Journal of Biosciences最新文献_第3页

Redefining the nitroplast: Recent insights into the endosymbiontto- organelle transition. 重新定义硝基质体：内共生向细胞器转变的最新见解。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Amey J Bhide

{"title":"Redefining the nitroplast: Recent insights into the endosymbiontto- organelle transition.","authors":"Amey J Bhide","doi":"","DOIUrl":"","url":null,"abstract":"One of the most remarkable events in cellular evolution is the endosymbiosis of α-proteobacteria with a single archaean host cell, a rare evolutionary process, which eventually led to the transformation of symbionts into fully functional mitochondrial organelles in eukaryotes. Evolutionary events related to plants occurred almost 1.6 billion years ago, when eukaryotic heterotrophs acquired a β-cyanobacterium (containing 1B RUBISCO) in what is termed as primary endosymbiosis. Further, this composite cell lineage evolved into three photosynthetic lineages: green algae (plants), red algae and the glaucophytes. Thereafter, a secondary, and tertiary endosymbiosis event occurred giving rise to distinct kinds of green and red-derived photosynthetic plastids, which can be observed in a few haptophytes and dinoflagellates respectively. Eventually, these endosymbionts acquired characteristic cellular properties such as two/multiple envelope membranes and reduction of their genomes through either loss or concerted endosymbiotic gene transfer (EGT) into the nucleus, which ultimately led to the decline of more than three quarters of coding capacity and complete loss of several metabolic pathways. This loss, however, is partly compensated by import of nuclearencoded proteins as well as proteins acquired by horizontal gene transfer (HGT). For most proteins, specific transport mechanisms from nucleus/cytoplasm to organelle exist. The proteins are typically translated as a preprotein with specific signal sequences targeted to the organelle membrane. These membranes harbour receptors, in some cases soluble receptors, for recognition of these signal sequences. Proteins are then internalised using a set of translocation machineries (Gould et al. 2006).","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ARP2/3 regulates cell surface dynamics of CeTOCA-1 in C. elegans zygotes. ARP2/3调控秀丽隐杆线虫受精卵中CeTOCA-1的细胞表面动力学。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Aadya Nageswaran, Promiti Mitra, Megha Rai, Diya Anand, Yogesh Pratap, Anup Padmanabhan

{"title":"ARP2/3 regulates cell surface dynamics of CeTOCA-1 in C. elegans zygotes.","authors":"Aadya Nageswaran, Promiti Mitra, Megha Rai, Diya Anand, Yogesh Pratap, Anup Padmanabhan","doi":"","DOIUrl":"","url":null,"abstract":"Polymerization of branched actin networks by the ARP2/3 complex plays a critical role in diverse cellular processes. ARP2/3 activity is tightly controlled by the upstream CDC-42 GTPase and effectors such as the Wiscott-Aldrich syndrome protein (N-WASP/Wiscott-Aldrich Syndrome Protein (WSP-1)) and members of the F-BAR containing transducer of CDC-42-dependent actin assembly (TOCA) protein family. While the mechanisms governing WASP/N-WASP (neural-WASP) functioning are well understood, the regulatory dynamics of TOCA proteins at the cell cortex remain poorly characterized. Here, using the Caenorhabditis elegans zygote as a model system, we investigated the role of cortical F-actin structures - both branched and linear - in modulating surface dynamics of CeTOCA-1, the nematode ortholog of mammalian TOCA-1. In our in silico analysis, iPTM values associated with the interaction between different domains of CeTOCA-1 and CDC-42 suggested that while the HR-1 domain is essential for this interaction, the SH3 domain is dispensable for complex formation between the two proteins. Further, we experimentally disrupted ARP2/3 and CYK-1/ formin-polymerized F-actin structures in C. elegans zygotes to examine the role of cortical F-actin on CeTOCA-1 assembly dynamics and biophysical properties. Co-localization studies revealed a preferential association between CeTOCA-1 and the pool of F-actin structures polymerized by ARP2/3. Disruption of ARP2/3 led to the formation of larger CeTOCA-1 clusters, prolonged cluster lifetime on the cell surface, and reduced cluster mobility. These findings suggest that distinct F-actin structures play specialized roles in mediating plasma membrane interactions and regulating surface dynamics of CeTOCA-1 clusters.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of exosomes derived from induced and human adipose tissue-derived mesenchymal stem cells on human cancer cells. 诱导和人脂肪组织来源的间充质干细胞外泌体对人癌细胞的影响。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Razan Aldiqs, Sura Nashwan, Mohammad A Ismail, Tareq Saleh, Raghda Barham, Malik Zihlif, Nidaa A Ababneh

{"title":"Effect of exosomes derived from induced and human adipose tissue-derived mesenchymal stem cells on human cancer cells.","authors":"Razan Aldiqs, Sura Nashwan, Mohammad A Ismail, Tareq Saleh, Raghda Barham, Malik Zihlif, Nidaa A Ababneh","doi":"","DOIUrl":"","url":null,"abstract":"Exosomes (Exos) derived from mesenchymal stem cells (MSCs) are known to influence cancer cell behavior; however, the clinical use of MSCs is limited due to the gradual loss of their differentiation potential with continuous passaging. Induced mesenchymal stem cells (iMSCs) have emerged as a promising alternative source, but the effects of Exos derived from iMSCs (iMSC-Exos) on cancer cells remain incompletely understood. This study aims to compare the effects of iMSC-Exos with ADMSC-Exos derived from adipose tissue-derived mesenchymal stem cells (ADMSCs) on the viability, invasion, and migration of breast (MCF7) and lung (A549) cancer cells. Conditioned media from iMSCs and ADMSCs were collected for isolation and characterization of Exos. MCF7 and A549 cell lines were treated with iMSC- and ADMSC-Exos, and Exos uptake, cell viability, migration, senescence, and expression of BAX and BCL-2 genes were evaluated. iMSCand ADMSC-Exos were successfully internalized into cancer cells, with a higher efficiency of ADMSC-Exos uptake in MCF7 cells. Cell viability decreased and migration increased in both cancer cell lines upon treatment. BAX expression was significantly reduced in MCF7 cells following ADMSC-Exos treatment and in A549 cells after iMSC-Exos treatment. In contrast, BCL-2 expression was significantly reduced in MCF7 cells treated with both iMSC- and ADMSC-Exos, while it significantly increased in A549 cells after ADMSC-Exos treatment. A549 lung cancer cells showed a higher level of senescence than MCF7 breast cancer cells, particularly when treated with iMSC-Exos. Minimal overall differences were observed in viability, apoptosis, and migration assays between iMSC- and ADMSC-Exos in MCF7 and A549 cells. However, significant differences were observed in the senescence and expression of BAX and BCL-2 genes across cancer cell lines. These findings highlight the importance of further investigation into the distinct effects of iMSC- and ADMSC-Exos on cancer cell biology.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors shaping resource partitioning in predators of clumped patches of bamboo woolly aphids. 竹绵蚜成片地捕食者资源分配的影响因素。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Basant Kumar Agarwala

{"title":"Factors shaping resource partitioning in predators of clumped patches of bamboo woolly aphids.","authors":"Basant Kumar Agarwala","doi":"","DOIUrl":"","url":null,"abstract":"Prey-predator interactions favor the aggregation of generalist predators adept at avoiding competition in foraging on temporary food resources. There is scant information on the predator guild on clumpy patches of woolly aphids on bamboo host plants in forested landscapes. Results of a field-cum-laboratory study explain the aggregation of generalist and specialist predators in patchy resources of the bamboo-feeding woolly aphid, Ceratovacuna silvestrii, with particular reference to the specialist giant ladybird predator, Anisolemnia dilatata. This predator's larvae share food resources for 26 weeks including 11 weeks of coexistence with 2 small-sized generalist predators and 11 weeks with the larvae of specialist moth predator. Results show a preference for low prey density patches by small-sized predators in contrast to high prey density by the giant ladybird predator. Between the two woolly aphid prey-specialist predators, the moth caterpillars (Dipha aphidivora) avoided competition with the giant ladybirds by foraging in silken nests in less aggregated aphid patches. Eggs and larvae of the giant ladybird predator are defended from heterospecific and conspecific predators. This trait deters other predators of the guild that avoid prey patches visited by the giant ladybirds. Bigger size, preference for high-density prey patches, and anti-predation trait of eggs and larvae confer selection advantages to giant ladybirds as the top predator of the guild of woolly aphids. This has evolutionary significance for the ecological stability of prey-predator dynamics in a forested landscape.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isa2p is essential in Saccharomyces cerevisiae for mitochondrial function and stress resistance by allowing Rip1p subunit assembly into cytochrome bc₁ complex. 通过允许Rip1p亚基组装到细胞色素bc1复合体中，Isa2p在酿酒酵母菌的线粒体功能和抗逆性中是必不可少的。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Luis A Sánchez-Briones, Mauricio Gomez-Gallardo, Christian Cortes-Rojo, Elizabeth Sánchez-Duarte, Jesús Campos-García

{"title":"Isa2p is essential in Saccharomyces cerevisiae for mitochondrial function and stress resistance by allowing Rip1p subunit assembly into cytochrome bc1 complex.","authors":"Luis A Sánchez-Briones, Mauricio Gomez-Gallardo, Christian Cortes-Rojo, Elizabeth Sánchez-Duarte, Jesús Campos-García","doi":"","DOIUrl":"","url":null,"abstract":"In Saccharomyces cerevisiae, the iron-sulfur cluster biogenesis late-acting subsystem (Fe-S-IBG) comprises the mitochondrial glutaredoxin (Grx5), Isa1, Isa2, and iron-sulfur cluster assembly factor IBA57 (Iba57) proteins. The Fe-S-IBG subsystem assists in inserting [4Fe-4S] clusters into apoproteins, some of which belong to the electron transport chain (ETC). However, whether the Fe-S-IBG subsystem indirectly stabilizes respiratory supercomplexes and proper ETC function via insertion of [Fe-S] proteins into ETC complexes remains to be elucidated. We compared the effects of ISA2- and GRX5-independent mutations on the insertion of Rip1p, a [2Fe-2S]-containing protein involved in both electron transfer in the bc1 complex and the formation of respiratory supercomplexes. The levels of Rip1p, supercomplex assembly, ETC function, oxidative stress, and resistance of yeast to ethanol stress were evaluated on haploid S. cerevisiae cells with independent mutations of the ISA2 and GRX5 genes. Susceptibility to ethanol was increased in the isa2Δ and grx5Δ mutants, which was associated with enhanced glutathione oxidation due to higher levels of free iron and increased oxidants. Furthermore, the isa2Δ mutant showed decreased Rip1p expression, respiratory dysfunction, and defective respiratory supercomplex formation, which was restored by ISA2 complementation. These results suggest that Isa2p is essential for proper respiratory chain function and resistance to oxidative stress by stabilizing supercomplexes in a manner dependent on Rip1p insertion in the cytochrome bc1 complex.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assay medium composition affects Caenorhabditis elegans thermotaxis behaviours. 实验培养基组成影响秀丽隐杆线虫的趋热性行为。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Yanshu Zhang, Masami Shima, Yuki Aoki, Asuka Takeishi

{"title":"Assay medium composition affects Caenorhabditis elegans thermotaxis behaviours.","authors":"Yanshu Zhang, Masami Shima, Yuki Aoki, Asuka Takeishi","doi":"","DOIUrl":"","url":null,"abstract":"Animals exhibit behavioural responses to environmental stimuli. To date, various experimental assays have been conducted to investigate the molecular and neural mechanisms underlying behavioural responses in Caenorhabditis elegans. However, behavioural outcomes can vary even under the same stimulus due to factors such as past experience, individual circumstances, and modifications in behavioural assay procedures, which complicate the interpretation of results. In this study, we focussed on the composition of the behavioural assay media and compared the behaviours of C. elegans in response to temperature and odour stimuli using three commonly used media: nematode growth medium (NGM), thermotaxis (TTX), and chemotaxis (Chemo) assay media. Our results demonstrated that in thermotaxis, C. elegans exhibited stable behavioural decisions across all examined media. However, the population of worms reaching the preferable areas was smaller in Chemo and TTX media compared with NGM due to prolonged exploration period and reduced migration speed. By contrast, in chemotaxis, we did not observe significant differences across the tested media. This study provides insights into the significant effects on C. elegans behaviour caused by the modifications of the behavioural assay media and emphasises the importance of further studies to explore the detailed neural mechanisms that regulate animal behaviour under diverse environmental factors.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Why does community ecology need chemistry? Because keystone molecules can govern food webs. 为什么群落生态学需要化学？因为关键分子可以控制食物网。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Renee M Borges

{"title":"Why does community ecology need chemistry? Because keystone molecules can govern food webs.","authors":"Renee M Borges","doi":"","DOIUrl":"","url":null,"abstract":"In an earlier editorial (Borges 2022), I had talked about the keystone concept formulated by Robert Paine. This important concept (Paine 1966) emphasised the idea that biodiversity is maintained by a top-down process in which the removal of a keystone predator would allow the unchecked proliferation of herbivores; during this process a dominant herbivore might outcompete another herbivore species, resulting in the removal of the lesser competitor from the community and a reduction in overall species richness (i.e., the number of species present). The keystone predator concept is well-established in community ecology. Are there keystone molecules whose presence can influence the diversity of communities? At an extreme, of course, one may say that oxygen is a most important molecule whose presence has fuelled the diversification of autotrophs such as diatoms and plants, and hence the flowering of life. A key feature of Paine's keystone concept is that the species must exert an impact that is disproportionate to its abundance (Power et al. 1996). By this definition, oxygen, although vital to all life, would fail to be recognised as a keystone chemical or molecule in species communities. Are there chemical compounds that can govern species diversity within trophic levels of an ecological community, as defined by Paine's keystone concept?","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of public DNA-Seq data in mitogenomics research: A case study on the flying fish family (Beloniformes: Exocoetidae). 公开DNA-Seq数据在有丝分裂基因组学研究中的作用：以飞鱼科（飞鱼目：外鱼科）为例。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Iuri Batista DA Silva, Igor Henrique Rodrigues-Oliveira, Karine Frehner Kavalco, Fabiano Bezerra Menegidio, Rubens Pasa

{"title":"Role of public DNA-Seq data in mitogenomics research: A case study on the flying fish family (Beloniformes: Exocoetidae).","authors":"Iuri Batista DA Silva, Igor Henrique Rodrigues-Oliveira, Karine Frehner Kavalco, Fabiano Bezerra Menegidio, Rubens Pasa","doi":"","DOIUrl":"","url":null,"abstract":"Flying fishes (Exocoetidae) are a highly specialized group of Beloniformes, with 4 subfamilies, 7 genera, and 78 species. However,only a small number of species of flying fishes have the mitochondrial genome described sof ar. Considering the importance of mitogenomes in evolution, in this study we aimed to expand the description of mitochondrial genomes and the phylogenetic relationships of Exocoetidae. We used publicly available DNA-Seq libraries to assemble the mitochondrial genomes of 7 species of flying fishes. The mitochondrial genomes of Exocoetidae showed conserved features among all species. With 22 tRNAs, 2 rRNAs, and 13 protein-coding genes, they share common features among vertebrates.Cheilopogon was reconstructedaspolyphyletic, with four clades, which reinforces previous studies that recovered Cheilopogon as non-monophyletic.Werecoveredallfour subfamilies as monophyletic,with Parexocoetinae as the sister group of all other subfamilies.The genetic distance between Exocoetidae species was two times smaller than the genetic distance among the Hyporhamphus species, indicating a low genetic divergence in the family. Hereby, this study expanded the knowledge of mitochondrial genome features and presented one of the most comprehensive mitochondrial genome datasets used for studying flying fishestodate. This was achievable using publicly available data, reinforcing the importanceof the re-use of such data in the fields of mitogenomics and phylogenetics.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced whole-brain calcium imaging and cell identification in C. elegans reveal AWC^OFF neuronal responses to 2-nonanone. 秀丽隐杆线虫全脑钙成像和细胞鉴定显示AWCOFF神经元对2-壬烷酮的反应。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Yuto Endo, Ryoga Suzuki, Taira Ito, Reina E Itoh, Ryota Kawaguchi, Koutarou D Kimura

{"title":"Enhanced whole-brain calcium imaging and cell identification in C. elegans reveal AWCOFF neuronal responses to 2-nonanone.","authors":"Yuto Endo, Ryoga Suzuki, Taira Ito, Reina E Itoh, Ryota Kawaguchi, Koutarou D Kimura","doi":"","DOIUrl":"","url":null,"abstract":"Whole-brain calcium imaging combined with multicolor cell identification is a revolutionary technique for elucidating structure-function relationships in the neural network of the nematode Caenorhabditis elegans. Existing genetically encoded calcium indicator (GCaMP) (for calcium imaging) + neuronal polychromatic atlas of landmarks for whole-brain imaging (NeuroPAL) (for cell identification) strains, however, exhibit suboptimal behavioral and neural responses to external stimuli, at least under certain conditions. To address this, we established a new strain of GCaMP + NeuroPAL, KDK94, and found that this new strain showed improved behavioral and/or neural responses to the repulsive odor 2-nonanone and electric stimuli compared with existing strains. Whole-brain calcium and NeuroPAL imaging using the new strain with several technical improvements revealed that in addition to the previously known amphid sensory neuron (ASH) and amphid wing 'B' cells (AWB) sensory neurons, the AWCOFF neuron responds to both stepwise increases in the water phase and subtle gradual increases in the air phase of 2-nonanone concentration. The improvement of the whole-brain imaging system with cell identification and the transgenic strain for the system may provide new insights into the neural circuit dynamics underlying the basic brain functions, such as learning, decisionmaking, and emotion, of C. elegans.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of a novel marine Aspergillus fumigatus strain AFK11 and inhibition of inflammatory mediators. 新型海洋烟曲霉AFK11菌株的分离及对炎症介质的抑制作用。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Kranti Kiran Reddy Ealla, Neema Kumari, Se-Kwon Kim, Nimisha Rawat, Anuradha Mokkapati, Ira Bhatnagar

{"title":"Isolation of a novel marine Aspergillus fumigatus strain AFK11 and inhibition of inflammatory mediators.","authors":"Kranti Kiran Reddy Ealla, Neema Kumari, Se-Kwon Kim, Nimisha Rawat, Anuradha Mokkapati, Ira Bhatnagar","doi":"","DOIUrl":"","url":null,"abstract":"Marine fungi are the leading producers of bioactive metabolites with medicinal properties against several diseases. However, since marine species survive in diverse climates, they are usually difficult to culture in laboratory setups, making them challenging to study. This study aimed to optimize culture conditions for marine fungi and evaluate the inhibitory action of the marine extract on primary pro-inflammatory cytokines. Culture conditions for marine fungi were optimized using biomass production, hyphal density, and radial extension, considering temperature and humidity. The study isolated a new Aspergillus fumigatus variant, AFK11 (GenBank accession no. JX022941), showing 96% similarity to reported strains. Ethyl acetate extracts of AFK11 significantly inhibited primary pro-inflammatory cytokines, tumor necrosis factor-α, and interleukin-6, in a dose-dependent manner. This research highlights the successful laboratory culture and anti-inflammatory potential of the new strain of Aspergillus, AFK11, emphasizing the need for further research for its potential in various other diseases such as cancer.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0