Journal of Biosciences最新文献_第3页

O-GlcNAc transferase promotes synaptic assembly independent of catalytic activity in C. elegans. O-GlcNAc转移酶在秀丽隐杆线虫中独立于催化活性促进突触组装。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Mengting Wu, Huihui Jiang, Qian Li, Yunhe Liu, Hongjun Zhang, Zhiyong Shao

引用次数: 0

Analysis of downstream targets of PAX6 and LHX2, fundamental regulators of the developing mammalian neocortex. 哺乳动物新皮层发育的基本调控因子PAX6和LHX2的下游靶点分析。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Mansi Srivastava, Varun Suresh, Shubha Tole

{"title":"Analysis of downstream targets of PAX6 and LHX2, fundamental regulators of the developing mammalian neocortex.","authors":"Mansi Srivastava, Varun Suresh, Shubha Tole","doi":"","DOIUrl":"","url":null,"abstract":"The transcription factors PAX6 and LHX2 play fundamental roles in mammalian cerebral cortical development. Loss of either factor causes depletion of the cortical progenitor pool and reduction of neurogenesis of later-born cortical neurons. We compared the chromatin occupancy of PAX6 and LHX2 and transcriptional dysregulation upon loss of each factor to analyze whether they function via common gene regulatory networks. We identified common direct and indirect targets that were dysregulated either concordantly or discordantly, based on whether their expression showed similar up- or downregulation upon loss of either factor. Finally, we examined single-cell RNA-seq datasets from neocortical progenitors to identify the cell types within which each common direct/indirect and concordantly/discordantly regulated target gene is expressed as cortical progenitors undergo neurogenesis. Our analysis shows that PAX6 and LHX2 have several common targets that suggest similar pathways for progenitor maintenance, but the regulation of neurogenesis may occur via at least partially non-overlapping pathways. Furthermore, each factor functions to suppress the expression of a set of common Cajal-Retzius cell-specific and interneuron-specific genes which are not normally expressed by cortical progenitors. Together, our analysis offers experimentally testable hypotheses for how PAX6 and LHX2 may execute their critical roles.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A jack of all trades: Hermaphrodite-specific serotonergic neuron in C. elegans. 多面手：秀丽隐杆线虫的雌雄同体特异性血清素能神经元。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Patrick Laurent

{"title":"A jack of all trades: Hermaphrodite-specific serotonergic neuron in C. elegans.","authors":"Patrick Laurent","doi":"","DOIUrl":"","url":null,"abstract":"How does a single neuron type shape both short- and long-term behavior? A study reveals how co-transmission by two hermaphrodite-specific neurons (HSNs) of Caenorhabditis elegans produces both transient and long-lasting effects on its egg-laying behavior. A key question in neuroscience is how neurons and circuits integrate information to drive behavior. In the nematode C. elegans, locomotion, feeding, defecation and egg-laying motor programs are all generated by a simple nervous system consisting of 302 neurons whose connectivity patterns are fully described (White et al. 1986). Egg-laying behavior allows dispersal of eggs in the most appropriate locations. The egg-laying circuit consists of two hermaphrodite-specific serotonergic neurons (HSNs) and six ventral cholinergic C-neurons (VCs) connected to the vulval muscle. In addition, uv1 neuroendocrine cells mechanically respond to egg laying by the release of tyramine and neuropeptides. Together, they produce a rhythmic behavior modulated by environmental cues that alternates between egg-laying phases and resting periods (Hardaker et al. 2001; Ringstad and Horvitz 2008; Fenk and de Bono 2015). How can such a simple circuit generate rhythmic and regulated behavior?","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caenorhabditis elegans as a model for studying intercellular communication via extracellular vesicles. 秀丽隐杆线虫作为研究细胞外囊泡细胞间通讯的模型。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Tingting Li, Yunpeng Zhao, Yan Zou, Yue Wang

引用次数: 0

Germline loss diminishes somatic mitochondria but confers preservation of respiratory function during aging and hypothermia. 生殖系丧失减少了体细胞线粒体，但在衰老和低温期间保留了呼吸功能。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Hye Jin Hwang, Francis R G Amrit, Brandon J Berry, Claudette St Croix, Andrew P Wojtovich, Sruti Shiva, Arjumand Ghazi

引用次数: 0

Role of EhLINE1_ORF1p outside retrotransposition: Downregulation causes growth defects in Entamoeba histolytica. EhLINE1_ORF1p在逆转录外的作用：下调导致溶组织内阿米巴生长缺陷。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Devinder Kaur, Shashi Shekhar Singh, Shraddha Sharma, Sudha Bhattacharya

引用次数: 0

Independent control of functional rewiring after axon injury by let-7 miRNA and insulin/insulin-like growth factor signaling pathways. let-7 miRNA和胰岛素/胰岛素样生长因子信号通路对轴突损伤后功能性重布线的独立控制

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Sruthy Ravivarma, Sibaram Behera, Dipanjan Roy, Anindya Ghosh-Roy

{"title":"Independent control of functional rewiring after axon injury by let-7 miRNA and insulin/insulin-like growth factor signaling pathways.","authors":"Sruthy Ravivarma, Sibaram Behera, Dipanjan Roy, Anindya Ghosh-Roy","doi":"","DOIUrl":"","url":null,"abstract":"The capability of neurons to regenerate after injury becomes poor in adulthood. Previous studies indicated that loss of either let-7 miRNA or components of insulin/insulin-like growth factor signaling (IIS) can overcome age-related decline in axon regeneration in Caenorhabditis elegans. In this study, we wanted to understand the relationship between these two pathways in axon regeneration. We found that the simultaneous removal of let-7 and the gene for insulin receptor daf-2 in parallel increased functional recovery involving posterior touch sensation following axotomy of posterior lateral microtubule (PLM) neurons in adulthood. Conversely, the loss of let-7 could bypass the regeneration block due to the loss of DAF-16, a transcriptional mediator of DAF-2. Similarly, the loss of daf-2 could bypass the requirement of LIN-41, a transcriptional co-factor of the let-7 pathway. Our analysis revealed that these two pathways independently control the targeting of the regenerating axon to the ventral nerve cord (VNC), which leads to functional recovery. The computational analysis of the gene expression data revealed that a large number of genes, their interacting modules, and hub genes under the let-7 and IIS pathways are exclusive in nature. Our study highlights a potential to promote neurite regeneration by harnessing the independent gene expression program involving the let-7 and IIS pathways.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of potent inhibitors from traditional rice varieties targeting α-amylase: A computational approach and in vitro validation. 从传统水稻品种中发现α-淀粉酶有效抑制剂：计算方法和体外验证。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Priya Dharshini Veeraragavan, M Karunanithi, Sathya Albert

{"title":"Discovery of potent inhibitors from traditional rice varieties targeting α-amylase: A computational approach and in vitro validation.","authors":"Priya Dharshini Veeraragavan, M Karunanithi, Sathya Albert","doi":"","DOIUrl":"","url":null,"abstract":"Diabetes involves complex metabolic dysregulation and necessitates approaches such as α-amylase inhibition to moderate postprandial glucose spikes and hyperglycemia. Thus, this study scrutinizes the α-amylase inhibitory potential of selected traditional rice varieties (Athur Kichili Samba, Poonkar, Karuppu Kavuni) as well as a modern variety (CO 43) in the cooked condition, using computational approaches alongside in vitro validation. The 156 metabolites obtained from gas chromatography-mass spectrometry analysis, docked with human pancreatic α-amylase (HPA), and based on the binding energies top-five-ranked metabolites were shortlisted (PubChem IDs: 91714169, 13821354, 167795, 2756016, and 3075922). In addition, molecular dynamics simulation was carried out for a timescale of 200 ns to assess protein-ligand complex stability, with parameters such as root mean square deviation, root mean square fluctuation, radius of gyration, solventaccessible surface area, H-bond, and interaction energy. Furthermore, molecular mechanics Poisson- Boltzmann surface area (MM/PBSA) and molecular mechanics generalized Born surface area (MM/GBSA) analyses were performed to validate the selected inhibitors. In vitro studies corroborated inhibitory effectiveness, with Athur Kichili Samba having the highest potency (IC50=4.48 mg/mL), followed by CO 43, Poonkar, and Karuppu Kavuni (IC50=5.12, 6.29, and 11.35 mg/mL). The consistent ranking of antidiabetic functionality observed across both computational and experimental approaches highlights the potential of these traditional rice varieties, suggesting additional clinical investigations to endorse their global consumption.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glutamate metabolism disruption in Johanson-Blizzard syndrome: Insights from C. elegans ubr-1 model. johnson - blizzard综合征中谷氨酸代谢紊乱：来自秀丽隐杆线虫ubr-1模型的见解。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Yi Li, Shangbang Gao

引用次数: 0

Evolution of body size and selfhood: Size-scaling from selfless reproduction to enhanced self-preservation. 体型和自我的进化：从无私繁殖到增强自我保护的体型缩放。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Douglas S Glazier

{"title":"Evolution of body size and selfhood: Size-scaling from selfless reproduction to enhanced self-preservation.","authors":"Douglas S Glazier","doi":"","DOIUrl":"","url":null,"abstract":"Our knowledge of how and why various biological and ecological traits relate (scale) to body size has grown at a rapid pace. In this exploratory analysis, I aim to further advance our knowledge of biological scaling by arguing that individuality and selfhood increase along a size spectrum from tiny microbes to huge trees and whales, as driven by a gradient of decreasing mortality. According to a proposed size-self spectrum (SSS) model, tiny short-lived organisms are continuously engaged in relatively rapid, all-consuming, selfless reproduction, whereas large long-lived organisms have evolved relatively high levels of individuality and selfpreservation. Grades of individuality in organisms along the SSS are recognized by their levels of development of (a) protective boundaries between their inner and outer environments, (b) autonomy and identity preservation, and (c) self-awareness and regulation. Paradoxically, as increasingly large organisms have evolved increased independence from their external environments, they have also exerted greater per-capita impacts on them. With increasing body size, the prevailing direction of influence of an individual organism's internal versus external environments switches from inward in small 'exocentric' species to outward in large 'endocentric' species. Implications of the SSS model for understanding the nature and environmental impact of humans, who are relatively large organisms, as well as various other controversial biological, ecological, and philosophical issues are discussed.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0