喀麦隆和印度恶性疟原虫富组氨酸蛋白2/3序列多态性和结构基谱对比

IF 2.1 4区 生物学 Q2 BIOLOGY
Journal of Biosciences Pub Date : 2025-01-01
Loick Pradel Kojom Foko, Joseph Hawadak, Soumyananda Chakraborti, Veena Pande, Vineeta Singh
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引用次数: 0

摘要

大部分疟疾快速诊断检测(RDTs)的目标是恶性疟原虫富组氨酸蛋白2 (PfHRP2),但一些报告表明,该蛋白的序列变异与恶性疟原虫快速诊断检测结果的假阴性有关。对喀麦隆和印度恶性疟原虫分离株PfHRP2/3的多态性进行了分析。喀麦隆和印度是疟疾负担最重的11个国家中的两个。对pfhrp 2/3基因外显子2进行pcr扩增,纯化扩增子并测序。共发现25个PfHRP2和12个PfHRP3新的重复型变异。喀麦隆和印度的PfHRP3序列的性质和组织结构非常相似。在这两个国家都发现了一些结构独特的PfHRP2/3序列,其特征是PfHRP2的脯氨酸比例高(5.8-10.3%),PfHRP3有两个非重复区域。大多数喀麦隆分离株属于B群(66.7%),印度分离株属于C群(69.2%)(p=0.03)。在喀麦隆和印度的PfHRP2序列中均发现3个表位基序(AHHAHHA、HATDAHH和YAHHAHHA)。在独特序列中观察到的突变主要与PfHRP2 c端区域螺旋结构的改变有关。研究发现的高遗传多样性、表位可用性和结构基础模式有助于开发质量更高的下一代rdt。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Contrasting sequence polymorphism and structural basis patterns of Plasmodium falciparum histidine-rich proteins 2/3 in Cameroon and India.

The bulk of malaria rapid diagnostic tests (RDTs) target Plasmodium falciparum histidine-rich protein 2 (PfHRP2), but several reports have shown that sequence variations in this protein are associated with falsenegative RDT results. The polymorphism of PfHRP2/3 was analyzed from Cameroonian and Indian P. falciparum isolates. Cameroon and India are two of eleven countries with the highest malaria burden. Exon 2 of pfhrp 2/3 genes were PCR-amplified, and the amplicons were purified and sequenced. A total of 25 PfHRP2 and 12 PfHRP3 novel repeat type variants were found. The nature and organization of PfHRP3 sequences were quite similar between Cameroon and India. Some structurally unique PfHRP2/3 sequences, characterized by a high proportion of proline (5.8-10.3%) for PfHRP2, and two non-repeat regions for PfHRP3, were found in both countries. Most of the Cameroonian isolates belonged to group B (66.7%), while the Indian isolates belonged to group C (69.2%) (p=0.03). Three epitope motifs (AHHAHHA, HATDAHH, and YAHHAHHA) were found in all Cameroonian and Indian PfHRP2 sequences. Mutations observed in unique sequences were mainly associated with alterations of helical structures in the PfHRP2 C-terminal region. The high genetic diversity, epitope availability, and structural basis patterns found here could help develop the next generation of RDTs with improved quality.

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来源期刊
Journal of Biosciences
Journal of Biosciences 生物-生物学
CiteScore
5.80
自引率
0.00%
发文量
83
审稿时长
3 months
期刊介绍: The Journal of Biosciences is a quarterly journal published by the Indian Academy of Sciences, Bangalore. It covers all areas of Biology and is the premier journal in the country within its scope. It is indexed in Current Contents and other standard Biological and Medical databases. The Journal of Biosciences began in 1934 as the Proceedings of the Indian Academy of Sciences (Section B). This continued until 1978 when it was split into three parts : Proceedings-Animal Sciences, Proceedings-Plant Sciences and Proceedings-Experimental Biology. Proceedings-Experimental Biology was renamed Journal of Biosciences in 1979; and in 1991, Proceedings-Animal Sciences and Proceedings-Plant Sciences merged with it.
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