Journal of Biosciences最新文献_第2页

Sensory modulation of neuropeptide signaling by CASY-1 gates cholinergic transmission at Caenorhabditis elegans neuromuscular junction. CASY-1对秀丽隐杆线虫神经肌肉交界处胆碱能传递的神经肽信号的感觉调节。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Navneet Shahi, Shruti Thapliyal, Kavita Babu

{"title":"Sensory modulation of neuropeptide signaling by CASY-1 gates cholinergic transmission at Caenorhabditis elegans neuromuscular junction.","authors":"Navneet Shahi, Shruti Thapliyal, Kavita Babu","doi":"","DOIUrl":"","url":null,"abstract":"The neuromuscular junction (NMJ) is crucial for understanding the fundamentals of synaptic transmission and activity. Various modulators operate within neuronal circuits, from sensory to motor neurons, to influence synaptic transmission at the NMJ. This study sheds light on the regulation of sensory-evoked cholinergic neurotransmission at motor neurons orchestrated by CASY-1, the mammalian calsyntenin orthologue. We report that the increased excitation-inhibition (E-I) ratio at the NMJ in casy-1 mutants is likely due to its interactions with neuromodulators in sensory neurons. We explored the intricate genetic interactions of CASY- 1 with the neuropeptide FLP-21 and its receptor, NPR-1, both of which display simultaneous alterations in cholinergic signaling at the NMJ. Through genetic, pharmacological, and bioimaging-based experiments, we proposed a mechanism by which CASY-1 potentially interacts with the neuropeptide-carrying vesicles to regulate synaptic transmission. The nematode Caenorhabditis elegans serves as an ideal model system for this study, enabling detailed insights into neuromodulatory mechanisms in the neuronal circuit.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7617471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmaceutical adoption of microphysiological systems in India is contingent on their economics among other aspects. 在印度，微生理系统在制药行业的应用取决于其经济效益和其他方面。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Kasturi Mahadik, Surat Parvatam, Nalam Madhusudhana Rao

引用次数: 0

Kinome-wide RNAi screening in Caenorhabditis elegans reveals new modulators of insulin signaling and longevity. 秀丽隐杆线虫的全基因组RNAi筛选揭示了胰岛素信号传导和寿命的新调节剂。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Manish Chamoli, Anna Foulger, Prachi Singh, Gordon Lithgow, Arnab Mukhopadhyay

{"title":"Kinome-wide RNAi screening in Caenorhabditis elegans reveals new modulators of insulin signaling and longevity.","authors":"Manish Chamoli, Anna Foulger, Prachi Singh, Gordon Lithgow, Arnab Mukhopadhyay","doi":"","DOIUrl":"","url":null,"abstract":"The insulin/IGF-I-like signaling (IIS) pathway is a highly conserved signaling cascade that plays a crucial role in regulating longevity across species. Given its significance in aging, identifying novel kinases interacting with the IIS pathway can provide deeper insights into the mechanisms governing longevity. In this study, we performed a targeted RNAi screening of the Caenorhabditis elegans kinome, using dauer formation as a phenotypic readout. We identified several known and novel kinase modulators of the IIS pathway. These hits were enriched with both previously documented and undocumented lifespan regulators. Thermotolerance assays revealed mixed trends, with some kinase inhibitions increasing while others decreasing protection against thermal stress. We observed a positive correlation between thermotolerance and lifespan extension, as well as between dauer formation and lifespan extension, with thermotolerance proving to be a better predictor of longevity. Our findings offer a valuable resource for researchers exploring the IIS pathway and highlight novel, unannotated kinases as potential new therapeutic targets for aging interventions.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting sequence polymorphism and structural basis patterns of Plasmodium falciparum histidine-rich proteins 2/3 in Cameroon and India. 喀麦隆和印度恶性疟原虫富组氨酸蛋白2/3序列多态性和结构基谱对比

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Loick Pradel Kojom Foko, Joseph Hawadak, Soumyananda Chakraborti, Veena Pande, Vineeta Singh

{"title":"Contrasting sequence polymorphism and structural basis patterns of Plasmodium falciparum histidine-rich proteins 2/3 in Cameroon and India.","authors":"Loick Pradel Kojom Foko, Joseph Hawadak, Soumyananda Chakraborti, Veena Pande, Vineeta Singh","doi":"","DOIUrl":"","url":null,"abstract":"The bulk of malaria rapid diagnostic tests (RDTs) target Plasmodium falciparum histidine-rich protein 2 (PfHRP2), but several reports have shown that sequence variations in this protein are associated with falsenegative RDT results. The polymorphism of PfHRP2/3 was analyzed from Cameroonian and Indian P. falciparum isolates. Cameroon and India are two of eleven countries with the highest malaria burden. Exon 2 of pfhrp 2/3 genes were PCR-amplified, and the amplicons were purified and sequenced. A total of 25 PfHRP2 and 12 PfHRP3 novel repeat type variants were found. The nature and organization of PfHRP3 sequences were quite similar between Cameroon and India. Some structurally unique PfHRP2/3 sequences, characterized by a high proportion of proline (5.8-10.3%) for PfHRP2, and two non-repeat regions for PfHRP3, were found in both countries. Most of the Cameroonian isolates belonged to group B (66.7%), while the Indian isolates belonged to group C (69.2%) (p=0.03). Three epitope motifs (AHHAHHA, HATDAHH, and YAHHAHHA) were found in all Cameroonian and Indian PfHRP2 sequences. Mutations observed in unique sequences were mainly associated with alterations of helical structures in the PfHRP2 C-terminal region. The high genetic diversity, epitope availability, and structural basis patterns found here could help develop the next generation of RDTs with improved quality.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What keeps Caenorhabditis elegans precise: The spatiotemporal regulation of defecation. 是什么让秀丽隐杆线虫保持精确：排便的时空调节。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Qiang Liu, Louis Tao

引用次数: 0

Co-chaperones fine-tune the function of heat shock protein 70 (Hsp70), whether to fold, hold, or degrade substrates in ensuring cellular protein homeostasis. 共同伴侣调节热休克蛋白70 （Hsp70）的功能，无论是折叠、保持还是降解底物，以确保细胞蛋白稳态。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Pramit Bhattacharjee, Joydeep Roy, Atin Kumar Mandal

{"title":"Co-chaperones fine-tune the function of heat shock protein 70 (Hsp70), whether to fold, hold, or degrade substrates in ensuring cellular protein homeostasis.","authors":"Pramit Bhattacharjee, Joydeep Roy, Atin Kumar Mandal","doi":"","DOIUrl":"","url":null,"abstract":"The molecular chaperone Hsp70 is a pivotal player in cellular protein quality control due to its wide range of substrates ranging from unfolded, native, to misfolded proteins. Increasing evidence suggests that Hsp70 decides the fate of proteins; however, the inherent rules that govern the decision-making capacity of Hsp70 are not clear. In this review, we have articulated the functions of Hsp70 with respect to proteostasis and established a link between its co-chaperones in deciding the fate of the substrate. The substrate binding of Hsp70 is mediated by its catalytic cycle where Hsp70 achieves high- and low-substrate-affinity ADP- and ATP-bound forms, respectively. This catalytic cycle of Hsp70 is maintained by co-chaperones J-domain proteins (JDPs), and nucleotide exchange factors (NEFs). JDPs bind to the ATP-bound form of Hsp70 and hydrolyze ATP that enhances substrate binding, whereas NEFs exchange ADP with ATP and facilitate substrate release. During evolution, several isoforms of Hsp70 and its co-chaperones have emerged which may have functional significance. Apart from facilitating the catalytic cycle of Hsp70, co-chaperones often mediate collaboration between Hsp70 and downstream protein quality-control pathways such as the ubiquitin proteasome system, autophagy, or disaggregase machinery. Therefore, co-chaperones have a significant role in Hsp70's triage decision of whether to fold, hold, or degrade.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of components of the kallikrein-kinin system in the spine epithelium of the Atlantic stingray, Hypanus sabinus. 大西洋黄貂鱼棘上皮中钾激肽-激肽系统成分的鉴定。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

E R Lacy, D H Miller, B J Wiley

{"title":"Identification of components of the kallikrein-kinin system in the spine epithelium of the Atlantic stingray, Hypanus sabinus.","authors":"E R Lacy, D H Miller, B J Wiley","doi":"","DOIUrl":"","url":null,"abstract":"The epithelial sheath covering the stingray spine results in wounds to humans that are characterized by edema, necrosis, effusive bleeding and extreme pain. Kinins are potent autocoids that produce each of these symptoms. In this study, the dorsal and ventral portions of the epithelial sheath covering the spine of the Atlantic stingray (Hypanus sabinus) spine were analysed for components of the kallikrein-kinin system. Colorimetric assays showed kallikrein activity in both dorsal and ventral epithelial sheath preparations. Trypsin, which cleaves the inactive proenzyme to its active (kallikrein) form, resulted in an increase in the median kallikrein of 2.02 and 0.94 in dorsal and ventral spine preparations, respectively. Radioimmunoassay of kinin itself showed detectable immunoreactivity in the entire integumentary sheath. Trypsin treatment resulted in an increase in median immunoreactivity by 12.88. In vivo analyses for effects of epithelial extract on mammalian capillary leakage showed an increase in median capillary leakage of 5.25 in spine epithelia-treated animals compared to controls. Components of the kallikrein-kinin system are present in the Atlantic stingray spine epithelium and may account for some of the pathologies of stings in humans.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ARP2/3 regulates cell surface dynamics of CeTOCA-1 in C. elegans zygotes. ARP2/3调控秀丽隐杆线虫受精卵中CeTOCA-1的细胞表面动力学。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Aadya Nageswaran, Promiti Mitra, Megha Rai, Diya Anand, Yogesh Pratap, Anup Padmanabhan

{"title":"ARP2/3 regulates cell surface dynamics of CeTOCA-1 in C. elegans zygotes.","authors":"Aadya Nageswaran, Promiti Mitra, Megha Rai, Diya Anand, Yogesh Pratap, Anup Padmanabhan","doi":"","DOIUrl":"","url":null,"abstract":"Polymerization of branched actin networks by the ARP2/3 complex plays a critical role in diverse cellular processes. ARP2/3 activity is tightly controlled by the upstream CDC-42 GTPase and effectors such as the Wiscott-Aldrich syndrome protein (N-WASP/Wiscott-Aldrich Syndrome Protein (WSP-1)) and members of the F-BAR containing transducer of CDC-42-dependent actin assembly (TOCA) protein family. While the mechanisms governing WASP/N-WASP (neural-WASP) functioning are well understood, the regulatory dynamics of TOCA proteins at the cell cortex remain poorly characterized. Here, using the Caenorhabditis elegans zygote as a model system, we investigated the role of cortical F-actin structures - both branched and linear - in modulating surface dynamics of CeTOCA-1, the nematode ortholog of mammalian TOCA-1. In our in silico analysis, iPTM values associated with the interaction between different domains of CeTOCA-1 and CDC-42 suggested that while the HR-1 domain is essential for this interaction, the SH3 domain is dispensable for complex formation between the two proteins. Further, we experimentally disrupted ARP2/3 and CYK-1/ formin-polymerized F-actin structures in C. elegans zygotes to examine the role of cortical F-actin on CeTOCA-1 assembly dynamics and biophysical properties. Co-localization studies revealed a preferential association between CeTOCA-1 and the pool of F-actin structures polymerized by ARP2/3. Disruption of ARP2/3 led to the formation of larger CeTOCA-1 clusters, prolonged cluster lifetime on the cell surface, and reduced cluster mobility. These findings suggest that distinct F-actin structures play specialized roles in mediating plasma membrane interactions and regulating surface dynamics of CeTOCA-1 clusters.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of exosomes derived from induced and human adipose tissue-derived mesenchymal stem cells on human cancer cells. 诱导和人脂肪组织来源的间充质干细胞外泌体对人癌细胞的影响。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Razan Aldiqs, Sura Nashwan, Mohammad A Ismail, Tareq Saleh, Raghda Barham, Malik Zihlif, Nidaa A Ababneh

{"title":"Effect of exosomes derived from induced and human adipose tissue-derived mesenchymal stem cells on human cancer cells.","authors":"Razan Aldiqs, Sura Nashwan, Mohammad A Ismail, Tareq Saleh, Raghda Barham, Malik Zihlif, Nidaa A Ababneh","doi":"","DOIUrl":"","url":null,"abstract":"Exosomes (Exos) derived from mesenchymal stem cells (MSCs) are known to influence cancer cell behavior; however, the clinical use of MSCs is limited due to the gradual loss of their differentiation potential with continuous passaging. Induced mesenchymal stem cells (iMSCs) have emerged as a promising alternative source, but the effects of Exos derived from iMSCs (iMSC-Exos) on cancer cells remain incompletely understood. This study aims to compare the effects of iMSC-Exos with ADMSC-Exos derived from adipose tissue-derived mesenchymal stem cells (ADMSCs) on the viability, invasion, and migration of breast (MCF7) and lung (A549) cancer cells. Conditioned media from iMSCs and ADMSCs were collected for isolation and characterization of Exos. MCF7 and A549 cell lines were treated with iMSC- and ADMSC-Exos, and Exos uptake, cell viability, migration, senescence, and expression of BAX and BCL-2 genes were evaluated. iMSCand ADMSC-Exos were successfully internalized into cancer cells, with a higher efficiency of ADMSC-Exos uptake in MCF7 cells. Cell viability decreased and migration increased in both cancer cell lines upon treatment. BAX expression was significantly reduced in MCF7 cells following ADMSC-Exos treatment and in A549 cells after iMSC-Exos treatment. In contrast, BCL-2 expression was significantly reduced in MCF7 cells treated with both iMSC- and ADMSC-Exos, while it significantly increased in A549 cells after ADMSC-Exos treatment. A549 lung cancer cells showed a higher level of senescence than MCF7 breast cancer cells, particularly when treated with iMSC-Exos. Minimal overall differences were observed in viability, apoptosis, and migration assays between iMSC- and ADMSC-Exos in MCF7 and A549 cells. However, significant differences were observed in the senescence and expression of BAX and BCL-2 genes across cancer cell lines. These findings highlight the importance of further investigation into the distinct effects of iMSC- and ADMSC-Exos on cancer cell biology.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors shaping resource partitioning in predators of clumped patches of bamboo woolly aphids. 竹绵蚜成片地捕食者资源分配的影响因素。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Basant Kumar Agarwala

{"title":"Factors shaping resource partitioning in predators of clumped patches of bamboo woolly aphids.","authors":"Basant Kumar Agarwala","doi":"","DOIUrl":"","url":null,"abstract":"Prey-predator interactions favor the aggregation of generalist predators adept at avoiding competition in foraging on temporary food resources. There is scant information on the predator guild on clumpy patches of woolly aphids on bamboo host plants in forested landscapes. Results of a field-cum-laboratory study explain the aggregation of generalist and specialist predators in patchy resources of the bamboo-feeding woolly aphid, Ceratovacuna silvestrii, with particular reference to the specialist giant ladybird predator, Anisolemnia dilatata. This predator's larvae share food resources for 26 weeks including 11 weeks of coexistence with 2 small-sized generalist predators and 11 weeks with the larvae of specialist moth predator. Results show a preference for low prey density patches by small-sized predators in contrast to high prey density by the giant ladybird predator. Between the two woolly aphid prey-specialist predators, the moth caterpillars (Dipha aphidivora) avoided competition with the giant ladybirds by foraging in silken nests in less aggregated aphid patches. Eggs and larvae of the giant ladybird predator are defended from heterospecific and conspecific predators. This trait deters other predators of the guild that avoid prey patches visited by the giant ladybirds. Bigger size, preference for high-density prey patches, and anti-predation trait of eggs and larvae confer selection advantages to giant ladybirds as the top predator of the guild of woolly aphids. This has evolutionary significance for the ecological stability of prey-predator dynamics in a forested landscape.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0