Journal of Biosciences最新文献_第2页

Defining genomic landscape for identification of potential candidate resistance genes associated with major rice diseases through MetaQTL analysis 通过元 QTL 分析确定与水稻主要病害相关的潜在候选抗性基因的基因组图谱

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-07-23 DOI: 10.1007/s12038-024-00460-9

Simran Goyal, Dinesh Kumar Saini, Pankaj Kumar, Gurwinder Kaur, Umesh Preethi Praba, Krishna Sai Karnatam, Gautam Chhabra, Rajveer Singh, Yogesh Vikal

{"title":"Defining genomic landscape for identification of potential candidate resistance genes associated with major rice diseases through MetaQTL analysis","authors":"Simran Goyal, Dinesh Kumar Saini, Pankaj Kumar, Gurwinder Kaur, Umesh Preethi Praba, Krishna Sai Karnatam, Gautam Chhabra, Rajveer Singh, Yogesh Vikal","doi":"10.1007/s12038-024-00460-9","DOIUrl":"https://doi.org/10.1007/s12038-024-00460-9","url":null,"abstract":"Rice production is severely affected by various diseases such as bacterial leaf blight (BLB), brown spot (BS), false smut (FS), foot rot (FR), rice blast (RB), and sheath blight (SB). In recent years, several quantitative trait loci (QTLs) studies involving different populations have been carried out, resulting in the identification of hundreds of resistance QTLs for each disease. These QTLs can be integrated and analyzed using meta-QTL (MQTL) analysis for better understanding of the genetic architecture underlying multiple disease resistance (MDR). This study involved an MQTL analysis on 661 QTLs (378, 161, 21, 41, 44, and 16 QTLs for SB, RB, BLB, BS, FS, and FR, respectively) retrieved from 50 individual studies published from 1995 to 2021. Of these, 503 QTLs were projected finally onto the consensus map saturated with 6,275 markers, resulting in 73 MQTLs, including 27 MDR-MQTLs conferring resistance to three or more diseases. Forty-seven MQTLs were validated using marker-trait associations identified in published genome-wide association studies. A total of 3,310 genes, including both R and defense genes, were also identified within some selected high-confidence MQTL regions that were investigated further for the syntenic relationship with barley, wheat, and maize genomes. Thirty-nine high-confidence candidate genes were selected based on their expression patterns and recommended for future studies involving functional validation, genetic engineering, and gene editing. Nineteen MQTLs were co-localized with 39 known R genes for BLB and RB diseases. These results could pave the way to utilize candidate genes in a marker-assisted breeding program for MDR in rice.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"32 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141770885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trehalase inhibition in Helicoverpa armigera activates machinery for alternate energy acquisition 抑制 Helicoverpa armigera 中的 Trehalase 可激活替代能量获取机制

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-07-18 DOI: 10.1007/s12038-024-00453-8

Meenakshi Tellis, Sharada Mohite, Rakesh Joshi

{"title":"Trehalase inhibition in Helicoverpa armigera activates machinery for alternate energy acquisition","authors":"Meenakshi Tellis, Sharada Mohite, Rakesh Joshi","doi":"10.1007/s12038-024-00453-8","DOIUrl":"https://doi.org/10.1007/s12038-024-00453-8","url":null,"abstract":"Trehalose serves as a primary circulatory sugar in insects which is crucial in energy metabolism and stress recovery. It is hydrolyzed into two glucose molecules by trehalase. Silencing or inhibiting trehalase results in reduced fitness, developmental defects, and insect mortality. Despite its importance, the molecular response of insects to trehalase inhibition is not known. Here, we performed transcriptomic analyses of Helicoverpa armigera treated with validamycin A (VA), a trehalase inhibitor. VA ingestion resulted in increased mortality, developmental delay, and reduced ex vivo trehalase activity. Pathway enrichment and gene ontology analyses suggest that key genes involved in carbohydrate, protein, fatty acid, and mitochondria-related metabolisms are deregulated. The activation of protein and fat degradation may be necessary to fulfil energy requirements, evidenced by the dysregulated expression of critical genes in these metabolisms. Co-expression analysis supports the notion that trehalase inhibition leads to putative interaction with key regulators of other pathways. Metabolomics correlates with transcriptomics to show reduced levels of key energy metabolites. VA generates an energy-deficient condition, and insects activate alternate pathways to facilitate the energy demand. Overall, this study provides insights into the molecular mechanisms underlying the response of insects to trehalase inhibition and highlights potential targets for insect control.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"61 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141740144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Goat weed (Ageratum conyzoides L.): A biological threat to plant diversity in Eastern Ghats of India 山羊草（Ageratum conyzoides L.）：对印度东高止山植物多样性的生物威胁

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-07-10 DOI: 10.1007/s12038-024-00455-6

Prakash Paraseth, Kakoli Banerjee

{"title":"Goat weed (Ageratum conyzoides L.): A biological threat to plant diversity in Eastern Ghats of India","authors":"Prakash Paraseth, Kakoli Banerjee","doi":"10.1007/s12038-024-00455-6","DOIUrl":"https://doi.org/10.1007/s12038-024-00455-6","url":null,"abstract":"The Kunming–Montreal Global Biodiversity Framework (GBF) is a recently signed protocol by the conference of the parties (COP 15) with an aim to protect biodiversity from risks imposed by biological threats such as invasive alien species (IAS). The present work is an effort to meet target 6 of GBF which directly deals with IAS by assessing the current and future distribution of Ageratum species in regions of the Eastern Ghats of India. Prediction of Ageratum distribution was done based on greenhouse gas emission levels, namely RCP 4.5, 6.0 and 8.5 for the climatic years 2030, 2050 and 2080. Of a total of 23 environmental parameters (19 bioclimatic, 1 land use land cover (LULC) and 3 topographic) seven were selected for species distribution modeling (SDM) considering value inflation factor (VIF) scores <3 by using maximum entropy. In the current climatic scenario, 40.09% of the geographical area (TGA) is covered by Ageratum species which will reach 76.51%, 77.44%, 82.58% for RCP 4.5, 6.0 and 8.5 respectively by the end of 2100. Both the AUC value (0.884) and Jackknife test have shown a good model performance. The Eastern Ghats, being a biodiversity-rich zone, needs efficient conservation and management strategies to decrease the extent of invaded areas to maximize biodiversity returns.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"25 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141569606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide brings innate immune resistance to M. tuberculosis infection among high-risk household contacts of pulmonary tuberculosis patients 一氧化氮使肺结核患者的高危家庭接触者对结核杆菌感染产生先天免疫抵抗力

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-07-10 DOI: 10.1007/s12038-024-00459-2

Sudhasini Panda, Ambrish Tiwari, Kalpana Luthra, Kuldeep Kumar, Archana Singh

{"title":"Nitric oxide brings innate immune resistance to M. tuberculosis infection among high-risk household contacts of pulmonary tuberculosis patients","authors":"Sudhasini Panda, Ambrish Tiwari, Kalpana Luthra, Kuldeep Kumar, Archana Singh","doi":"10.1007/s12038-024-00459-2","DOIUrl":"https://doi.org/10.1007/s12038-024-00459-2","url":null,"abstract":"Nitric oxide (NO) and iNOS are crucial host factors in innate immunity against intracellular pathogens. However, the role of NO in Mycobacterium tuberculosis (M. tb) infection in humans remains controversial, unlike in the murine model of TB. To investigate this, levels of NO, iNOS, and L-arginine, as well as the NOS2A gene polymorphism rs57234985 at the promoter region of NOS2A, were evaluated in pulmonary TB (PTB) patients and their household contacts (HHCs). Increased levels of NO and iNOS expression in HHCs indicated exposure to M. tb infection which was confirmed by higher levels of iNOS and NO in Mantoux-positive individuals. Furthermore, higher levels of arginine were detected in HHCs, suggesting its potential role in promoting optimal NO synthesis. PTB patients had higher levels of these analytes due to ongoing active infection. Interestingly, iNOS and NO levels were inversely related to bacterial burden, suggesting their antimicrobial role. NOS2A gene polymorphism was found to be associated with disease susceptibility, with the TT genotype linked to increased iNOS expression. To conclude, iNOS plays a crucial role in controlling early M. tb infection in HHCs by inducing optimal NO production with help of L-arginine. Further longitudinal studies are needed to better understand the role of these host factors upon disease activation.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"83 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141569607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell membrane proteome analysis in HEK293T cells challenged with α-synuclein amyloids 用α-突触核蛋白淀粉样蛋白挑战 HEK293T 细胞的细胞膜蛋白质组分析

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-07-05 DOI: 10.1007/s12038-024-00457-4

Harshit Vaish, Shemin Mansuri, Aanchal Jain, Swasti Raychaudhuri

{"title":"Cell membrane proteome analysis in HEK293T cells challenged with α-synuclein amyloids","authors":"Harshit Vaish, Shemin Mansuri, Aanchal Jain, Swasti Raychaudhuri","doi":"10.1007/s12038-024-00457-4","DOIUrl":"https://doi.org/10.1007/s12038-024-00457-4","url":null,"abstract":"Amyloids interact with plasma membranes. Extracellular amyloids cross the plasma membrane barrier. Internalized extracellular amyloids are reported to trigger amyloidogenesis of endogenous proteins in recipient cells. To what extent these extracellular and intracellular amyloids perturb the plasma membrane proteome is not investigated. Using α-synuclein as a model amyloid protein, we performed membrane shaving followed by mass spectrometry experiments to identify the conformational changes in cell surface proteins after extracellular amyloid challenge. We also performed membrane proteomics after the biogenesis of intracellular α-synuclein amyloids. Our results suggest that promiscuous interactions with extracellular amyloids stochastically alter the conformation of plasma membrane proteins. This affects the biological processes through the plasma membrane and results in loss of cell viability. Cells that survive the extracellular amyloid shock can grow normally and gradually develop intracellular amyloids which do not directly impact the plasma membrane proteome and associated biological processes. Thus, our results suggest that α-synuclein amyloids can damage the plasma membrane and related processes during cell-to-cell transfer and not during their intracellular biogenesis.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"37 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141547708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neural pathways that compel us to scratch an itch 迫使我们抓痒的神经通路

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-07-05 DOI: 10.1007/s12038-024-00452-9

Jagat Narayan Prajapati, Prannay Reddy, Arnab Barik

引用次数: 0

Comparative assessment of two in-house-built isothermal assays for visual detection of African swine fever virus 用于目测检测非洲猪瘟病毒的两种内部自制等温测定法的比较评估

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-06-28 DOI: 10.1007/s12038-024-00451-w

Gyanendra Singh Sengar, Soumendu Chakravarti, Rajib Deb, Seema Rani Pegu, Pranav Anjaria, Joyshikh Sonowal, Swaraj Rajkhowa, Pranab Joyti Das, Vivek Kumar Gupta

{"title":"Comparative assessment of two in-house-built isothermal assays for visual detection of African swine fever virus","authors":"Gyanendra Singh Sengar, Soumendu Chakravarti, Rajib Deb, Seema Rani Pegu, Pranav Anjaria, Joyshikh Sonowal, Swaraj Rajkhowa, Pranab Joyti Das, Vivek Kumar Gupta","doi":"10.1007/s12038-024-00451-w","DOIUrl":"https://doi.org/10.1007/s12038-024-00451-w","url":null,"abstract":"Owing to the lack of effective vaccines, current control measures and eradication strategies for the African swine fever virus (ASFV) rely on early detection and stringent stamping-out procedures. In the present study, we developed two independent isothermal amplification assays, namely, loop-mediated isothermal amplification (LAMP) and polymerase spiral reaction (PSR), for quick visualization of the ASFV genome in clinical samples. Additionally, a quantitative real-time PCR (qRT-PCR)-based hydrolysis probe assay was developed for comparative assessment of sensitivity with the developed isothermal assays. The analytical sensitivity of the LAMP, PSR, and qRT-PCR was found to be 2.64 ×105 copies/µL, 2.64 ×102 copies/µL, and 2.64 ×101 copies/µL, respectively. A total of 165 clinical samples was tested using the developed visual assays. The relative accuracy, relative specificity, and relative diagnostic sensitivity for LAMP vs PSR were found to be 95.37% vs 102.48%, 97.46% vs 101.36%, and 73.33% vs 113.33%, respectively.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"147 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the propensity of various genetic disorders and syndromes in the Koraga, an aboriginal tribe from southern India 揭示印度南部土著部落 Koraga 中各种遗传疾病和综合征的倾向

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-06-22 DOI: 10.1007/s12038-024-00458-3

Swathy M Krishna, Suneetha M Prabhu, Ranajit Das

{"title":"Unraveling the propensity of various genetic disorders and syndromes in the Koraga, an aboriginal tribe from southern India","authors":"Swathy M Krishna, Suneetha M Prabhu, Ranajit Das","doi":"10.1007/s12038-024-00458-3","DOIUrl":"https://doi.org/10.1007/s12038-024-00458-3","url":null,"abstract":"Koragas, recognized as a particularly vulnerable tribal group (PVTG) by the Government of India, are from coastal Karnataka and Kerala. They are experiencing severe socioeconomic and health-related issues and rapid depopulation. The unique genetic makeup of Koragas has been maintained by the practice of endogamy. We aimed to identify genetic factors potentially associated with the predisposition of Koragas towards genetic and multifactorial disorders. We employed genome-wise data of 29 Koraga individuals genotyped on the Infinium Global Screening Array-24 v3.0 BeadChip platform and performed various population genetic analyses including kinship, identity by descent (IBD), and runs of homozygosity (RoH). A high degree of haplotype sharing among the Koraga participants may be indicative of a recent founder event. We identified genetic variants and genes associated with several genetic disorders, higher infant mortality rate, neurological disorders, deafness, and lower fertility rate of this agrarian tribe. Ours is the first genome-wide study on the Koraga tribe that identified genetic factors associated with various genetic disorders. Our findings can provide public healthcare providers with essential genetic information that can be useful in augmenting medical and healthcare services and improving the quality of life of Koragas.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"6 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141530158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Collaborative action between noradrenergic and serotoninergic systems in peripheral antinociception in mice 去甲肾上腺素能系统和血清素能系统在小鼠外周抗痛觉中的协同作用

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-06-19 DOI: 10.1007/s12038-024-00461-8

Danielle Diniz Aguiar, Cristina da Costa Oliveira, Júlia Alvarenga Petrocchi, Marina Gomes Miranda E Castor, Andrea Castro Perez, Igor Dimitri Gama Duarte, Thiago Roberto Lima Romero

{"title":"Collaborative action between noradrenergic and serotoninergic systems in peripheral antinociception in mice","authors":"Danielle Diniz Aguiar, Cristina da Costa Oliveira, Júlia Alvarenga Petrocchi, Marina Gomes Miranda E Castor, Andrea Castro Perez, Igor Dimitri Gama Duarte, Thiago Roberto Lima Romero","doi":"10.1007/s12038-024-00461-8","DOIUrl":"https://doi.org/10.1007/s12038-024-00461-8","url":null,"abstract":"Noradrenaline (NA) and serotonin (5-HT) induce nociception and antinociception. This antagonistic effect can be explained by the dose and type of activated receptors. We investigated the existence of synergism between the noradrenergic and serotonergic systems during peripheral antinociception. The paw pressure test was performed in mice that had increased sensitivity by intraplantar injection of prostaglandin E2 (PGE2). Noradrenaline (80 ng) administered intraplantarly induced an antinociceptive effect, that was reversed by the administration of selective antagonists of serotoninergic receptors 5-HT1B isamoltan, 5-HT1D BRL15572, 5-HT2A ketanserin, 5-HT3 ondansetron, but not by selective receptor antagonist 5-HT7 SB-269970. The administration of escitalopram, a serotonin reuptake inhibitor, potentiated the antinociceptive effect at a submaximal dose of NA. These results, indicate the existence of synergism between the noradrenergic and serotonergic systems in peripheral antinociception in mice.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"44 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative analysis of fibrillar collagen organization in the immediate proximity of embedded fibroblasts in 3D collagen hydrogels 对三维胶原水凝胶中嵌入成纤维细胞附近的纤维胶原组织进行定量分析

IF 2.9 4区生物学

Journal of Biosciences Pub Date : 2024-06-17 DOI: 10.1007/s12038-024-00449-4

Shaunak Kanade, Milie Desai, Neel Bhatavadekar, Nagaraj Balasubramanian

{"title":"Quantitative analysis of fibrillar collagen organization in the immediate proximity of embedded fibroblasts in 3D collagen hydrogels","authors":"Shaunak Kanade, Milie Desai, Neel Bhatavadekar, Nagaraj Balasubramanian","doi":"10.1007/s12038-024-00449-4","DOIUrl":"https://doi.org/10.1007/s12038-024-00449-4","url":null,"abstract":"Fibroblasts embedded in a 3D matrix microenvironment can remodel the matrix to regulate cell adhesion and function. Collagen hydrogels are a useful in vitro system to study cell–matrix interactions in a 3D microenvironment. While major matrix reorganizations are easily recognizable, subtle changes in response to environmental or biochemical cues are challenging to discern in 3D hydrogels. Three-dimensional collagen gels at 1.0 mg/ml vs 1.5 mg/ml were labelled with DQ-collagen and imaged by confocal reflectance microscopy to evaluate these small changes. An image analysis pipeline was developed, hydrogel area and number of cross-sections analysed were optimized, and fibrillar collagen properties (number of branches, number of junctions, and average branch length) were quantified. While no significant changes were seen in fibrillar collagen organization between 1.0 mg/ml and 1.5 mg/ml collagen hydrogels, embedded mouse fibroblasts caused a significant increase in collagen branching and organization. Using the phalloidin-labelled cells, this change was quantitated in immediate proximity of the cell. A distinct increase in branch and junction numbers was observed, significantly altered by small changes in collagen concentration (1.0 mg/ml vs 1.5 mg/ml). Together, this analysis gives a quantitative evaluation of how cells respond to and modify their immediate microenvironment in a 3D collagen hydrogel.","PeriodicalId":15171,"journal":{"name":"Journal of Biosciences","volume":"20 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141511027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0