Role of public DNA-Seq data in mitogenomics research: A case study on the flying fish family (Beloniformes: Exocoetidae).

IF 2.1 4区 生物学 Q2 BIOLOGY
Journal of Biosciences Pub Date : 2025-01-01
Iuri Batista DA Silva, Igor Henrique Rodrigues-Oliveira, Karine Frehner Kavalco, Fabiano Bezerra Menegidio, Rubens Pasa
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引用次数: 0

Abstract

Flying fishes (Exocoetidae) are a highly specialized group of Beloniformes, with 4 subfamilies, 7 genera, and 78 species. However,only a small number of species of flying fishes have the mitochondrial genome described sof ar. Considering the importance of mitogenomes in evolution, in this study we aimed to expand the description of mitochondrial genomes and the phylogenetic relationships of Exocoetidae. We used publicly available DNA-Seq libraries to assemble the mitochondrial genomes of 7 species of flying fishes. The mitochondrial genomes of Exocoetidae showed conserved features among all species. With 22 tRNAs, 2 rRNAs, and 13 protein-coding genes, they share common features among vertebrates.Cheilopogon was reconstructedaspolyphyletic, with four clades, which reinforces previous studies that recovered Cheilopogon as non-monophyletic.Werecoveredallfour subfamilies as monophyletic,with Parexocoetinae as the sister group of all other subfamilies.The genetic distance between Exocoetidae species was two times smaller than the genetic distance among the Hyporhamphus species, indicating a low genetic divergence in the family. Hereby, this study expanded the knowledge of mitochondrial genome features and presented one of the most comprehensive mitochondrial genome datasets used for studying flying fishestodate. This was achievable using publicly available data, reinforcing the importanceof the re-use of such data in the fields of mitogenomics and phylogenetics.

公开DNA-Seq数据在有丝分裂基因组学研究中的作用:以飞鱼科(飞鱼目:外鱼科)为例。
飞鱼(外飞鱼科)是飞鱼目中高度特化的一类,有4亚科7属78种。考虑到有丝分裂基因组在进化中的重要性,本研究旨在扩大线粒体基因组的描述和外coetidae的系统发育关系。我们利用公开的DNA-Seq文库对7种飞鱼的线粒体基因组进行了组装。外cotidae线粒体基因组在所有物种中均表现出保守特征。它们有22个trna, 2个rrna和13个蛋白质编码基因,在脊椎动物中具有共同的特征。将长臂猿重建为多系的,有四个支系,这加强了之前将长臂猿恢复为非单系的研究。我们发现所有四个亚科都是单系的,Parexocoetinae是所有其他亚科的姐妹群。外cotidae种间的遗传距离比Hyporhamphus种间的遗传距离小2倍,表明该科的遗传分化程度较低。因此,本研究扩展了线粒体基因组特征的知识,并提供了用于研究飞鱼的最全面的线粒体基因组数据集之一。这可以通过使用公开可用的数据来实现,这加强了在有丝分裂基因组学和系统发育学领域重复使用这些数据的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Biosciences
Journal of Biosciences 生物-生物学
CiteScore
5.80
自引率
0.00%
发文量
83
审稿时长
3 months
期刊介绍: The Journal of Biosciences is a quarterly journal published by the Indian Academy of Sciences, Bangalore. It covers all areas of Biology and is the premier journal in the country within its scope. It is indexed in Current Contents and other standard Biological and Medical databases. The Journal of Biosciences began in 1934 as the Proceedings of the Indian Academy of Sciences (Section B). This continued until 1978 when it was split into three parts : Proceedings-Animal Sciences, Proceedings-Plant Sciences and Proceedings-Experimental Biology. Proceedings-Experimental Biology was renamed Journal of Biosciences in 1979; and in 1991, Proceedings-Animal Sciences and Proceedings-Plant Sciences merged with it.
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