Journal of Biosciences最新文献_第2页

Identifying trials run in India, registered with ClinicalTrials.gov, is not straightforward. 确定在印度进行并在ClinicalTrials.gov上注册的试验并不简单。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Anwesha Dhal Samanta, Jaishree Mendiratta, Gayatri Saberwal

{"title":"Identifying trials run in India, registered with ClinicalTrials.gov, is not straightforward.","authors":"Anwesha Dhal Samanta, Jaishree Mendiratta, Gayatri Saberwal","doi":"","DOIUrl":"","url":null,"abstract":"Clinical trial registries are invaluable resources for many stakeholders. However, these registries often require customized methodologies to identify studies of interest. To determine what steps it would take to catalog every trial that has run in India, in this study, we examined the records held by the registry of the United States, ClinicalTrials.gov. We utilized the Aggregate Analysis of ClinicalTrials.gov database, which draws data from ClinicalTrials.gov. Starting with 458,069 records, a shortlist of 11,439 records of interest was identified that contained the keywords 'India' or 'CTRI', the latter standing for Clinical Trials Registry-India. The Location field indicated that India had been a country of recruitment in 1,416 records. Other, non-obvious fields indicated that 52 records were concerned with trials in India. Among these, 35 cases used confirmatory language, and 17 cases had circumstantial evidence for the studies having run in India. It ought to be easier to unambiguously determine which countries a study has run in. Addressing this challenge is crucial for ensuring the accuracy, completeness, and transparency of clinical trial data, benefiting patients, healthcare providers, researchers, regulators, policymakers, and other stakeholders.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sensory modulation of neuropeptide signaling by CASY-1 gates cholinergic transmission at Caenorhabditis elegans neuromuscular junction. CASY-1对秀丽隐杆线虫神经肌肉交界处胆碱能传递的神经肽信号的感觉调节。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Navneet Shahi, Shruti Thapliyal, Kavita Babu

{"title":"Sensory modulation of neuropeptide signaling by CASY-1 gates cholinergic transmission at Caenorhabditis elegans neuromuscular junction.","authors":"Navneet Shahi, Shruti Thapliyal, Kavita Babu","doi":"","DOIUrl":"","url":null,"abstract":"The neuromuscular junction (NMJ) is crucial for understanding the fundamentals of synaptic transmission and activity. Various modulators operate within neuronal circuits, from sensory to motor neurons, to influence synaptic transmission at the NMJ. This study sheds light on the regulation of sensory-evoked cholinergic neurotransmission at motor neurons orchestrated by CASY-1, the mammalian calsyntenin orthologue. We report that the increased excitation-inhibition (E-I) ratio at the NMJ in casy-1 mutants is likely due to its interactions with neuromodulators in sensory neurons. We explored the intricate genetic interactions of CASY- 1 with the neuropeptide FLP-21 and its receptor, NPR-1, both of which display simultaneous alterations in cholinergic signaling at the NMJ. Through genetic, pharmacological, and bioimaging-based experiments, we proposed a mechanism by which CASY-1 potentially interacts with the neuropeptide-carrying vesicles to regulate synaptic transmission. The nematode Caenorhabditis elegans serves as an ideal model system for this study, enabling detailed insights into neuromodulatory mechanisms in the neuronal circuit.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7617471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caenorhabditis elegans germline flux pulls its stem-cell niche cells into shape. 秀丽隐杆线虫的生殖系通量使其干细胞生态位细胞成形。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Priti Agarwal

引用次数: 0

From the microscopic gladiator pit: Antibiotics vs resistance. 从微观的角斗士坑：抗生素与耐药性。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Nishad Matange

引用次数: 0

Pharmaceutical adoption of microphysiological systems in India is contingent on their economics among other aspects. 在印度，微生理系统在制药行业的应用取决于其经济效益和其他方面。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Kasturi Mahadik, Surat Parvatam, Nalam Madhusudhana Rao

引用次数: 0

Kinome-wide RNAi screening in Caenorhabditis elegans reveals new modulators of insulin signaling and longevity. 秀丽隐杆线虫的全基因组RNAi筛选揭示了胰岛素信号传导和寿命的新调节剂。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Manish Chamoli, Anna Foulger, Prachi Singh, Gordon Lithgow, Arnab Mukhopadhyay

{"title":"Kinome-wide RNAi screening in Caenorhabditis elegans reveals new modulators of insulin signaling and longevity.","authors":"Manish Chamoli, Anna Foulger, Prachi Singh, Gordon Lithgow, Arnab Mukhopadhyay","doi":"","DOIUrl":"","url":null,"abstract":"The insulin/IGF-I-like signaling (IIS) pathway is a highly conserved signaling cascade that plays a crucial role in regulating longevity across species. Given its significance in aging, identifying novel kinases interacting with the IIS pathway can provide deeper insights into the mechanisms governing longevity. In this study, we performed a targeted RNAi screening of the Caenorhabditis elegans kinome, using dauer formation as a phenotypic readout. We identified several known and novel kinase modulators of the IIS pathway. These hits were enriched with both previously documented and undocumented lifespan regulators. Thermotolerance assays revealed mixed trends, with some kinase inhibitions increasing while others decreasing protection against thermal stress. We observed a positive correlation between thermotolerance and lifespan extension, as well as between dauer formation and lifespan extension, with thermotolerance proving to be a better predictor of longevity. Our findings offer a valuable resource for researchers exploring the IIS pathway and highlight novel, unannotated kinases as potential new therapeutic targets for aging interventions.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting sequence polymorphism and structural basis patterns of Plasmodium falciparum histidine-rich proteins 2/3 in Cameroon and India. 喀麦隆和印度恶性疟原虫富组氨酸蛋白2/3序列多态性和结构基谱对比

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Loick Pradel Kojom Foko, Joseph Hawadak, Soumyananda Chakraborti, Veena Pande, Vineeta Singh

{"title":"Contrasting sequence polymorphism and structural basis patterns of Plasmodium falciparum histidine-rich proteins 2/3 in Cameroon and India.","authors":"Loick Pradel Kojom Foko, Joseph Hawadak, Soumyananda Chakraborti, Veena Pande, Vineeta Singh","doi":"","DOIUrl":"","url":null,"abstract":"The bulk of malaria rapid diagnostic tests (RDTs) target Plasmodium falciparum histidine-rich protein 2 (PfHRP2), but several reports have shown that sequence variations in this protein are associated with falsenegative RDT results. The polymorphism of PfHRP2/3 was analyzed from Cameroonian and Indian P. falciparum isolates. Cameroon and India are two of eleven countries with the highest malaria burden. Exon 2 of pfhrp 2/3 genes were PCR-amplified, and the amplicons were purified and sequenced. A total of 25 PfHRP2 and 12 PfHRP3 novel repeat type variants were found. The nature and organization of PfHRP3 sequences were quite similar between Cameroon and India. Some structurally unique PfHRP2/3 sequences, characterized by a high proportion of proline (5.8-10.3%) for PfHRP2, and two non-repeat regions for PfHRP3, were found in both countries. Most of the Cameroonian isolates belonged to group B (66.7%), while the Indian isolates belonged to group C (69.2%) (p=0.03). Three epitope motifs (AHHAHHA, HATDAHH, and YAHHAHHA) were found in all Cameroonian and Indian PfHRP2 sequences. Mutations observed in unique sequences were mainly associated with alterations of helical structures in the PfHRP2 C-terminal region. The high genetic diversity, epitope availability, and structural basis patterns found here could help develop the next generation of RDTs with improved quality.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What keeps Caenorhabditis elegans precise: The spatiotemporal regulation of defecation. 是什么让秀丽隐杆线虫保持精确：排便的时空调节。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Qiang Liu, Louis Tao

引用次数: 0

Co-chaperones fine-tune the function of heat shock protein 70 (Hsp70), whether to fold, hold, or degrade substrates in ensuring cellular protein homeostasis. 共同伴侣调节热休克蛋白70 （Hsp70）的功能，无论是折叠、保持还是降解底物，以确保细胞蛋白稳态。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

Pramit Bhattacharjee, Joydeep Roy, Atin Kumar Mandal

{"title":"Co-chaperones fine-tune the function of heat shock protein 70 (Hsp70), whether to fold, hold, or degrade substrates in ensuring cellular protein homeostasis.","authors":"Pramit Bhattacharjee, Joydeep Roy, Atin Kumar Mandal","doi":"","DOIUrl":"","url":null,"abstract":"The molecular chaperone Hsp70 is a pivotal player in cellular protein quality control due to its wide range of substrates ranging from unfolded, native, to misfolded proteins. Increasing evidence suggests that Hsp70 decides the fate of proteins; however, the inherent rules that govern the decision-making capacity of Hsp70 are not clear. In this review, we have articulated the functions of Hsp70 with respect to proteostasis and established a link between its co-chaperones in deciding the fate of the substrate. The substrate binding of Hsp70 is mediated by its catalytic cycle where Hsp70 achieves high- and low-substrate-affinity ADP- and ATP-bound forms, respectively. This catalytic cycle of Hsp70 is maintained by co-chaperones J-domain proteins (JDPs), and nucleotide exchange factors (NEFs). JDPs bind to the ATP-bound form of Hsp70 and hydrolyze ATP that enhances substrate binding, whereas NEFs exchange ADP with ATP and facilitate substrate release. During evolution, several isoforms of Hsp70 and its co-chaperones have emerged which may have functional significance. Apart from facilitating the catalytic cycle of Hsp70, co-chaperones often mediate collaboration between Hsp70 and downstream protein quality-control pathways such as the ubiquitin proteasome system, autophagy, or disaggregase machinery. Therefore, co-chaperones have a significant role in Hsp70's triage decision of whether to fold, hold, or degrade.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of components of the kallikrein-kinin system in the spine epithelium of the Atlantic stingray, Hypanus sabinus. 大西洋黄貂鱼棘上皮中钾激肽-激肽系统成分的鉴定。

IF 2.1 4区生物学

Journal of Biosciences Pub Date : 2025-01-01

E R Lacy, D H Miller, B J Wiley

{"title":"Identification of components of the kallikrein-kinin system in the spine epithelium of the Atlantic stingray, Hypanus sabinus.","authors":"E R Lacy, D H Miller, B J Wiley","doi":"","DOIUrl":"","url":null,"abstract":"The epithelial sheath covering the stingray spine results in wounds to humans that are characterized by edema, necrosis, effusive bleeding and extreme pain. Kinins are potent autocoids that produce each of these symptoms. In this study, the dorsal and ventral portions of the epithelial sheath covering the spine of the Atlantic stingray (Hypanus sabinus) spine were analysed for components of the kallikrein-kinin system. Colorimetric assays showed kallikrein activity in both dorsal and ventral epithelial sheath preparations. Trypsin, which cleaves the inactive proenzyme to its active (kallikrein) form, resulted in an increase in the median kallikrein of 2.02 and 0.94 in dorsal and ventral spine preparations, respectively. Radioimmunoassay of kinin itself showed detectable immunoreactivity in the entire integumentary sheath. Trypsin treatment resulted in an increase in median immunoreactivity by 12.88. In vivo analyses for effects of epithelial extract on mammalian capillary leakage showed an increase in median capillary leakage of 5.25 in spine epithelia-treated animals compared to controls. Components of the kallikrein-kinin system are present in the Atlantic stingray spine epithelium and may account for some of the pathologies of stings in humans.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"50 ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0