Journal of cellular biochemistry最新文献

RETRACTION: MiR-101-3p Regulates the Viability of Lung Squamous Carcinoma Cells via Targeting EZH2

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-02-25 DOI: 10.1002/jcb.70007

引用次数: 0

Exploring the Impact of LRRK2 WD40 G2294R Mutation on Conformation and Dimerisation Dynamics: Insights From Molecular Dynamics Simulation

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-02-25 DOI: 10.1002/jcb.70011

Chuancheng Wei, Choon Han Heh, Sek Peng Chin

{"title":"Exploring the Impact of LRRK2 WD40 G2294R Mutation on Conformation and Dimerisation Dynamics: Insights From Molecular Dynamics Simulation","authors":"Chuancheng Wei, Choon Han Heh, Sek Peng Chin","doi":"10.1002/jcb.70011","DOIUrl":"https://doi.org/10.1002/jcb.70011","url":null,"abstract":"<div>\u0000 \u0000 <p>LRRK2 has gained prominence in treating Parkinson's disease as a potential drug target. Mutations in the WD40 domain, like G2294R, are notable for their influence on the stability and dimerisation of the LRRK2. Studies have shown that G2294R could result in the WD40 distortion and destabilised LRRK2 protein. However, the underlying mechanism remains unclear. To elucidate how the G2294R mutation in the WD40 domain affects the structural and functional conformation of LRRK2, the structure of WD40 G2294R was constructed using homology modelling, and the molecular dynamics simulations on G2294R and wild-type dimers and monomers were carried out. The results show that distortion mainly occurs in the areas of β3, L1, β5, L2, and β7. The dimerisation was enhanced through the conformational changes in the G2294R variant, while the domains show different contributions towards the dimerisation. Our study reveals the effects of G2294R on the WD40. It explores its role in dimerisation and distortion, which could contribute to developing novel WD40 inhibitors and elucidate the molecular mechanism of WD40 dimerisation-monomerisation equilibrium.</p>\u0000 </div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding SARS-CoV-2 Inhibition: Insights From Molecular Dynamics Simulation of Condensed Amino Thiourea Scaffold Small Molecules

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-02-23 DOI: 10.1002/jcb.70005

Xiaoli Shen, Hao Zhang, Pengyin Zhang, Xuerui Zhao, Chang Liu, Jianan Ju, Aijun Liu, Song Wang

{"title":"Decoding SARS-CoV-2 Inhibition: Insights From Molecular Dynamics Simulation of Condensed Amino Thiourea Scaffold Small Molecules","authors":"Xiaoli Shen, Hao Zhang, Pengyin Zhang, Xuerui Zhao, Chang Liu, Jianan Ju, Aijun Liu, Song Wang","doi":"10.1002/jcb.70005","DOIUrl":"https://doi.org/10.1002/jcb.70005","url":null,"abstract":"<div>\u0000 \u0000 <p>The main protease (M<sup>pro</sup>) of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) plays a crucial role in viral replication. In this study, the binding modes and inhibitory mechanisms of eight condensed amino thiourea scaffold inhibitors of M<sup>pro</sup> in proteins were investigated using a combination of molecular docking, molecular dynamics simulations, and MM/PBSA binding free energy calculations. The results indicated that the para-hydroxyl group on the benzene ring at the head of the inhibitor has a decisive influence on the initial docking pose and binding free energy strength of the inhibitor. Additionally, the position and length of the hydrophobic side chain on the tail six-membered ring significantly impacted the final binding pose of the inhibitor. The presence of a long hydrophobic side chain in the ortho position of this ring, through its interaction with the P4 hydrophobic pocket, led to an opposite binding mode in the protein compared with when it was present with or without the para-side chain. Different lengths of para-substituted side chains affected the positioning of the inhibitors in the enzyme. These different binding modes led to variations in the binding free energy between the inhibitor and the protein, which in turn gave rise to differences in inhibitory capability.</p></div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peroxisome Proliferator-Activated Receptor-Gamma Activation by an Active Compound in Lythrum anceps (Koehne) Makino

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-02-21 DOI: 10.1002/jcb.70009

Ryo Miyata, Masanobu Suzuki, Yuka Okazaki, Daigo Abe, Yoshihiro Nakajima

{"title":"Peroxisome Proliferator-Activated Receptor-Gamma Activation by an Active Compound in Lythrum anceps (Koehne) Makino","authors":"Ryo Miyata, Masanobu Suzuki, Yuka Okazaki, Daigo Abe, Yoshihiro Nakajima","doi":"10.1002/jcb.70009","DOIUrl":"https://doi.org/10.1002/jcb.70009","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Lythrum anceps</i> (Koehne) Makino (Japanese common name: “Misohagi”) is an edible plant belonging to the Lythraceae family. It is mainly distributed in Asia, Northern Africa, and Europe. Plants of the genus <i>Lythrum</i> exhibit a broad range of biological activities including anti-inflammatory and antimicrobial activities. Because of this, the plants are used in traditional medicine to treat hemorrhage, infected wounds, and dysentery. The activation of peroxisome proliferator-activated receptor-gamma (PPARγ) is an effective target for improving insulin resistance and anti-inflammatory activity. However, PPARγ activation by the genus <i>Lythrum</i> remains unclear. Aiming to evaluate PPARγ activation by <i>L. anceps</i>, we generated a reporter cell line using an artificial chromosome vector that stably expresses dual-color beetle luciferases. Dual-color real-time bioluminescence monitoring revealed marked PPARγ activation in <i>L. anceps</i> extracts. Moreover, ellagic acid was identified as a PPARγ activator present in <i>L. anceps</i> by a bioassay-guided fractionation approach.</p>\u0000 </div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming Growth Factor-β Modulates Cancer Stem Cell Traits on CD44 Subpopulations in Hepatocellular Carcinoma 转化生长因子-β调节肝细胞癌 CD44 亚群的癌症干细胞特征

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-02-12 DOI: 10.1002/jcb.70003

Mario Alejandro Aguilar-Chaparro, Sonia Andrea Rivera-Pineda, Hury Viridiana Hernández-Galdámez, Emmanuel Ríos-Castro, Olga Lilia Garibay-Cerdenares, Carolina Piña-Vázquez, Saúl Villa-Treviño

{"title":"Transforming Growth Factor-β Modulates Cancer Stem Cell Traits on CD44 Subpopulations in Hepatocellular Carcinoma","authors":"Mario Alejandro Aguilar-Chaparro, Sonia Andrea Rivera-Pineda, Hury Viridiana Hernández-Galdámez, Emmanuel Ríos-Castro, Olga Lilia Garibay-Cerdenares, Carolina Piña-Vázquez, Saúl Villa-Treviño","doi":"10.1002/jcb.70003","DOIUrl":"https://doi.org/10.1002/jcb.70003","url":null,"abstract":"<p>Hepatocellular carcinoma (HCC) is a formidable malignancy, with growing interest in identifying cancer stem cells (CSCs) as potential therapeutic targets. CD44 isoforms have emerged as promising CSC markers in HCC, often associated with epithelial-mesenchymal transition (EMT) induced by transforming growth factor-beta (TGF-β). However, the intricate relationship between CSC traits, CD44 isoforms, and TGF-β effects on CD44 subpopulations in HCC remains unclear. This study aimed to clarify how TGF-β influences proteomic changes and CSC traits in subpopulations expressing standard CD44 isoform (CD44std) and CD44 variant 9 (CD44v9). Treating SNU-423 cells with TGF-β lead to notable morphological changes, resembling a spindle-like phenotype, along with reductions in CD44v9+ subpopulations and differential CD44std expression. Proteomic analysis highlighted significant alterations in signaling pathways, particularly the mitogen-activated protein kinase (MAPK) pathway. Validation experiments demonstrated upregulation in CD44std cells and downregulation in CD44v9 cells post-TGF-β treatment. Furthermore, TGF-β exerted regulatory influence over Sox2 and Nanog expression, resulting in increased colony and spheroid formation in CD44std cells but decreased capabilities in CD44v9 cells. TGF-β also enhanced the migratory and invasive properties of both subpopulations through EMT, alongside increased adhesive abilities in CD44v9 cells. These findings illuminate the dynamic interplay between TGF-β and CD44std/CD44v9 subpopulations, emphasizing the role of MAPK signaling and modulation of CSC traits. This research contributes to understanding the dynamic interplay between CD44 isoforms and TGF-β in HCC.</p>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcb.70003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxic Secretome and Exosomes Derived From Human Glioblastoma Cells (U87MG) Promote Protumorigenic Phenotype of Microglia in Vitro

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-02-04 DOI: 10.1002/jcb.70002

Sangati Pancholi, Ritvi Shah, Utsav Bose, Ankit Yadav, Karthik Murukan, Prakash Pillai

{"title":"Hypoxic Secretome and Exosomes Derived From Human Glioblastoma Cells (U87MG) Promote Protumorigenic Phenotype of Microglia in Vitro","authors":"Sangati Pancholi, Ritvi Shah, Utsav Bose, Ankit Yadav, Karthik Murukan, Prakash Pillai","doi":"10.1002/jcb.70002","DOIUrl":"https://doi.org/10.1002/jcb.70002","url":null,"abstract":"<div>\u0000 \u0000 <p>Glioblastoma multiforme (GBM), a highly heterogeneous CNS tumor known for its highest incidence rates and poor prognosis has shown limited success in the therapies due to hypoxia—driving immune-suppression in the tumor microenvironment (TME). Emerging evidence highlights the involvement of tumor cell-derived exosomes in tumor-associated microglia polarization via transfer of exosomal onco-proteins and miRNAs. Although the regulatory role of long noncoding RNAs (lncRNAs) in immune signaling are known, its mechanism in microglial polarization via exosomes in GBM still remains poorly understood. In our study, we found that in comparison to the normoxic GBM-derived exosomes lncRNA H19 was significantly upregulated in hypoxic GBM-derived exosomes. Hypoxic GBM-derived exosomes and secretome (conditioned media) caused the reduction in the % phagocytosis of microglia as compared with the control group. Moreover, GBM secretome caused increase in the M2-specific genes (IL10, STAT-3, CD163, CD206) in microglia indicating its polarization to the protumorigenic (M2) phenotype. LncRNA H19 knocked down GBM-secretome treatment in microglia further reduced the STAT-3 expression indicating H19 mediated signaling. Overall, our results suggest the involvement of hypoxic exosomes and lncRNA H19 in microglial polarization and H19 as a potential target.</p>\u0000 </div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143111512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteasomal Dysfunction in Cancer: Mechanistic Pathways and Targeted Therapies

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-01-30 DOI: 10.1002/jcb.70000

Pranit Hemant Bagde, Meenakshi Kandpal, Annu Rani, Sachin Kumar, Amit Mishra, Hem Chandra Jha

引用次数: 0

RETRACTION: Kaempferol Increases Apoptosis in Human Acute Promyelocytic Leukemia Cells and Inhibits Multidrug Resistance Genes 转载：堪非醇能增加人类急性早幼粒细胞白血病细胞的凋亡并抑制多药耐药基因。

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-01-27 DOI: 10.1002/jcb.70001

{"title":"RETRACTION: Kaempferol Increases Apoptosis in Human Acute Promyelocytic Leukemia Cells and Inhibits Multidrug Resistance Genes","authors":"","doi":"10.1002/jcb.70001","DOIUrl":"10.1002/jcb.70001","url":null,"abstract":"<p><b>RETRACTION</b>: M. Moradzadeh, A. Tabarraei, H. R. Sadeghnia, A. Ghorbani, A. Mohamadkhani, S. Erfanian, and A. Sahebkar, “Kaempferol Increases Apoptosis in Human Acute Promyelocytic Leukemia Cells and Inhibits Multidrug Resistance Genes,” <i>Journal of Cellular Biochemistry</i> 119, no. 2 (2018): 2288–2297, https://doi.org/10.1002/jcb.26391.</p><p>The above article, published online on October 20, 2017 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Christian Behl; and Wiley Periodicals LLC. The retraction has been agreed upon following an investigation into concerns raised by a third party, which revealed inappropriate image panel duplications between this article (Figure 5A,B) and other articles published by an overlapping group of authors, in which the images represent different experimental conditions. The explanation provided by the authors could not address these concerns. Thus, the editors have lost confidence in the presented data and consider the conclusions of this manuscript substantially compromised. A. Sahebkar disagrees with the retraction, the other co-authors remained unresponsive.</p>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jcb.70001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTION: Long Non-Coding RNA LOC554202 Promotes Laryngeal Squamous Cell Carcinoma Progression Through Regulating MiR-31

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-01-24 DOI: 10.1002/jcb.30695

引用次数: 0

Mcl-1 is a Gatekeeper Molecule to Regulate the Crosstalk Between Ferroptotic Agent-Induced ER Stress and TRAIL-Induced Apoptosis

IF 3 3区生物学

Journal of cellular biochemistry Pub Date : 2025-01-24 DOI: 10.1002/jcb.30681

Young-Sun Lee, Farzaneh Vafaeinik, Lila Mouakkad, Dong-Hyun Kim, Xinxin Song, Lin Zhang, Yong J. Lee

{"title":"Mcl-1 is a Gatekeeper Molecule to Regulate the Crosstalk Between Ferroptotic Agent-Induced ER Stress and TRAIL-Induced Apoptosis","authors":"Young-Sun Lee, Farzaneh Vafaeinik, Lila Mouakkad, Dong-Hyun Kim, Xinxin Song, Lin Zhang, Yong J. Lee","doi":"10.1002/jcb.30681","DOIUrl":"10.1002/jcb.30681","url":null,"abstract":"<div>\u0000 \u0000 <p>We previously reported that ferroptosis interplays with apoptosis through the integration of two independent pathways: the endoplasmic reticulum (ER) stress signaling pathway and the mitochondria-dependent apoptotic signaling pathway. In this study, we investigated a potential gatekeeper molecule, Mcl-1, between the two signal transduction pathways. Morphology studies and cell death analyses confirmed that a combination treatment of ferroptotic agent erastin (ERA) and apoptotic agent TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) synergistically enhances TRAIL-induced apoptosis in human pancreatic adenocarcinoma BxPC3 and human colorectal carcinoma HCT116 cells. We further observed that ERA upregulated the proapoptotic proteins PUMA (p53 upregulated modulator of apoptosis) and NOXA, as well as the anti-apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1). These results suggest that ERA upregulates these molecules which results in maintenance of the balance between them. Interestingly, this balance was offset when BxPC3 cells and HCT116 cells were treated with ERA in combination with TRAIL. Our studies suggest that the imbalance between PUMA and NOXA and Mcl-1 during the combined treatment is responsible for ERA-enhanced TRAIL-induced apoptosis. This hypothesis was tested by employing a HCT116 Mcl-1 knock-in of phosphorylation site mutant (S121A/E125A/S159A/T163A) and investigated the synergistic interaction between the ERA and TRAIL. Along with morphology and cell death studies, immunoblotting analyses revealed that HCT116 Mcl-1 knock-in mutant cells effectively inhibited reduction of Mcl-1 and apoptosis promoted by the combination treatment. Moreover, ERA enhanced Mcl-1 inhibitor-induced apoptosis. Collectively, our studies suggest that Mcl-1 is a gatekeeper molecule between the ER stress pathway and the mitochondria-dependent apoptotic pathway.</p></div>","PeriodicalId":15219,"journal":{"name":"Journal of cellular biochemistry","volume":"126 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0