Journal of Cardiovascular Development and Disease最新文献

{"title":"Predictive Value of Preoperative Morphology Parameters in Patients Undergoing On-Pump and Off-Pump Coronary Artery Bypass Surgery.","authors":"Krzysztof Greberski, Jakub Batko, Paweł Bugajski, Maciej Łuczak, Maciej Brzeziński, Krzysztof Bartuś","doi":"10.3390/jcdd11110375","DOIUrl":"10.3390/jcdd11110375","url":null,"abstract":"<p><strong>Background: </strong>Coronary heart disease is the most common cause of death worldwide. It is responsible for almost a third of deaths in patients over the age of 35. Various biomarkers are currently being studied in detail for their value in predicting postoperative mortality in patients undergoing CABG.</p><p><strong>Aim: </strong>The aim of this study is to analyze the predictive value of certain blood morphological parameters in CABG and off-pump coronary artery bypass grafting (OPCAB).</p><p><strong>Methods: </strong>A total of 520 patients who underwent surgery in two consecutive years and underwent CABG (404) or OPCAB (116) were included in this retrospective study. Gender, age, comorbidities, five-year survival rate, detailed information on hospitalization, surgery, intensive care unit parameters and preoperative blood samples from the cubital vein were recorded. Inverse propensity treatment weighting was applied to adjust for confounding factors at baseline.</p><p><strong>Results: </strong>No differences were found between OPCAB and CABG as an isolated comparison. In the standardized population, patients with abnormal lymphocyte counts had an increased risk of death at one-year and five-year follow-up. In the standardized population, abnormal red blood cell distribution width (RDW-SD), neutrocyte-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) were associated with increased mortality in each period analyzed.</p><p><strong>Conclusions: </strong>Abnormal PLR, RDW-SD and NLR are associated with increased early and late mortality in patients undergoing CABG and OPCAB. Abnormal lymphocytes are only associated with increased late mortality.</p>","PeriodicalId":15197,"journal":{"name":"Journal of Cardiovascular Development and Disease","volume":"11 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11594579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142728898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiac Rehabilitation After Open Heart Surgery: A Narrative Systematic Review.","authors":"Eleni Delimanoli, Olav Muurlink, Pavlos Myrianthefs, Anna Korompeli","doi":"10.3390/jcdd11110376","DOIUrl":"10.3390/jcdd11110376","url":null,"abstract":"<p><strong>Background: </strong>Postoperative cardiac rehabilitation (CR) programs are increasingly recommended by clinicians, but only a minority of patients who have undergone open heart surgery participate in such programs. Participation rates in postoperative CR, if anything, appear to be declining. This systematic review examines the effectiveness of post-operative CR and reveals possible participation barriers.</p><p><strong>Methods: </strong>A search of two scholarly databases for primary research papers published in the last decade examining the impact of post-operative CR was conducted and the resultant papers reviewed.</p><p><strong>Results: </strong>The 21 resulting studies revealed physiological functioning improvement and a reduction in mortality and readmission rates, while highlighting an enhancement in mental status. Some of the studies recognized the need for nutritional support and suggested that age, gender, access to CR centers, and socioeconomic variables impact participation in CR.</p><p><strong>Conclusions: </strong>Post-operative CR participation rates continue to decline despite increasing evidence of the value of the approach.</p>","PeriodicalId":15197,"journal":{"name":"Journal of Cardiovascular Development and Disease","volume":"11 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11594755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142728306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different Proteins as Biomarkers for Sac Shrinkage After Endovascular Aortic Repair of Abdominal Aortic Aneurysms.","authors":"Alexander Zimmermann, Daniela Reitnauer, Yankey Yundung, Anna-Leonie Menges, Lorenz Meuli, Jaroslav Pelisek, Benedikt Reutersberg","doi":"10.3390/jcdd11110374","DOIUrl":"10.3390/jcdd11110374","url":null,"abstract":"<p><strong>Background: </strong>This study aims to identify circulating biomarkers by using proteomic analysis associated with sac shrinkage or expansion in patients undergoing endovascular aneurysm repair (EVAR) for abdominal aortic aneurysms (AAAs).</p><p><strong>Methods: </strong>Plasma samples were analysed from 32 patients treated with EVAR between 10/2009 and 10/2020. Patients were divided into two groups based on postoperative sac behaviour: sac shrinkage (≥5 mm reduction) and no shrinkage (stabilisation or expansion). Proteomic analysis was performed using high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS), with abundant protein depletion to enhance the detection of low-abundant proteins.</p><p><strong>Results: </strong>Of the 32 patients, 20 exhibited sac shrinkage, and 12 showed no shrinkage. Proteomic analysis identified 632 proteins, with significant differential abundance observed after adjusting for relevant clinical parameters. Notably, neurogranin (NRGN) levels were significantly associated with hypertension and smoking, while casein alpha S1 (CSN1S1) levels varied with statin use. Differentially abundant proteins related to aortic diameter included calpastatin, SCUBE3, and ubiquitin-conjugating enzyme E2, among others.</p><p><strong>Conclusions: </strong>Proteomic profiling revealed distinct biomarker patterns associated with sac behaviour in EVAR-treated AAA patients. These findings suggest potential therapeutic targets for enhancing EVAR outcomes and underscore the need for further investigation into the biological mechanisms underlying aneurysm sac shrinkage and stability.</p>","PeriodicalId":15197,"journal":{"name":"Journal of Cardiovascular Development and Disease","volume":"11 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11594911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142728885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex Differences Among Patients Undergoing Transcatheter Tricuspid Valve Repair Using the Edge-to-Edge Technique.","authors":"Mhd Nawar Alachkar, Astrid Eichelsdörfer, Hesham Mady, Andrea Milzi, Rakan Saadoun, Lukas Krygier, Steffen Schnupp, Christian Mahnkopf","doi":"10.3390/jcdd11110372","DOIUrl":"10.3390/jcdd11110372","url":null,"abstract":"<p><strong>Introduction: </strong>Tricuspid valve regurgitation (TR) is more prevalent among females. Transcatheter tricuspid valve repair (TTVR) using the edge-to-edge technique represents an alternative to surgery in patients with severe TR and high surgical risk. This study aims to investigate sex differences among patients undergoing TTVR.</p><p><strong>Methods: </strong>All patients who underwent TTVR at our center were retrospectively included. We compared baseline characteristics, intra-hospital, and one-year outcomes between males and females.</p><p><strong>Results: </strong>A total of 105 consecutive patients underwent TTVR. Females were more prevalent in the study cohort (n = 63, 60%). Coronary artery disease (CAD) was more evident in males than females (71.4% vs. 47.6%, <i>p</i> = 0.016). Left ventricular ejection fraction (LVEF) was also worse in males (48.8 ± 13.4 vs. 58 ± 6.8, <i>p</i> < 0.001). Other clinical characteristics were similar between both groups. The Success of the procedure (88.1% vs. 95.2%, <i>p</i> = 0.177) and intra-hospital mortality (4.8% vs. 11.1%, <i>p</i> = 0.255) were similar among males and females. At one-year follow-up, mortality was similar between both groups (24.3% vs. 25.9%, <i>p</i> = 0.863). Furthermore, hospitalization due to acute heart failure was also similar between both groups (40.5% vs. 37.5%, <i>p</i> = 0.768), as was a composite endpoint of death or hospitalization. In patients with successful procedures and who survived one year, TR severity was comparable between both groups.</p><p><strong>Conclusions: </strong>In our real-world cohort, more females underwent TTVR than males. No difference was observed in outcomes between males and females at one-year follow-up.</p>","PeriodicalId":15197,"journal":{"name":"Journal of Cardiovascular Development and Disease","volume":"11 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11594526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142728915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Females at a Clear Disadvantage with Postoperative Myocardial Infarction Symptoms.","authors":"Sonja Guethoff, Rebekka Kraft, Matthias Riege, Carola Grinninger, Kara Krajewski","doi":"10.3390/jcdd11110371","DOIUrl":"10.3390/jcdd11110371","url":null,"abstract":"<p><strong>Background: </strong>Cardiovascular disease remains the leading cause of death in women. Gender bias and blindness are coming into focus as relevant risk factors for patients. To date, there have been no studies that target surgeons' potential gender bias in recognizing postoperative myocardial infarction (MI).</p><p><strong>Methods: </strong>An online clinical vignette describing a patient with postoperative MI was randomized for gender. Possible diagnoses, the next steps in management, and questions on gender bias were assessed.</p><p><strong>Results: </strong>A total of 205 surveys were analyzed. MI was recognized in 55.6% of the male case studies vs. 32.0% of the female case studies in the first question (<i>p</i> < 0.005). Cardiac diagnostics were initiated significantly more in male case studies (94% vs. 76%, <i>p</i> = 0.001). Female surgeons listed MI as the first diagnosis twice as often as male surgeons overall (43% vs. 23%, <i>p</i> = 0.027). Female surgeons were also more likely to mention MI across the survey at all compared to male surgeons (89% vs. 67%, <i>p</i> = 0.0002). Board-certified surgeons diagnosed MI by the end of the survey significantly more (88.2%) than residents (75.0%) and medical students (75.0%, <i>p</i> = 0.047).</p><p><strong>Conclusions: </strong>Overall, this study was able to demonstrate the presence of both gender bias and gender blindness in surgeons' assessment of postoperative myocardial infarction symptoms with a clear disadvantage for female patients and a superior awareness for female surgeons.</p>","PeriodicalId":15197,"journal":{"name":"Journal of Cardiovascular Development and Disease","volume":"11 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11594784/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142728923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The David Versus the Bentall Procedure for Acute Type A Aortic Dissection.","authors":"Fausto Biancari, Giorgio Mastroiacovo, Mauro Rinaldi, Luisa Ferrante, Timo Mäkikallio, Tatu Juvonen, Giovanni Mariscalco, Zein El-Dean, Matteo Pettinari, Javier Rodriguez Lega, Angel G Pinto, Andrea Perrotti, Francesco Onorati, Konrad Wisniewski, Till Demal, Petr Kacer, Jan Rocek, Dario Di Perna, Igor Vendramin, Daniela Piani, Eduard Quintana, Robert Pruna-Guillen, Joscha Buech, Caroline Radner, Manoj Kuduvalli, Amer Harky, Antonio Fiore, Angelo M Dell'Aquila, Giuseppe Gatti, Lenard Conradi, Mark Field, Arianna Galotta, Daniele Fileccia, Giuseppe Nanci, Sven Peterss","doi":"10.3390/jcdd11110370","DOIUrl":"10.3390/jcdd11110370","url":null,"abstract":"<p><p><b>Background</b>: Type A aortic dissection (TAAD) is a life-threatening condition which requires prompt diagnosis and surgical treatment. When TAAD involves the aortic root, aortic valve-sparing or Bentall procedures are the main surgical treatment options. <b>Method:</b> The subjects of this analysis were 3735 patients included in the European Registry of Type A Aortic Dissection (ERTAAD). Propensity score matching was performed by estimating a propensity score from being treated with the Bentall or the David procedure using multilevel mixed-effects logistics, considering the cluster effect of the participating hospitals. <b>Results:</b> A Bentall procedure was performed in 862 patients, while a David operation was performed in 139 patients. The proportion of aortic root replacement, as well as the different techniques of aortic root replacement, varied significantly between the participating hospitals (<i>p</i> < 0.001). After propensity score matching, we obtained two groups of 115 patients each, and no statistical differences were reported in terms of postoperative outcomes, except for the rate of dialysis, which was higher in the patients requiring a Bentall procedure (17.4% vs. 7.0%, <i>p</i>-value 0.016). In the unmatched cohorts, the David procedure was associated with a lower 10-year mortality rate compared to the Bentall procedure (30.1% vs. 45.6%, <i>p</i>-value 0.004), but no difference was observed after matching (30.0% vs. 43.9%, <i>p</i>-value 0.082). After 10 years, no differences were observed in terms of proximal aortic reoperation (3.9% vs. 4.1%, <i>p</i>-value 0.954), even after propensity score matching (2.8% vs. 1.8%, <i>p</i>-value 0.994). <b>Conclusions:</b> The David and Bentall procedures are durable treatment methods for TAAD. When feasible, it is advisable that the David procedure is performed for acute TAAD by surgeons with experience with this demanding surgical technique.</p>","PeriodicalId":15197,"journal":{"name":"Journal of Cardiovascular Development and Disease","volume":"11 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11594449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142728936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitral Transcatheter Edge-to-Edge Repair in INTERMACS 3-4 Profile Patients with Severe Mitral Regurgitation.","authors":"Simone Frea, Stefano Pidello, Filippo Angelini, Paolo Boretto, Pier Paolo Bocchino, Daniele Melis, Giuseppe Giannino, Elena Cavallone, Francesca Giordana, Sara Rettegno, Carol Gravinese, Giulia De Lio, Guglielmo Gallone, Veronica Dusi, Gianluca Alunni, Antonio Montefusco, Fabrizio D'Ascenzo, Massimo Boffini, Claudia Raineri, Mauro Rinaldi, Gaetano Maria De Ferrari","doi":"10.3390/jcdd11110373","DOIUrl":"10.3390/jcdd11110373","url":null,"abstract":"<p><strong>Background: </strong>Heart transplantation and left ventricular assist device (LVAD) implementation are effective treatments for advanced heart failure (HF), although their use is limited by organ availability and the high incidence of adverse events. The efficacy of mitral transcatheter edge-to-edge repair (TEER) as a bridge to transplantation or as a destination therapy in advanced HF is still debated.</p><p><strong>Methods: </strong>A total of 63 patients with INTERMACS class 3 or 4 with contraindications for LVAD and severe functional mitral regurgitation (FMR) were evaluated for TEER implantation eligibility. The primary endpoint was a composite of death, urgent heart transplantation and LVAD implantation at 12 months.</p><p><strong>Results: </strong>A total of 36 patients underwent TEER, while 27 patients received optimal medical therapy (MT) alone. In the intervention group, 35 patients (97%) were discharged alive. In the MT group, two in-hospital deaths occurred, two patients underwent urgent heart transplantation, and three patients were discharged on inotropes. At the 12-month follow-up, the incidence of the primary endpoint occurring was lower in the TEER group (25% vs. 70%, HR 0.25, 95% CI 0.11-0.60, <i>p</i> < 0.01) and the tolerance to neurohormonal therapy was higher (53% vs. 30%, <i>p</i> = 0.03).</p><p><strong>Conclusions: </strong>In advanced HF patients with INTERMACS profile 3 or 4 and severe FMR, TEER on top of optimal MT was associated with a lower incidence of death, urgent heart transplantation or LVAD implantation at 12 months compared to optimal MT alone.</p>","PeriodicalId":15197,"journal":{"name":"Journal of Cardiovascular Development and Disease","volume":"11 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142728926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of the Percutaneous Coronary Intervention (PCI) and Coronary Artery Bypass Grafting (CABG) Indication and Mortality Rates in Spain from 2010 to 2019.","authors":"Óscar Gasulla, Antonio Sarría-Santamera, Ferran A Mazaira-Font, Cielo García-Montero, Oscar Fraile-Martinez, Diego Cantalapiedra, Manuel F Carrillo-Rodríguez, Belen Gómez-Valcárcel, Miguel Á Ortega, Melchor Álvarez-Mon, Angel Asúnsolo","doi":"10.3390/jcdd11110369","DOIUrl":"10.3390/jcdd11110369","url":null,"abstract":"<p><p>Percutaneous coronary intervention (PCI) and coronary artery bypass grafting (CABG) are the main interventional treatments for coronary artery disease (CAD) patients. Both procedures are constantly being perfected and developed. This study aims to analyze the evolution of intervention mortality rates of PCI and CABG in recent years in Spain. We use a database of all hospital discharges from CABG and PCI procedures in Spain during two periods, between the years 2010 to 2012 and 2016 to 2019. We elaborate two multivariate regression logistic models to test the differences in mortality between the two periods and the two procedures, adjusting the mortality rates by age, gender, and comorbidities. We find strong evidence that CABG significantly reduced mortality rates, especially in complex patients, while PCI remained almost constant. We also discuss how physicians incorporate the improvement in procedures' performance into the decision-making for the recommendation of these two procedures in CAD patient management.</p>","PeriodicalId":15197,"journal":{"name":"Journal of Cardiovascular Development and Disease","volume":"11 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11594947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142728920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the Obesity Paradox: Analysis of New Prognostic Factors in Transcatheter Aortic Valve Implantation Procedure.","authors":"Francesca Ricci, Leonardo Benelli, Monia Pasqualetto, Mario Laudazi, Luca Pugliese, Maria Volpe, Cecilia Cerimele, Carlo Di Donna, Francesco Garaci, Marcello Chiocchi","doi":"10.3390/jcdd11110368","DOIUrl":"10.3390/jcdd11110368","url":null,"abstract":"<p><strong>Scope: </strong>The main purpose of our study was to collect computed tomography (CT) measurements of fat parameters that are significantly related to body mass index (BMI) and evaluate the associations of these measurements and sarcopenia with early and long-term complications after transcatheter aortic valve implantation (TAVI) in order to investigate the existence of the so-called 'obesity paradox' and the role of sarcopenia in this phenomenon.</p><p><strong>Materials and methods: </strong>We analyzed the significance of fat CT measurements in 85 patients undergoing the TAVI procedure and compared these with each other, as well as with quantified CT BMI and fat density measurements. Secondly, we evaluated the associations of BMI, CT measurements of fat, and CT evaluations of skeletal muscle mass with early and long-term complications after 24 months of post-TAVI follow-up.</p><p><strong>Results: </strong>We found positive and significant relationships between fat CT measurements with each other and with BMI and a negative and significant relation between fat density and fat quantity. By comparing the CT measurements of fat and skeletal muscle mass with early and long-term complications after TAVI, we confirmed the existence of the 'obesity paradox' and the poor effect of sarcopenia after the TAVI procedure.</p><p><strong>Conclusions: </strong>We confirm that overweight and obesity are good prognostic factors, and sarcopenia is a poor prognostic factor for outcomes following the TAVI procedure. We focused on the scientific validation of an easy and fast way to measure fat and skeletal muscle mass using CT to better predict the outcomes of patients undergoing TAVI.</p>","PeriodicalId":15197,"journal":{"name":"Journal of Cardiovascular Development and Disease","volume":"11 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11594374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142728911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Insights on the Formation of the Mitral Valve Chordae Tendineae in Fetal Life.","authors":"Meghan Martin, Kate Gillett, Parker Whittick, Sarah Melissa Wells","doi":"10.3390/jcdd11110367","DOIUrl":"10.3390/jcdd11110367","url":null,"abstract":"<p><p>There is an increasing understanding that some mitral valve pathologies have developmental origins. The time course of valvulogenesis varies by animal model; in cattle, the branched chordae tendineae architecture becomes fully developed at full term. The mechanism by which chordae tendineae bifurcate during fetal development remains unknown. The current study presents a detailed description of bovine chordae tendineae formation and bifurcation during fetal development. Analysis of Movat Pentachrome-stained histological sections of the developing mitral valve apparatus was accompanied by micro-CT imaging. TEM imaging of chordae branches and common trunks allowed the measurement of collagen fibril diameter distributions. We observed a proteoglycan-rich \"transition zone\" at the junction between the fetal mitral valve anterior leaflet and chordae tendineae with \"perforations\" lined by MMP1/2 and Ki-67 expressing endothelial cells. This region also contained clusters of proliferating endothelial cells within the bulk of the tissue. We hypothesize this zone marks a region where chordae tendineae bifurcate during fetal development. In particular, perforations created by localized MMP activity serve as a site for the initiation of a \"split\" of a single chordae attachment into two. This is supported by TEM results that suggest a similar population of collagen fibrils runs from the branches into a common trunk. A clear understanding of normal mitral valvulogenesis and its signaling mechanisms will be crucial in developing therapeutics and/or tissue-engineered valve replacements.</p>","PeriodicalId":15197,"journal":{"name":"Journal of Cardiovascular Development and Disease","volume":"11 11","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11594762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142728928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}