Journal of cellular biochemistry最新文献_第2页

Panoptosis Targets the Mechanism of PRDX1 Involvement in Acute Myocardial Infarction via ZBP1

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-03-20 DOI: 10.1002/jcb.70026

Yu Sun, Chengcheng Ji, Yuanmei Zhang, Shuai Liu, Yin Ye, Tian Li, Shijia Wu, Tianfu Sun, Hao Yin, Wen Yan, Wen Jin

{"title":"Panoptosis Targets the Mechanism of PRDX1 Involvement in Acute Myocardial Infarction via ZBP1","authors":"Yu Sun, Chengcheng Ji, Yuanmei Zhang, Shuai Liu, Yin Ye, Tian Li, Shijia Wu, Tianfu Sun, Hao Yin, Wen Yan, Wen Jin","doi":"10.1002/jcb.70026","DOIUrl":"10.1002/jcb.70026","url":null,"abstract":"<div>\u0000 \u0000 <p>PANoptosis is involved in various pathological processes, but its role in acute myocardial infarction (AMI) remains unclear. This study aimed to explore the mechanism by which PANoptosis is involved in AMI. GSE172270 was used as an internal test set, and GSE159657 served as an external validation set to identify disease targets for AMI. WGCNA was performed to identify potential hub genes associated with AMI. Then, genes related to PANoptosis among the disease targets of AMI were screened. Additionally, human cardiomyocytes AC16 and mouse cardiomyocytes HL-1 were cultured in sugar-free and serum-free medium for 4 h and 12 h, and the expression of key genes regulating PANoptosis was detected by qRT-PCR. Finally, mRNA interference and overexpression experiments were conducted to verify the expression of key mRNAs. A total of 45 upregulated and 125 downregulated differentially expressed genes (DEGs) were identified in the GSE172270 data set. WGCNA identified 891 potential hub genes associated with AMI. In the GSE159657 data set, 695 upregulated and 552 downregulated DEGs were identified. Four genes related to AMI-PANoptosis (PRDX1, MMACHC, BLVRB, and TXNL1) were screened. Through qRT-PCR verification, PRDX1 was identified as the most specific and significant gene. The expression of the PANoptosis positive regulator ZPB1 was upregulated, while the PANoptosis negative regulator TAK1 was downregulated. Additionally, key cell death genes (MLKL, p-MLKL, Caspase-3, Caspase-7, and GSDMD) were upregulated in sugar-free and serum-free culture. However, PRDX1 interference reversed these effects. Our study demonstrated that PRDX1 is a key regulator of PANoptosis in AMI.</p></div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 3","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Melatonin on the Expression of Invasion-Related Markers (MMP2 and MMP9) in Breast Cancer Cells

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-03-16 DOI: 10.1002/jcb.70010

Parvin Ghadimi, Saeid Ghorbian

{"title":"Effects of Melatonin on the Expression of Invasion-Related Markers (MMP2 and MMP9) in Breast Cancer Cells","authors":"Parvin Ghadimi, Saeid Ghorbian","doi":"10.1002/jcb.70010","DOIUrl":"https://doi.org/10.1002/jcb.70010","url":null,"abstract":"<div>\u0000 \u0000 <p>Breast cancer is one of the most common types of cancer in women, and metastasis is a leading cause of mortality in patients with this disease. This study investigated the effects of melatonin, a natural hormone, on the migration of cancer cells in two cell lines, MCF-7 and MDA-MB-231. MCF-7 and MDA-MB-231 cells were cultured in their respective media. The effective dose of melatonin in each cell line was determined using the MTT assay. The effects of IC50 melatonin on cell migration were assessed using the wound-healing assay. The expression of the invasion-related genes (MMP2 and MMP9), as well as the melatonin receptors MT1 and MT2, was analyzed using Real-Time RT-PCR. The wound-healing assay results indicated that 48 h of melatonin treatment at doses of 2.5 and 3.5 M significantly reduced migration in MCF-7 and MDA-MB-231 cells. In addition, melatonin treatment decreased the invasion-related markers of both cell lines. Melatonin also increased the expression of MT1 and MT2 receptors in both cell lines, and the expression of MMP2 and MMP9 was significantly reduced by melatonin (<i>p</i> < 0.05). Our results indicate that melatonin, a naturally occurring compound, possesses the potential to inhibit the movement and spread of breast cancer cells by elevating the levels of MT1 and MT2 receptors, resulting in a reduction of matrix metalloproteinases 2 and 9 expression.</p>\u0000 </div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 3","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platelet-Derived Growth Factor Promotes Glomerular Mesangial Cells Differentiation of Human Bone Marrow Hematopoietic Stem Cells - An In Vitro Study

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-03-11 DOI: 10.1002/jcb.70012

Surekha Kattaru, Samundeshwari Echambadi Loganathan, Sireesha Kodavala, Chandra Sekhar Chodimella, Venkata Gurunadha Krishna Sarma Potukuchi

{"title":"Platelet-Derived Growth Factor Promotes Glomerular Mesangial Cells Differentiation of Human Bone Marrow Hematopoietic Stem Cells - An In Vitro Study","authors":"Surekha Kattaru, Samundeshwari Echambadi Loganathan, Sireesha Kodavala, Chandra Sekhar Chodimella, Venkata Gurunadha Krishna Sarma Potukuchi","doi":"10.1002/jcb.70012","DOIUrl":"https://doi.org/10.1002/jcb.70012","url":null,"abstract":"<div>\u0000 \u0000 <p>Glomerular filtration function and homeostasis are largely due to the cross-talk between podocytes, endothelial cells, and mesangial cells (MCs). Any disturbance in this association causes glomerular diseases (GD). Cell-based therapies are the best option in the treatment of GD. It is contemplated that hematopoietic stem cells (HSCs) are best suited to regenerate these cells; earlier, we have shown the differentiation of HSCs into podocytes. In this study, MCs formation was initiated with retinoic acid (RA), BMP-7, and Activin A, resulting in comma-shaped intermediate mesoderm (IM) cells prominently expressing Osr1. Followed by inducing with EGF, FGF, and BMP-7, which resulted in elongated metanephric mesenchyme (MM) cells conspicuously expressing Pax2, Wt1, Foxd1, and Eya1. Finally, MM cells were induced with platelet-derived growth factor to form polygonal-shaped MCs expressing α-smooth muscle actin, desmin, CD44, and PDGFRβ. The growing MCs showed positivity to periodic acid Schiff's, and ANAE staining with a prominent expression of the Itga8 elucidating phagocytic property of MCs. These MCs showed conspicuous expression of CD133, notch-2, and telomerase, determining the quiescence nature with a 31.2% proliferation rate revealed through Ki-67 staining. The functionality of MCs was assessed by growing MCs in 5.5 and 25 mM glucose concentrations, and noticeable expression of angiotensinogen, angiotensin-I and II, angiotensin-converting enzyme, collagen-4, fibronectin, and TGFβ1 was observed in 25 mM concentration, while lowered expression of these genes was observed in 5.5 mM concentration explaining the role of MCs in regulating the filtration pressure. All these findings demonstrate that HSCs can rejuvenate the insulted glomerulus.</p>\u0000 </div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 3","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Evaluation of IGF1R and Its Associated Proteins as Targets and Design of Novel Inhibitors for Cancer Therapy

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-03-02 DOI: 10.1002/jcb.70008

Tabrez Faruqui, Aubaidah Akhtar, Farheen Showket, Mohd Jamal Dar, Yusuf Akhter

{"title":"Identification and Evaluation of IGF1R and Its Associated Proteins as Targets and Design of Novel Inhibitors for Cancer Therapy","authors":"Tabrez Faruqui, Aubaidah Akhtar, Farheen Showket, Mohd Jamal Dar, Yusuf Akhter","doi":"10.1002/jcb.70008","DOIUrl":"https://doi.org/10.1002/jcb.70008","url":null,"abstract":"<div>\u0000 \u0000 <p>The insulin-like growth factor 1 receptor (IGF1R) is a crucial receptor tyrosine kinase involved in cellular growth, survival, and metabolism. Abnormal overexpression and activation are common in various cancers and contribute to tumor development and resistance to treatment. The STRING database was used to analyze the protein–protein interaction network of IGF1R and was visualized using Cytoscape to identify the key associated proteins. We assessed IGF1R and its associated protein expression levels across pan-cancer types and compared them to healthy controls using a TNMplot and cBioPortal. The objective of this study was to identify novel, low-toxicity inhibitors targeting the IGF1R and its associated proteins (e.g., AKT1 and EGFR) with better pharmacokinetic profiles for effective cancer treatment, including brain cancer. We screened 693 million drug-like compounds and selected the top 400 for toxicity analysis using ProTox-II, which identified 83 nontoxic candidates. These were categorized as either blood–brain barrier (BBB) permeant or impermeant. Molecular docking studies with AutoDock Vina 4.1 were performed on 17 target proteins, including IGF1R, with the top three compounds. Subsequently, molecular dynamics simulations using Desmond were conducted on the two most promising candidates: two BBB permeants and two impermeants. Our study identified six nontoxic IGF1R inhibitors and 16 other target protein inhibitors. Docking and MD simulations confirmed the potential of these compounds in targeted therapies. Notably, both BBB-permeant and -impermeant compounds in complex with the target proteins showed stability over 50 and 400 ns molecular simulation experiments, highlighting their potential in cancer therapy and suggesting the need for further in vitro and in vivo validation.</p></div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 3","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Overexpression of lncRNA WWTR1-AS1 Associates With Tumor Aggressiveness and Unfavorable Survival in Head–Neck Squamous Cell Carcinoma”

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-03-02 DOI: 10.1002/jcb.70004

引用次数: 0

RETRACTION: MiR-101-3p Regulates the Viability of Lung Squamous Carcinoma Cells via Targeting EZH2

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-02-25 DOI: 10.1002/jcb.70007

引用次数: 0

Exploring the Impact of LRRK2 WD40 G2294R Mutation on Conformation and Dimerisation Dynamics: Insights From Molecular Dynamics Simulation

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-02-25 DOI: 10.1002/jcb.70011

Chuancheng Wei, Choon Han Heh, Sek Peng Chin

{"title":"Exploring the Impact of LRRK2 WD40 G2294R Mutation on Conformation and Dimerisation Dynamics: Insights From Molecular Dynamics Simulation","authors":"Chuancheng Wei, Choon Han Heh, Sek Peng Chin","doi":"10.1002/jcb.70011","DOIUrl":"https://doi.org/10.1002/jcb.70011","url":null,"abstract":"<div>\u0000 \u0000 <p>LRRK2 has gained prominence in treating Parkinson's disease as a potential drug target. Mutations in the WD40 domain, like G2294R, are notable for their influence on the stability and dimerisation of the LRRK2. Studies have shown that G2294R could result in the WD40 distortion and destabilised LRRK2 protein. However, the underlying mechanism remains unclear. To elucidate how the G2294R mutation in the WD40 domain affects the structural and functional conformation of LRRK2, the structure of WD40 G2294R was constructed using homology modelling, and the molecular dynamics simulations on G2294R and wild-type dimers and monomers were carried out. The results show that distortion mainly occurs in the areas of β3, L1, β5, L2, and β7. The dimerisation was enhanced through the conformational changes in the G2294R variant, while the domains show different contributions towards the dimerisation. Our study reveals the effects of G2294R on the WD40. It explores its role in dimerisation and distortion, which could contribute to developing novel WD40 inhibitors and elucidate the molecular mechanism of WD40 dimerisation-monomerisation equilibrium.</p>\u0000 </div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding SARS-CoV-2 Inhibition: Insights From Molecular Dynamics Simulation of Condensed Amino Thiourea Scaffold Small Molecules

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-02-23 DOI: 10.1002/jcb.70005

Xiaoli Shen, Hao Zhang, Pengyin Zhang, Xuerui Zhao, Chang Liu, Jianan Ju, Aijun Liu, Song Wang

{"title":"Decoding SARS-CoV-2 Inhibition: Insights From Molecular Dynamics Simulation of Condensed Amino Thiourea Scaffold Small Molecules","authors":"Xiaoli Shen, Hao Zhang, Pengyin Zhang, Xuerui Zhao, Chang Liu, Jianan Ju, Aijun Liu, Song Wang","doi":"10.1002/jcb.70005","DOIUrl":"https://doi.org/10.1002/jcb.70005","url":null,"abstract":"<div>\u0000 \u0000 <p>The main protease (M<sup>pro</sup>) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) plays a crucial role in viral replication. In this study, the binding modes and inhibitory mechanisms of eight condensed amino thiourea scaffold inhibitors of M<sup>pro</sup> in proteins were investigated using a combination of molecular docking, molecular dynamics simulations, and MM/PBSA binding free energy calculations. The results indicated that the para-hydroxyl group on the benzene ring at the head of the inhibitor has a decisive influence on the initial docking pose and binding free energy strength of the inhibitor. Additionally, the position and length of the hydrophobic side chain on the tail six-membered ring significantly impacted the final binding pose of the inhibitor. The presence of a long hydrophobic side chain in the ortho position of this ring, through its interaction with the P4 hydrophobic pocket, led to an opposite binding mode in the protein compared with when it was present with or without the para-side chain. Different lengths of para-substituted side chains affected the positioning of the inhibitors in the enzyme. These different binding modes led to variations in the binding free energy between the inhibitor and the protein, which in turn gave rise to differences in inhibitory capability.</p></div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peroxisome Proliferator-Activated Receptor-Gamma Activation by an Active Compound in Lythrum anceps (Koehne) Makino

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-02-21 DOI: 10.1002/jcb.70009

Ryo Miyata, Masanobu Suzuki, Yuka Okazaki, Daigo Abe, Yoshihiro Nakajima

{"title":"Peroxisome Proliferator-Activated Receptor-Gamma Activation by an Active Compound in Lythrum anceps (Koehne) Makino","authors":"Ryo Miyata, Masanobu Suzuki, Yuka Okazaki, Daigo Abe, Yoshihiro Nakajima","doi":"10.1002/jcb.70009","DOIUrl":"https://doi.org/10.1002/jcb.70009","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Lythrum anceps</i> (Koehne) Makino (Japanese common name: “Misohagi”) is an edible plant belonging to the Lythraceae family. It is mainly distributed in Asia, Northern Africa, and Europe. Plants of the genus <i>Lythrum</i> exhibit a broad range of biological activities including anti-inflammatory and antimicrobial activities. Because of this, the plants are used in traditional medicine to treat hemorrhage, infected wounds, and dysentery. The activation of peroxisome proliferator-activated receptor-gamma (PPARγ) is an effective target for improving insulin resistance and anti-inflammatory activity. However, PPARγ activation by the genus <i>Lythrum</i> remains unclear. Aiming to evaluate PPARγ activation by <i>L. anceps</i>, we generated a reporter cell line using an artificial chromosome vector that stably expresses dual-color beetle luciferases. Dual-color real-time bioluminescence monitoring revealed marked PPARγ activation in <i>L. anceps</i> extracts. Moreover, ellagic acid was identified as a PPARγ activator present in <i>L. anceps</i> by a bioassay-guided fractionation approach.</p>\u0000 </div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming Growth Factor-β Modulates Cancer Stem Cell Traits on CD44 Subpopulations in Hepatocellular Carcinoma 转化生长因子-β调节肝细胞癌 CD44 亚群的癌症干细胞特征

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-02-12 DOI: 10.1002/jcb.70003

Mario Alejandro Aguilar-Chaparro, Sonia Andrea Rivera-Pineda, Hury Viridiana Hernández-Galdámez, Emmanuel Ríos-Castro, Olga Lilia Garibay-Cerdenares, Carolina Piña-Vázquez, Saúl Villa-Treviño

{"title":"Transforming Growth Factor-β Modulates Cancer Stem Cell Traits on CD44 Subpopulations in Hepatocellular Carcinoma","authors":"Mario Alejandro Aguilar-Chaparro, Sonia Andrea Rivera-Pineda, Hury Viridiana Hernández-Galdámez, Emmanuel Ríos-Castro, Olga Lilia Garibay-Cerdenares, Carolina Piña-Vázquez, Saúl Villa-Treviño","doi":"10.1002/jcb.70003","DOIUrl":"https://doi.org/10.1002/jcb.70003","url":null,"abstract":"<p>Hepatocellular carcinoma (HCC) is a formidable malignancy, with growing interest in identifying cancer stem cells (CSCs) as potential therapeutic targets. CD44 isoforms have emerged as promising CSC markers in HCC, often associated with epithelial-mesenchymal transition (EMT) induced by transforming growth factor-beta (TGF-β). However, the intricate relationship between CSC traits, CD44 isoforms, and TGF-β effects on CD44 subpopulations in HCC remains unclear. This study aimed to clarify how TGF-β influences proteomic changes and CSC traits in subpopulations expressing standard CD44 isoform (CD44std) and CD44 variant 9 (CD44v9). Treating SNU-423 cells with TGF-β lead to notable morphological changes, resembling a spindle-like phenotype, along with reductions in CD44v9+ subpopulations and differential CD44std expression. Proteomic analysis highlighted significant alterations in signaling pathways, particularly the mitogen-activated protein kinase (MAPK) pathway. Validation experiments demonstrated upregulation in CD44std cells and downregulation in CD44v9 cells post-TGF-β treatment. Furthermore, TGF-β exerted regulatory influence over Sox2 and Nanog expression, resulting in increased colony and spheroid formation in CD44std cells but decreased capabilities in CD44v9 cells. TGF-β also enhanced the migratory and invasive properties of both subpopulations through EMT, alongside increased adhesive abilities in CD44v9 cells. These findings illuminate the dynamic interplay between TGF-β and CD44std/CD44v9 subpopulations, emphasizing the role of MAPK signaling and modulation of CSC traits. This research contributes to understanding the dynamic interplay between CD44 isoforms and TGF-β in HCC.</p>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcb.70003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0