Journal of Biosciences最新文献_第3页

Cell membrane proteome analysis in HEK293T cells challenged with α-synuclein amyloids 用α-突触核蛋白淀粉样蛋白挑战 HEK293T 细胞的细胞膜蛋白质组分析

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-07-05 DOI: 10.1007/s12038-024-00457-4

Harshit Vaish, Shemin Mansuri, Aanchal Jain, Swasti Raychaudhuri

{"title":"Cell membrane proteome analysis in HEK293T cells challenged with α-synuclein amyloids","authors":"Harshit Vaish, Shemin Mansuri, Aanchal Jain, Swasti Raychaudhuri","doi":"10.1007/s12038-024-00457-4","DOIUrl":"https://doi.org/10.1007/s12038-024-00457-4","url":null,"abstract":"Amyloids interact with plasma membranes. Extracellular amyloids cross the plasma membrane barrier. Internalized extracellular amyloids are reported to trigger amyloidogenesis of endogenous proteins in recipient cells. To what extent these extracellular and intracellular amyloids perturb the plasma membrane proteome is not investigated. Using α-synuclein as a model amyloid protein, we performed membrane shaving followed by mass spectrometry experiments to identify the conformational changes in cell surface proteins after extracellular amyloid challenge. We also performed membrane proteomics after the biogenesis of intracellular α-synuclein amyloids. Our results suggest that promiscuous interactions with extracellular amyloids stochastically alter the conformation of plasma membrane proteins. This affects the biological processes through the plasma membrane and results in loss of cell viability. Cells that survive the extracellular amyloid shock can grow normally and gradually develop intracellular amyloids which do not directly impact the plasma membrane proteome and associated biological processes. Thus, our results suggest that α-synuclein amyloids can damage the plasma membrane and related processes during cell-to-cell transfer and not during their intracellular biogenesis.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"37 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141547708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neural pathways that compel us to scratch an itch 迫使我们抓痒的神经通路

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-07-05 DOI: 10.1007/s12038-024-00452-9

Jagat Narayan Prajapati, Prannay Reddy, Arnab Barik

引用次数: 0

Comparative assessment of two in-house-built isothermal assays for visual detection of African swine fever virus 用于目测检测非洲猪瘟病毒的两种内部自制等温测定法的比较评估

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-06-28 DOI: 10.1007/s12038-024-00451-w

Gyanendra Singh Sengar, Soumendu Chakravarti, Rajib Deb, Seema Rani Pegu, Pranav Anjaria, Joyshikh Sonowal, Swaraj Rajkhowa, Pranab Joyti Das, Vivek Kumar Gupta

{"title":"Comparative assessment of two in-house-built isothermal assays for visual detection of African swine fever virus","authors":"Gyanendra Singh Sengar, Soumendu Chakravarti, Rajib Deb, Seema Rani Pegu, Pranav Anjaria, Joyshikh Sonowal, Swaraj Rajkhowa, Pranab Joyti Das, Vivek Kumar Gupta","doi":"10.1007/s12038-024-00451-w","DOIUrl":"https://doi.org/10.1007/s12038-024-00451-w","url":null,"abstract":"Owing to the lack of effective vaccines, current control measures and eradication strategies for the African swine fever virus (ASFV) rely on early detection and stringent stamping-out procedures. In the present study, we developed two independent isothermal amplification assays, namely, loop-mediated isothermal amplification (LAMP) and polymerase spiral reaction (PSR), for quick visualization of the ASFV genome in clinical samples. Additionally, a quantitative real-time PCR (qRT-PCR)-based hydrolysis probe assay was developed for comparative assessment of sensitivity with the developed isothermal assays. The analytical sensitivity of the LAMP, PSR, and qRT-PCR was found to be 2.64 ×105 copies/µL, 2.64 ×102 copies/µL, and 2.64 ×101 copies/µL, respectively. A total of 165 clinical samples was tested using the developed visual assays. The relative accuracy, relative specificity, and relative diagnostic sensitivity for LAMP vs PSR were found to be 95.37% vs 102.48%, 97.46% vs 101.36%, and 73.33% vs 113.33%, respectively.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"147 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the propensity of various genetic disorders and syndromes in the Koraga, an aboriginal tribe from southern India 揭示印度南部土著部落 Koraga 中各种遗传疾病和综合征的倾向

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-06-22 DOI: 10.1007/s12038-024-00458-3

Swathy M Krishna, Suneetha M Prabhu, Ranajit Das

{"title":"Unraveling the propensity of various genetic disorders and syndromes in the Koraga, an aboriginal tribe from southern India","authors":"Swathy M Krishna, Suneetha M Prabhu, Ranajit Das","doi":"10.1007/s12038-024-00458-3","DOIUrl":"https://doi.org/10.1007/s12038-024-00458-3","url":null,"abstract":"Koragas, recognized as a particularly vulnerable tribal group (PVTG) by the Government of India, are from coastal Karnataka and Kerala. They are experiencing severe socioeconomic and health-related issues and rapid depopulation. The unique genetic makeup of Koragas has been maintained by the practice of endogamy. We aimed to identify genetic factors potentially associated with the predisposition of Koragas towards genetic and multifactorial disorders. We employed genome-wise data of 29 Koraga individuals genotyped on the Infinium Global Screening Array-24 v3.0 BeadChip platform and performed various population genetic analyses including kinship, identity by descent (IBD), and runs of homozygosity (RoH). A high degree of haplotype sharing among the Koraga participants may be indicative of a recent founder event. We identified genetic variants and genes associated with several genetic disorders, higher infant mortality rate, neurological disorders, deafness, and lower fertility rate of this agrarian tribe. Ours is the first genome-wide study on the Koraga tribe that identified genetic factors associated with various genetic disorders. Our findings can provide public healthcare providers with essential genetic information that can be useful in augmenting medical and healthcare services and improving the quality of life of Koragas.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"6 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141530158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collaborative action between noradrenergic and serotoninergic systems in peripheral antinociception in mice 去甲肾上腺素能系统和血清素能系统在小鼠外周抗痛觉中的协同作用

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-06-19 DOI: 10.1007/s12038-024-00461-8

Danielle Diniz Aguiar, Cristina da Costa Oliveira, Júlia Alvarenga Petrocchi, Marina Gomes Miranda E Castor, Andrea Castro Perez, Igor Dimitri Gama Duarte, Thiago Roberto Lima Romero

{"title":"Collaborative action between noradrenergic and serotoninergic systems in peripheral antinociception in mice","authors":"Danielle Diniz Aguiar, Cristina da Costa Oliveira, Júlia Alvarenga Petrocchi, Marina Gomes Miranda E Castor, Andrea Castro Perez, Igor Dimitri Gama Duarte, Thiago Roberto Lima Romero","doi":"10.1007/s12038-024-00461-8","DOIUrl":"https://doi.org/10.1007/s12038-024-00461-8","url":null,"abstract":"Noradrenaline (NA) and serotonin (5-HT) induce nociception and antinociception. This antagonistic effect can be explained by the dose and type of activated receptors. We investigated the existence of synergism between the noradrenergic and serotonergic systems during peripheral antinociception. The paw pressure test was performed in mice that had increased sensitivity by intraplantar injection of prostaglandin E2 (PGE2). Noradrenaline (80 ng) administered intraplantarly induced an antinociceptive effect, that was reversed by the administration of selective antagonists of serotoninergic receptors 5-HT1B isamoltan, 5-HT1D BRL15572, 5-HT2A ketanserin, 5-HT3 ondansetron, but not by selective receptor antagonist 5-HT7 SB-269970. The administration of escitalopram, a serotonin reuptake inhibitor, potentiated the antinociceptive effect at a submaximal dose of NA. These results, indicate the existence of synergism between the noradrenergic and serotonergic systems in peripheral antinociception in mice.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"44 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative analysis of fibrillar collagen organization in the immediate proximity of embedded fibroblasts in 3D collagen hydrogels 对三维胶原水凝胶中嵌入成纤维细胞附近的纤维胶原组织进行定量分析

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-06-17 DOI: 10.1007/s12038-024-00449-4

Shaunak Kanade, Milie Desai, Neel Bhatavadekar, Nagaraj Balasubramanian

{"title":"Quantitative analysis of fibrillar collagen organization in the immediate proximity of embedded fibroblasts in 3D collagen hydrogels","authors":"Shaunak Kanade, Milie Desai, Neel Bhatavadekar, Nagaraj Balasubramanian","doi":"10.1007/s12038-024-00449-4","DOIUrl":"https://doi.org/10.1007/s12038-024-00449-4","url":null,"abstract":"Fibroblasts embedded in a 3D matrix microenvironment can remodel the matrix to regulate cell adhesion and function. Collagen hydrogels are a useful in vitro system to study cell–matrix interactions in a 3D microenvironment. While major matrix reorganizations are easily recognizable, subtle changes in response to environmental or biochemical cues are challenging to discern in 3D hydrogels. Three-dimensional collagen gels at 1.0 mg/ml vs 1.5 mg/ml were labelled with DQ-collagen and imaged by confocal reflectance microscopy to evaluate these small changes. An image analysis pipeline was developed, hydrogel area and number of cross-sections analysed were optimized, and fibrillar collagen properties (number of branches, number of junctions, and average branch length) were quantified. While no significant changes were seen in fibrillar collagen organization between 1.0 mg/ml and 1.5 mg/ml collagen hydrogels, embedded mouse fibroblasts caused a significant increase in collagen branching and organization. Using the phalloidin-labelled cells, this change was quantitated in immediate proximity of the cell. A distinct increase in branch and junction numbers was observed, significantly altered by small changes in collagen concentration (1.0 mg/ml vs 1.5 mg/ml). Together, this analysis gives a quantitative evaluation of how cells respond to and modify their immediate microenvironment in a 3D collagen hydrogel.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"20 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the innate immune system by revolutionizing macrophage-mediated cancer immunotherapy 通过革新巨噬细胞介导的癌症免疫疗法来利用先天性免疫系统

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-06-04 DOI: 10.1007/s12038-024-00441-y

Gayatri Reghu, Praveen Kumar Vemula, Sarita Ganapathy Bhat, Sreeja Narayanan

{"title":"Harnessing the innate immune system by revolutionizing macrophage-mediated cancer immunotherapy","authors":"Gayatri Reghu, Praveen Kumar Vemula, Sarita Ganapathy Bhat, Sreeja Narayanan","doi":"10.1007/s12038-024-00441-y","DOIUrl":"https://doi.org/10.1007/s12038-024-00441-y","url":null,"abstract":"Immunotherapy is a promising and safer alternative to conventional cancer therapies. It involves adaptive T-cell therapy, cancer vaccines, monoclonal antibodies, immune checkpoint blockade (ICB), and chimeric antigen receptor (CAR) based therapies. However, most of these modalities encounter restrictions in solid tumours owing to a dense, highly hypoxic and immune-suppressive microenvironment as well as the heterogeneity of tumour antigens. The elevated intra-tumoural pressure and mutational rates within fast-growing solid tumours present challenges in efficient drug targeting and delivery. The tumour microenvironment is a dynamic niche infiltrated by a variety of immune cells, most of which are macrophages. Since they form a part of the innate immune system, targeting macrophages has become a plausible immunotherapeutic approach. In this review, we discuss several versatile approaches (both at pre-clinical and clinical stages) such as the direct killing of tumour-associated macrophages, reprogramming pro-tumour macrophages to anti-tumour phenotypes, inhibition of macrophage recruitment into the tumour microenvironment, novel CAR macrophages, and genetically engineered macrophages that have been devised thus far. These strategies comprise a strong and adaptable macrophage-toolkit in the ongoing fight against cancer and by understanding their significance, we may unlock the full potential of these immune cells in cancer therapy.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"25 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141257914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first embryo, the origin of cancer and animal phylogeny. III. The totipotency as revealed by morphogenesis and the neoplasia controlled by cellular differentiation 第一个胚胎、癌症的起源和动物系统发育。III.形态发生揭示的全能性和细胞分化控制的肿瘤性

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-06-04 DOI: 10.1007/s12038-024-00445-8

Jaime Cofre

{"title":"The first embryo, the origin of cancer and animal phylogeny. III. The totipotency as revealed by morphogenesis and the neoplasia controlled by cellular differentiation","authors":"Jaime Cofre","doi":"10.1007/s12038-024-00445-8","DOIUrl":"https://doi.org/10.1007/s12038-024-00445-8","url":null,"abstract":"We have extensively described that the neoplastic process (NP) has deep evolutionary roots and we have made specific predictions about the connection between cancer and the formation of the first embryo, which allowed for the evolutionary radiation of metazoans. My main hypothesis is that the NP is at the heart of cellular mechanisms responsible for animal morphogenesis, and given its embryological basis, also at the center of cell differentiation—one of the most interesting and relevant aspects of embryogenesis. In this article, I take forward the idea of the role of physics in the modeling of the neoplastic functional module (NFM) and its contribution to morphogenesis to reveal the totipotency of the zygote. In my consideration of these arguments, I examine mechanical and biophysical clues and their intimate connection with cellular differentiation. I expound on how cancer biology is perfectly intertwined with embryonic differentiation and why it is considered a disease of cell differentiation. The neoplasia is controlled by textural gradients that lead to cell differentiation within the embryo. Thus, the embryo would be a benign tumor. Finally, inspired by evolutionary history and by what the nervous system represents for current biology and based on the impressive nervous system of ctenophores as seen in fossil records, I propose a hypothesis with physical foundations (mechanical morphogenesis) for the formation of a preneural pattern of the nervous system of the first animal embryo.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"43 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141259795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Milk supply chain as a reservoir of antimicrobial-resistant Staphylococcus species 牛奶供应链是耐抗菌葡萄球菌的储存库

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-05-25 DOI: 10.1007/s12038-024-00422-1

K Supriya, Nagappa Karabasanavar, C B Madhavaprasad, G K Sivaraman, P S Girish, Prashantkumar Waghe, A M Kotresh, Mohamed Nadeem Fairoze

{"title":"Milk supply chain as a reservoir of antimicrobial-resistant Staphylococcus species","authors":"K Supriya, Nagappa Karabasanavar, C B Madhavaprasad, G K Sivaraman, P S Girish, Prashantkumar Waghe, A M Kotresh, Mohamed Nadeem Fairoze","doi":"10.1007/s12038-024-00422-1","DOIUrl":"https://doi.org/10.1007/s12038-024-00422-1","url":null,"abstract":"Milk is a source of essential nutrients, but food safety across the milk supply chain has emerged as an integral part of food trade. Of the several food safety hazards, antimicrobial-resistant Staphylococcus species have emerged as one of the major microbial hazards with significant public health concerns. The present cross-sectional study was undertaken with the objective to isolate Staphylococcus species from the milk supply chain, characterize isolates for antimicrobial resistance, and trace the origin of isolates using molecular techniques. Samples collected from the formal and informal milk supply chains showed prevalence of Staphylococcus species of 4.3% (n=720); isolates were identified as coagulase-positive (S. aureus 67.7% and S. intermedius 6.4%) and coagulase-negative (S. lentus 9.6%, S. sciuri 3.2%, S. xylosus 3.2%, S. schleiferi 3.2%, S. felis 3.2%, and S. gallinarum 3.2%) species. Staphylococcus isolates showed antimicrobial resistance to methicillin (32.2%), β-lactam (41.9%), and macrolide-lincosamide-streptogramin B (3.2%). Staphylococcus isolates phenotypically resistant to methicillin also carried the mecA gene and displayed diverse pulsed field gel electrophoresis (PFGE) profiles, indicating their diverse origins in the milk supply chain. Based on the similarity of PFGE profile, the origin of one of the Staphylococcus isolates was traced to the soil in contact with milch cows. The findings of this study highlight the need for more comprehensive microbial risk analysis studies across the milk supply chain, capacity building, creation of awareness among stakeholders about the judicious use of antimicrobials, and protection of public health using a One-Health approach.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"19 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141151706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roles of mitochondrial alternative oxidase in photosynthetic electron transport in illuminated leaves of Arabidopsis thaliana at low temperature 拟南芥低温光照叶片线粒体替代氧化酶在光合电子传递中的作用

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-05-25 DOI: 10.1007/s12038-024-00446-7

Yusuke Yamada, Kuniaki Suzuki, Hana Yanagishita, Ko Noguchi

{"title":"Roles of mitochondrial alternative oxidase in photosynthetic electron transport in illuminated leaves of Arabidopsis thaliana at low temperature","authors":"Yusuke Yamada, Kuniaki Suzuki, Hana Yanagishita, Ko Noguchi","doi":"10.1007/s12038-024-00446-7","DOIUrl":"https://doi.org/10.1007/s12038-024-00446-7","url":null,"abstract":"ATP-uncoupling alternative oxidase (AOX) in the plant respiratory chain is often induced under stress conditions such as low temperature (LT). The importance of AOX in photosynthesis has been examined, and leaves having larger amounts of AOX tended to show larger decrease in photosynthetic electron transport rate (ETR) by AOX inhibition. However, the details were not clarified. Here, we used three ecotypes of Arabidopsis thaliana which differed in AOX amounts and their responses to LT, and examined whether AOX amount was related to the degree of decrease in ETR by AOX inhibition. In Tiv-0, which originates from a warmer site, grown at high temperature (HT), AOX inhibition decreased ETR, but not in the other ecotypes. LT treatment significantly increased ETR and AOX, especially in Bur-0, but AOX inhibition did not decrease ETR in LT plants of any ecotype. AOX inhibition significantly increased the non-regulated energy dissipation in photosystem II (PSII), Y(NO), and decreased the maximal quantum yield of PSII, Fv/Fm, especially in LT plants. Since AOX inhibition did not affect the parameters of PSI, AOX inhibition may directly affect the reaction center of PSII in LT plants.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"308 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141151764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0