利用农业工业残留物生产淀粉酶的青霉菌在食品工业中的应用

N. Arora, Supreet Kaur, Sawinder Kaur
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引用次数: 2

摘要

以农用残渣、米糠、麦麸和香蕉皮为原料,对青霉菌进行固体发酵生产葡萄糖淀粉酶的研究。在不同的农用残渣中,与其他农用残渣相比,经过7天的发酵,米糠的葡萄糖淀粉酶活性(10.29±0.07 Units/ml)、蛋白质含量(1.22±0.0014 mg/ml)和比活性(8.4 Units/mg蛋白质)最高。培养基中添加碳源和氮源可提高酶活性。以蔗糖(13.70±0.77 Units/ml)(1%质量水平)和酵母膏(14.41±0.07Units/ml)(1%质量水平)为碳源和氮源,在90℃、pH 9条件下酶活性最佳。用60%硫酸铵沉淀法对米糠酶进行部分纯化,酶活性最高。青霉菌对米糠马铃薯淀粉的酶解活性最高,为3.69单位/ml。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Use of Agro Industrial Residues for the Production of Amylase by Penicillium sp. for Applications in Food Industry
Glucoamylase production has been investigated by solid state fermentation using agro-industrial residues, rice bran, wheat bran and banana peel by Penicillium sp. Out of the different agricultural residues tested for enzyme production, rice bran yielded the maximum glucoamylase activity (10.29 ± 0.07 Units/ml), protein content (1.22 ± 0.0014 mg/ml) and specific activity (8.4 Units/mg protein) with Penicillium sp. after 7th day of fermentation as compared to other agro residues. Media supplementation with carbon and nitrogen sources enhances the enzyme activity. For maximum production of enzyme, sucrose (13.70 ± 0.77 Units/ml) (1% mass level) and yeast extract (14.41 ± 0.07Units/ml) (1% mass level) as carbon and nitrogen source respectively were found optimum on rice bran with Penicillium sp. Optimum enzyme activity was observed at 90°C, pH 9. The partial purification of enzyme from rice bran by Penicillium sp. was done with 60% ammonium sulphate precipitation showed maximum enzyme activity. Enzyme hydrolysis showed maximum activity (3.69 Units/ml) with potato starch from rice bran by Penicillium sp.
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