Journal of cell science最新文献_第7页

Regulation of miR-206 in denervated and dystrophic muscles, and its effect on acetylcholine receptor clustering. 变性肌肉和肌营养不良肌肉中 MiR-206 的调控及其对 AChR 聚类的影响

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-15 Epub Date: 2024-12-13 DOI: 10.1242/jcs.262303

Joseph Barden, Olivia Kosloski, Amir Jadidian, Mohammed Akaaboune

{"title":"Regulation of miR-206 in denervated and dystrophic muscles, and its effect on acetylcholine receptor clustering.","authors":"Joseph Barden, Olivia Kosloski, Amir Jadidian, Mohammed Akaaboune","doi":"10.1242/jcs.262303","DOIUrl":"10.1242/jcs.262303","url":null,"abstract":"The muscle-specific microRNA miR-206 has recently emerged as a potential regulator of genes involved in the formation and regeneration of the neuromuscular junction (NMJ). This study investigated miR-206-3p (miR-206) expression in synaptic and non-synaptic regions of denervated mice and α-dystrobrevin (Dtna)-knockout mice, as well as its impact on the formation and/or maintenance of agrin-induced acetylcholine receptor (AChR) clusters. In denervated, Dtna-deficient and crushed muscles, miR-206 expression significantly increased compared to what was seen for innervated muscles. Although miR-206 expression was slightly elevated in the synaptic regions of innervated muscles, it was dramatically increased in non-synaptic areas of denervated muscles. miR-206 targets transcripts of essential NMJ proteins, such as Dtna, α-syntrophin (Snta1) and rapsyn, but not the AChRα subunit (encoded by Chrna1) or Lrp4 in innervated muscles. However, in denervated muscles, AChRα transcripts, which increased significantly, become a target of miR-206. Co-expression of miR-206 with rapsyn, Dtna and Snta1 in C2C12 myoblasts significantly reduced their protein levels, and overexpression of miR-206 in myotubes disrupted agrin-induced AChR clustering. These results indicate that miR-206 fine-tunes NMJ signaling proteins by regulating transcripts of various proteins with different localizations under normal and pathological conditions.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11795291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phase separation of microtubule-binding proteins - implications for neuronal function and disease. 微管结合蛋白的相分离--对神经元功能和疾病的影响

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-15 Epub Date: 2024-12-13 DOI: 10.1242/jcs.263470

Daisy Duan, Anthony J Koleske

引用次数: 0

Correction: Complexity and self-organization in the evolution of cell polarization. 修正：细胞极化进化中的复杂性和自组织。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-11 DOI: 10.1242/jcs.263699

Marieke M Glazenburg, Liedewij Laan

引用次数: 0

Extracellular vesicles and nanoparticles at a glance. 细胞外囊泡和纳米颗粒一览。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-06 DOI: 10.1242/jcs.260201

Dennis K Jeppesen, Qin Zhang, Robert J Coffey

引用次数: 0

An image-based RNAi screen identifies the EGFR signaling pathway as a regulator of Imp RNP granules. 基于图像的 RNAi 筛选确定 EGF.R 信号通路是 Imp/ IGF2BP RNP 颗粒的调节因子。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-10 DOI: 10.1242/jcs.262119

Fabienne De Graeve, Eric Debreuve, Kavya Vinayan Pushpalatha, Xuchun Zhang, Somia Rahmoun, Djampa Kozlowski, Nicolas Cedilnik, Jeshlee Vijayakumar, Paul Cassini, Sebastien Schaub, Xavier Descombes, Florence Besse

{"title":"An image-based RNAi screen identifies the EGFR signaling pathway as a regulator of Imp RNP granules.","authors":"Fabienne De Graeve, Eric Debreuve, Kavya Vinayan Pushpalatha, Xuchun Zhang, Somia Rahmoun, Djampa Kozlowski, Nicolas Cedilnik, Jeshlee Vijayakumar, Paul Cassini, Sebastien Schaub, Xavier Descombes, Florence Besse","doi":"10.1242/jcs.262119","DOIUrl":"10.1242/jcs.262119","url":null,"abstract":"Biomolecular condensates have recently retained much attention given that they provide a fundamental mechanism of cellular organization. Among those, cytoplasmic ribonucleoprotein (RNP) granules selectively and reversibly concentrate RNA molecules and regulatory proteins, thus contributing to the spatiotemporal regulation of associated RNAs. Extensive in vitro work has unraveled the molecular and chemical bases of RNP granule assembly. The signaling pathways controlling this process in a cellular context are, however, still largely unknown. Here, we aimed at identifying regulators of cytoplasmic RNP granules characterized by the presence of the evolutionarily conserved Imp RNA-binding protein (a homolog of IGF2BP proteins). We performed a high-content image-based RNAi screen targeting all Drosophila genes encoding RNA-binding proteins, phosphatases and kinases. This led to the identification of dozens of genes regulating the number of Imp-positive RNP granules in S2R+ cells, among which were components of the MAPK pathway. Combining functional approaches, phospho-mapping and generation of phospho-variants, we further showed that EGFR signaling inhibits Imp-positive RNP granule assembly through activation of the MAPK-ERK pathway and downstream phosphorylation of Imp at the S15 residue. This work illustrates how signaling pathways can regulate cellular condensate assembly by post-translational modifications of specific components.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11698055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dual Ras-association domains of Drosophila Canoe have differential roles in linking cell junctions to the cytoskeleton during morphogenesis. 果蝇 Canoe 的双 Ras 关联（RA）域在形态发生过程中连接细胞连接和细胞骨架方面具有不同的作用。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-11 DOI: 10.1242/jcs.263546

Emily D McParland, Noah J Gurley, Leah R Wolfsberg, T Amber Butcher, Abhi Bhattarai, Corbin C Jensen, Ruth I Johnson, Kevin C Slep, Mark Peifer

{"title":"The dual Ras-association domains of Drosophila Canoe have differential roles in linking cell junctions to the cytoskeleton during morphogenesis.","authors":"Emily D McParland, Noah J Gurley, Leah R Wolfsberg, T Amber Butcher, Abhi Bhattarai, Corbin C Jensen, Ruth I Johnson, Kevin C Slep, Mark Peifer","doi":"10.1242/jcs.263546","DOIUrl":"10.1242/jcs.263546","url":null,"abstract":"During development cells must change shape and move without disrupting dynamic tissue architecture. This requires robust linkage of cell-cell adherens junctions to the force-generating actomyosin cytoskeleton. Drosophila Canoe and mammalian afadin play key roles in the regulation of such linkages. One central task for the field is defining mechanisms by which upstream inputs from Ras-family GTPases regulate Canoe and afadin. These proteins are unusual in sharing two tandem Ras-association (RA) domains - RA1 and RA2 - which when deleted virtually eliminate Canoe function. Work in vitro has suggested that RA1 and RA2 differ in GTPase affinity, but their individual functions in vivo remain unknown. Combining bioinformatic and biochemical approaches, we find that both RA1 and RA2 bind to active Rap1 with similar affinities, and that their conserved N-terminal extensions enhance binding. We created Drosophila canoe mutants to test RA1 and RA2 function in vivo. Despite their similar affinities for Rap1, RA1 and RA2 play strikingly different roles. Deleting RA1 virtually eliminates Canoe function, whereas mutants lacking RA2 are viable and fertile but have defects in junctional reinforcement in embryos and during pupal eye development. These data significantly expand our understanding of the regulation of adherens junction-cytoskeletal linkages.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11698047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topology surveillance of the lanosterol demethylase CYP51A1 by signal peptide peptidase. 信号肽肽酶对羊毛甾醇脱甲基酶 CYP51A1 的拓扑监测

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-12 DOI: 10.1242/jcs.262333

Nikita Sergejevs, Dönem Avci, Michael L van de Weijer, Robin A Corey, Marius K Lemberg, Pedro Carvalho

引用次数: 0

The small ARF-like 2 GTPase TITAN5 is linked with the dynamic regulation of IRON-REGULATED TRANSPORTER 1. 小型类 ARF 2 GTPase TITAN 5 与铁调节运输器 1 的动态调控有关。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-10 DOI: 10.1242/jcs.263645

Inga Mohr, Monique Eutebach, Marie C Knopf, Naima Schommen, Regina Gratz, Kalina Angrand, Lara Genders, Tzvetina Brumbarova, Petra Bauer, Rumen Ivanov

{"title":"The small ARF-like 2 GTPase TITAN5 is linked with the dynamic regulation of IRON-REGULATED TRANSPORTER 1.","authors":"Inga Mohr, Monique Eutebach, Marie C Knopf, Naima Schommen, Regina Gratz, Kalina Angrand, Lara Genders, Tzvetina Brumbarova, Petra Bauer, Rumen Ivanov","doi":"10.1242/jcs.263645","DOIUrl":"10.1242/jcs.263645","url":null,"abstract":"Iron acquisition is crucial for plants. The abundance of IRON-REGULATED TRANSPORTER 1 (IRT1) is controlled through endomembrane trafficking, a process that requires small ARF-like GTPases. Only few components that are involved in the vesicular trafficking of specific cargo are known. Here, we report that the ARF-like GTPase TITAN5 (TTN5) interacts with the large cytoplasmic variable region and protein-regulatory platform of IRT1. Heterozygous ttn5-1 plants can display reduced root iron reductase activity. This activity is needed for iron uptake via IRT1. Fluorescent fusion proteins of TTN5 and IRT1 colocalize at locations where IRT1 sorting and cycling between the plasma membrane and the vacuole are coordinated. TTN5 can also interact with peripheral membrane proteins that are components of the IRT1 regulation machinery, like the trafficking factor SNX1, the C2 domain protein EHB1 and the SEC14-GOLD protein PATL2. Hence, the link between iron acquisition and vesicular trafficking involving a small GTPase of the ARF family opens up the possibility to study the involvement of TTN5 in nutritional cell biology and the endomembrane system.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CAMSAP3 forms dimers via its α-helix domain that directly stabilize non-centrosomal microtubule minus ends. CAMSAP3 通过其 α-helix 结构域形成二聚体，直接稳定非中心粒微管负端。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-09 DOI: 10.1242/jcs.263609

Yuejia Li, Rui Zhang, Jinqi Ren, Wei Chen, Zhengrong Zhou, Honglin Xu, Dong Li, Haisu Cheng, Qi Xie, Wei Ji, Wei Feng, Xin Liang, Wenxiang Meng

{"title":"CAMSAP3 forms dimers via its α-helix domain that directly stabilize non-centrosomal microtubule minus ends.","authors":"Yuejia Li, Rui Zhang, Jinqi Ren, Wei Chen, Zhengrong Zhou, Honglin Xu, Dong Li, Haisu Cheng, Qi Xie, Wei Ji, Wei Feng, Xin Liang, Wenxiang Meng","doi":"10.1242/jcs.263609","DOIUrl":"10.1242/jcs.263609","url":null,"abstract":"Microtubules are vital components of the cytoskeleton. Their plus ends are dynamic and respond to changes in cell morphology, whereas the minus ends are stable and serve a crucial role in microtubule seeding and maintaining spatial organization. In mammalian cells, the calmodulin-regulated spectrin-associated proteins (CAMSAPs), play a key role in directly regulating the dynamics of non-centrosomal microtubules minus ends. However, the molecular mechanisms are not yet fully understood. Our study reveals that CAMSAP3 forms dimers through its C-terminal α-helix; this dimerization not only enhances the microtubule-binding affinity of the CKK domain but also enables the CKK domain to regulate the dynamics of microtubules. Furthermore, CAMSAP3 also specializes in decorating at the minus end of microtubules through the combined action of the microtubule-binding domain (MBD) and the C-terminal α-helix, thereby achieving dynamic regulation of the minus ends of microtubules. These findings are crucial for advancing our understanding and treatment of diseases associated with non-centrosomal microtubules.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Borg5 restricts contractility and motility in epithelial MDCK cells. Borg5/Cdc42EP1 限制了上皮 MDCK 细胞的收缩性和运动性。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-10 DOI: 10.1242/jcs.261705

David Cohen, Dawn Fernandez, Francisco Lázaro-Diéguez, Beatrix Überheide, Anne Müsch

{"title":"Borg5 restricts contractility and motility in epithelial MDCK cells.","authors":"David Cohen, Dawn Fernandez, Francisco Lázaro-Diéguez, Beatrix Überheide, Anne Müsch","doi":"10.1242/jcs.261705","DOIUrl":"10.1242/jcs.261705","url":null,"abstract":"The Borg (or Cdc42EP) family consists of septin-binding proteins that are known to promote septin-dependent stress fibers and acto-myosin contractility. We show here that epithelial Borg5 (also known as Cdc42EP1) instead limits contractility, cell-cell adhesion tension and motility, as is required for the acquisition of columnar, isotropic cell morphology in mature MDCK monolayers. Borg5 depletion inhibited the development of the lateral F-actin cortex and stimulated microtubule-dependent leading-edge lamellae as well as radial stress fibers and, independently of the basal F-actin phenotype, caused anisotropy of apical surfaces within compacted monolayers. We determined that Borg5 limits colocalization of septin proteins with microtubules, and that like septin 2, Borg5 interacts with the rod-domain of myosin IIA (herein referring to the MYH9 heavy chain). The interaction of myosin IIA with Borg5 was reduced in the presence of septins. Because septins also mediate myosin activation, we propose that Borg5 limits contractility in MDCK cells in part by counteracting septin-associated myosin activity.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11698036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0