Rab40 GTPases and show that this interaction mediates a bidirectional crosstalk between the CRL4 and CRL5 E3 ligases. Importantly, we found that Rab40-CRL5 ubiquitylates AMBRA1, which does not result in AMBRA1 degradation but, instead, appears to induce AMBRA1-dependent regulation of gene transcription. The global transcriptional profiles identified by RNA sequencing showed that AMBRA1 regulates transcription of genes related to cell adhesion and migration. Additionally, we show that AMBRA1-dependent transcription regulation does not require the enzymatic activity of AMBRA1-CRL4, and that Rab40-induced AMBRA1 ubiquitylation leads to dissociation of the AMBRA1-CRL4 complex. Taken together, our findings reveal a novel function of the Rab40-CRL5 complex as an important regulator of AMBRA1-dependent transcription of genes involved in cell migration.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Practical considerations for data exploration in quantitative cell biology. 定量细胞生物学中数据探索的实际考虑。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-04-01 Epub Date: 2025-04-07 DOI: 10.1242/jcs.263801

Joanna W Pylvänäinen, Hanna Grobe, Guillaume Jacquemet

引用次数: 0

Exploiting the SunTag system to study the developmental regulation of mRNA translation. 利用SunTag系统研究mRNA翻译的发育调控。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-03-15 Epub Date: 2025-03-27 DOI: 10.1242/jcs.263457

Alastair Pizzey, Catherine Sutcliffe, Jennifer C Love, Emmanuel Akabuogu, Magnus Rattray, Mark P Ashe, Hilary L Ashe

{"title":"Exploiting the SunTag system to study the developmental regulation of mRNA translation.","authors":"Alastair Pizzey, Catherine Sutcliffe, Jennifer C Love, Emmanuel Akabuogu, Magnus Rattray, Mark P Ashe, Hilary L Ashe","doi":"10.1242/jcs.263457","DOIUrl":"10.1242/jcs.263457","url":null,"abstract":"The ability to quantitatively study mRNA translation using SunTag imaging is transforming our understanding of the translation process. Here, we expand the SunTag method to study new aspects of translation regulation in Drosophila. Repression of the maternal hunchback (hb) mRNA in the posterior of the Drosophila embryo is a textbook example of translational control. Using SunTag imaging to quantify translation of maternal SunTag-hb mRNAs, we show that repression in the posterior is leaky, as ∼5% of SunTag-hb mRNAs are translated. In the anterior of the embryo, the maternal and zygotic SunTag-hb mRNAs show similar translation efficiency despite having different untranslated regions (UTRs). We demonstrate that the SunTag-hb mRNA can be used as a reporter to study ribosome pausing at single-mRNA resolution, by exploiting the conserved xbp1 mRNA and A60 pausing sequences. Finally, we adapt the detector component of the SunTag system to visualise and quantify translation of the short gastrulation (sog) mRNA, encoding an essential secreted extracellular BMP regulator, at the endoplasmic reticulum in fixed and live embryos. Together, these tools will facilitate the future dissection of translation regulatory mechanisms during development.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct neutrophil and T cell contact with macrophages induces release of phagosomally processed PAMPs via eructophagy. 中性粒细胞和T细胞与巨噬细胞的直接接触诱导巨噬细胞通过噬噬释放吞噬处理的PAMPs。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-03-15 Epub Date: 2025-03-26 DOI: 10.1242/jcs.263731

Jenny A Nguyen, Tanis L Orsetti, Philip Vernon, Catherine J Greene, Neil McKenna, Robin M Yates

{"title":"Direct neutrophil and T cell contact with macrophages induces release of phagosomally processed PAMPs via eructophagy.","authors":"Jenny A Nguyen, Tanis L Orsetti, Philip Vernon, Catherine J Greene, Neil McKenna, Robin M Yates","doi":"10.1242/jcs.263731","DOIUrl":"10.1242/jcs.263731","url":null,"abstract":"Macrophages play a pivotal role in clearing debris and microbes from the microenvironment via phagocytosis and orchestrating local inflammation. Although pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) are understood to mostly be released through the synthesis and secretion of soluble mediators, such as cytokines and eicosanoids, it has been recently proposed that macrophages can release previously phagocytosed and processed PAMPs and DAMPs into the local microenvironment via a process termed eructophagy, and that these, in turn, can activate recently recruited leukocytes. Additionally, it has been commonly observed that local macrophages physically interact with other leukocytes, such as neutrophils and T cells, recruited to sites of inflammation. This study demonstrates that eructophagy in macrophages is significantly induced during physical interaction with neutrophils and T cells, which is mediated by ICAM1 on macrophages and lymphocyte function-associated antigen 1 (LFA1) on neutrophils and T cells. Notably, ICAM1 activation alone is sufficient to trigger eructophagy in macrophages and is dependent on Lyn kinase. Through this mechanism, it is proposed that neutrophils and lymphocytes can influence their own activation by interacting with local macrophages containing PAMP-containing phagolysosomes, which subsequently triggers PAMP release into the local microenvironment through eructophagy.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fibronectin matrix assembly at a glance. 纤维连接蛋白矩阵组装一目了然。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-03-15 Epub Date: 2025-03-25 DOI: 10.1242/jcs.263834

Yu Sun, Aaron J Hamlin, Jean E Schwarzbauer

引用次数: 0