Journal of biotechnology & biomaterials最新文献_第5页

Use of Agro Industrial Residues for the Production of Amylase by Penicillium sp. for Applications in Food Industry 利用农业工业残留物生产淀粉酶的青霉菌在食品工业中的应用

Journal of biotechnology & biomaterials Pub Date : 2017-05-05 DOI: 10.4172/2155-952X.1000256

N. Arora, Supreet Kaur, Sawinder Kaur

{"title":"Use of Agro Industrial Residues for the Production of Amylase by Penicillium sp. for Applications in Food Industry","authors":"N. Arora, Supreet Kaur, Sawinder Kaur","doi":"10.4172/2155-952X.1000256","DOIUrl":"https://doi.org/10.4172/2155-952X.1000256","url":null,"abstract":"Glucoamylase production has been investigated by solid state fermentation using agro-industrial residues, rice bran, wheat bran and banana peel by Penicillium sp. Out of the different agricultural residues tested for enzyme production, rice bran yielded the maximum glucoamylase activity (10.29 ± 0.07 Units/ml), protein content (1.22 ± 0.0014 mg/ml) and specific activity (8.4 Units/mg protein) with Penicillium sp. after 7th day of fermentation as compared to other agro residues. Media supplementation with carbon and nitrogen sources enhances the enzyme activity. For maximum production of enzyme, sucrose (13.70 ± 0.77 Units/ml) (1% mass level) and yeast extract (14.41 ± 0.07Units/ml) (1% mass level) as carbon and nitrogen source respectively were found optimum on rice bran with Penicillium sp. Optimum enzyme activity was observed at 90°C, pH 9. The partial purification of enzyme from rice bran by Penicillium sp. was done with 60% ammonium sulphate precipitation showed maximum enzyme activity. Enzyme hydrolysis showed maximum activity (3.69 Units/ml) with potato starch from rice bran by Penicillium sp.","PeriodicalId":15156,"journal":{"name":"Journal of biotechnology & biomaterials","volume":"18 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81801716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Modeling of Encapsulation of Alanine Amino Acid inside a CarbonNanotube 丙氨酸氨基酸在碳纳米管内封装的建模

Journal of biotechnology & biomaterials Pub Date : 2017-05-05 DOI: 10.4172/2155-952X.1000255

Hakim AL Garalleh, Mazen Garalehab

{"title":"Modeling of Encapsulation of Alanine Amino Acid inside a CarbonNanotube","authors":"Hakim AL Garalleh, Mazen Garalehab","doi":"10.4172/2155-952X.1000255","DOIUrl":"https://doi.org/10.4172/2155-952X.1000255","url":null,"abstract":"Carbon nanotubes play a significant role in facilitating and controlling the transportation of drugs and biomolecules through their internal and external surfaces. Carbon nanotubes are also selective nano-devices because of their outstanding properties and huge potential use in many bio-medical and drug delivery applications. The proposed model aims to investigate the encapsulation of Alanine molecule inside a single-walled carbon nanotube, and to determine the minimum energy arising from the Alanine interacting with single-walled carbon nanotubes with variant radius r. We consider two possible structures as models of Alanine amino acid which are a spherical shell and discrete configuration modelled as comprising three components: the linear molecule, cylindrical group and CH3 molecule as a sphere, all interacting with infinite cylindrical single-walled carbon nanotube. The adsorption of Alanine amino acid and magnitude of total energy for each orientation calculated based on the nanotube radius r and the orientation angle φ which the amino acid makes with central axis of the cylindrical nanotube. Our results indicate that the Alanine molecule encapsulated inside the nanotubes of radius greater than 3.75 A, which is in excellent agreement with recent findings.","PeriodicalId":15156,"journal":{"name":"Journal of biotechnology & biomaterials","volume":"31 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2017-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84989204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Science and technology of multifunctional Ultrananocrystalline Diamond (UNCDTM) coatings and applications to a new generation of implantable medical devices 多功能超晶金刚石(UNCDTM)涂层的科学技术及其在新一代植入式医疗器械中的应用

Journal of biotechnology & biomaterials Pub Date : 2017-04-05 DOI: 10.4172/2155-952X.C1.072

Orl, O. Auciello

引用次数: 0

Structure-guided design of selective matrix metalloproteinase (MMP) inhibitors and their application in animal models of multiple sclerosis, sepsis, and osteoarthritis 选择性基质金属蛋白酶(MMP)抑制剂的结构引导设计及其在多发性硬化症、败血症和骨关节炎动物模型中的应用

Journal of biotechnology & biomaterials Pub Date : 2017-03-31 DOI: 10.4172/2155-952X.C1.069

G. Fields

引用次数: 0

Interaction Effects of 6-Benzylaminopurine, Indole-3-Butyricacid andUrea Fertilizer to Enhance Tillering Potential of Sugarcane (Sacharumofficinarum L) Plants 6-苄基氨基嘌呤、吲哚-3-丁酸和尿素对提高甘蔗分蘖潜力的互作效应

Journal of biotechnology & biomaterials Pub Date : 2017-03-27 DOI: 10.4172/2155-952X.1000254

Gezahegn Terefe, B. Tolera

{"title":"Interaction Effects of 6-Benzylaminopurine, Indole-3-Butyricacid andUrea Fertilizer to Enhance Tillering Potential of Sugarcane (Sacharumofficinarum L) Plants","authors":"Gezahegn Terefe, B. Tolera","doi":"10.4172/2155-952X.1000254","DOIUrl":"https://doi.org/10.4172/2155-952X.1000254","url":null,"abstract":"Even though tillering is a key yield attribute in sugarcane and determined by the genetic base of the genotype, it can also be affected by applications of plant growth regulators, phenolic compounds, nitrogen fertilizers and other agronomic management practices. With this view, the effects of BAP, IBA and Urea fertilizer on two sugarcane genotypes with early maturing and high sugar content but shy in tillering followed by few millable stalk populations and hence low in cane and sugar yield was investigated. Accordingly, three levels of BAP (0, 0.03 & 0.06 mg/l), three levels of IBA (0, 0.02 and 0.03 mg/l) and four levels of Urea (0, 300, 450 and 700 kg/ha) with two sugarcane genotypes in a completely randomized block design with 3*3*4*2 factorial treatment combination arrangements was tested. Data on the number of tillers per shoot, average shoot length (cm) and number of active leaves per shoot were collected after 60 days of planting on Luvisol of Metahara Sugar Estate. Analysis of variance proved that the interaction effects of BAP* IBA*Urea*Sugarcane genotypes was highly significant (P<0.001) on the response variables tested. In sugarcane genotype C132-81, the optimum number of tillers per shoot (11.43) was obtained at 0.03 mg/l IBA, 0.06 mg/l BAP and 300 kg/l Urea fertilizer with 30.47 cm average shoot length and 8.27 active leaves per shoot while C86-56 produced 10.97 tillers per shoot with 33 cm average shoot length and 6.57 active leaves per shoot on the same treatment combination. From this result, it can be deduced that, it is possible to double the key yield attribute; number of tillers per shoot in shy tillering sugarcane genotypes that can in turn increase cane and sugar yield.","PeriodicalId":15156,"journal":{"name":"Journal of biotechnology & biomaterials","volume":"64 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89607150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Carbon and Gold Ion Implanted Surfaces on Neuronal StemCellsÃ¢ÂÂ Functions 碳和金离子注入表面对神经元StemCellsÃ¢ÂÂ功能的影响

Journal of biotechnology & biomaterials Pub Date : 2017-02-03 DOI: 10.4172/2155-952X.1000253

Emel Sokullu, Taner DaÄcÄ, O. Gözen, Fulya Ersoy, A. Oztarhan

{"title":"The Effects of Carbon and Gold Ion Implanted Surfaces on Neuronal StemCellsÃ¢ÂÂ Functions","authors":"Emel Sokullu, Taner DaÄcÄ, O. Gözen, Fulya Ersoy, A. Oztarhan","doi":"10.4172/2155-952X.1000253","DOIUrl":"https://doi.org/10.4172/2155-952X.1000253","url":null,"abstract":"Biomaterials have been used in medicine for decades to improve the functions of tissues and organs. They are also used as prosthesis and implants which are designed to substitute functions of a lacking organ or tissue. Carbon (C) and Gold (Au) were particularly chosen due to their biocompatibility and applied as implants for decades. Carbon and gold were great ion sources for medical applications, as well. In this study, polystyrene dishes were modified using gold and carbon ions via ion implantation technique. Using this surface modification method, it was aimed to improve surface characteristics and achieve a bioactive surface for neural stem cells. Even though the integration of stem cells was promising, neural stem cell studies still have many milestones to reach. Neuro-regeneration was the most desired function for people who suffer from neural system diseases. Changing surface characteristics of scaffolds was a way to promote regeneration and ion implantation was one of the methods to modify surface properties which play a huge role in enhancing the proliferation and integration of cells. In this study, it was observed that the ion implantation stimulated the neural proliferation and the implantation of different ions on cell culture surfaces was essential to determine the effects of this technique on adhesion, proliferation, differentiation and apoptosis properties of cells in details.","PeriodicalId":15156,"journal":{"name":"Journal of biotechnology & biomaterials","volume":"5 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83364204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transposon Induced Nitrogenase in Rhizobium japonicum Infecting Vignaradiata 侵染Vignaradiata的日本根瘤菌转座子诱导的氮酶

Journal of biotechnology & biomaterials Pub Date : 2017-02-01 DOI: 10.4172/2155-952X.1000252

Abhay Ghatage, P. Rachna, Ey, N. Nawani, M. Khetmalas

引用次数: 1

Hyperdry Human Amniotic Membrane (HD-AM) is Supporting AciclovirIncluded Device of Poly-N-p-Vinyl-Benzyl-D-Lactonamide (PVLA) Spherefor Treatment of HSV-1 Infected Rabbit Keratitis Model 超干燥人羊膜(HD-AM)支持含aciclovir的聚n -p-乙烯基苄基- d -内酯酰胺(PVLA)球治疗HSV-1感染兔角膜炎模型

Journal of biotechnology & biomaterials Pub Date : 2017-01-01 DOI: 10.4172/2155-952X.1000251

M. Okabe, Toshiko Yoshida, Michiko Suzuki, M. Goto, M. Omori, M. Taguchi, Ayaka Toda, Takuma Suzuki, K. Nakagawa, Fumitaka Hiramoto, T. Ushijima, Hiroki Waki, Etsuko Furuichi, K. Arai, Kaixuan Zhou, Moustafa Fathy Omar, Makoto Nakamura, Y. Nomura, T. Kasama, Kiyoshi Katou, S. Saito, T. Nikaido

{"title":"Hyperdry Human Amniotic Membrane (HD-AM) is Supporting AciclovirIncluded Device of Poly-N-p-Vinyl-Benzyl-D-Lactonamide (PVLA) Spherefor Treatment of HSV-1 Infected Rabbit Keratitis Model","authors":"M. Okabe, Toshiko Yoshida, Michiko Suzuki, M. Goto, M. Omori, M. Taguchi, Ayaka Toda, Takuma Suzuki, K. Nakagawa, Fumitaka Hiramoto, T. Ushijima, Hiroki Waki, Etsuko Furuichi, K. Arai, Kaixuan Zhou, Moustafa Fathy Omar, Makoto Nakamura, Y. Nomura, T. Kasama, Kiyoshi Katou, S. Saito, T. Nikaido","doi":"10.4172/2155-952X.1000251","DOIUrl":"https://doi.org/10.4172/2155-952X.1000251","url":null,"abstract":"The human amniotic membrane (AM) has been widely used in ophthalmic surgery, including in ocular surface reconstruction. However, whether AM is effective for the release of impregnated drugs is unknown. Hyperdry-AM (HD-AM), glutaraldehyde cross-linked HD-AM (Glu HD-AM) and a solvent of poly-N-p-vinylbenzyl- D-lactonamide (PVLA) did not cause irritation to the rabbit eye. Although the concentration of residual glutaraldehyde in Glu HD-AM was approximately 40 ppm (dry-weight basis), no eye irritation was observed. Acyclovir (ACV) containing PVLA-bearing spheres (ACV/PVLA) was loaded into HD-AM (ACV/PVLA HD-AM), and then the therapeutic efficacy of ACV/PVLA HD-AM was compared with that of ACV/PVLA solution in a herpes simplex virus-1 (HSV-1) eye infection model. The former was more effective against ocular infection, and its efficacy was dose- and volume-dependent. Thus, ACV/PVLA HD-AM sheets were very effective as a curative treatment for eye infection with HSV-1. HD-AM can be function as drug delivery system. In the future, it may be possible to combine HD-AM and PVLA in a device that can control the release of hydrophobic medicines.","PeriodicalId":15156,"journal":{"name":"Journal of biotechnology & biomaterials","volume":"8 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82449902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Molecular Imprinted Silica with West Nile Antibody Templates show Specific and Selective Binding in Immunoassays. 分子印迹二氧化硅与西尼罗河抗体模板显示特异性和选择性结合免疫分析。

Journal of biotechnology & biomaterials Pub Date : 2017-01-01 Epub Date: 2017-06-13 DOI: 10.4172/2155-952x.1000260

Julio E Rincon, Fabio Diaz Santillan, Pedro M Palermo Infante, Douglas M Watts, Thomas Boland

{"title":"Molecular Imprinted Silica with West Nile Antibody Templates show Specific and Selective Binding in Immunoassays.","authors":"Julio E Rincon, Fabio Diaz Santillan, Pedro M Palermo Infante, Douglas M Watts, Thomas Boland","doi":"10.4172/2155-952x.1000260","DOIUrl":"https://doi.org/10.4172/2155-952x.1000260","url":null,"abstract":"<p><p>A new molecular imprinting technique was developed for molecularly imprinted polymer particles (MIPs). Particles were synthesized using organic silane chemistries by a sol-gel process, where the relative amount of active monomers was complementary matched to the relative amount of surface charges of the West Nile antibody template. Synthesized MIPs showed specific binding to affinity purified polyclonal West Nile antibodies (WNA) with a loading capacity of 80 µg/mg, while MIPs absorbed non-specific proteins at a loading capacity of 28 µg/mg. A dissociation constant of Kd=57.45 μM was measured from the binding isotherms. MIPs selectively absorbed 27 times more WNA than either albumin or immunoglobulin, while MIPs absorbed 16 times more WNA than non- imprinted particles (NIPs). Finally, fluorescently labeled MIPs were incubated in a high bind 96 well plate previously loaded with template, albumin, or immunoglobulin as an immunoassay test. Fluorescent MIPs significantly bound more to wells with WNA than any other control. Thus, the development of new affordable and robust immunoassays with MIPs would be possible in the future.</p>","PeriodicalId":15156,"journal":{"name":"Journal of biotechnology & biomaterials","volume":"7 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2155-952x.1000260","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37857581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Growth Rate of Pseudomonas aeruginosa Biofilms on Slippery Butyl Methacrylate-Co-Ethylene Dimethacrylate (BMA-EDMA), Glass and Polycarbonate Surfaces. 铜绿假单胞菌生物膜在光滑的甲基丙烯酸丁酯-二甲基丙烯酸乙酯(BMA-EDMA)、玻璃和聚碳酸酯表面的生长速率

Journal of biotechnology & biomaterials Pub Date : 2017-01-01 Epub Date: 2017-11-02 DOI: 10.4172/2155-952X.1000274

Helena Valquier-Flynn, Christina L Wilson, Andrea E Holmes, Christopher D Wentworth

引用次数: 11